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Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

The sizes of the self-assembly micelles of the amphiphilic block copolymers and the effects werestudied by dynamic light scattering and UV-visible spectrophotometer. The micelle sizes werelargely influenced bythe hydrophobic chain content in the copolymer, the properties of the solvent and the copolymer concentration in the organic phase. The morphology of the micelles was investigated by transmission electron microscopy. Theresults showed that the micelles appeared spheres with inner core and outer shell. The critical association concentrations were determined by pyrene monomer fluorescence probe technology. The degradability of copolymer was studied by the loss of the intrinsic viscosity in the degradation process. The results indicated that the degradation rate of PECL was slower thanthat of PELLA and PEDLLA. The stability of the copolymer micelle dispersion was examined by measuring the critical flocculation concentration, which gradually decreased with increasing the content of thehydrophobic chain in the copolymer. The rheological results showed thatthe viscosity ofthe micelle dispersion with higher content of PEG segments was firstly decreased and then increased with the increasingthe temperature. Otherwise, the variation of viscosity was irregular. The viscosity of the micelle dispersion increased with the increase of the electrolyte (Na 2SO 4) concentrations. Polymer micelles paclitaxel was prepared by self-emulsification/solvent evaporation method and solid dispersion technique with amphiphilic block polymers as the carrier material.

运用动态光散射和紫外分光光度计研究了两亲性嵌段共聚物自组装胶束的粒径及其影响因素，结果表明，自组装胶束的形成机理受制备方式的控制，胶束的粒径随着共聚物相对分子质量或疏水嵌段相对分子质量和有机相中共聚物浓度的增大而增大，随着有机溶剂的水溶性的增强而减小；采用透射电镜观察胶束的形态结构，发现胶束呈具有核壳结构的均匀球形；采用芘荧光探针法测两亲性嵌段共聚物的临界聚集浓度；通过降解过程中共聚物特性粘度的变化研究两亲性共聚物的降解性能，发现与PELLA和PEDLLA的相比，PECL 降解速率较慢；随着共聚物中疏水嵌段含量的增大，纳米分散液的稳定性逐渐下降；PEG含量高的PEDLLA纳米分散液随着温度的升高，纳米分散液的粘度先下降然后增大，反之，没有规律性，且随着电解质Na 2SO 4浓度的增大，体系粘度增大。

For preparing for the researches of text digital watermarking and advantaging other researchers" development when using the Chinese mathematical expression theory, this paper designs and realizes a development platform of Chinese mathematical expression. It has been proved by experiments that the platform"s function, execution speed and stability can satisfy the requirements of conversion and operation of enormous data.This research found out that the structure information of Chinese have plenty redundant information that can be used by the Chinese mathematical expression for the digital watermarking, the data embedded can possess better robustness. Based on this finding, this paper designs a kind of text digital watermarking method of embedding watermarking by using Chinese structure, and realizes the method by using the Chinese mathematical expression platform.Considering the watermarking embedding algorithms importance to the robustness of the text digital watermarking, this paper makes fully use of the advantage of the Chinese mathematical expression theory to design a kind of layered text method, which has being realized by using the Chinese mathematical expression platform. The layered text method can handle the text as a solid structure, and the embedding data havepreferable robustness.Finally, based on the researches of the text digital watermarking method and the text layered method, this paper integrates the method of making use of the Chinese structure embedding watermarking with the robust algorithm based on text layered, and realizes a brand-new text digital watermarking method. Proved by lots of experiments, this brand-new method has good robustness and better invisibility.

为了为研究工作做准备和方便其他使用汉字数学表达式理论体系的人进行开发，本文基于汉字数学表达式理论设计实现了一个汉字数学表达式平台，实现了Unicode汉字集中的汉字、汉字数学表达式、汉字的Unicode码之间的相互转化、依据汉字数学表达式提取汉字的结构信息、由汉字数学表达式在非中文平台上不依赖字库显示汉字的图像等功能，使用复合文档技术和流压缩技术进行数据的存储，使用对汉字数学表达式进行二叉树拆分来处理汉字数学表达式中包含的信息，使用自定义哈希函数来提高运行速度，实验证明，该平台功能、速度和稳定性完全能满足大量数据的转化和运算要求；通过研究表明汉字的结构信息中存在大量可使用汉字数学表达式理论加以利用的水印加载空间，加载在该空间的数据可以具有较好的鲁棒性，基于此，本文设计了一种利用汉字结构加载水印的文本数字水印方法，并使用汉字数学表达式平台将其实现；由于水印嵌入算法对于文本数字水印的鲁棒性至关重要，本文充分利用汉字数学表达式理论的优点，设计了一种文本分层的方法，并使用汉字数学表达式平台将其实现，使文本成为一个立体的空间，使嵌入在其中的数据具有更好的鲁棒性；最后在基于汉字数学表达式的文本数字水印嵌入方法的研究和基于汉字数学表达式的文本分层方法的研究的基础上，结合利用汉字结构嵌入水印的方法和基于文本分层的抗攻击文本水印嵌入算法实现了一种全新的文本数字水印方法，经实验证明，该方法有良好的鲁棒性和较好的隐蔽性。

A it is predominantly derived from the initial variety or from a variety which itself was predominantly derived from the initial variety, while retaining the expression of essential characteristics that result from the genotype or combination of genotypes of the initial variety; an essentially derived variety may have been obtained by the selection of a natural or induced mutant or of a somaclonal variant, by the selection of a variant individual from plants of the initial variety, by backcrossing, by a transformation by genetic engineering, or by any other similar method

一个品种主要由原始品种演化而来，或主要由原始品种的实质性派生品种演化而来，同时保留了来自原始品种基因型或基因型组合的基本特征的表达；一个实质性派生品种可能通过选择自然变异或诱变或体细胞无性变异、原始品种植株的个体变异的选择、回交、基因工程的改造或任何其它类似方法而获得。

Not by rules and observances, not even by much learning, nor by gain of absorption, nor by a life of seclusion, nor by thinking,"I enjoy the bliss of renunciation, which is not experienced by the worldling" should you, O monks, rest content, until the utter destruction of cankers is reached.

比丘不应只是因为有戒行、或多闻、或有禅定、或独处、或自知&我得享凡夫享受不到的出离乐&而感到满足，而不灭尽烦恼。

The steps were as follows: Acquiring superant from the culture medium by centrifugation, discarding waste residue by filtration, concentrating by hollow-fiber membrane, treating with salting-out, dialyzing the product of salting-out, treating the dialysate by vaccum freeze dryer, dissolving solid , using the method of gel filtration chromatography with Sephadex G-100/G-75, and proof-test by filter-paper chromatography ,then preparation by thin-layer chromatography was carried out.

建立了分离纯化该物质的有效途径：发酵液离心除菌→抽滤除杂→中空纤维过滤浓缩→盐析→透析→真空冷冻干燥→溶解→交联葡聚糖凝胶Sephadex G-100凝胶分离→交联葡聚糖凝胶Sephadex G-75凝胶分离→纸层析检验→薄层层析制备。

In this paper, based on the theory of history course resource and by using traditional researching methods of "testifying history by arts""bilateral connection of arts and history", the way to construct the teaching model by combination of liberal arts and history, to analyze the literature in junior high school Chinese course (including Chinese textbooks, after school reading books and recommended masterpiece at home and abroad) to enrich the history teaching by inosculating the literature content in junior high school Chinese lesson into history teaching were explored in order to realize the resource sharing in the Chinese and history teaching and to achieve the state of "testifying history by the help of arts""explaining the arts by the help of history" and " inosculating arts into history".

本研究尝试根据历史课程资源理论和传统的&以文证史&、&文史互通&的史学研究方法，探索在初中历史教学实践中构建文史综合的教学模式，研究如何从课程资源的角度解读中学语文课程（包括语文教材、课外读本和推荐阅读中外名著）中的文学作品，将中学语文课程中的文学作品作为不可或缺的实证史料充实进历史课程，融入历史教学，实现初中历史与语文课程的资源共享，做到&以文证史&、&以史释文&、&融文入史&。

This paper discussed the publishing right posed by network transmission of data,reproduction right posed by digital technology,a new database right posed by information packaging,copyright enforcement posed by downloading and printing out,fair use posed by virtual library,international copyright posed by global network communication,and proposed schemes for salving these problems.

本文着重讨论了数据的网络传输引起的发表权、数字化技术引起的复制权、数据包装技术引起的数据库权、下载与印出引起的版权行使、虚拟图书馆引起的合理使用、全球通讯引起的跨国版权问题，以及我国在建立信息高速公路过程中解决知识产权问题的对策。

Highlights from this year's festival included Tan Dun's boldly original new opera Marco Polo, two programmes by the Montreal Symphony Orchestra, a new dance piece by Pina Bausch inspired by Hong Kong, the acclaimed production of A Midsummer Night's Dream by the Royal Shakespeare Company, L'Allegro, Il Penseroso ed il Moderato by the Mark Morris Dance Group and Chess Man by Beijing's Central Experimental Theatre.

本年精彩节目包括谭盾的原创歌剧&马可波罗&、蒙特利尔交响乐团的两个演奏会、翩娜。包殊取材自香港的新编舞蹈作品、皇家莎士比亚剧团上演举世推崇的&仲夏夜之梦&、马克。莫里斯舞团的&欢笑、忧伤、悲喜之间&，以及中央实验话剧院的&棋人&。

Qingdao Economic and Technological Development Area has formed industrial groups at the core of Shandong Peninsula Manufacturing Base: electric home appliances and electronic industries represented by Hai'er, Hisense and Aucma; petrochemical industry led by the Large Petroleum Refinery Project and represented by Kohap chemical fibre, Lidong chemical industry, SK chemical industry; machinery industries represented by Haixi Bay Ship Building and Repairing Base, Federal Mogul piston, Pohang stainless steel; material industry represented by Alum, Jinjing Glass, Saint-Gobain Glass; international trade storage and logistics industries represented by Maersk logistics, Itochu international logistics and Hanjin shipping.

青岛开发区形成了作为胶东半岛制造业基地核心区的产业集群环境：以海尔、海信、澳柯玛等为代表的家电电子产业集群；以大炼油项目为龙头、高合化纤、丽东化工、 SK 化工等为代表的石化产业集群；以海西湾造修船基地、辉门活塞、浦项不锈钢等为代表的机械产业集群；以美铝、金晶玻璃、圣戈班玻璃等为代表的材料产业集群；以马士基物流、伊藤忠国际物流、韩进海运等为代表的国际贸易仓储物流产业集群。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。