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Methods: Mesityl oxide in the air was sampled by active carbon tube and desorbed by the solvent of carbon disulfide which included 1% methanol, then separated by HP-5 capillary column,and finally detected by mass detector.

工作场所空气用活性炭管采集，经1%甲醇的二硫化碳溶液解吸，HP-5毛细色谱柱分离，气质联用仪检测。

The expressed product was purified by affinity chromatography and identified by Western blot, then determined for enzyme activity by Micrococcus lysodeikticus turbidimetric method and for biological activity by heat shock method.

表达产物经亲和层析纯化后，进行Western blot鉴定，并用溶壁微球菌比浊法检测酶活性，热激法检测其生物学活性。

Fifty female patients who were selected to schedule for mammary gland tumor excision surgery, were divided into 2 groups(n=25 in each group): group A: received propofol's half-effective concentration at 3.0 μg/ml in Marsh model by TCI; group B: received propofol's half-effective concentration at 4.0 μg/ml in Marsh model by TCI. All patients were given remifentanil in Minto model by TCI after being given propofol which reached the balance between effect-site concentration and blood plasma concentration. The target concentration of remifentanil was determined by a modification of Dixon's up-and-down method. Laryngeal mask insertion was attempted after 10 mins, the insertion condition and success rate were observed, then the suitable target concentration of remifentanil(ECe50) were calculated.

50例女性乳腺良性手术患者随机分为2组（n=25），A组：靶控输注丙泊酚效应室浓度3.0 μg/ml；B组：靶控输注丙泊酚效应室浓度4.0 μg/ml（均为Marsh药代模型），待其效应室浓度和血浆浓度达平衡后给予靶控输注效应室浓度瑞芬太尼，运用Dixon's序贯法确定喉罩置入时瑞芬太尼浓度，10分钟后置入喉罩并观察喉罩置入条件与成功率，计算出相应的瑞芬太尼适宜靶浓度（ECe50值）。

METHODS: A total of 1 536 people in rural areas of shaanxi were studied by cluster random sampling methods. All particants were checked by slit lamp, and the people who were more than 50 years old carried out the standard examination by internal criteria. Then they were examined by LOCS Ⅲ in mydriatic situation when there were no contraindication.

遵循按比例、随机、整群抽样的原则对陕西省洋县 1 536人进行白内障患病率的横断面调查，所有受检人员均采用裂隙灯检查晶状体，年龄≥50岁的人，先在小瞳孔下按照国内诊断标准进行检查，如无禁忌再充分散瞳后，按LOCSⅢ标准进行检查。

The death rate grew by 37%. Chinese previously did not need security door. But by now, to some place, security window has been set at seventh floor. For there are too many gangster every where, no company in China would arrange with their workwomen to go home at night. No body knows what really are the food every one have everyday. Neoteny has take place on children. Then we can imagine the situation of our health in the future. There are about 20 million girls in China are pursuing sexual work. That creates 6% of GDP aggregate, which is as much as 1000 billion RMB. This kind of activity had never appeared in the history of human being except Japan. The height of Chinese people is 2.5cm lower than that of Japanese. We can no longer see them by looking down. The small Japan became big Japan. We create 0.1 billions GDP with the payment of 1 person's life, reported by reference news. The number of death in 2003 is 136 thousands. So the number of industrial injury will be 200 thousands. It is true that our GDP is bloody. Actually, this number is a small part of the true number. The numbers which can be accounted are in nationalized business or in a big accident. In personalized business or overseas-funded enterprise, the owner do not pay attention on the death of workers. So the data can not pass to the statistical department. However, the number of death there is much more than that of the first ones. If we add this in account, the number,annually can be compared with the massacre in Nanjing.

中国人从原来不知道防盗门窗为何物，到现在防盗窗已安装到了楼房的七层以上；由于流氓遍地防不胜防，全国企业早已不再安排女工上下夜班；有毒食品已经100%的覆盖了全部行业，每天人们咽进肚子里的食物究竟是什么，只有天知晓；性早熟现象已经蔓延到了学龄前儿童，未来的身体和寿命可想而知；约2000万少女被迫卖淫，创造的收入占GDP总量的6%，相当于一万多亿人民币，这种肉体积累是世界历史上除日本之外绝无仅有的现象；中国人的平均身高比日本人低了2.5公分，由以前低头看日本人到现在抬头看日本人，小日本真的成了"大日本"；《参考消息》报道，中国每亿元GDP工伤死亡1人，2003年死亡达13.6万人，以此推算，今年工伤死亡人数将达到20万，"是名副其实的带血GDP"，其实这个死亡数字不过是冰山一角，能够统计到的死亡数字，要么是国有企业，要么是死人较多的特大事故，私企和外企平常死个把人根本到不了统计部门，而私企和外企用工数量远远超过国有企业，如果考虑到这个因素，每年死亡人数至少相当于一场南京大屠杀。

First by testing the practical multilayer insulation in a cryogenic tanker, which is the carilometer in my paper,some affecting factors on multilayer insulation are analysed, then by using the low-temperature and vacuum environment caused by multilayer insulation, the effective thermal conductivity of multiple fiber paper with 5 layers is tested.Through Newton interativemethod,an empirical formula is deduced to computer the thermal conductivity of fiber paper,The errors between experimental thermal conductivity and the calculated conductivity by empirical formula are below10%,so the formula of fiber conductivity can be applied to practical enginnering.

为探索改善工程应用中高真空多层绝热层绝热性能的方法，本文实验研究中首先测量了包扎在实际低温储罐上不同结构的高真空多层绝热层的绝热性能，并分析了环境温度、环境压力，层数、不同隔热材料的组合等因素对工程应用中的高真空多层材料绝热性能的影响；然后利用多层绝热层中的低温真空环境测量5层干法纸在不同温度区间内的表观导热系数，在有限实验数据的基础上用牛顿迭代数值拟和出计算5层干法纸表观导热系数的经验公式，对比经验公式计算值和实验测量值，发现二者的误差在10%以内，因此在工程应用中可用本文的经验公式计算干法纸在低温下的导热系数。

First, the proposed scheme uses contourlet transform to extract multidirectional and multiscale texture information, and then obtains the feature points invariant to the affine transformation from the low and middle directional subbands by Harris-Affine detector. Second, the feature regions are adaptively computed by the feature scale of the local structure and normalized by U transform. The watermarking is embedded adaptively in those characteristic regions which are normalized. Finally, by vector quantization, several copies of the watermark are embedded into the no overlapped local feature regions in different directional subbands.

首先，用Contourlet变换提取出多尺度、多方向的纹理信息；再用Harris-Affine检测算子从变换域的中、低频方向子带中提取出仿射不变特征点，结合自适应局部结构的特征尺度确定特征区域，并用U变换对其归一化处理，水印就自适应地嵌入到归一化后的区域中；水印嵌入采纳矢量量化的策略，将水印信息重复嵌入到不同方向子带、多个不相交的局部仿射不变特征区域。

Therefore, with the approval of the sacred Lateran council, by apostolic authority and fullness of power, we approve and renew, and order to be observed and maintained in their totality and without change, the said letter together with each and every statute, ordinance, decree, explanation, agreement, compact, promise, wish, penalty, restraint and clause contained in it; especially the clause by which it was our will that if the said king of France does not approve and ratify the aforesaid letter, and each and every thing contained in it, within six months from the date of this present letter, and does not arrange for the contents to be read, published, sworn to and registered, like all other royal constitutions in his kingdom and in all other places and lordships of the said kingdom, for all future time without limit, by all the prelates and other ecclesiastical persons and courts of parlements, and if he does not convey to us, within the said six months, letters patent or authentic written documents concerning each and all of the aforesaid matters about the acceptance, reading, publication, oath and registration referred to, or does not deliver them to our nuncio attached to the king, in order to be passed on by him to us, and does not subsequently arrange for the letter to be read each year and effectively observed without alteration exactly as other binding constitutions and ordinances of the king of France have to be observed, then the letter itself and whatever follows from it are null and void and of no force or value .

因此，在徵得安理会的神圣拉特兰，由使徒的权威和充实力量，我们赞成和更新，并以得到遵守和维护他们的整体，没有变化，该信连同每一个章程，条例，法令解释，协议，契约，承诺，希望，罚款，限制和条款，载於它；特别是其中的条款，这是我们的意志，如果说法国国王不同意和批准上述信件，和每一个事载於它，在6个月内之日起的本信，并没有安排的内容来阅读，出版，发誓要和登记，象所有其他王国的宪法，他在英国和其他所有的地方和lordships英国的说，今后所有的时间没有限制，所有的主教和其他教会人员和法院parlements ，如果他不转达给我们，在上述6个月，英皇制诰或真实的书面文件和所有的每一个上述事项的接受，阅读，出版，宣誓和注册提到，或者没有交给我们的大使重视国王，以便通过他给我们，并没有随后安排信每年读和有效地观察到完全没有改变的其他具有约束力的宪法和法令的法国国王都必须遵守，那麼，这封信本身，无论从它是无效的，没有武力或价值。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

Finally this thesis analyses the efficient revocable group signature scheme with forward security proposed by Chen Shaozhen and Li Daxirig. An adversary can personate a legal group member by forge a group membership certificate, and then can forge group signatures that can be verified by a verifier. And the group manager can also forge group signatures that can be verified by a verifier. So the scheme is insecure. Meanwhile, there are redundancy steps in the scheme, thus the efficiency is low.

接着，对陈少真和李大兴提出的一种有效取消的前向安全群签名方案进行了密码学分析，一方面，攻击者可以通过伪造群成员证书来伪造能够通过验证的群签名；同时，群管理员也可以伪造一个可以通过验证的群签名，因此，该群签名体制是不安全的；另一方面，该体制存在冗余数据。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新