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Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

Not to inherit by right of primogeniture, gavelkind or borough English, or possess in perpetuity an extensive demesne of a sufficient number of acres, roods and perches, statute land measure (valuation 42 pounds), of grazing turbary surrounding a baronial hall with gatelodge and carriage drive nor, on the other hand, a terracehouse or semidetached villa, described as Rus in Urbe or Qui si sana , but to purchase by private treaty in fee simple a thatched bungalowshaped 2 storey dwellinghouse of southerly aspect, surmounted by vane and lightning conductor, connected with the earth, with porch covered by parasitic plants, halldoor, olive green, with smart carriage finish and neat doorbrasses, stucco front with gilt tracery at eaves and gable, rising, if possible, upon a gentle eminence with agreeable prospect from balcony with stone pillar parapet over unoccupied and unoccupyable interjacent pastures and standing in 5 or 6 acres of its own ground, at such a distance from the nearest public thoroughfare as to render its houselights visible at night above and through a quickset hornbeam hedge of topiary cutting, situate at a given point not less than 1 statute mile from the periphery of the metropolis, within a time limit of not more than 15 minutes from tram or train line (e.g., Dundrum, south, or Sutton, north, both localities equally reported by trial to resemble the terrestrial poles in being favourable climates for phthisical subjects), the premises to be held under feefarm grant, lease 999 years, the messuage to consist of 1 drawingroom with baywindow (2 lancets), thermometer affixed, 1 sittingroom, 4 bedrooms, 2 servants' rooms, tiled kitchen with close range and scullery, lounge hall fitted with linen wallpresses, fumed oak sectional bookcase containing the Encyclopaedia Britannica and New Century Dictionary, transverse obsolete medieval and oriental weapons, dinner gong, alabaster lamp, bowl pendant, vulcanite automatic telephone receiver with adjacent directory, handtufted Axminster carpet with cream ground and trellis border, loo table with pillar and claw legs, hearth with massive firebrasses and ormolu mantel chronometer clock, guaranteed timekeeper with cathedral chime, barometer with hygrographic chart, comfortable lounge settees and corner fitments, upholstered in ruby plush with good springing and sunk centre, three banner Japanese screen and cuspidors (club style, rich winecoloured leather, gloss renewable with a minimum of labour by use of linseed oil and vinegar) and pyramidically prismatic central chandelier lustre, bentwood perch with fingertame parrot, embossed mural paper at 10/- per dozen with transverse swags of carmine floral design and top crown frieze, staircase, three continuous flights at successive right angles, of varnished cleargrained oak, treads and risers, newel, balusters and handrail, with steppedup panel dado, dressed with camphorated wax: bathroom, hot and cold supply, reclining and shower: water closet on mezzanine provided with opaque singlepane oblong window, tipup seat, bracket lamp, brass tierod and brace, armrests, footstool and artistic oleograph on inner face of door: ditto, plain: servants' apartments with separate sanitary and hygienic necessaries for cook, general and betweenmaid salary, rising by biennial unearned increments of 2 pounds, with comprehensive fidelity insurance, annual bonus (1 pound and retiring allowance (based on the 65 system) after 30 years' service, pantry, buttery, larder, refrigerator, outoffices, coal and wood cellarage with winebin for distinguished guests, if entertained to dinner, carbon monoxide gas supply throughout.

505他并不想根据长子继承制、男子平分继承制或末子继承制，把那幢有着门房和马车道的男爵宅邪及其周围那一大片辽阔的英亩、路得和平方杆法定土地面积单位，（估价为四十二英镑的泥炭质牧场地，或者那座被描述为&都会中的田园&或&健康庄&的有阳台的房子或一侧与邻屋相接的别墅，继承下来并永久占有。他只巴望根据私人合同购买一所继承人身分不受限制的不动产：要坐北朝南的一座草屋顶、有凉台的双层住宅，房顶上装起风向标以及与地面相接的避雷针，门廊上要爬满寄生植物，橄榄绿色的正门最后一道工序漆得漂漂亮亮，赛得过马车。门上有着精巧的黄铜装饰。房屋正面是灰泥墁的，屋檐和山墙涂着金色网眼花纹。尽可能让房子耸立在坡度不大的高台上，从那圈着石柱栏杆的阳台上，隔着现在空着、将来也不得占用的牧场地，可以聎望四周的一片好景致。单是自己的庭园，就有五、六英亩之谱。它与最近的公路的距离适度：夜晚从修剪得整整齐齐的鹅耳枥树篱上端和缝隙间，可以瞥见室内的灯光，从首都边界的任何地点丈量，与这所房子相距至少也有法定一英里。不出十五分钟就可以轰电车或火车铁道沿线。（例如往南去登德鲁姆或往北去萨顿，就像是南北两极。经过验证，据说这两处气候都适合肺结核患者。）凭着继承人身分不受限制的不动产转让证拥有房屋和地基，租借期限为九百九十九年。宅邸里包括一间有着凸窗的客厅，一间起居室，四间卧室，两间仆役室。砌了瓷砖的厨房里还安装了多用途的铁灶和洗涤台，休息厅里备有放亚麻布床单衬衫用的壁橱，分成几层的氨熏橡木书柜，放着《大英百科全书》和《新世纪辞典），横陈着一把把中世纪或东洋的古老刀剑；还有通知开饭的锣，雪花石膏做的灯，悬垂着的饰钵，附有电话号码簿的胶木自动电话听筒；手织的阿克斯明斯特地毯，是奶油色质地，周围镶着棋盘图案。有着兽爪形柱脚的牌桌。壁炉装着大型黄铜格栅，炉台上摆着精密的镀金计时表，准确无误地发出大教堂那样的钟声，附有湿度计的晴雨表，蒙着鲜红色长毛绒面子、装着上等弹簧、中心部位富于弹性的舒适的长靠椅和放在角落里的备用椅，日本式三扇屏风，痰盂（俱乐部里摆的那种，用深紫红色皮革制成，只要用亚麻子油和醋一擦，不费吹灰之力就能发出光泽，焕然一新。）室中央悬挂一盏金字塔式枝形吊灯，射出灿烂的光辉。一截弯木上栖着一只驯顺得能停在手指上的鹦鹉，墙上糊着每打价为十先令的压花壁纸，印着胭脂红色垂花横纹图案，顶端是带状装饰；一连三段栎木楼梯，接连两次拐成直角，都用清漆涂出清晰的木纹，梯级、登板、起柱、栏杆和扶手，一律用护板来加固并涂上含樟脑的蜡；浴室里有冷热水管，盆汤、淋浴，设备俱全。位于平台上的厕所里，长方形窗子上嵌着一块毛玻璃，带盖的坐式抽水马桶，壁灯，黄铜拉链和把手，两侧各放着凭肘几和脚凳，门内侧还挂有艺术气息浓厚的油画式石版画。另外还有一间普通的厕所；厨师、打杂的女仆和兼做些细活的女佣的下房里也分别装有保健卫生设备（仆役的工钱每两年递增两英镑，并根据一般忠诚勤劳保险，每年年底发奖金一英镑，对工龄满三十年者，按照六十五岁退职的规定，发退职金）；餐具室、配膳室、食品库、冷藏库、主楼外的厨房及贮藏室等、堆煤柴用的地窨子里还有个葡萄酒窖（不起泡、亮光闪闪的葡萄酒），这是为宴请贵宾吃正餐时预备的。对整座楼房都供应一氧化碳瓦斯。

Floral organ determination is best explained by the ABCDE model postulated by genetic studies of Arabidopsis thaliana. Sepals are determined by A and E class genes; petals are determined by A, B, and E; stamens by B, C, and E; and carpels by C and E class genes. A, B, C, and E class gene lineages are known having duplicated several times during the evolution of angiosperms. One of the noted major duplication events occurred in the origin of the early angiosperms, leading to the formation of subgroups of B/C/D/E class. Another one occurred near the basal eudicots and gave rise to further subgroups in A/B/C/D/E class genes among core eudicots. The phylogenetic position of the family Buxaceae is located right where the second major duplication of ABCDE genes might have occurred, which is supported by multiple gene (nuclear 18S rDNA, chloroplast rbcL and atpB) phylogenetic analyses.

目前经由模式植物阿伯芥的研究，建花部器官决定基因的调控，即花萼由A、E 群基因共同决定，花瓣由A、B、E 群基因，雄蕊由B、C、E 群基因，而心皮由C 和E 群基因决定。A、B、C、E 四群基因在被子植物的演化过程中发生过次的复制事件，其中比较重要的一次发生在早期被子植物演化出之时，形成B/C/D/E 群基因的次系群；另一次复制事件则发生在真双子植物基群附近，形成仅於核心真双子植物的A/B/C/D/E 群基因之次系群。

What\'s more, different intervals of UV have their own characters in influencing DNA: the damage to some bases by UVA may be more serious than that by UVB, and UVB did more severe damage to some deoxyribose than UVA; however, as far as the pyrimidine bases are concerned, the damage by UVB is by far more serious than that by UVA. These results may indicate that there are preferential damaging sites on DNA molecules caused by UVA and UVB irradiation. Moreover, with UVA or UVB irradiation for a long time, the photoreactivation of DNA occurred. The serious damage to deoxyribose and bases by UVB and UVC may confirm the conformation of cyclobutane pyrimidine dimers and (6-4) photoproducts.

而不同波段紫外辐射对DNA的损伤各具特点并且差异明显，UVA对一些碱基的损伤比UVB要严重，而UVB在对脱氧核糖和磷酸盐的损伤上又比UVA要严厉，但就嘧啶碱基来说，UVB对它的损伤远大于UVA，这似乎表明了不同的紫外波段对生物大分子DNA的损伤是有选择性的。

Furthermore, the complications detected by spiral CT and chest radiography were as follows: emphysema was detected in 11 cases by CT and six by radiography; pulmonary tuberculosis was detected in five cases by spiral CT and three by radiography; lymph node enlargement or calcification in mediastinum or pulmonary hilus were detected 21 cases by CT and 15 by radiography.

CT检出矽肺并发肺气肿11例，X线胸片检出6例；CT检出矽肺并发肺结核5例，X线胸片检出3例；CT检出矽肺并发纵隔和肺门淋巴结大或钙化21例，X线胸片检出15例。

Furthermore, the complications detected by spiral CT and chest radiography were as follows: emphysema was detected in 11 cases by CT and six by radiography; pulmonary tuberculosis was detected in five cases by spiral CT and three by radiography; lymph node enlargement or calcification in mediastinum or pulmonary hilus were detected 21 cases by CT and 15 by radiography.

CT检出矽肺并发肺气肿11例，X线胸片检出6例；CT检出矽肺并发肺结核5例，X线胸片检出3例；CT检出矽肺并发纵隔和肺门淋巴结或钙化21例，X线胸片检出15例。

By using two-stage separations indicates that, the content of total ketones compound by one stage separation is 12.14%, far lower than 54.95% by two-stage separations, and the content of pulegone and menthone increases from 3.37% and 3.46% by one-stage separation to 19.34% and 22.95% by two-stage separations respectively. But the content of the long chain unsaturated fatty acid and its ester ingredient reduces from 41.58% and 18.88% by one stage separation method to 29.06% and 9.86% by two-stage separations method respectively, also the phenol compound by two-stage separations method can been separated completely.

对荆芥挥发油采用两级分离的研究表明：酮类化合物的总含量由二级分离的54.95%远高于一级分离的12.14%，其中胡薄荷酮和薄荷酮的含量分别由一级分离的3.37%和3.46%增加到二级分离的19.34%和22.95%，而长链不饱和脂肪酸及其酯类成分含量分别从一级分离法的41.58%和18.88%降低到二级分离法的29.06%和9.86%，且二级分离法已完全除去酚类化合物。

The thesis hold the viewpoints as follows: Dong zhong-shu inherited the ideology of Confucianist School in pre-Qin days by adopting the thought of "Grand Unification","Policy of Benevolence" and "Neutral Political Point", absorbed the ideology of Taoist School by accepting the thought of "Rule by Nonaction" in the relationship of monarch-ministers and monarch-people, incorporated the ideology of Mohist School by embracing the thought of "Heaven"s Will" and "Unify to One", assimilated the ideology of Legalist School by drawing the thought of "Being Dominated by Morals with the Assistance of Penalty" and "Unify to Laws, merged the ideology of Yin-Yang School by assuming the thought of Yin-Yang and the Five Elements.

本文认为：在&大一统&论、德治论、&中和&论等方面，董仲舒继承了先秦儒家的&定于一&、&施仁政&、&致中和&等思想；在君臣关系、君民关系等方面，董仲舒吸收了道家的&无为而治&思想；在&天志&论、&大一统&论等方面，董仲舒兼容了墨家的&天志&、&上同于一&等思想；在&德主刑辅&论、&大一统&论等方面，董仲舒汲取了法家的&德刑并用&、&天下统一于法&等思想；在&天&的范畴、君主独尊地位的合理性、&尊德卑刑&论、&三统说&等方面，董仲舒融合了阴阳家的阴阳五行学说。

The CFb in blank control group was added with IMDM containing 10% FBS and those in Ang Ⅱcontrol group with Ang Ⅱ.The CFb in the 3 test groups were induced with Ang II,then added with Tau at dosages of 40,80 and 160 mmol/L respectively. Determine the proliferation of CFb by MTT method, the collagen content by oxyproline kit, the transforming growth factor- TGF-β1 (TGF TGF-β1) content by ELISA, the cell cycle by flow cytometer, the CFb NO content by nitrate reductase method, the iNOS activity by spectrophotometry, and the expression of iNOS protein by IFA.

胰酶消化法分离培养新生大鼠CFb，用Ang Ⅱ诱导促进其增殖，采用MTT法检测细胞增殖；羟脯氨酸试剂盒检测胶原含量；ELISA法测定转化生长因子β1(TGF-β1)蛋白的含量；流式细胞仪检测细胞周期；硝酸还原酶法、分光光度法和免疫荧光法检测CFb NO含量、一氧化氮合酶活性和iNOS蛋白的表达。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。