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The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

The author analyzed total oxygen content, nitrogen content in steel made by typical process and the inclusion composition, change rule, morphology and distribution qualitatively and quantitatively by image analyzer, big sample electrolysis, SEM and energy spectrum analysis. The results showed that final inclusion form was small granule MnS, calcium aluminate, a small quantity of silicate and lumpy Al2O3. By intensifying deoxidation alloying process control in converter, argon blowing and slagging control and desulphuration and calcium reduction operation in refining course and overall-protection casting, the oxygen content was reduced by 20% above averagely, nitrogen content was reduced by about 10% and the inclusion content was reduced by 30%.

通过全O、N分析，夹杂物图像分析仪、大样电解、扫描电镜及能谱分析，定性和定量分析了典型工艺钢种钢中全O、N含量和工序过程夹杂物的构成、变化规律及形态、分布，分析钢中最终夹杂物表现形式主要为小颗粒的MnS、铝酸钙、少量的硅酸盐和块状Al2O3，通过强化转炉脱氧合金化工艺控制、精炼过程吹氩与造渣控制及脱硫和钙处理操作、全程保护浇注，钢水中O含量平均降低20％以上，N含量降低10％左右，铸坯中夹杂物含量降低了30％。

The propose of the research is to insist on the view of applying to solve the various confusion in applying at present when present The Doctrine of Equivalents in China by analyzing dispute in applying of DOE; apply the research method of history analysis and compare synthetically, integrate with the case analysis and draw the conclusion: It is necessary to induct The Doctrine of Equivalents to prevent the tortious people filching the benefit of the inventor and confirm whether the infringed people imitated and copied patent on purpose, it is international tidal current to apply DOE in the processing of patent infringement cognizance, and firstly it must ensure the protecting confine by explaining the claim with compromise explain method, the difference of different extent in patent creation also effect the demarcation of equivalent extension; it should apply the rule of comparing factor one by one rather than comparing the whole equivalent in comparison of equivalents, and needs to be confirmed by increasing the assistant means in the processing of patent infringement cognizance by using Triple Identity Test of Graver; contrast and analyze whether they are equivalent depend on the level of the common technicians in relative domain and it is scientific and reasonable to confirm equivalent time standard by infringement date; in judicial practice of equivalent cognizance, it may use the experiences in Japan for reference to solve the cognizance misunderstanding area of reality in the law and the technology; The superabundance designation that may be understood as a display unfair contract because of neglect and materially misunderstanding should be conditionally applied in current time.

研究目的在于通过分析等同原则在适用上的争议，提出我国适用等同原则时应坚持适用的观点，解决目前在适用中的各种混乱局面；运用历史分析和综合比较的研究方法，结合案例分析；专利侵权认定中引入等同原则是必要的，其实质是防止侵权人窃取发明人的利益，被侵权人是否故意模仿或抄袭专利，不影响等同侵权的判定，运用等同原则判定专利侵权已成为世界性的潮流；适用等同原则首先要对权利要求进行解释以确定保护范围，在解释权利要求时应采取折衷的解释方法，不同专利创造性程度的差异也影响着等同范围的划定；在等同物的比较上，应当适用&逐个要素比较&，而不能适用&整体比较&，在适用&三一致&标准进行侵权认定时，还需要增加辅助手段予以确定；是否构成等同，应当以所属领域普通技术人员的水平来对比分析，以侵权日为等同的时间标准是科学合理的；在等同认定的司法实践中，解决法律事实和技术事实认定上的误区，可以借鉴日本的经验；多余指定可以理解为一个因疏忽大意或重大误解而订立的显失公平的合同，应当在现阶段有条件限制地予以适用。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

Tetrachloroterephthalic acid was prepared by chlorinating terephthalic acid, oleumsolvent and iodine catalyst. Tetrachloroterephthalic acid was acylated by sulphurous oxychloride in the presence of DMF to form tetrachloroterephthaloyl chloride. The acyl chloride product was fluorinated by potassium fluoride and esterified by methanol to synthesize dimethyl tetrafluoroterephthalate. The ester was reduced with potassium borohydride and lithium chloride to give tetrafluoroterephthalyl alcohol. The above product was bromated in the hydrobromic acid to obtain 4-bromomethyl-2,3,5,6-tetrafluoro-benzene methanol. 4-Methyl-2,3,5,-6-tetrafluorobenzene ethanol was obtained by reducing the bromide with magnesium. In the exist of DCC and DMAP, tefluthrin was got by the condensation reaction between 4-methyl-2,3,5,6-tetrafluoro-benzene methanol and-(1RS,3RS)- 3-(2- chloro-3,3,3-trifluoro-1-propenyl)-2,2-dimethyl cycolpropanecar-boxylate acid.

对苯二甲酸与氯气在碘存在下以发烟硫酸为溶剂进行氯化制备四氯对苯二甲酸；再以DMF为催化剂与二氯亚砜反应生成四氯对苯二甲酰氯；以氟化钾为氟化剂，环丁砜为溶剂置换氟化，再与甲醇酯化得到四氟对苯二甲酸甲酯；以KBH4-LiCl为还原剂二甲醚为溶剂反应制得四氟对苯二甲基苄醇；以氯苯为溶剂在溴化氢溶液中溴化得到溴化产物；在乙醇溶液中经镁粉还原得到4-甲基-2，3，5，6-四氟苄醇；甲基苄醇与功夫酸在催化剂二环己基碳二亚胺和4-二甲氨基吡啶作用下缩合得到七氟菊酯，总收率达43.6%。

Results are as followed:1 Exposure of HELF cells to BP caused c-Jun activation,and increased the activity of MAPK,PI-3K,p53 and cyclin D1 pathway.2 BP-induced c-Jun activation was inhibited by dominant negative mutants of extracellular signal-regulated protein kinase or c-Jun NH_2-terminal kinase,but not by p38,impling that JNK and ERK pathways medicate c-Jun activation induced by BP.3 Overexpression of dominant-negative mutants PI-3K and Akt potently blocked phosphorylations of c-Jun and ERK,but not JNK in response to BP,suggesting that PI-3K/Akt pathway positively regulates BP-induced c-Jun activation through ERK.4 Inhibition of p53 by its chemical or molecular inhibitor markedly increased the phosphorylation levels of c-Jun,Akt and ERK upon BP stimulation,indicating that p53 negatively medicates BP-induced c-Jun activation through PI-3K/Akt/ERK pathway.5 The cell lines expressed TAM67 exhibits no significant affecting normal cell growth properties.6 TAM67 was able to significantly block G_1-S transition and subsequent cell proliferation,suggesting that c-Jun is essential for cell cycle alternations elicited by BP.7 Overexpression of TAM67 impaired BP-induced cyclin D1 activation,decreasing expression of E2F1 and pRb,indicating that c-Jun participates in the modulation of BP-induced activation of cyclin D1/pRb/E2F1 pathway.8 Stably expression of TAM67 led to the increases in the expression levels of p53 and p21,elevating phosphorylation level of p53,clearly indicating that c-Jun regulates p53/p21 pathway activation induced by BRCollectively,PI3K/Akt/ERK pathway mediated BP-induced c-Jun activation through p53-dependent mechanism.

结果显示：1BP刺激细胞可促进c-Jun活化，并伴随着MAPK、PI-3K、p53和cyclinD1通路各组成成分的活性增强。2利用MAPK通路的显性失活突变体分别阻断细胞外信号调节激酶和c-Jun氨基末端激酶活性，均可明显抑制BP诱导的c-Jun活化，但阻断p38活性对BP引起的c-Jun活化无明显影响，提示JNK和ERK通路参与调控BP诱导的c-Jun活化。3过表达PI-3K和Akt的显性失活突变体也可显著抑制BP诱导的c-Jun活化，并降低磷酸化ERK的表达水平，但对磷酸化JNK的表达水平无明显影响，说明PI-3K/Akt通路通过ERK正性调控了BP诱导的c-Jun活化。4p53的化学/分子抑制剂能使BP作用的细胞内c-Jun活性明显增加，并同时诱导Akt和ERK的磷酸化水平的升高，表明p53可通过PI-3K/Akt/ERK通路对BP诱导的c-Jun活化进行负性调控。5随后观察转染细胞的生长情况，发现TAM67对细胞正常生长和形态无明显影响。6稳定表达TAM67可有效抑制BP诱导的S期细胞数的增加，提示c-Jun在BP致细胞周期改变的过程中发挥了重要作用。7TAM67过表达能够抑制BP诱导的cyclin D1活化，降低磷酸化Rb以及E2F1蛋白表达水平，表明c-Jun参与调控BP诱导的cyclin D1/Rb/E2F1通路的活化。8过表达TAM67可使BP刺激的细胞中p53、p21总蛋白以及p53磷酸化的表达水平明显升高，可见c-Jun也参与调控BP诱导的p53/p21通路活化。

Results There are 62 patients infected by UU or CT in all of 116 infecund and the can not bear patients(the infections rate is 53.4%). The rate of infected by UU is 42.25. the rate of infected by CT is 17.2%. The rate of infected by both UU,CT is 6.0%. Man's rate of infected by UU ,CT is 35.9%,21.9%. Woman's rate of infected by UU ,CT is

结果 116例不孕不育患者中有62例感染UU或CT（感染率53.4%），UU和CT的感染率分别为42.2%和17.2%，混合感染率6.0%；男性UU和CT感染率分别为35.9%和21.9%，女性UU和CT感染率分别为50.0%和11.5%；女性患者UU和CT的基因拷贝数均高于男性。

True believers may have the assurance of their salvation in various ways shaken, diminished, and intermitted; as, by negligence in preserving of it; by falling into some special sin, which wounds the conscience, and grieves the Spirit; by some sudden or vehement temptation; by God's withdrawing the light of his countenance, and suffering even such as fear him to walk in darkness, and to have no light: So 5:2,3,6 Ps 51:8,12,14 Eph 4:30,31 Ps 77:1-10 Mt 26:69-72 Ps 31:22 Ps 88:1-18 Isa 1:10 yet are they never utterly destitute of that seed of God, and life of faith, that love of Christ and the brethren, that sincerity of heart and conscience of duty, out of which, by the operation of the Spirit, this assurance may in due time be revived, 1Jo 3:9 Lu 22:32 Job 13:15 Ps 73:15 Ps 51:8,12 Isa 1:10 and by the which, in the mean time, they are supported from utter despair.

Mic 7：7，8，9 Jer 32：40 Isa 54：7-10 Ps 22：1 Ps 88：1-18 4 ）真正的信徒可能保证他们的救恩，在以各种方式发生动摇，削弱和intermitted ；作为，疏忽，在维护它；由落入一些特殊的单，其中伤口的良知，并格雷乌斯精神；一些突发或强烈诱惑；上帝的撤回根据自己的国家进行，与苦难，甚至如怕他走在夜色中，并没有轻：那么5：2，3，6的PS 51：8，12，14弗4： 30，31常任77：1-10吨26：69-72聚苯乙烯31：22的PS 88：1-18伊萨1：10 ，但他们从来没有赤贫的种子神的，和生活的信心，而基督的爱和弟兄们，表示真诚的心灵和良知的税，其中，由行动的精神，这样的保证，可在适当时候恢复， 1jo 3时09卢22时32分就业13：15的PS聚苯乙烯73：15 51：8，12伊萨1：10 ，由其中，在平均时间，他们都支持从绝望。

The main viewpoints and model of developing countries MNEs include FDI development cycle put forward by Dunning, the theory of small scale technology put forward by Wells, the theory of localised technological change put forward by Lall, the theory of technological accumulation and industrial upgrade put forward by Tolentino, the model of dynamic comparative advantagr impelling put forward by Aggarwal and Agmon, the model of dynamic competition tactic in technological accumulation put forward by Xian guo-ming and Yang rui.

相对于发达国家工业化早期，发展中国家企业技术能力积累和企业家能力提高具有两方面的有利之处：一是使这种积累和提高由自发过程变为主动过程；二是可以通过政府政策支持对国外相关技术、知识和技能的引进，以及创造更多经济机会和对企业家职能的部分替代或支持，加快企业上述能力积累和提高的过程。

Lupulus, bacteria increased by 0.37% for the 8-year old but decreased by 4. 25%, 10.72% and 18.30% for the 18, 20, and 22-year old plants respectively. The differences were significant except for the 8-year olds. Other respective changes were that actinomycetes decreased by -2.10%, 17.77%, 25.96% and 46.22%, fungi increased by 36.99%, 60.69%, 79.77% and 94.22%, the microbial diversity index increased by 6.12%,-3. 01%,-10.15% and -10.53% and the a-acid increased by 0.44%,-4.53%,-6.86% and -9.49%. The differences were significant except for the 8-year old plants.

与5年植龄的相比，8年植龄的细菌增加了0.37%，而18，20和22年植龄的细菌分别降低了4.25%，10.72%和18.30%，均达到显著差异（除8年外）；各植龄的放线菌分别是5年的102.10%，82.23%，74.04%和53.78%，均达到显著差异；真菌分别是5年的136.99%，160.69%，179.77%和194.22%，差异显著；多样性指数分别是5年的106.12%，96.99%，89.85%和89.47%，前三者均达到显著差异；啤酒花α-酸含量分别是5年的100.44%，95.47%，93.14%和90.51%，均达到显著差异（除8年外）。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。