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Citri had been selected by ribosome display in previous research. In order to express the scFvs with bio-activity efficiently, the genes of the selected scFvs were transformed into Escherichia coli HB2151 for expressing resoluble antibodies. The expression levels of scFvs were detected by dot blotting and Western blotting, and the expressed scFvs were purified by affinity chromatography, and then the activity of the purified scFvs was tested by enzyme-linked immunosorbent assay .

Citri， Xac高亲和力单链抗体(Single chain variable fragment， scFv)GX13、GX44和GX95，为高效表达具有生物活性的单链抗体，本研究将前期筛选的高亲和力单链抗体基因转入大肠杆菌HB2151中进行可溶性表达，点印迹和Western印迹检测可溶性单链抗体的表达水平，亲和层析纯化表达的单链抗体，酶联免疫吸附法检测纯化单链抗体的抗原结合活性。

In this paper, as a case of Karamay, we have studied on space-time changement of soil quality in oasis of arid area. We measured quality for different land use types and years in same type by combinativing field sampling for Karamay in Xinjiang and analysis in laboratory, which is directed by former theory of soil quality. Then we did integrated evaluation of soil quality for different land use types, and had drew some conclusions as followed: In four land use types, the soil bulk density of layers(0～15cm) for farmland and tree land is bigger than the other land use types.The organic matter of every layer in manpower Tamarix Bulrush land is bigger; organic matter of Tamarix Bulrush land release efficiency nitrogen which result ration of C/N is higher; total nitrogen of all layers in farmland variety great as being effected by mass use of nitrogen in agriculture; deadwood and defoliation of tree、shrub and bulrush pooled in 5cm layer to result that quick nitrogen is the biggest among all layers. The total salt of nature shrub tree land for every layer being bigger than farmland explain that nature shrub thee land strongly stand salt and farmland for basification in this area; that the nutrient of face layer is bigger than the ground layer may be explained by that vegetation gather nutrient to be stored in deadwood and defoliation.

本文对以克拉玛依为例，对干旱区绿洲土壤质量的时间空间变化进行研究，在有关土壤质量的研究理论指导下，通过田间调查取样和室内分析相结合的方法，对新疆克拉玛依市农业开发区的不同土地利用方式及同一土地利用方式下不同利用年限土样土壤理化性质进行测定，从而对新垦绿洲不同土地利用类型土壤质量进行综合评价，结果如下：四种土地利用方式中，农地、乔木林地表层(0-15cm)土壤容重较大；各土层人工柽柳芦苇地的土壤有机质含量均较大；柽柳芦苇地的有机质在矿化过程中所释放出有效氮，导致柽柳芦苇地的C/N比例较高；受到农业耕作使用大量氮肥的影响，农地各土层全氮含量变异较明显；乔木、灌木及芦苇的枯枝落叶主要集中在5cm范围之内，导致几种土地利用方式类型下该层土壤速效氮最大；天然灌木林地的土壤总盐在各层均较大，其次是农地，说明天然灌木林地的高耐盐能力，以及该区农业生产用地的土壤盐碱化程度较重。

During building 2D model, I use the concepts of point, segment, line and plane, the segment can be formed from points, then line can be formed from segments, the closed curved plane can be obtained by around lines, and finally the 2D model can be built by closed curved plane; I express the un-smooth stratum or fault by linear fitting and express the smooth stratum or default by cubic spline fitting segmentally, in this way I can describe the variation feature of 2D interface properly.

在二维建模中，引用点、段、线、面的概念，即由点形成段，由段形成线，由线围成封闭面，封闭面构建成二维模型；对表示二维地层和断层的界面线，用线性拟合表示非光滑性地层或断层面，用分段三次样条拟合表示光滑性地层或断层面，恰当地表达了二维界面的变化特点。

Firstly, the waste sodium was formed into sodium oxide by burning, and the sodium oxide residue was treated by atomized water. Then, the residue was formed into solution by water flow. Finally, it was neutralized by acid.

首先，将废钠燃烧生成钠的氧化物；再用水雾初步处理钠的燃烧产物；然后，用水喷淋的方法将全部燃烧产物转化成水溶液；最后，用酸中和成中性溶液。

According to structural characteristics of floating slab tracks, a track segment element was taken between two adjacent fasteners. For each element, rails were regarded as Euler beams supported by discrete viscoelastic supports. The fasteners and rubber supports were replaced by a linear spring and damp. So the vibration model of the floating slab track was established. In constructing vibration model of a metro train, each car of the metro train with two suspensions was modeled as a multi-rigid body system, in which rigid bodies were connected with each other by a linear spring and damp. Combining the potential energy of vertical vibration of the track with that of the metro train, the total potential energy of vertical vibration of the train and track was obtained. And then, the matrix equation of vertical vibration of the system was established using the principle of total potential energy with stationary value in elastic system dynamics and the "set-in-right-position" rule for formulating system matrices. The vibration responses of the system can be obtained by solving the matrix equation with the direct time integration such as Wilson-θ method.

摘 要：针对浮置板式轨道结构特点，取相邻2个扣件之间的轨道为1个轨段单元，钢轨视为连续弹性点支承Euler梁，浮置板视为弹性薄板，扣件系统及橡胶支座均模拟为线性弹簧及粘滞阻尼器，建立浮置板式轨道振动模型；将城轨列车中的车辆均离散为多刚体系统，各刚体之间通过线性弹簧及粘滞阻尼器相连，建立列车振动模型；将浮置板式轨道及列车振动势能叠加，得到系统竖向振动总势能；基于弹性系统动力学总势能不变值原理及形成系统矩阵的"对号入座"法则，建立此系统竖向振动矩阵方程；采用Wilson-θ逐步积分法求解此矩阵方程，得出此系统竖向振动响应。

METHODS: CNE-2 human nasopharyngeal carcinoma cells were exposed repeatedly to γ-rays and then screened for a new radioresistant subline named CNE-2R. Double times of CNE-2 and CNE-2R cells were determined by MTT assays. The relative radiosensitivities of tumor cells were assessed by standard colony formation assays. Survival rates after exposed to irradiation were measured by MTT assays. The time courses of cell cycle distribution before and after irradiation were investigated by flow cytometry.

用γ射线反复照射CNE-2细胞，筛选出放射抗拒细胞株CNE-2R；检测细胞生长的倍增时间；应用细胞克隆形成实验检测细胞的放射敏感性；MTT法检测照射后不同时间的存活分数；用流式细胞术检测细胞的周期分布特征。

In the process of laboratory study, oxycodone was prepared by oxidating thebaine at 6,14-position under oxydol, hydrodeoxidizing 7,8-position double bond and 14-position ether under palladium catalyst. Furthermore, N-methyl of oxycodone was isolated byα-chloro-ethyl chloroformate under anhydrous condition and protection of 14-position hydroxyl to obtain noroxycodone, which was further converted into methylnaltrexone by N-alkylation in presence of cyclopropyl bromomethane, then by isolating 3-position methyl of methylnaltrexone under boron tribromide, becoming hydrochloride to prepare target compound, natrexone hydrochloride.

实验室工艺方面，本文首先用双氧水氧化蒂巴因的6-位和14-位、在钯炭催化下加氢还原7-8位双键和14-位醚合成羟考酮，然后提出并优化了在无水和14-位保护的情况下，用氯甲酸-α-氯乙酯脱去羟考酮的N-位甲基合成去甲羟考酮，再用环丙甲基溴将去甲羟考酮的N-位烷基化合成甲基纳曲酮，最后采用三溴化硼脱去甲基纳曲酮的3-位甲基和成盐酸盐合成盐酸纳曲酮。

Methods The antimicrobial peptides of housefly larvae were induced largely by infection and injury, which were isolated and purified by trituration, centrifugalization and column chromatography. Then the antimicrobial peptides with anti-Toxoplasma activity were sieved by methyl thiazolyl tetrazolium colorimetric method and haemacytometry. The DNA contents of T.gondii tachyzoites were detected by flow cytometry.

通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，分离纯化并筛选出抗弓形虫作用的抗菌肽，采用流式细胞术分析其对弓形虫速殖子DNA含量的影响。

HPLC-MS data should be supplemented to the HPLC fingerprint information for a secondary peak matching.BP artificial neural network is then constructed by MATLAB 5.3 to recognize the fingerprints of Epimedium Brevicornum by the pattern recognition model based on the fingerprints of TCM. The model discriminates 5 categories of crude Epimedium Brevicornum from each other successfully.Inspired by the success of blended wine, attempts are made for the first time to simulate the trueborn crude Epimedium Brevicornum by blending the crude Epimedium Brevicornum of various sources.

针对指纹图谱相似度评价的不足，初步探讨了BP人工神经网络在中药指纹图谱模式识别中的应用；结果表明，基于matlab5.3编程建立的BP网络模式识别模型能将5个类别的淫羊藿药材准确区分开来，预测率满意，可以作为相似度评价的补充；同时进行了网络结构的考察，确定了隐含层最佳节点数。

The articles published by Xiuhua News Agency quotes some of the means summarized by experts, which are adopted by America to manipulate the exchange rate: through some deceiving investment reports or some research outcomes to release misleading information into the market; oversold Euros and sterling pounds by hedge funds through hedge funds recently, and may attack Yen in the near future; presses RMB to appreciate to the level he expects through political, diplomatic and trading means; undersold Euros through stabilization fund to raise the value of dollar and lower the value of Euro, and then printed his home currency at a large scale to offset its bad debts.

新华社上述文章引用了专家归纳的美国操纵汇率的一些手法：通过带有欺骗性的投行报告、专家研究结果等，向市场释放诱导信息；近期通过对冲基金沽空欧元、英镑等外币，未来还有可能攻击日元；耍弄政治、外交和贸易手段，压迫人民币按其要求和标准升值；在金融危机中，先是通过平准基金大量抛售欧元，导致欧元狂跌、美元上涨，然后大肆印制钞票，填平坏账。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新