查询词典 by-pass line

In stellar spectra, the H - epsilon line ( transition 7-2) is often mixed in with another absorption line caused by ionized

在恒星光谱中，H -ε线(7跃迁至2)经常会与其他的吸收谱线混合，天文学家都知道电离的

The magnesium in rare earth-magnesium intermediate alloy was determined by flame atomic absorption spectrometry with the hyposensitive line of magnesium (202.5 nm) as the analytical line.

以镁的202.5nm次灵敏线为分析线、火焰原子吸收光谱法测定了电解稀土－镁中间合金中的镁含量。

By the new Railway Industry Development Co Ltd duly authorized, Jiangsu Tong Guang-exclusive agent the scope of the new long railway line, railway line scope Ningqi media ads, including the new long-term and a full range of Ningqi Adiantum ads in all stations, railway Cross Highway Bridge and Xinchang Railway advertisement company's existing passenger trains such as the scope and content of ads, authorized a period of from 2007 to 2025, a total of 18 years.

经新长铁路实业发展有限公司正式授权，江苏通广独家代理新长铁路全线范围、宁启铁路全线范围广告媒体发布，包括新长线和宁启铁线全线范围内所有车站广告、铁路跨公路的跨线桥广告及新长铁路公司现有旅客列车广告等范围及内容，授权期限为2007年至2025年，共18年。

F〓 of transgenic allotetraploid fish containing"all-fish"transgene——pCAgcGHc was obtained respectively by inbreeding and gynogenesis. Positive rate of pCAgcGHc detected by PCR was 90％ in F〓 of transgenic allotetraploid fish produced by inbreeding, while most F〓 of transgenic allotetraploid fish produced by gynogenesis showed diploidy measured by flow cytometer. The results above imply that developing the pure line of transgenic allotetraploid fish through inbreeding is feasible, but the operation should be modified if gynogenesis is introduced.

采用近交和雌核发育方法培育出转pCAgcGHc基因异源四倍体鲫鲤F〓代，通过PCR检测，外源基因pCAgcGHc在近交转pCAgcGHc基因异源四倍体鲫鲤F〓中的整合率为90％，而流式细胞技术检测雌核发育转pCAgcGHc基因异源四倍体鲫鲤F〓的倍性多为二倍体，说明通过近交筛选转基因异源四倍体鲫鲤纯系的方法是可行的，但是通过雌核发育来筛选转基因异源四倍体鲫鲤纯系还要就方法上进一步的改进。

The conversion boundary of self-incompatibility was researched by sowing by stage. Sunshine of day and temperature was studied critically by investigating leafage and researching traits indoors. The results showed that the daily minimum temperature was the principal factor of affinity conversion and the affinity conversion boundary was 16.5~18.1℃.HE97 was primarily regard as self-incompatible line in this research. The method was by inbreeding and sib HE97, Half the silk of HE97 and Zheng58. Inbreeding and cross have been done simultaneously. The extending condition of pollen tube was observed by dying the silk after pollinating 4 hours. The individuals with pollen tube were observed having seeds, while the individuals without pollen tube were observed having few seeds or no seeds. So this study regard HE97 as sporophytic self-incompatibility .The planting ration of female parent HE97 and male parent 87-1 was 4:1.The nature cross-pollination was studied by the theory of endosperm xenity, the study show that the setting percentage of HE97 was 625%, in which the seed in self was 70% and the seed in hybrid was 30%. This was consistent with the study of silk anatomy structure, then come to the same conclusion: compatible pollen germination and fertilization can stimulate the self-incompatible pollen germination and fertilization in the same corn cob.

通过分期播种，进行了光温敏自交不亲和材料HE97亲和性转换区间的研究，依据田间跟踪调查叶龄及室内考种研究了HE97亲和性转换区间的光温临界值，结果表明：1日最低温度是影响HE97自交不亲和性的主要原因，亲和性转换区间在16.5℃～18.1℃之间。2通过HE97自交和姊妹交以及将HE97与郑58作为双亲，各自的花丝一分为二，分别正反交，授粉后4个小时分别取其花丝进行固定、染色，观察花粉管的伸长情况，根据结实率与花粉管伸长的对应关系，结果发现观察到花粉管的对应植株收获到了种子，而没有观察到花粉管的对应植株结实率极低或没有结实，本研究把HE97初步划为孢子体自交不亲和类型。3以HE97为母本，87-1为父本，按4母：1父行比，通过花粉直感效应研究天然异交率，结果母本HE97结实良好(62%)，其中自交粒占70%，异交粒占30%，结合花丝切片观察表明，亲和花粉的萌发及受精会导致落在同一果穗柱头上不亲和花粉的萌发不再受抑制。

In another word, the status does not exist that a painter should learn from nature "purely" without any "prejudice" and paint realistically by facing an object to paint out what he or she has perceived. To obtain such capability, painting learners or students shall use their eyes (teachers ask students in the painting class "where are your eyes and your perceptions") to observe objects and things at the beginning of study and then paint the models faithfully by means of "facade-subdivision"(or "preferring to square not round" as described by Xu Beihong, or decomposing the object into many petty facades as described in the line drawing textbook by P.P. Chistjakov so as to turn around the object bit by bit).

也就是说，不存在这样一种状态，画家为了画出自己的感觉，他们应该"纯洁"地不带"偏见"地去向自然学习，去面对物体直截了当地写生，而为了获得这种能力，在学习绘画的初始阶段，就必须训练学生们用自己的眼睛(在绘画课室里，老师总是质问学生，你的眼睛在哪里，你的感觉在哪里)去观察对象，然后用一种"分面"的方法(也就是徐悲鸿所说的"宁方勿圆"，或者按照据说是契斯恰科夫的素描教程的要求把对象分解成许多小面，以便使物体能够一点一点地转过去的方法)把眼前的模特如实地画下来。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Results: Tyroserleutide can significantly increase the life span of H22 tumor-bearing mice by 50-70% in dosages of 20ug/kg/d-80ug/kg/d,specially the high dosage of 80ug/ml can significantly increase the life span by 69.24%; Tyroserleutide can inhibit the growth of transplanted hepatocellular tumor BEL-7402 in nude mice,the rate of tumor inhibition was25-50% in dosages of 40-320ug/ml ,the inhibition rate of 160ng/ml was 44.03%; Tyroserleutide could inhibit the growth of H22 and BEL-7402 tumor in a dose-dependent manner. Simultaneously, tumoricidal activity of tyroserleutide against BEL-7402 cell line in vitro was observed hinger when compared with the control group(P.05).The inhibition effect of 72hrs was higher than 24hrs,48hrs,96hrs.And specially the high dosage of 160ug/ml can significantly inhibit growth of tumor cell by 19.36%. Tyroserleutide can activated PEM and marked enhance cytotoxicity andphagocytosis functions in vitro and in vivo. The OD values of cytotoxicity were observed hinger when compared with the control group(P.05).The cytotoxicity of macrophages activated by tyroserleutide against BEL-7402 and B16-F10 was 35.58%,61.2% in vitro and21.39%,47.63% in vivo. The cytotoxicity rate of nude mice PEM was 32.86%,73.07% in vivo. Furthermore, tyroserleutide alone could stimulated the production of IL-1B TNF- a and NO by M . Tyroserleutide and LPS could synergistically activated M producing more cytotoxicity effectors. Conclusion: Tyroserleutide had inhibition functions against hepatoma carcinoma .Its possible mechanisms were related to the affect that Tyroserleutide could inhibit tumor cell directively and induce tumor cells apoptosis or death effectively.

结果：酪丝亮肽能显著延长腹水型肝癌H_(22)小鼠的生存时间，给药剂量为80μg／kg／d时疗效最显著，达到69.24％，在20μg／kg／d-80μg／kg／d剂量范围内生命延长率为50-70％，给药剂量与荷瘤鼠生存时间呈现一定量效关系；酪丝亮肽能显著抑制人肝癌BEL-7402移植瘤裸鼠的肿瘤生长，给药剂量为160μg／kg／d时疗效最显著，抑制率为44.03％，并且在40-320μg／kg／d剂量范围内抑制率为25-50％，给药剂量与肿瘤抑制率呈现一定量效关系；酪丝亮肽体外对人肝癌BEL-7402细胞生长有一定的抑制作用，在作用72hrs时各浓度酪丝亮肽对肿瘤细胞的抑制作用较24hrs、48hrs、96hrs明显，其中浓度为100μg／ml时抑制率达19.36％；酪丝亮肽体内外均能增强小鼠腹腔巨噬细胞对肿瘤细胞的杀伤：体外作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强，与效应细胞对照组相比有显著性差异(P＜0.05)杀伤率分别达到35.58％、61.2％；体内作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强，与生理盐水对照组相比有显著性差异(P 。05)，杀伤率分别达到21.39%、47.63%；裸鼠腹腔巨噬细胞经酪丝亮肤作用后对BEL一7402、B 16一F10杀伤功能明显增强，与生理盐水对照组相比有显著性差异(P.05)，最高杀伤率分别达到32.86%、73.07%；酪丝亮肤能增强单核巨噬细胞系统的吞噬功能，吞噬指数与生理盐水组比较有显著性差异(P.05)；酪丝亮肤体外作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO，与效应细胞对照组相比有显著性差异(P.05)；酪丝亮肤体内作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO，与生理盐水对照组相比有显著性差异(P.05)；酪丝亮肤能促进鼠巨噬细胞株R戌W264.7分泌合成IL一1p和NO，IL一1日、NO水平分别在酪丝亮肤作用24hrs、12hrs时达到高峰，酪丝亮肤单独应用能提高巨噬细胞的分泌合成功能，而且酪丝亮肤能与LPS协同作用刺激巨噬细胞的细胞毒效应分子分泌合成。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Methods Human monocyte cell line THP-1 was cultured and induced to macrophage by PMA treatment. The effect of 9-cisRA on the differentiation induced by PMA was studied. Cell morphology was observed by phase contrast microscope and the adhesion rate of THP-1 was detected by methyl thiazolyltetrazolium assay. The cell surface markers involved in differentiation of mono cyte/macmphage (CD11b, CD36) were analyzed by flow cytometry.

体外培养人单核细胞系THP-1，经佛波酯诱导分化为巨噬细胞，同时给予9-顺式维甲酸对佛波酯诱导分化进行干预，通过相差显微镜观察细胞形态，MTT比色法检测细胞贴壁率，流式细胞仪检测与单核／巨噬细胞分化有关的细胞表面标志物CD11b和CD36的表达。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。