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Methods: In accordance with conventional reporter gene analysis reagent preparation method, the main component Luciferase, Luciferin and ATP were serial diluted to find the optimal concentration; Reaction solution was prepared according to the optimal concentration of the main component, then ATP were serial diluted to measure their reporter gene. Then standard curve of ATP were drawed. Tumor cell lines H1299 were serial diluted and their ATP were measured by reaction solution prepared to analyse the minimum cell number.

按照常规报告基因分析试剂配制方法，将其中主要成分Luciferase和Luciferin及ATP浓度进行倍比稀释后找到最适合反应的最佳浓度值；按照最佳浓度配制反应液，将ATP浓度倍比稀释测定报告基因数值并绘制标准曲线，分析检测灵敏度；另外选取肿瘤细胞株H1299，倍比稀释后裂解细胞应用配制好的反应液进行ATP检测，分析最低检测细胞数。

It is the first time in my life to earn a lot of money, then we would like to go home a little time gift to you, if I said to you, then you are not in favor of me to the so-called waste, Mum, I have grown up, and not let you by fear of no longer allow you to me and maleficence dream.

是我人生第一次挣的这么多钱，当时我们就想回家的时间带点礼物给您，如果我和您说的话，您是不会赞成我去所谓的浪费，妈，我长大了，再不会让你担心受怕了再也不会让您为我而做恶梦了。

Then we apply it to a C-module V, where V is a n-dimensional vector space and the operator from C×V to V is defined by a linear transformation T of V, then we get a unique factorization of V and a right basis under which the transformation matrix of T is T's Jordan canonical form.

然后把它应用到一个具体的C-模V，其中V是n维线性空间，C×V到V的映射由V中的一个线性变换T定义，从而得到V的一个唯一分解，再结合线性代数有关知识给出V的一组基，T在这组基下的变换矩阵恰为T的Jordan标准形。

I then smoothed some of the noise out by running it through a low setting median filter , and then reset the white point as the median had reduced the contrast too much.

然后我平滑的一些噪音是由运行它通过一个低设置中值滤波，然后重置白点为中位数减少了反差太大。

We will then define the type of actions of which comedy is the mimesis, then we will examine the means by which comedy excites laughter, and these means are actions and speech.

我们将为喜剧所摹仿的行为下定义，然后讨论喜剧是以何种方式引人发笑的。这些方式包括行为与语言。

Based on some solvent recovery technology process, chemical miscellany performance has been discussed in this paper with the modeling method meanwhile. Then, the paper proposes a new controlling model method by using Time Petri Net to the miscellany process, which is also a start-up process. Then, the simulation software platform is developed.

本文以某化工溶剂回收的开停车过程为背景，分析了化工开停车过程这个混杂系统的行为特性和建模方法，在此基础上，研究了基于赋时Petri网的开停车过程系统建模与控制方法，并开发出相应的仿真平台。

Then it was past and the land flattened out, emptying of houses and roads, of human endeavour and misdemeanour, to be replaced by scrubland, occasional swamp, and then warthog, zebra and impala running from the train.

之后，是一片平地，房子和公路，人类的劳作和滋事，在之后是灌木丛，偶尔是沼泽地，再看到的是疣猪，斑马和黑斑羚从火车面前奔跑。

The representative minority cultural relic-pottery relic of Buyi minority and the two wooden masks is taken as the research object. Firstly, Hiscanner laser scan detector was adapted for data collection, and then obtain point cloud data which could be recorded on disc for permanent saving was obtianed; Furthermore, the new popular reverse engineering software Geomagic for some disposals of point cloud data was adopted in data condensing, data mending, data filtering, feature line picking up to obtain NURBS surfaces; and then the error between the reconstructed CAD model and the point cloud data was evaluated to guarantee the precision of surface reconstruction to keep the relics keep its original appearance. In the end, the NURBS surfaces are imported to 3D software Solidworks and entities of CAD are generated by surface sewing, mold loft and ablating and other instructions.

选用有代表性的贵州少数民族文物―布依族酒壶、水壶以及木制傩戏面具作为研究对象，采用Hiscanner激光扫描测头对其进行数据采集，扫描得到的点云数据可刻录到光盘上作为文物数据永久保存；更进一步地，采用目前流行的逆向工程软件Geomagic studio对点云数据进行数据精简、数据滤波、数据修补、提取特征线等处理后得到NURBS曲面；为保证曲面模型重建的精度，在曲面重建模型后对其误差评价，通过控制误差的方式指导曲面模型重建，使重建的文物曲面模型能真实完整地反映原状；最后，在功能强大的三维造型软件Solidworks中导人重构的NURBS曲面进行曲面缝合、放样等，将其生成实体CAD模型，并对模型的局部特征进行适当的再设计以实现文物实体模型的优化。

Then, in 1830, Edwin Beard Budding, an engineer from Gloucestershire, came up with a third alternative—"a machine for mowing lawns, etc." Supposedly, Budding was inspired by the rotating blades then used to trim the nap on carpets.

到了1830年，格罗斯特郡的一个工程师艾德温·比尔德·巴登带来了第三种选择――"一种割草的机器"（根据推测，巴登是从修剪地毯上杂毛的旋转刀刃上得来的灵感）。

Free. If you were holding a Mudra, Jie Yin, then you release the Mudra, San Yin, by raising the Mudra to your forehead, and then separate your hands.

自由。如果你正在结印，那麼你就散印，举印至你的额头，然后分开双手。问：《密乘初阶》第三十三页，「将诸尊汇入本尊而修之」是指什麼？

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。