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By using two-stage separations indicates that, the content of total ketones compound by one stage separation is 12.14%, far lower than 54.95% by two-stage separations, and the content of pulegone and menthone increases from 3.37% and 3.46% by one-stage separation to 19.34% and 22.95% by two-stage separations respectively. But the content of the long chain unsaturated fatty acid and its ester ingredient reduces from 41.58% and 18.88% by one stage separation method to 29.06% and 9.86% by two-stage separations method respectively, also the phenol compound by two-stage separations method can been separated completely.

对荆芥挥发油采用两级分离的研究表明：酮类化合物的总含量由二级分离的54.95%远高于一级分离的12.14%，其中胡薄荷酮和薄荷酮的含量分别由一级分离的3.37%和3.46%增加到二级分离的19.34%和22.95%，而长链不饱和脂肪酸及其酯类成分含量分别从一级分离法的41.58%和18.88%降低到二级分离法的29.06%和9.86%，且二级分离法已完全除去酚类化合物。

The thesis hold the viewpoints as follows: Dong zhong-shu inherited the ideology of Confucianist School in pre-Qin days by adopting the thought of "Grand Unification","Policy of Benevolence" and "Neutral Political Point", absorbed the ideology of Taoist School by accepting the thought of "Rule by Nonaction" in the relationship of monarch-ministers and monarch-people, incorporated the ideology of Mohist School by embracing the thought of "Heaven"s Will" and "Unify to One", assimilated the ideology of Legalist School by drawing the thought of "Being Dominated by Morals with the Assistance of Penalty" and "Unify to Laws, merged the ideology of Yin-Yang School by assuming the thought of Yin-Yang and the Five Elements.

本文认为：在&大一统&论、德治论、&中和&论等方面，董仲舒继承了先秦儒家的&定于一&、&施仁政&、&致中和&等思想；在君臣关系、君民关系等方面，董仲舒吸收了道家的&无为而治&思想；在&天志&论、&大一统&论等方面，董仲舒兼容了墨家的&天志&、&上同于一&等思想；在&德主刑辅&论、&大一统&论等方面，董仲舒汲取了法家的&德刑并用&、&天下统一于法&等思想；在&天&的范畴、君主独尊地位的合理性、&尊德卑刑&论、&三统说&等方面，董仲舒融合了阴阳家的阴阳五行学说。

The CFb in blank control group was added with IMDM containing 10% FBS and those in Ang Ⅱcontrol group with Ang Ⅱ.The CFb in the 3 test groups were induced with Ang II,then added with Tau at dosages of 40,80 and 160 mmol/L respectively. Determine the proliferation of CFb by MTT method, the collagen content by oxyproline kit, the transforming growth factor- TGF-β1 (TGF TGF-β1) content by ELISA, the cell cycle by flow cytometer, the CFb NO content by nitrate reductase method, the iNOS activity by spectrophotometry, and the expression of iNOS protein by IFA.

胰酶消化法分离培养新生大鼠CFb，用Ang Ⅱ诱导促进其增殖，采用MTT法检测细胞增殖；羟脯氨酸试剂盒检测胶原含量；ELISA法测定转化生长因子β1(TGF-β1)蛋白的含量；流式细胞仪检测细胞周期；硝酸还原酶法、分光光度法和免疫荧光法检测CFb NO含量、一氧化氮合酶活性和iNOS蛋白的表达。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Personality traits were evaluated by Eysency Personality Questionnaire that was compiled by Eysency and revised by Gong. Standard scores of Psychoticism, Extraversion and Neuroticism were calculated, and taking 50 points as a boundary. Each dimension was assigned into 2 grades non-psychotic type (P0) psychotic type (P≥50), introversion type (E0), extraversion type (E0), non-nervous type (N0) and nervous type (N≥50). 5 mL ulnar vein blood was obtained without adding decoagulant, and then serum was removed. Genome DNA was extracted from blood clot by conventional chloroform for PCR analysis. PCR product was sequenced by pyrophosphoric acid. SNP was determined by PSQ96 MA sequenator. Allele frequency was analyzed by mass spectrometry.

人格评定采用Eysency编制，龚耀先修订的艾森克人格问卷、计算精神质，内外问，神经质3个个性维度的标准分，并以50分为界，将各维度分为两个等级：非精神病倾向型（精神质0）、精神病倾向型（精神质≥50）；内倾型（内外向0），外倾型（内外向≥多50）；非常经质倾向型（神经质0），神经质倾向型（神经质≥50）取肘静脉血5 mL，不加抗凝剂，弃血清，血凝块常规酚氯仿提取基因组DNA，进行PCR分析PCR产物应用焦磷酸测序PSQ96 MA测序仪进行SNP检测，用质谱法进行及等位方基因频率分析。

All those whom God has predestinated to life, and those only, he is pleased, in his appointed and accepted time, effectually to call, Ro 8:30 11:7 Eph 1:10,11 by his word and Spirit, 2Th 2:13,14 2Co 3:3,6 out of that state of sin and death in which they are by nature, to grace and salvation by Jesus Christ; Ro 8:2 Eph 2:1-5 2Ti 1:9,10 enlightening their minds spiritually and savingly to understand the things of God; Ac 26:18 1Co 2:10,12 Eph 1:17,18 taking away their heart of stone, and giving to them an heart of flesh; Eze 36:26 renewing their wills, and by his almighty power determining them to that which is good; Eze 11:19 Php 2:13 De 30:6 Eze 36:27 and effectually drawing them to Jesus Christ; Eph 1:19 Joh 6:44,45 yet so as they come most freely, being made willing by his grace. So 1:4 Ps 110:3 Joh 6:37 Ro 6:16-18 2 This effectual call is of God's free and special grace alone, not from any thing at all foreseen in man; 2Ti 1:9 Tit 3:4,5 Eph 2:4,5,8,9 Ro 9:11 who is altogether passive in it, until, being made alive and renewed by the Holy Spirit, 1Co 2:14 Ro 8:7 Eph 2:5 he is enabled by it to answer this call, and to embrace the grace offered and conveyed in it.

2Jo 1：9-11 1Co 16：22 Ga 1：6-8 1 ）所有的人对上帝有predestinated人生观，以及那些只，他很高兴，在他的任命，并接受了时间，有效地打电话，滚装8：30 11时07分弗1：10，11由他的文字和精神， 2th 2：13，14 2co 3：3，6出该国的罪恶和死亡的来信，他们都是本质上说，以恩典和救赎，由耶稣基督；滚装8：2弗2：1-5 2ti 1：9，10启发他们的头脑，在精神上和savingly了解事情的上帝；交流电26：18 1co 2：10，12弗1：17，18拿走他们铁石心肠，并给他们一个心脏的果肉；新教徒协会36：26自强不息他们的遗嘱，以自己的全能的能力决定他们说，这是个好；新教徒协会11时19分的PHP 2时13德30：6新教徒协会36：27 ，并有效地借鉴他们耶稣基督；弗1：19 joh 6：44，45然而，让他们来最自由，正在取得愿意由他的恩典，所以1：4的PS 110：3 joh 6时37滚装6：16-18 2 ）本显效呼吁是上帝的自由和特别恩典单，而不是由任何事情在所有能预见在男子； 2ti 1时09山雀3：4，5厄2：4，5，8，9滚装9时11分，他们是完全处于被动，直到，正活着，并再次由圣灵1co 2时14滚装8时07弗2时05分，他是启用的，它要回答这个号召，并接受恩典提供，并转达了此事。

The experimental results indicated that the addition of yellow serofluid had better nitrogen source effects than carbamide with its optimal addition level as 2 %(cell concentration increased by 18 %, bud ratio increased by 16 %, and liquor yield by alcohol fermentation increased by 2.2 %), which could settle the problem of environment pollution induced by direct discharge. The use of yellow serofluid in the production of alcohol from cassava could increase alcohol content in fermenting solution and liquor yield by 14.2 %Vol and by 35.5 % respectively.

结果表明，添加黄浆水作为酒母培养的氮源效果优于尿素；最适添加量为2 %；成熟酒母发酵液中细胞浓度比尿素作营养盐时提高18 %，出芽率提高16 %，酒精发酵出酒率提高2.2 %；可解决直接排放带来的环境污染问题；用于木薯原料酒精发酵，发酵液中酒精度可达14.2 %Vol，原料出酒率为35.5 %。

Water samples were extracted and purified and collected by solid-phase extraction LC-18 column. Soil samples were extracted by ethyl acetate/petroleum ether (2:1, by vol) and purified by sulphonation. Wheat sample was extracted by acetonitrile/water (4:1, by vol) and purified and collected by SPE- Florisil column.

水样直接用LC-18固相萃取小柱分离、净化和富集；采用乙酸乙酯／石油醚（体积比2：1）振荡提取，磺化法净化，测定土壤中氯氟吡氧乙酸残留量；以乙腈／水（体积比4：1）为提取剂，氟罗里硅土固相萃取小柱分离、净化，测定小麦样品中氯氟吡氧乙酸的残留量。

By a man's finger-nails by his coat-sleeve by his boots by his trouser-knees by the callosities of his forefinger and thumb by his expression by his shirt-cuff - By each of these things a man's calling is plainly revealed.

一个人的手指甲、衣袖、靴子和裤子的膝盖部分，大拇指与食指之间的茧子、表、衬衣袖口等等，不论从以上所说的哪一点，都能明白地显露出他的职业来。

By a man's finger-nails,by his coat-sleeve,by his boots,by his trouser-knees,by the callosities of his forefinger and thumb,by his expression_r_r_r,by his shirt-cuffs,by each of these things a man's calling is plainly revealed.

一个人的手指甲、衣袖、靴子和裤子的膝盖部分，大拇指与食指之间的茧子、表情、衬衣袖口等等，不论从以上所说的哪一点，都能明白地显露出他的职业来。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。