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Methods: The inhibitation of HL-60 cells by Rapamycin was measured by MTT assay. Morphic change of the apoptosis of HL-60 cells was observed by inverted microscope and light microscope, trapezoid straps by DNA agarose gel electrophoresis, and apoptosis rate by flow cytometry. Western-blot analysis was performed to detect the expression of Bax and Bcl-2 proteins after rapamycin treatment.

用MTT比色法测定雷帕霉素对HL-60细胞增殖的影响;用倒置显微镜和荧光显微镜观测细胞凋亡的形态学改变;DNA琼脂糖凝胶电泳观测凋亡的梯形条带;流式细胞仪检测细胞凋亡率;Western-blot检测HL-60细胞经雷帕霉素处理后,Bax、Bcl-2蛋白的表达情况。

Evaluation Information does not include, however, information that is or becomes generally available to the public other than as a result of a disclosure by Buyer or its Representatives, was available to Buyer on a nonconfidential basis prior to its disclosure by Seller or its Representatives, becomes available to Buyer on a nonconfidential basis from a person, other than Seller or its Representatives, who is not known by Buyer to be otherwise bound by a confidentiality agreement with Seller or to be otherwise under an obligation to Seller not to transmit the information to Buyer, or was independently developed by Buyer without reference to the Evaluation Information.

评估信息不包括,然而,信息供给或变得一般公众除由于透露之外由买家或它的代表,供给买家根据一个没有争议的事物。依据在它的透露之前由卖家或它的代表,变得可利用对买家根据一个没有争议的事物。依据从人,除卖主之外或它的代表,由卖家不知道由一个机密协议否则一定以卖主或有义务否则对卖主不传送信息给买家,或由买家独立地开发了没有在评估信息的参考。

The results calculated by the Elovich rate equation showed that K release rates from 1 mol.L-1 HNO3 extraction >those from 2 mol.L-1 HNO3 extraction >those from H-resin extraction. Elovich equations combining with the data of H-resin and 2 mol.L-1 HNO3 successive extraction could well described the release characteristics of soil K. The correlation coefficients of the equations reached most significant levels (r=0.956**—0.999**), and standard errors of specific K release capacities between the vaules calculated by the equations and those measured by both successive extractions were smaller(s=1.61 and 3.52). The specific release rates of soil K calculated by the Elovich rate equations were significantly correlated with the indices of soil K availability (the amounts of easily available K, nonexchangeable K, total available K in soils and K absorbed by ryegrass).

研究结果表明:1 mol.L-1 HNO3提取法的释K速率>2 mol.L-1 HNO3提取法的释K速率>氢饱和交换树脂提取法的释K速率;氢质交换树脂和2 mol.L-1 HNO3提取法结合Elovich模型较理想地描述了土壤K素的释放特性,拟合方程的相关系数均达极显著水平(r=0.956**-0.999**),土壤特征累积释K量的计算值与实测值的标准差较小(s=1.61和3.52),且通过速率方程计算的土壤特征释K速率与K素有效性指标(速效K、缓效 K、有效K及黑麦草总吸K量)的相关性也较为密切。

Mg/ kg way partially inhibited by L- NAME,or by sodium orthovanadate(50 ug/ kg),a potent inhibitor of protein tyrosine phosphatase;(2)genistein also decreased the PP of renal vascular bed in a dose-dependent manner and the effect of genistein was completely inhibiteby pretreatment with sodium orthovanadate,but unaffected by L- NAME;and(3 genistein decreased the PP of mesenteric vascula bed in a dose-dependent manner,an effect which was partially inhibited by sodium orthovanadate,but unaffected by L- NAME .

mg/ kg引起的效应;(2)向肾血管灌注环路中直接注射GST也可剂量依赖性地降低肾动脉的灌流压,预先注射正钒酸钠可完全抑制GST引起的效应,而L- NAME对此效应没有影响;(3)肠系膜血管灌流环路中注射GST可剂量依赖性地降低其灌流压,这一效应可被正钒酸钠部分抑制,而L- NAME对此无影响。

MAIN OUTCOME MEASURES: The morphology of BMSCs was observed by the inverted phase contrast microscope. Expression of BMSC surface antigens was detected by flow cytometer. Osteogenous potential was assessed by alkaline phosphatase staining. Adipogenic potential was evaluated by Oil red O staining. Chondrogenic potential was assessed by toluidine blue staining.

主要观察指标:倒置相差显微镜下观察骨髓间充质干细胞的生长状态,流式细胞仪检测细胞表面标志物的表达,采用碱性磷酸酶染色鉴定成骨能力,以油红O染色鉴定成脂能力,以甲苯胺蓝染色鉴定成软骨能力。

Umbrella form inflorescence normally 2~4 bunch gives birth to short branch of Yu Xie armpit, short branch grows 1~2 millimeter; Inflorescence total stalk grows 5~10 millimeter, by short fluff; Bud piece 4, wide egg form, have 5~6 arteries and veins, outside a few without wool, inside face by white short fluff; Inflorescence of each umbrella form has beautiful 4~6, the leaf is opened or open at the same time with the leaf first; Peduncular grow about 1.5 millimeter, close by white fluff; Perianth piece 6, elliptic, two sides by short fluff; In male flower can Yo is staminal 9, filament has grey yellow fluff, form of heart of shape of aegis of body of gland of ministry of the 3rd round of stamen radical, do not have a handle nearly, degrade pistil is not had; Stamen degrades in female flower 9, staff state, by grey yellow fluff, ovarian crock form, style is dumpy, post head shape, shallow crack.

伞形花序通常2~4个簇生于叶腋短枝上,短枝长1~2毫米;花序总梗长5~10毫米,被短柔毛;苞片4,宽卵形,有5~6条脉,外面几无毛,内面被白色短柔毛;每一伞形花序有花4~6朵,先叶开放或与叶同时开放;花梗长约1.5毫米,密被白色柔毛;花被片6,椭圆形,两面被短柔毛;雄花中能育雄蕊9,花丝有灰黄色柔毛,第三轮雄蕊基部腺体盾状心形,近无柄,退化雌蕊无;雌花中退化雄蕊9,棍棒状,被灰黄色柔毛,子房壶形,花柱粗短,柱头头状,具浅裂。

The method includes the steps of forming a peelable resin layer on a silicon substrate, forming the wiring substrate on the peelable resin layer, mounting semiconductor chips on the wiring substrate, forming semiconductor devices by sealing the plurality of semiconductor chips by a sealing resin, individualizing the semiconductor devices by dicing the semiconductor devices from the sealing resin side but leaving the silicon substrate, peeling each of the individualized semiconductor devices from the silicon substrate between the silicon substrate and the peelable resin layer, and exposing terminals on the wiring substrate by forming openings through the peelable resin layer or by removing the peelable resin layer.

该方法包括以下步骤:在硅衬底上形成可剥离树脂层;在所述可剥离树脂层上形成布线衬底;将半导体芯片安装在所述布线衬底上;通过用密封树脂密封所述多个半导体芯片来形成半导体器件;通过从密封树脂侧将这些半导体器件切分但是保留硅衬底来使这些半导体器件个体化;将每个个体化半导体器件在所述硅衬底和可剥离树脂层之间从硅衬底上剥离;并且通过形成穿过可剥离树脂层的孔或者通过除去可剥离树脂层来使布线衬底上的端子暴露。

The causes were right auricle injury by violently placing a electric catheter to it (n=2),coronary sinusinjury by violently placing a electric catheter to distal CS (n=3) and by intracardiac electric defibrillation using electric catheter electrodes of high right atrium and CS as current loop(n=1),perforations of right atrial wall (n=3) and left atrial wall(n=2) by sliding up of the transseptal set,and perforation of left auricle by misplacing the mapping catheter of left superior pulmonary vein to it (n=1). 10 cases of acute tamponade occurred during operation, and 2 after operation.12 patients were treated successfully with urgent pericardiocentesis, and no patients required emergency surgery.

引起心包填塞的原因有:①放置电极导管至右心耳时用力过大致右心耳损伤(2例);②放置电极导管至冠状静脉窦时,因导管不易进入远端而用力过大致CS损伤(3例);③心内电除颤时采用高位右房和CS电极作回路,致CS损伤(1例);④房间隔穿刺系统向上滑动穿破右房(3例)和左房(2例);⑤放置左上肺静脉标测电极时导管误入左心耳致其穿孔(1例)。10例急性心包填塞出现于术中,另2例术后发生。12例患者经紧急心包穿刺引流均成功治愈,无1例需外科急诊手术。

The causes were right auricle injury by violently placing a electric catheter to it (n=2),coronary sinusinjury by violently placing a electric catheter to distal CS (n=3) and by intracardiac electric defibrillation using electric catheter electrodes of high right atrium and CS as current loop(n=1),perforations of right atrial wall (n=3) and left atrial wall(n=2) by sliding up of the transseptal set,and perforation of left auricle by misplacing the mapping catheter of left superior pulmonary vein to it (n=1). 10 cases of acute tamponade occurred during operation, and 2 after operation.12 patients were treated successfully with urgent pericardiocentesis, and no patients required emergency surgery.

引起心包填塞的原因有:①放置电极导管至右心耳时用力过大致右心耳损伤(2例);②放置电极导管至冠状静脉窦时,因导管不易进入远端而用力过大致CS损伤(3例);③心内电除颤时采用高位右房和CS电极作回路,致CS损伤(1例);④房间隔穿刺系统向上滑动穿破右房(3例)和左房(2例);⑤放置左上肺静脉标测电极时导管误入左心耳致其穿孔(1例)。10例急性心包填塞出现于术中,另2例术后发生。12例美白患者经紧急心包穿刺引流均成功治愈冬季,无1例需外科癌症急诊预防手术胃痛。

This conclusion is based on three groups of experiments with detached root tips of wheat seedlings: By following the time course changes of NO synthase , superoxide synthase and ABA accumulation under dehydration stress, we found that ABA accumulation was preceeded by the changes of NOS and superoxide synthase. ABA accumulation induced by dehydtrion stress and osmotic stress can be blocked by NO scanvegers and NOS inhibitors, while only dehydration stress-induced ABA accumulation can be blocked by ROS scanvegers. Exogenous applied NO or ROS induced ABA accumulation in root tips of wheat seedlings, and synergistic effects were found between NO and NO. The in planta expereiments also proved that ROS and NO are involved in water stress-induced ABA accumulation.Ⅱ.

证据主要来自以离体根尖为材料的实验:(1)通过检测干旱胁迫下小麦根尖NOS活性、O〓合成酶活性和ABA积累的时间变化进程,发现ABA积累滞后于ROS和NO的变化;(2)抑制剂实验表明,NO清除剂和NOS抑制剂可以抑制自然干旱胁迫及渗透胁迫诱导的小麦根尖ABA积累,ROS清除剂抑制自然干旱胁迫诱导的ABA积累而不抑制渗透胁迫诱导的小麦根尖ABA积累;(3)在非胁迫条件下施加外源的ROS或NO可以诱导小麦根尖积累ABA,ROS和NO之间具有协同作用。

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