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In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株(KB-A-1)的多药抗药性问题,本文从反义核酸和反基因核酸角度,通过MTT法检测细胞生长情况,ELISA法检测基因表达产物P-gp表达水平的变化,对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

The little girl is always taken care of by Catharine.

有些动词短语本身即是被动语态的形式,不要再加 by 短语,常用的有

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C. communis grown in normal soil. Several aspects were examined, including: difference of copper tolerance and accumulation between C. communis grown in copper mine deposit and that grown in normal soil; difference of growth and nutrient accumunation between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; physiological differences between between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; subcellular distribution and chemical forms of Cu in C. communis; identification of cDNA fragments differentially expressed in C. communis grown in copper mine deposit; genetic diversity and copper tolerance of C. communis, tolerance mechanisms to copper in C. communis are discussed.

中文题名鸭跖草对铜的耐性机理和富集特征研究副题名外文题名 Mechanisms of Cu tolerance and accumulation by Commelina communis 论文作者廖斌导师蓝崇钰束文圣学科专业环境科学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数128页关键词重金属污染植物修复超富集植物土壤污染铜污染鸭跖草馆藏号BSLW /2003 /X53 /12 论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料,分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异,两种生境鸭跖草在铜胁迫下养分吸收和积累的差异,两种生境鸭跖草在铜胁迫下生理反应的差异,以及铜在鸭跖草细胞内的分布及结合形态研究,矿山鸭跖草差异表达的cDNA克隆,铜对鸭跖草种群遗传分化的影响,从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

I just finished reading The Road by Cormac MacCarthy.

我刚读完The Road by Cormac MacCarthy。

Cv. BY-2 and carrot Daucus carota L.

cv.BY-2和胡萝卜Daucus carota L。

A kind of pellet desulfurization reagent made by pulverized coal ash and slaked lime was filled in the fixed-bed and the simulated moving-bed. The desulphur and dust collection performance of the reactor were studied.

中文题名颗粒床脱硫和除尘的研究副题名外文题名 Studies on desulphurization and dust collection by the granular bed 论文作者黄学敏导师马广大教授学科专业环境工程研究领域\研究方向学位级别博士学位授予单位西安建筑科技大学学位授予日期2001 论文页码总数79页关键词脱硫烟气除尘烟气脱硫颗粒床馆藏号BSLW /2003 /X701 /13 本文研究了以粉煤灰、消石灰和石膏制备的粒状脱硫剂,在固定床和模拟移动床实验装置中对粒状脱硫剂层的脱硫和除尘性能进行了实验。

The results showed that the strain BY-28 couldrapidly grow and reproduce well in fishmeal wastewater, at the same it also synthesizedintracellularly and excreted extracellularly flocculant, which provided technical andeconomic feasibility for further production of microbial flocculant on pilot-scale.

结果表明,菌BY-28在鱼粉废水培养基中能够快速生长繁殖,并生产胞外分泌物即微生物絮凝剂,为进一步开展微生物絮凝剂的中试试验提供了技术经济的可行性。

However, I'm happy enough to be able to stay in the hotel which I always drop by whenever I visit Singapore and it's all because of the Hainanese Chicken Rice… HEHE!!!

不过,我很开心这次住的饭店是之前我每到新加坡都会drop by的饭店,全都因为&海南鸡饭&…嘿嘿!!!

However,I'm happy enough to be able to stay in the hotel which Ialways drop by whenever Ivisit Singapore and it's all because of the Hainanese Chicken Rice…HEHE!

不过,我很开心这次住的饭店是之前我每到新加坡都会drop by的饭店,全都因为&海南鸡饭&…嘿嘿!

However, I'm happy enough to be able to stay in the hotel which I always drop by whenever I visit Singapore and it's all because of the Hainanese Chicken Rice… HEHE!!!

不过,我粉开心这次住的饭店是之前我每到新加坡都会drop by的饭店,全都因为&海南鸡饭&…嘿嘿!!!

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。