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Full length LMP2A cDNA was firstly incised from pGEM-T-LMP2A with EcoR Ⅰ, Sma Ⅰ digestion, and then inserted into eucaryote. expression plasmid pCIcc controlled by CMV promoter. The CMV-LMP2A-SV40 expression unit was digested by ClaI, and inserted into E1-substituted adenovirus vector pAx1cw. Then the LMP2A recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I digested Ad5-TPC.

将带有LMP2A cDNA的重组质粒pGEM-T-LMP2A用EcoR Ⅰ、Sma Ⅰ双酶切下LMP2A cDNA，并将其插入含同样酶切位点的真核表达质粒pCIcc中，使其受控于CMV启动子下；用ClaI切下CMV-LMP2A-SV40表达单元，插入E1、E3区替代的腺病毒载体pAX1CW，选择正确的克隆pAX1CW-LMP2A与Ad5 DNA-末端肽复合体共转染293细胞，通过同源重组获得复制缺陷型的重组腺病毒(Ad5-LMP2A)。

To help improve Henan Netcom Company's ability of competition, we also make the environmental analysis, the strategy analysis and the competitive analysis; distinct it's external internal strength as well as weakness opportunity, threat. We assure that the core competition abilities include advanced telecommunication network, large numbers of subscribers and various services. Then this text analysis the competitors, the potential competitors, substitutions, equipment providers and customers by using Porter theory, compare each competitor's ability and the field that they compete drastically. Through conforming the costumer's utilitarian and personal services requirement, it discover that the key factor of development is holding the group subscribers. By Boston Matrix we find out key services, staring services, problem services, then study which service is the most important for us and which one should be throw off. Base on analysis above, this article concludes the general strategy and the service strategy of Henan Netcom: enforce the stable development strategy for certain period, and perform expansion strategy when time is fit.

在对河南省通信公司的发展战略的分析中，以战略管理理论战略分析为基础，进行了河南省通信公司的内部的优势和劣势以及外部机遇和威胁分析，确定了河南网通公司的优势是先进的通信网络、庞大的客户资源和丰富的业务；运用波特五种竞争力分析方法，通过竞争对手分析、潜在竞争对手分析、替代品分析、供应商分析、用户分析，探讨了几个竞争者实力对比和相互竞争激烈的领域；明确了用户需求是在方便和实用的同时，追求个性化服务，得出了今后竞争的重点是保住关键的大客户；运用波士顿矩阵分析法，找出了河南省通信公司的金牛业务、明星业务和问题业务，研究了公司各种业务产品的发展重点和取舍战略。

I scarce knew in what Manner to receive them; but found to draw our selves in a close Line was the only Way: so we form'd in a Moment: But that we might not have too much Interval, I order'd, that only every other Man should fire, and that the others who had not fir'd should stand ready to give them a second Volley immediately, if they continued to advance upon us, and that then those who had fir'd at first, should not pretend to load their Fusees again, but stand ready with every one a Pistol; for we were all arm'd with a Fusee, and a Pair of Pistols each Man; so we were by this Method able to fire six Volleys, half of us at a Time; however, at present we had no Necessity; for upon firing the first Volley, the Enemy made a full Stop, being terrify'd as well with the Noise, as with the Fire; four of them being shot into the Head, dropp'd, several others were wounded, and went bleeding off, as we could see by the Snow: I found they stopp'd, but did not immediately retreat; whereupon remembring that I had been told, that the fiercest Creatures were terrify'd at the Voice of a Man, I caus'd all our Company to hollow as loud as we could; and I found the Notion not altogether mistaken; for upon our Shout, they began to retire, and turn about; then I order'd a second Volley to be fir'd, in their Rear, which put them to the Gallop, and away they went to the Woods.

我简直不知道如何对付它们。结果，我认为最好的办法是我们互相靠拢，排成一行。于是，我们马上照此行事。为了不致使我们的火力中断太久，我下令只许一半人开枪，另一半人作好准备；如果第一排枪响过后，狼群继续向我们冲来，就开第二排枪；同时，在开第二排枪时，那开第一排枪的一半人，不要忙于装他们的长枪，而是应该抽出手枪，作好准备。因为我们每人身上都有一支长枪和两支手枪。用这种办法，我们可以连续开六排枪，每次有一半人开枪。然而，当时还没有必要这样做。放出第一排枪之后，我们的敌人就给枪声和火光吓坏了，马上停止了前进。有四条狼被我们打中头部，倒了下来；另外有几条受了伤，鲜血淋淋地跑掉了。这在雪地上可以看得一清二楚。我发现，狼群停止了攻击，但没有后退。这时，我忽然记起有人说过，就是最凶猛的野兽，听见人的声音也会害怕。于是我就叫大家拼命呐喊。这个办法果然很有效。我们一喊，狼群就开始后退，掉头跑掉了。我又下令朝它们背后开了一排枪。这样一来，它们才撒腿跑回树林里去了。

I scarce knew in what Manner to receive them; but found to draw our selves in a close Line was the only Way: so we form'd in a Moment: But that we might not have too much Interval, I order'd, that only every other Man should fire, and that the others who had not fir'd should stand ready to give them a second Volley immediately, if they continued to advance upon us, and that then those who had fir'd at first, should not pretend to load their Fusees again, but stand ready with every one a Pistol; for we were all arm'd with a Fusee, and a Pair of Pistols each Man; so we were by this Method able to fire six Volleys, half of us at a Time; however, at present we had no Necessity; for upon firing the first Volley, the Enemy made a full Stop, being terrify'd as well with the Noise, as with the Fire; four of them being shot into the Head, dropp'd, several others were wounded, and went bleeding off, as we could see by the Snow: I found they stopp'd, but did not immediately retreat; whereupon remembring that I had been told, that the fiercest Creatures were terrify'd at the Voice of a Man, I caus'd all our Company to hollow as loud as we could; and I found the Notion not altogether mistaken; for upon our Shout, they began to retire, and turn about; then I order'd a second Volley to be fir'd, in their Rear, which put them to the Gallop, and away they went to the Woods.

放出第一排枪之后，我们的敌人就给枪声和火光吓坏了，马上停止了前进。有四条狼被我们打中头部，倒了下来；另外有几条受了伤，鲜血淋淋地跑掉了。这在雪地上可以看得一清二楚。我发现，狼群停止了攻击，但没有后退。这时，我忽然记起有人说过，就是最凶猛的野兽，听见人的声音也会害怕。于是我就叫大家拼命呐喊。这个办法果然很有效。我们一喊，狼群就开始后退，掉头跑掉了。我又下令朝它们背后开了一排枪。这样一来，它们才撒腿跑回树林里去了。

Adding transition metals into the TiN films by arc ion plating (1) The hardnesses of theN films are higher that that of the TiN films, proving that the alloy hardening effect is a common phenomenon;(2) The structures of the alloyed films are determined by the added element properties such as covalent radius, electro negativity, free energy and lattice parameters of the MN compound;(3) theN film consists of a mixture of multi phases with high hardness but quite brittle, while the other MN films are composed of single phase with the fcc TiN structure and their hardesses, functions of the added amounts of the elements, manifest always a maximum at a certain M percentage.

一、将过渡金属M用电弧离子镀方法添加于TiN薄膜中：1。N薄膜硬度均高于TiN薄膜硬度，合金强化效应普遍存在；2。薄膜相结构决定于添加元素M的周期律信息，包括M的共价半径、电负性，及MN化合物的自由能、晶格常数等；3。N为多相混合结构，薄膜硬度高，但韧性差；其它N的相结构为TiN类型的单一fcc结构，薄膜硬度随M添加量变化并出现峰值；4。

On my way up north up on the ventura i pulled back the hood and i was talking to you and i knew then it would be a life long thing but i didn't know that we we could break a silver lining and i'm so sad like a good book i can't put this day back a sorta fairytale with you a sorta fairytale with you a sorta fairytale with you things you said that day up on the 101 the girl had come undone i tried to downplay it with a bet about us you said that- you'd take it as long as i could i could not erase it and i ride along side and i rode along side you then and i rode along side till you lost me there in the open road and i rode along side till the honey spread itself so thin for me to break your bread for me to take your word i had to steal it and i'm so sad like a good book i can't put this day back a sorta fairytale with you a sorta fairytale with you i could pick back up whenever i feel down new mexico way something about the open road i knew that he was looking for some indian blood and find a little in you find a little in me we may be on this road but we're just imposters in this country you know so we go along and we said we'd fake it feel better with oliver stone till i almost smacked him seemed right that night and i don't know what takes hold out there in the desert cold these guys think they must try and just get over on us and i was ridin' by ridin' along side for a while till you lost me and i was ridin' by ridin' along till you lost me till you lost me in the rear view you lost me i said way up north i took my day all in all was a pretty nice day and i put the hood right back where you could taste heaven perfectly feel out the summer breeze didn't know when we'd be back and i, i don't didn't think we'd end up like like this

在途中提高北方在 ventura 上提高我向后地拉头巾而且我正在和你说话而且我知道然后它会是生活长的事物但是我没有知道那我们我们可以休息一个一线希望而且我很这么忧愁同类一本善行书我不能放这日子背面一近似美丽宛如在神仙故事中的与你一近似美丽宛如在神仙故事中的与你一近似美丽宛如在神仙故事中的与你你说那个日子的事物在 101 上提高女孩有来未完成的我试验过的不予重视它藉由一个打赌有关我们你说那-你将会拿它只要我可以我无法消除它而且我乘坐向前边而且我向前骑边你然后而且我向前骑边直到你失去的我在那里在开着的道路中而且我向前骑边直到蜂蜜传布它本身如此瘦的因为我对休息你的面包因为我拿你所说的话我必须偷它而且我很这么忧愁同类一本善行书我不能放这日子背面一近似美丽宛如在神仙故事中的与你一近似美丽宛如在神仙故事中的与你我可以精选向后地向上的每当我感觉？？落新的 mexico 方法关于开着的道路某事我知道他是找寻一些 indian 血和稍微在你里面稍微找发现在我里面我们可能是在这一条道路上除了我们只是 imposters 在这国家中你知道因此我们向前去和我们说我们有假货它以脚踏铁槌石头感觉好很多直到我几乎有味道他似乎是权利哪一夜晚和我不知道什么控制向那边在那沙漠寒冷这些人认为他们一定尝试而且仅仅在我们身上克服而且我是 ridin'被 ridin'向前边一阵子直到你失去的我而且我是 ridin'被 ridin'向前直到你失去的我直到你失去的我在那后面视野你失去的我我说方法向上的北方我花了我的日子极其重要的是一相当好的日子和我放头巾正确地背面哪里你可以味道天堂完全地感觉夏天微风没有知道当我们将会回来而且我我做不没有想我们有结束在同类上面同类这

The gun was a good time, after sitting a little bigger, a powerful and easy operation, suitable for all kinds of maps environment M16:The gun power, after sitting a little high, and be convenient operation, but more is still poor and m4a1 some ; AK-47:The gun after sitting too large, the most powerful, it is difficult to operate, but to all kinds of maps of the environment is a very good of the gun ; AK－74:Power of the gun, fire is quickly, then take a great improvement of the 74 than 47 more easy to operate FAMAS:The power of a gun, fire, take off after a person closer to the king G36K:The power of a gun, fire is quickly, then sat a small operation is very easy overhand SG552:The gun power, and sat down and large, but the fire, is a good fast screen, sniper rifles AUG:The gun power, after sitting a little fire, and is sure of the higher is the world famous one of the sniper rifle ; AWM:The gun after a powerful, by too big, fast and slow to be very high, who is a widespread use of the sniper rifle ; M700:The gun power larger and smaller, a relatively rapid speed and must have very high, use less M60:The gun power, and sat down and large, the fire, is not high, is a suppressed fire MP5:The gun after power, by a fire made, and must have a high, is a very good lead to a submachine gun ; MP7:The gun after power, and sat down, fire is quickly, he is too poor to cooperate with the team to use

此枪精准高，后坐较小，威力较大，容易操作，适合各种地图环境使用； M16：此枪威力较大，后坐较小，精准较高，方便操作，但是和M4A1比较还是差了点； AK-47：此枪后坐太大，威力更大，难以操作，但是适应各种地图环境是一把十分不错的高杀伤力枪； AK－74：此枪威力较大，射速较快，后坐比较大，改进后的74比47更容易操作； FAMAS：此枪威力较小，射速飞快，后坐较大，人称近距离步枪之王； G36K：此枪威力较小，射速较快，后坐很小，操作非常方便容易上手； SG552：此枪威力较大，后坐也大，但是射速很，是一把不错的快掩护型狙击步枪； AUG：此枪威力较大，后坐较小，射速一般，精准较高，是世界有名的狙击步枪之一； AWM：此枪威力巨大，后坐太大，射速较慢，精准很高，谁一把广泛使用的狙击步枪； M700：此枪威力较大，后作较小，射速较快，精准很高，一般使用人少； M60：此枪威力较大，后坐也大，射速一般，精准不高，是一把压制火力的重机枪 MP5：此枪威力一般，后坐较小，射速飞快，精准较高，是一把非常不错的冲锋与反冲锋枪； MP7：此枪威力一般，后坐较大，射速极快，精准太差，主要适合团队配合才可以使用

This paper introduces the background knowledge of heterogeneous data integration, as well as the knowledge of data integration. On the basis of the above, The author develops an ontology-based Heterogeneous data Integration prototype system, expounds the main framework and essiontial technology. Then the paper introduces its work in detail: it explains the very significance of using ontology as an comon semantic description and an global query model ,and illustrates the reason fo adopting LAV method ; then it builds the formal representation of the mapping between ontology and data resources, applies OWL to describe the ontology and the mapping ; defines a query language named by DIQL to query on our integration system , and supply the detailed arithmetic of reolving partial binding of LAV method、locating of data sources and query rewriting,etc. Finally,the paper demostrates and proves the result of research by examples .

本文介绍了目前异构数据集成的研究现状以及数据集成和本体的相关背景知识，并在此基础上设计开发了一个基于本体的异构数据LAV集成原型系统，同时对原型系统的总体框架、关键实现技术分别做了讨论：首先阐述了将本体作为公共语义描述和全局查询模型的意义，给出了采用LAV集成方案的理由，然后规定了本体和数据源间映射的形式化表示方式，采用能结合描述逻辑和框架系统的优点、适合于半结构化环境的OWL表示本体和映射；其次定义了一种针对集成系统的查询语言DIQL，并在此基础上给出解决LAV部分绑定问题、数据源定位以及查询重写的算法；最后在原型系统的基础上，通过实例验证本文所提方法的可行性。

Touch Yi:Armpit department store big of blood vessel, nerve, lymphoid knot, such as others by hand touch, drive touch then would cachinnation, health care in Shanghai massage is been called "armpit flow" by the expert, husband and wife line this flow, then add 啰 Suo more can line.

触腋：腋窝部贮藏着丰厚的血管、神经、淋巴结，如他人用手触摸，被触者便会大笑，被专家称为&腋窝流动&，夫妻间行此流动，则愈加啰嗦可行。

Italians make dumplings by pressing paste into a strip and then putting stuffing on the strip spoon by spoon. And then they wet the edge of the strip. Next they use the same size the paste to add up to the paste with stuffings ready. Finally they cut the paste into pieces.

他们包铰子是把面压成一长条、一勺勺放好馅，在面的边缘沾上水，再用同样的一条面片合在一起压好，然后用刀一一切开。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新