查询词典 by-pass

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The Shah Dynasty found then lasted until now. Nepal was invaded by Britain in 1814 and forced to cede a vast expanse of territory to India which was controled by Britain at that time. Its diplomacy was supervised by Britain as well. During the period from 1846 to 1950 Nepal was controled by Rana Family . The fight aganist it intrigued by Nepali Congress brought the dormination of Rana Family to an end, and constitutional monarchy was put into practice. In February of 1959 ,Mahemdra Bir Bikram Shah Dev issued the first constitution of Nepal, the New Constitution was issued in 1962. Birendra , Bir Bikram Shah Dev ascended throne in1972, he dismissed the National Panchayat in the April 16, 1990,and he also issued the third constitution in Nov ember of the same year. Then the multi-party of constitutional monarchy was set up.

建立了沙阿王朝，延续至今。1814年英国入侵，尼泊尔被迫割让大片领土给英属印度，外交受英监督。1846年―1950年为拉纳家族统治时期。1950年11月，尼泊尔大会党等发起反拉纳的斗争，结束了拉纳的统治，并实行君主立宪制。1959年2月马亨德拉颁布尼泊尔第一部宪法。1962年颁布新宪法。1972年比兰德拉国王即位。1990年4月16日，比兰德拉国王解散全国评议会，同年11月颁布第三部宪法，实行君主立宪的多党制。

The outcome measures included muscle spasticity of paretic upper limb by Modified Ashworth Scale, grasp strength of both hands by JAMAR dynamometer, functional performance of paretic upper limb by Action Research Arm Test, daily use and movement quality of paretic upper limb by Motor Activity Log-28, and motor function by Brunnstrom upper limb recovery stage.

疗效评估包含—使用修正式艾许瓦氏等级量表评估患侧上肢肌痉挛程度、使用JAMAR握力计评估双手握力、使用功能研究手臂测试评估患侧上肢功能性表现、使用动作活动日志（Motor Activity Log-28）评估受试者患侧上肢的日常使用量及动作品质、使用布郎斯壮上肢恢复分期（Brunnstrom Upper Limb Recovery Stage）评估患侧上肢的动作功能。

The five paintings include Le Pigeon aux Petits Pois by Picasso, Pastorale by Matisse, The Olive Tree near Estaque by Georges Braque, Woman with a Fan by Amadeo Modligiani, and Still Life with Chandeliers by Fernand Léger.

这五幅画作包括毕加索的《鸽子与豌豆》，马蒂斯的《田园曲》、乔治·布拉克的《埃斯塔克附近的橄榄树》、莫迪格里阿尼《持扇的女人》和莱格尔的《静物·吊灯》。

The evolutionary relationships were investgated by comparing the results from molecular data alone or molecular data combined morphological characters using different analysis methods: maximum parsimony by PAUP(superscript *) 4.0 and Bayesian inference by MrBayes 3.0B4. Base composition and ti/tv of 28S rDNA D2 regions were analyzed by PAUP(superscript *) 4.0. The results showed that GC content of the 28S rDNA D2 gene sequences of subfamily Euphorinae ranges from 40.00% to 49.25%. Transversion frequency of sequence variable sites among species of Euphorinae was higher than that of transition. The subfamily Euphorinae was proved to be a paraphyletic group with close relationship to subfamilies Neoneurinae and Cenocoelinae; only two tribes, Meterorini and Microctonini, were proved to be monophyletic groups; Centistini, Cosmophorini, Euphorini and Dinocampini were paraphyletic groups; the basal position of tribe Meterorini within Euphorinae was partially supported by our phylogenetic trees, and tribe Microctonini was proved to be a group at the top of the trees.

结果表明：优茧蜂亚科的28S rDNA D2基因序列片段的GC%含量在40.00%~49.25%之间变动，而对于碱基替代情况来讲，优茧蜂亚科各个成员间序列变异位点上颠换大于转换；不同的分析和算法所产生的系统发育树都表明目前根据形态定义出的优茧蜂亚科Euphorinae不是一个单系群，而是一个与蚁茧蜂亚科Neoneurinae和高腹茧蜂亚科Cenocoelinae混杂在一起的并系群；在优茧蜂亚科内部，悬茧蜂族Meterorini和食甲茧蜂族Microctonini（排除猎户茧蜂属Orionis）为单系群，而宽鞘茧蜂族Centistini、大颚茧蜂族Cosmophorini、优茧蜂族Euphorini、瓢虫茧蜂族Dinocampini为并系群；悬茧蜂族Meterorini在优茧蜂亚科Euphorinae内位于基部位置的观点得到部分的支持，同时食甲茧蜂族Microctonini被判定为相对进化的类群。

Methods The rabbit model with hyperhomocysteinemia was established by L-methionine subcutaneouse injection. The changes of white microthrombus and microvascular diameter in pia mater microvessel were observed by using cranial window method and closed microcirculation detection system, The concentration of Hcy was detected by high performance liquid, The level of von Willebrand factor, endothelin-1 (ET-1) were assayed by ELISA. The content of NO was measured by nitrate reduction method. Meanwhile, the relationship between the changes of microvascular diameter and blood concentration ET-1, NO was analyzed.

采用L-蛋氨酸皮下注射法建立HHcy家兔模型，应用"颅骨窗口法"和微循环闭路检测系统观察其软脑膜微血管中白微栓数量及微血管口径变化；采用柱前衍生反相高效液相色谱－荧光法检测同型半胱氨酸浓度；平行观测血管内皮功能指标血管性血友病因子抗原（vWF，用酶联免疫吸附法）、血浆内皮素－1ET-1，用ELISA）、血清一氧化氮水平；并对HHcy所致ET-1、NO血液浓度变化和微血管口径变化进行相关分析。

The results show that ponceau 4R excited by light at the wavelength of 330-430 nm can generate a strong fluorescence at the 621 nm peak wavelength with its best excitation wavelength being 376 nm, amaranth excited by light at the wavelength of 300-440 nm can generate a strong fluorescence at the 643 nm peak wavelength with its best excitation wavelength being 370 nm, tartrazine excited by light at the wavelength of 280-380 nm can generate a strong fluorescence at the 565 nm peak wavelength with its best excitation wavelength being 315 nm, sunset yellow excited by light with wavelength of 310-410 nm can generate a strong fluorescence at the 592 nm peak wavelength with its best excitation wavelength being 348 nm, and brilliant blue excited by light at the wavelength of 320-390 nm can generate a strong fluorescence at the 456 nm peak wavelength with its best excitation wavelength being 350 nm.

结果表明，胭脂红在波长330～430 nm的光激发下，产生较强荧光，荧光峰值波长为621 nm，最佳激发波长为376 nm；苋菜红在波长300～440 nm的光激发下，产生较强荧光，荧光峰值波长为643 nm，最佳激发波长为370 nm；柠檬黄在波长280～380 nm的光激发下，产生很强荧光，荧光峰值波长为565 nm，最佳激发波长为315 nm；日落黄在波长310～410 nm的光激发下，产生较强荧光，荧光峰值波长为592 nm，最佳激发波长为348 nm；亮蓝在波长320～390 nm的光激发下，产生较强荧光，荧光峰值波长为456 nm，最佳激发波长为350 nm。

I do not know how the operation and nature of the ancient Patria Potestas can be brought so vividly before the mind as by reflecting on the prerogatives attached to the husband by the pure English Common Law, and by recalling the rigorous consistency with which the view of a complete legal subjection on the part of the wife is carried by it, where it is untouched by equity or statutes, through every department of rights, duties, and remedies.

我们只要回想一下纯粹英国普通法所赋与丈夫的各种特权，并回想一下，在普通法还没有经衡平法或制定法修正的部分中，在权利、义务和救济等各方面严格地坚持妻在法律上必须完全从属的见解，我们将对古代"家父权"的运用和性质，获得一个鲜明的印象。

By applying the optimal tool parameters, the maximum tooth contact pressure is reduced by 45% and the maximum angular position error of the driven gear by 60%, in regard to the spiral bevel gear pair with a pinion manufactured by a cutter of straight-sided prole and of diameter determined by the commonly used methods.

通过应用参数的最佳工具，最大齿接触压力减少了45％，而最高的从动齿轮角位置误差60％，在关于由一个螺旋直齿轮刀具制造了一对锥齿轮双面形象和所常用的方法确定的直径。

To investigate the effect of p38MAPK signal conducting pathway on livercancinoma cell's malignant phenotype induced by VEGF,we take theexperiment with cell growth test,scanning microscope and laser scanningconfocal microscope so that observe effect of p38MAPK signal conductingpathway on liver cancinoma cell growth,pseudopodium formation andframework of cytoskeleton induced by VEGF.results indicate that the cellbecame ellipse and there were more and thick pseudopodium in the cell'ssurface after being treated by VEGF,and destroyed framework ofcytoskeleton,which can be blocked by pretreated with a special inhibitor ofp38MAPK SB203580 so that VEGF promote metastasis by enhancing livercell migration and movement via p38MAPK signal conducting pathway,butVEGF promote cell growth without p38MAPK signal conducting pathway.

为进一步探讨VEGF通过p38信号传导通路诱导肝癌细胞转移时，对肝癌细胞恶性表型的影响，采用细胞增殖实验、扫描电子显微镜、激光扫描共聚焦显微镜观察VEGF对肝癌细胞增殖作用、细胞伪足形成、细胞骨架微丝结构的影响以及p38MAPK信号通路调控作用。结果显示，VEGF能够不通过p38信号通路促进肝癌细胞增殖；VEGF诱导肝癌细胞丝状伪足增多、增长，使细胞骨架微丝结构破坏、甚至消失。阻断p38MAPK信号通路，可以抑制VEGF诱导的肝癌细胞伪足形成，细胞骨架丝状结构呈束状，排列较规整。上述结果表明，VEGF可以通过p38信号传导通路诱导肝癌细胞伪足增多、增长，促使细胞骨架微丝结构破坏，使肝癌细胞迁移、运动能力增加，促进肿瘤转移。VEGF并不通过p38信号通路诱导肝癌细胞增殖作用。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。