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Linear solvation energy relation was firstly introduced in the paper, and the method and step of gaining the special parameters of solvent and solute were introduced in detail, and these special parameters were used to selection and optimization of extractive agent in extractive distillation. The model of predicting activity coefficient at infinite dilution was established by linear solvation energy relation. By the above theory, several candidate solvents were selected to separate C5 fraction, and by experiment of vapor-liquid phase equilibrium at atmosphere pressure, the mixture of N-methyl-pyrrolidone including 4% water was used extractive agent of separating C5 fraction. Then binary vapor-liquid phase equilibrium of the some compound in C5 fraction and N-methyl-pyrolidone were determined at atmosphere pressure and correlated by NRTL equation. Isoprene being objective compound, experiments were operated in the laboratory-scale column; the processes were simulated by RadFrac model in ASPEN PLUS. And by the combination of experiment and simulation, the new process of separation of C5 was established.

本文首先对线性溶剂化能关系进行了分析介绍，也介绍了采用溶剂化能关系得到溶质溶剂特性参数的方法步骤，并将这些参数用于萃取精馏萃取剂的选择与优化；也通过线性溶剂化能关系式建立无限稀释活度系数预测模型，取得了一定的预测精度；将通过上述方法初选的溶剂进行了常压汽液平衡的测定研究，通过实验研究选定含水4%的N-甲基吡咯烷酮溶液做为C5分离的萃取剂，测定了大量N-甲基吡咯烷酮与C5组分的二元平衡数据，并进行关联计算；以异戊二烯为目标产物在实验室规模的精馏塔内进行了C5分离的实验研究，取得大量塔内数据；通过选用合适的热力学模型，采用ASPEN中RadFrac模块对分离过程进行模拟研究，通过实验与模拟相结合，建立了C5分离的新工艺，为将来的工业化打下了坚实的基础。

Major content of this work includes five aspects as follows:(1). Oxygen sensitive materials had been prepared by carrier covalence method, and the preparation mechanism of the materials had been investigated by FI-IR, SEM, and the detection experiment of oxygen. Then through the detection of oxygen, the sensitivity and stability had been estimated, and it was studied that the properties of materials would be affected by the various factors.(2). Oxygen sensitive materials had been prepared by carrier covalence-cross linking method, and the preparation mechanism of the materials had been investigated by FI-IR, SEM, and the detection experiment of oxygen. Then through the detection of oxygen and spectrophotometer, the sensitivity and stability had been estimated, and it was studied that the properties of materials would be affected by the various factors.(3). For the detection of gaseous oxygen, the fiber optical sensor on basis of oxygen sensitive materials had a respond time of 10s, detection limit of 5ppmand detection precision of 0.5%. The sensor had good repeatability and stability, a less delay and at least 1 year life-span.

本论文主要包括以下五个方面的内容：(1)载体共价法制备氧敏感材料：通过红外光谱、扫描电镜图谱和氧测试实验探讨该氧敏感材料的制备机理，通过氧测试实验评价该氧敏感材料的氧敏感性和稳定性，同时研究了各种因素对该氧敏感材料性能的影响(2)载体共价—交联法制备氧敏感材料：通过红外光谱、扫描电镜图谱和氧测试实验探讨该氧敏感材料的制备机理，通过氧测试实验和分光光度计评价该氧敏感材料的氧敏感性和稳定性，同时研究了各种因素对该氧敏感材料性能的影响(3)氧敏感材料在光纤气态氧传感器中的应用：该传感器的响应时间为10s，检测下限为5ppm，检测精度为0.5％，具有较好的重复性和稳定性，迟滞较小，使用寿命至少为1年，适合各种环境下气态氧浓度的检测。

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制备SD大鼠肝纤维化动物模型，连续灌胃6周，处死动物取血，检测血清ALT、AST、ALB；ELISA法测定血清透明质酸；取肝、睥，并计算肝、脾指数，组织切片HE染色后进行组织病理学疗效评估；用SP免疫组化法测定肝组织TGF-β1、PPAR-γ及PDGF蛋白表达；半定量RT-PCR方法检测肝组织TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γ和iNOSmRNA的表达。

The rubber composition for covering a carcass cord of the present invention comprises 25 to 80 parts by mass of silica, 1 to 15 parts by mass of a silane coupling agent and 3 to 30 parts by mass of an organic vulcanizing agent represented by the following general formula (1):- n - wherein R is -(CH 2 -CH 2 -O) m -CH 2 -CH 2 -; x is an integer of 2 to 6; n is an integer of 10 to 400; and m is an integer of 2 to 5, based on 100 parts by weight of a diene rubber, and a tire prepared by using the rubber composition.

以100重量份的二烯橡胶为基准，本发明的用于包覆胎体帘线的该橡胶组合物包含25～80质量份的二氧化硅、1～15质量份的硅烷偶联剂和3～30质量份的由如下化学式(1)表示的有机硫化剂：- n -，其中R是-(CH 2 -CH 2 -O) m -CH 2 -CH 2 -；x是2～6的整数；n是10～400的整数；并且m是2～5的整数，以及使用该橡胶组合物制备的轮胎。

By rational designs of mechanical structure and plan, the system uses PLC as the core of control on site, and accomplishes control of the installment and debug of the system step by step by software configuring design of the touch panel. Industrial computer, the centre of monitor, control and management of the system, composes epigyny and hypogyny machines' real-time monitoring and controlling system with PLC by designing the communicative model and program, and accomplishes man-machine dialogue in form of suppositional panel by designing supervisory software. Meanwhile, we also adopt client/server model and realize long-distance supervision of impact sensitivity experiment by program designing.

系统通过合理的机械结构和方案设计，采用PLC作为现场控制核心，通过对触摸屏软件组态设计，完成了系统现场安装、调试时的单步控制功能；作为系统的监控、调度中心的工控微机通过通讯模式和程序设计，与PLC组成上、下位机实时监控系统，间接地实现了对撞击感度试验的监控，并通过上位机实时监控管理软件的设计，以虚拟面板的形式较好地实现了人机对话；采用客户机／服务器模式，通过软件编程实现了撞击感度试验的远程监控功能。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

The battle once begun, its very various changes,--the resistance of Hougomont; the tenacity of La Haie-Sainte; the killing of Bauduin; the disabling of Foy; the unexpected wall against which Soye's brigade was shattered; Guilleminot's fatal heedlessness when he had neither petard nor powder sacks; the miring of the batteries; the fifteen unescorted pieces overwhelmed in a hollow way by Uxbridge; the small effect of the bombs falling in the English lines, and there embedding themselves in the rain-soaked soil, and only succeeding in producing volcanoes of mud, so that the canister was turned into a splash; the uselessness of Pire's demonstration on Braine-l'Alleud; all that cavalry, fifteen squadrons, almost exterminated; the right wing of the English badly alarmed, the left wing badly cut into; Ney's strange mistake in massing, instead of echelonning the four divisions of the first corps; men delivered over to grape-shot, arranged in ranks twenty-seven deep and with a frontage of two hundred; the frightful holes made in these masses by the cannon-balls; attacking columns disorganized; the side-battery suddenly unmasked on their flank; Bourgeois, Donzelot, and Durutte compromised; Quiot repulsed; Lieutenant Vieux, that Hercules graduated at the Polytechnic School, wounded at the moment when he was beating in with an axe the door of La Haie-Sainte under the downright fire of the English barricade which barred the angle of the road from Genappe to Brussels; Marcognet's division caught between the infantry and the cavalry, shot down at the very muzzle of the guns amid the grain by Best and Pack, put to the sword by Ponsonby; his battery of seven pieces spiked; the Prince of Saxe-Weimar holding and guarding, in spite of the Comte d'Erlon, both Frischemont and Smohain; the flag of the 105th taken, the flag of the 45th captured; that black Prussian hussar stopped by runners of the flying column of three hundred light cavalry on the scout between Wavre and Plancenoit; the alarming things that had been said by prisoners; Grouchy's delay; fifteen hundred men killed in the orchard of Hougomont in less than an hour; eighteen hundred men overthrown in a still shorter time about La Haie-Sainte,--all these stormy incidents passing like the clouds of battle before Napoleon, had hardly troubled his gaze and had not overshadowed that face of imperial certainty.

交锋以后，战争的非常复杂惊险的变化，乌古蒙的阻力，圣拉埃的顽抗，博丹的阵亡，富瓦战斗能力的丧失，使索亚旅部受到创伤的那道意外的墙，无弹无药的吉埃米诺的那种见死不退的顽强，炮队的陷入泥淖，被阿克斯布里吉击溃在一条凹路里的那十五尊无人护卫的炮，炸弹落入英军防线效果不大，土被雨水浸透了，炸弹陷入，只能喷出一些泥土，以致开花弹全变成了烂泥泡，比雷在布兰拉勒出击无功，十五营骑兵几乎全部覆没，英军右翼应战的镇静，左翼防守的周密，内伊不把第一军的四师人散开，反把他们聚拢的那种奇怪的误会，每排二百人，前后连接二十七排，许多那样的队形齐头并进去和开花弹对抗，炮弹对那些密集队伍的骇人的射击，失去连络的先锋队，从侧面进攻的炮队突然受到拦腰的袭击，布尔热瓦、东泽洛和迪吕特被围困，吉奥被击退，来自综合工科学校的大力士维安中尉，冒着英军防守热纳普到布鲁塞尔那条路转角处的炮火，在抡起板斧去砍圣拉埃大门时受了伤，马科涅师被困在步兵和骑兵的夹击中，在麦田里受到了贝司特和派克的劈面射击和庞森比的砍斫，他炮队的七尊炮的火眼全被钉塞，戴尔隆伯爵夺不下萨克森－魏玛亲王防守的弗里谢蒙和斯莫安，第一○五联队的军旗被夺，第四十五联队的军旗被夺，那个普鲁士黑轻骑军士被三百名在瓦弗和普朗尚努瓦一带策应的狙击队所获，那俘虏所说的种种悚听的危言，格鲁希的迟迟不来，一下便倒在圣拉埃周围的那一千八百人，比在乌古蒙果园中不到一个钟头便被杀尽的那一千五百人死得更快，凡此种种迅雷疾风似的意外，有如阵阵战云，在拿破仑的眼前掠过，几乎不曾扰乱他的视线，他那副极度自信的龙颜，绝不因这些变幻而稍露忧色。

After three years working experience, we has achieved many design projects successfully includes, the second stage landscape engineering design of Huankou Garden developed by Wuhan Real Estate Investment Group, the planning design and architecture design of Zonglian International Residential District developed by Wuhan Zonglian investment Co., Ltd, Phoenix Town developed by Wuhan Huayi Real Estate Co., ltd, Chutian Thermal Spring Hotel developed by Liannin Jingjiang Tourism company, Enshi Genevese Amorous District, the landscape design for new office building of Wuhuan Tech joint-stock co., ltd, landscape design for headquarter of Zhongye Southern Engineering Technical Co., Ltd, office and technical research building of Zhongtie 11 bureau, landscape design for the Xiangshan Minyuan developed by Yichang Hongxiang group Co., Ltd, planning for the Tingtao Guanhai district developed by Wuhan Hongyu Industry Co., Ltd and etc. all of the projects above got the good estimate form our clients.

公司成立三年以来，出色地完成了多项设计工作，其中包括武汉地产开发投资集团的汉口花园二期景观工程设计、武汉众联投资有限公司的众联国际小区的规划、建筑设计、武汉市华盈房地产有限公司的凤凰城、咸宁锦江旅业公司的楚天温泉假日大酒店、恩施日内瓦风情小区、五环科技股份有限公司新办公大楼景观设计、中冶南方工程技术有限公司总部景观设计、中铁十一局集团有限公司办公科研楼、宜昌弘洋集团公司的香山铭苑景观设计、武汉宏宇实业有限公司的听涛观海的规划、建筑与景观设计等项目，深受各开发商和业主的好评。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

According to the studied results at present, the studied objects are mainly thin plate and beam about sound radiation mode, a method computed sound radiation mode of complex structure is presented in this dissertation, according to the results of the previous two chapter, a theoretical method to solve acoustical radiation mode and acoustical radiation efficiency of complex structure by boundary element method and acoustical radiation theory is posed, the acoustical radiation power is expressed as a Hermitian quadratic form, while the radiation modes is determined by general eigenvalue decomposing, and then the radiation efficiency is computed via the orthodoxy of radiation modes with regard to impedance matrix and average velocity matrix; lastly, the validity of the method is proved by pulsating sphere and radiating cube with analytical results. According to results of the form three chapter, the structure sound radiation sensitivity is studied in detail, the sound radiation power of structure can be expressed as positive Hermitian quadratic form, the sound radiation sensitivity can be expressed as two parts by partial differential with respect to design variable, which are sensitivity of boundary velocity and impedance matrix, combined with the theory of FEM and BEM, the structure sound radiation can be translated to the analysis of structure dynamic sensitivity and impedance matrix sensitivity. The theory posted in this dissertation is tested by the FEM software ANSYS and the software AAS programmed by author.

在前几章的基础上，通过结构声辐射的模态理论对结构的声辐射的机理进行了深入地探讨，针对目前声辐射模态的研究对象主要是简单的板和梁类结构，提出了一种计算复杂结构声辐射模态的方法，利用前两章研究所得的结论，将边界元方法与广义特征值的理论结合起来研究了复杂结构的声辐射模态与声辐射效率，先将结构的声辐射功率表示为一个正定的厄米特二(来源：ABb7C论文0909网www.abclunwen.com)次型，运用广义特征值分解求解了复杂结构的声辐射模态，然后利用声辐射模态关于阻抗矩阵与均方速度耦合矩阵的正交性，求解了复杂结构的声辐射效率，最后用具有解析解的脉动球与辐射立方体验证了该方法的有效性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。