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The results show that ponceau 4R excited by light at the wavelength of 330-430 nm can generate a strong fluorescence at the 621 nm peak wavelength with its best excitation wavelength being 376 nm, amaranth excited by light at the wavelength of 300-440 nm can generate a strong fluorescence at the 643 nm peak wavelength with its best excitation wavelength being 370 nm, tartrazine excited by light at the wavelength of 280-380 nm can generate a strong fluorescence at the 565 nm peak wavelength with its best excitation wavelength being 315 nm, sunset yellow excited by light with wavelength of 310-410 nm can generate a strong fluorescence at the 592 nm peak wavelength with its best excitation wavelength being 348 nm, and brilliant blue excited by light at the wavelength of 320-390 nm can generate a strong fluorescence at the 456 nm peak wavelength with its best excitation wavelength being 350 nm.

结果表明,胭脂红在波长330~430 nm的光激发下,产生较强荧光,荧光峰值波长为621 nm,最佳激发波长为376 nm;苋菜红在波长300~440 nm的光激发下,产生较强荧光,荧光峰值波长为643 nm,最佳激发波长为370 nm;柠檬黄在波长280~380 nm的光激发下,产生很强荧光,荧光峰值波长为565 nm,最佳激发波长为315 nm;日落黄在波长310~410 nm的光激发下,产生较强荧光,荧光峰值波长为592 nm,最佳激发波长为348 nm;亮蓝在波长320~390 nm的光激发下,产生较强荧光,荧光峰值波长为456 nm,最佳激发波长为350 nm。

By applying the optimal tool parameters, the maximum tooth contact pressure is reduced by 45% and the maximum angular position error of the driven gear by 60%, in regard to the spiral bevel gear pair with a pinion manufactured by a cutter of straight-sided prole and of diameter determined by the commonly used methods.

通过应用参数的最佳工具,最大齿接触压力减少了45%,而最高的从动齿轮角位置误差60%,在关于由一个螺旋直齿轮刀具制造了一对锥齿轮双面形象和所常用的方法确定的直径。

To investigate the effect of p38MAPK signal conducting pathway on livercancinoma cell's malignant phenotype induced by VEGF,we take theexperiment with cell growth test,scanning microscope and laser scanningconfocal microscope so that observe effect of p38MAPK signal conductingpathway on liver cancinoma cell growth,pseudopodium formation andframework of cytoskeleton induced by VEGF.results indicate that the cellbecame ellipse and there were more and thick pseudopodium in the cell'ssurface after being treated by VEGF,and destroyed framework ofcytoskeleton,which can be blocked by pretreated with a special inhibitor ofp38MAPK SB203580 so that VEGF promote metastasis by enhancing livercell migration and movement via p38MAPK signal conducting pathway,butVEGF promote cell growth without p38MAPK signal conducting pathway.

为进一步探讨VEGF通过p38信号传导通路诱导肝癌细胞转移时,对肝癌细胞恶性表型的影响,采用细胞增殖实验、扫描电子显微镜、激光扫描共聚焦显微镜观察VEGF对肝癌细胞增殖作用、细胞伪足形成、细胞骨架微丝结构的影响以及p38MAPK信号通路调控作用。结果显示,VEGF能够不通过p38信号通路促进肝癌细胞增殖;VEGF诱导肝癌细胞丝状伪足增多、增长,使细胞骨架微丝结构破坏、甚至消失。阻断p38MAPK信号通路,可以抑制VEGF诱导的肝癌细胞伪足形成,细胞骨架丝状结构呈束状,排列较规整。上述结果表明,VEGF可以通过p38信号传导通路诱导肝癌细胞伪足增多、增长,促使细胞骨架微丝结构破坏,使肝癌细胞迁移、运动能力增加,促进肿瘤转移。VEGF并不通过p38信号通路诱导肝癌细胞增殖作用。

But as my mother often said, ''They asked for trouble'' —by the way they make their arguments, by their puzzlingly shoddy scholarship, by what they emphasize and de-emphasize, by what they leave out and by writing on this sensitive topic without doing extensive interviews with the lobbyists and the lobbied.

他们制造的这些争议,他们偷换的概念,他们强调的和忽略的,他们轻率的未经调查访问就提出的那些关于游说组织的敏感论调,所有这些就像我妈妈说的那样,"他们在自找麻烦"。

In a randomized, placebo-controlled study performed on 80 patients with chronic LBP, Revel et al.88 identified seven variables associated with a positive response to facet joint anesthesia: age greater than 65 yr and pain not exacerbated by coughing, not worsened by hyperextension,not worsened by forward flexion, not worsened when rising from forward flexion, not worsened by extension–rotation, and well-relieved by recumbency.

在一项80例慢性LBP患者的随机安慰剂对照试验中,Revel等鉴定出七种小关节麻醉阳性相关的因素:年龄大于65岁,咳嗽时疼痛不加剧,过度伸展不加剧,从前屈变伸直时不加剧,伸展旋转时不加剧,卧位时缓解。

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异;采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异;采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry,FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时,对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后,Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR,RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时,Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope,LSM)观察北豆根总碱(0、40μg/ml)作用24小时后,Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

David Copperfield by Dickens, Rouge et Noir by Stendhal, History Study by Toynbee, Sea Wolf by Jack London, the philosophy of existentialism, and the novels by Merimee, etc. have affected the life of Wang Shi quite a deal.

狄更斯的《大卫·科波菲尔》,司汤达的《红与黑》,汤因比的《历史研究》,杰克·伦敦的《海狼》,存在主义哲学和梅里美的小说等都对王石的生活产生过重要影响。

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h,MTT法检测ox-LDL对RAW264.7细胞生长的影响;流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡;用特异性siRNA沉默Daxx在RAW264.7细胞中的表达,通过AO/EB染色观察基因沉默后的细胞凋亡形态学改变;高效液相色谱检测细胞内胆固醇含量;油红O染色观察细胞内脂滴的形成情况;Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况;用Western Blot印迹法检测caveolin-1蛋白的表达。

Resolved, therefore, That, being invested by the Creator with the same capabilities, and the same consciousness of responsibility for their exercise, it is demonstrably the right and duty of woman, equally with man, to promote every righteous cause, by every righteous means; and especially in regard to the great subjects of morals and religion, it is self-evidently her right to participate with her brother in teaching them, both in private and in public, by writing and by speaking, by any instrumentalities proper to be used, and in any assemblies proper to be held; and this being a self-evident truth, growing out of the divinely implanted principles of human nature, any custom or authority adverse to it, whether modern or wearing the hoary sanction of antiquity, is to be regarded as self-evident falsehood, and at war with the interests of mankind.

因此,决议认为,由于造物主赋予男女相同的能力和运用这些能力的相同的责任感,所以男女显然都有相同的权利和责任,利用一切正义的手段促进一切正义的事业;尤其在道德和宗教这些重大的问题上,不言而喻,妇女有权同她的兄弟一道,在私下和公开的场合,通过文章和演讲,运用任何合适的手段、在任何合适的集会上,开宗名义地宣讲。这是不言自明的真理,它是从扎根在人类本性的神圣原则中产生的,任何与之相违背的习俗和权威,不论是现代的还是被磨损得苍白的古老法则,都将被视作不言而喻的谬误,是违背人类利益的。

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年美国个人破产法的一个改动使得破产登记急速下降,而后引起了信用卡大规模的亏损。

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Lao Qiu is the Chairman of China Qiuyang Translation Group and the head master of the Confucius School. He has committed himself to the research and promotion of the classics of China.

老秋先生为中国秋阳翻译集团的董事长和孔子商学院的院长,致力于国学的研究和推广。