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Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

Methods 4-Hydroxy- benzonitrile was treated with sodium hydrogen sulfide and anhydrous magnesium chloride in dimethyl formamide to give thioamide, which was then directly cyclized with ethyl 2-chloroacetoacetate without separation to give ethyl 2-(4-hydroxyphenyl)-4-methylthiazole-5-carboxylate(2) in one-pot; then 2 was formylated with Duff reaction adopting hexamethylenetetramine in trifluoroacetic acid to give ethyl 2-(3-formyl-4-hydroxyphenyl)-4-methylthiazole-5-carboxylate(3); finally, the target compound was obtained by the treatment of 3 with hydroxylamine hydrochloride and sodium formate in formic acid.

采用&一勺烩&方法，以4-羟基苯甲腈为起始原料，首先与硫氢化钠和无水氯化镁在N，N-二甲基甲酰胺中反应，所得中间体不经分离，直接加入2-氯乙酰乙酸乙酯进行环合反应，得到2-(4-羟基)苯基-4-甲基-5-噻唑甲酸乙酯（2）；然后通过六亚甲基四胺/三氟乙酸进行Duff反应，得到2-(3-甲酰基-4-羟基)苯基-4-甲基-5-噻唑甲酸乙酯（3）；再经盐酸羟胺/甲酸/甲酸钠体系脱水得到目标化合物。

Then validate the theory mentioned above by analysis. At last, in order to impel the rural and small town enterprises getting together into town, we should strengthen townish development;perfect the rural land market for nonfarm construct;and then can improve urbanization at last.

最后，进一步提出加强城镇自身建设，增强其经济要素聚集功能，以及建立统一的城乡建设用地市场，完善农村集体非农建设土地流转市场的必要性，以形成乡镇企业向城镇聚集的土地成本推进效应，促进农村城镇化水平提高。

From then on, we then walked, all day playing, I was also surprised inthe father slowly uncommunicatively change by the silence in a livelyway am open and bright.

从那以后，我们俩便走到了一块，整日的玩耍，我也在父亲的惊奇中慢慢的由沉默寡言变得活泼开朗起来。

Sensible of a benignant persistent ache in his footsoles he extended his foot to one side and observed the creases , protuberances and salient points caused by foot pressure in the course of walking repeatedly in several different directions , then , inclined , he disnoded the laceknots , unhooked and loosened the laces , took off each of his two boots for the second time , detached the partially moistened right sock through the fore part of which the nail of his great toe had again effracted , raised his right foot and , having unhooked a purple elastic sock suspender , took off his right sock , placed his unclothed right foot on the margin of the seat of his chair , picked at and gently lacerated the protruding part of the great toenail , raised the part lacerated to his nostrils and inhaled the odour of the quick , then with satisfaction threw away the lacerated unguinal fragment

随后他弯下身去，解起打成结子的靴带：先掰搭钩，松开靴带，再一次一只只地脱下靴子235 。右边那只短袜湿了一部分，大脚趾甲又把前面捅破并伸了出去，这下于便跟靴子分开了。他抬起右脚，摘下紫色的松紧袜带后，扒下右面那只袜子，将赤着的右脚放在椅屉儿上，用手指去撕扯长得挺长的大拇脚趾甲，并轻轻地把它拽掉，还举至到鼻子那儿，嗅嗅自己肉体的气味，然后就心满意足地丢掉从趾甲上扯下来的这一碎片。

For strong convective weather , it classified all historical sample events into 4 weather patterns (like northwest , trough area ,west wind and southwest current), established 4 characteristic fields of 400hPa height of HLAFS , then according to the principle of the pattern match calculate and compare the real-time HLAFS forecast products-2-using the similar method inside the big and small key areas , establish forecast equation , finally gain conclusion .To duststrom weather, the historical samples were sorted into duststrom and severe duststrom types in 5 regions (they are whole area, west, central, middle-west and middle-east of Inner Mongolia ),moreover set up different sample databases about ECMWF fields (including 500hPa height, 850hPatemperature and sea-level pressure).In order to making duststrom forecast at different time level , we first filtered the real-time data by FAX data ,and then used the method of similar range degree to compare the historical data to the actual data of ECMWF .To precipitation weather ,they were divided into 2 types that suit or unsuit airplane artificial precipitation stimulation in line with their emergence time and district .The appropriate weather physical factors come from T106 were chosen to establish the artificial precipitation stimulation prediction model . In the actual application , we can get prediction result as long as use the real-time forecast data of T213 in the prediction model .

针对强对流天气将历史样本天气分为西北气流型、槽区型、西风气流型和西南气流型等4个类型，并建立其HLAFS资料400hPa四种特征场，按照模板匹配的原理，用相似分析方法在大、小两个关键区中对实时HLAFS预报产品进行计算、比较，再建立预报方程，得出结论；对沙尘暴天气将其按区域分为全区、西部、中部、中西部以及中东部5类，再按强弱分别分为强和一般2类，建立ECMWF的3个场(500hPa高度场、850hPa温度场和海平面气压场)的历史资料库，在用传真资料消空之后，用相似离度方法计算实时ECMWF资料，做出不同时次的预报；对降水天气按出现时间和区域分为适合和不适合飞机增雨作业2类，选择T106资料中恰当的气象物理量因子，用BP神经网络算法建立人工增雨降水预报模型，实际应用中将实时T213相关预报资料代入预报模型即可。

This paper first introduces a system which can detect vehicle disobey the red light. then introduces a arithmetic with shadow elimination to solve the problem of violations detection of yellow line: by day,the quality of the image is good,this arthmetic uses a method which combine the line-frame detection and the HSV color information analysis.this method detect violations according as whether the line-frame of yellow is being destroyed. In order to solve the most important problem in apply instance—interference of shadow,we add HSV color information analysis in the area of yellow line which line-frame is being destroyed. If the shadows proportion is below the threshold,then we determine vehicle violative of yellow line; at night,the quality of the image is not good, we calculate the grey image of differences to detect violations.

本算法在白天图片质量较好时，采用结合HSV颜色空间分析线结构检测算法，该算法利用黄线区域的边缘结构特征作为依据检测是否存在车辆或阴影压黄线，针对实际应用中存在的最大问题即阴影的干扰，加入了HSV颜色空间分析来检测黄线边缘残缺区域，若该位置阴影比重低于一定比例，才认定为是车辆压黄线违章而不是阴影干扰；在夜晚图片质量下降的时候，采用灰度差值法。

Since then, he gave up on the studying for a while and entering 'Hapdong Film Company' in order to have experiences with dream to be an actor, he began as the junior staff going through planning department, directing department, and production department, etc., And then, challanging after seeing the public notice on the audition for KBS drama 'Salut d'Amour' directed by Yoon Seokho PD in 1994, as he was cast as the main character, he began to walk a path of full-scale actor.

从此之后，他有一阵子放弃升学，为了要体验演员梦而进入了& Hapdong电影公司&，他以资深工作人员的身份开始，待过企划部、导演部、制作部等等。后来，1994年尹锡瑚执导的KBS连续剧&爱的问候&公开试镜，裴勇俊挑战试镜成为主角之后，他就开始了全方位演员之路。

The dynamic changes of the the neuron apoptosis and correlative genes in hippocampus of ACET-groups and twice-ACET-groups suggested that ACET can slower the apoptosis by inhibiting p~(53)and prolong the ascending period of p~(53) in 4 days after the seizure,this effect would weaken after 4 days.ACET may inhibit the descending of Bax,prolong its descending period and then promote its expression,in order to weaken the inhibition of the apoptosis during 4 to 10 days after seizure and then promote the apoptosis during 10 to 14 days after.This effect of ACET can be enhanced after treating again within 7 to 14 days later.

埋线组和二次埋线组大鼠海马神经元凋亡及相关基因表达的动态变化结果提示：埋线治疗在癫痫发作后的早期(4d内)可通过抑制促凋亡基因p~(53)蛋白表达、延缓其达峰值的时程，从而减缓神经元凋亡的发生，此作用在4d后减弱；埋线治疗抑制大鼠海马神经元凋亡的作用在癫痫发作后的中期(4d～10d)减弱，甚至在远期(10d～14d)出现促进凋亡的作用，可能是通过抑制Bax蛋白表达的下降、延长其降至低峰值的时程，促进Bax蛋白表达的回升的结果；在中远期(7d～14d)内予二次埋线治疗对该效应有续效叠加的作用。

The reaction of the grumpiest, most graceless man in British public life was to lambast the press for celebrating Beckham's achievement and then drop him for the next United game, and to keep dropping him over the next three months of a season in which the team ended up winning nothing - in large measure because of the confusion Ferguson had sown among the team by saying he was quitting, and then changing his mind; and possibly owing also to the energy and passion he redirected at his highly successful horse, Rock of Gibraltar.

对于失败，福格森由于宣布辞职然后变卦在球队中引起的困惑以及他把大量精力投入了赛马的行为应负主要责任。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。