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Yun thoghts,from yueyingIn dark and hardship,I and my sworb was surrounded by bloody.I who just dispatched troops to fight was surrounded by two monsters.My body was rended by their sharp teech,my blood burested forth.I tried my best to raise my sworb and irritated the monster's hearted abruptly.Then,I didn't have any power to brandish my sworb. My blood running off and my consciousness was disppearing.In a twin kling,I daw a sword sliced the other monster's head,then I fainted.

黑夜，风高。血腥笼罩着提着长剑的我，才出道的我被两个怪物围攻，我身上的血肉被他们锋利的牙齿撕裂，鲜血飞溅出来，我拼尽全力提起手中的长剑，咬咬牙，狠狠地刺穿一只怪物的心脏，此时的我，已经没有任何力气挥动那柄沉重的长剑，我的血液在流淌，我的意识在消亡，在我昏倒的一瞬间，我看见一柄长剑从后面削掉了那怪物的脑袋。

Then pMD-P80 is digested by the enzymes Xho I and Apa I, separated and linked to the linear plasmid pEGPF-C1 after digested by Xho I and Apa I. So the recombined expression plasmid pGFP-P80 is achieved. Then it is sequenced, PCR and digested by the restriction enzymes Xho I and Apa I. The results give that pGFP-P80 is achieved successfully and the insert sites, direction and reading frame are all right. It builds the basis for expressing, purifying, gaining p80 protein from mammiferous cells.

将pMD-P80 分别经Xho I 和Apa I 双酶切和回收，然后与经过Xho I 和Apa I 酶解的真核表达载体pEGPF-C1 连接、转化，获得重组质粒，经PCR，Xho I 和Apa I 限制性酶切和序列测定，鉴定为真核表达质粒pEGFP-P80，并且目的基因的插入位置、方向和读码框完全正确，为在哺乳动物细胞中表达并分离纯化p80 蛋白奠定了基础。

The researchers can get the relative information quickly by collecting, organizing and managing the data and then use the information expediently. We can gain the deep-seated biological knowledge by dealing with and digging the data and then enhance our understanding to the biology world. In the applications and researches of biology and iatrology we can improve the efficiency and scientize the researches and exploitation by using the data and analysed results. Just like to examine the related genes of some disease basing on the result of analysing the genes' fuctions and to design a new medicine basing on the results of analysing the protein.

通过收集、组织、管理生物分子数据，使研究人员能够迅速地获得和方便地使用相关信息；通过处理、分析、挖掘生物分子数据，得到深层次的生物学知识，加深对生物世界的认识；在生物学、医学的研究和应用中，利用生物分子数据及其分析结果，可以大大提高研究和开发的科学性及效率，如根据基因功能分析结果来检测与疾病相关的基因，根据蛋白质分析结果进行新药设计。

Here Pilgrims roam, that stray'd so farr to seek In Golgotha him dead, who lives in Heav'n; And they who to be sure of Paradise Dying put on the weeds of Dominic, Or in Franciscan think to pass disguis'd; [ 480 ] They pass the Planets seven, and pass the fixt, And that Crystalline Sphear whose ballance weighs The Trepidation talkt, and that first mov'd; And now Saint Peter at Heav'ns Wicket seems To wait them with his Keys, and now at foot [ 485 ] Of Heav'ns ascent they lift thir Feet, when loe A violent cross wind from either Coast Blows them transverse ten thousand Leagues awry Into the devious Air; then might ye see Cowles, Hoods and Habits with thir wearers tost [ 490 ] And flutterd into Raggs, then Reliques, Beads, Indulgences, Dispenses, Pardons, Bulls, The sport of Winds: all these upwhirld aloft Fly o're the backside of the World farr off Into a Limbo large and broad, since calld [ 495 ] The Paradise of Fools, to few unknown Long after, now unpeopl'd, and untrod; All this dark Globe the Fiend found as he pass'd, And long he wanderd, till at last a gleame Of dawning light turnd thither-ward in haste [ 500 ] His travell'd steps; farr distant he descries Ascending by degrees magnificent Up to the wall of Heaven a Structure high, At top whereof, but farr more rich appeer'd The work as of a Kingly Palace Gate [ 505 ] With Frontispice of Diamond and Gold Imbellisht, thick with sparkling orient Gemmes The Portal shon, inimitable on Earth By Model, or by shading Pencil drawn.

此外还有很多，说起来未免太冗长，他们无非是些未成熟的、痴呆的、穿着黑、白、灰色衣的身带骗人的假法宝的隐士和托钵僧。其中有的在这里云游巡礼，曾到各各他去寻觅那活在天上的死人，临终穿上圣多明我派或圣方济派的袈裟，以证明自己确实去过极乐天堂，他们误以为这样打扮就可畅通无阻。他们通过七星天，通过&恒星天&，通过那权衡黄道振动均势的&水晶天&，并且还通过&原动天&。圣彼得手拿钥匙，站在天堂的边门口，像是在等待他们，正要举步登上天堂的阶梯时，看吧！从左右吹来一阵猛烈的横风，他们被斜吹到十万里外的远空中去：那时看见僧帽、头巾、袈裟，连同它们的穿戴者一起被吹翻扯烂，还有圣骨、念珠、免罪券、特免证、赦罪证、训谕，全被高高卷起，都成了风的玩具，他们飘过这世界的背面而远落在广大的地狱边缘，被称&愚人的乐园&，很久之后将是家喻户晓的，但是现在却是人迹罕至的地方。魔王走过这整个黑球，经过长久的漫游，终于发现一线曙光，便加紧他的步伐，向那边走去。他远远望见一座高大的建筑，从它那宽大的阶梯拾级而上，可以抵达天国的城垣，它的顶上，像有比王宫还要富丽的宫门，它的正面镶有金刚石和黄金；大门上密饰着东方的珠宝，光彩夺目，人间的浓淡画笔也无法描绘它。

In this paper Ⅰ successfully applied these methods to fault diagnosis, and presenting some new innovative machinery fault diagnosis method; First Ⅰ present a new gear wornout fault diagnosis method, this method use the vibration signal's time-frequency attribute and instantaneous phase undulation to accurately detect gear's fault, and by comparing with the traditional frequency domain analysis method, this method reveals that many nonlinear and nonstationary characteristic exist in gear's vibration, this tell us that if we use frequency domain analysis method without pay attention to the signal's characteristic, we could get wrong diagnostic result; Then Ⅰ present a new bearing fault diagnosis method, this method use ATVFD and Hilbert transform to do envelop demodulation,Ⅰ analyze the envelop with time-frequency method and find out that this method is more accurate then traditional method.Ⅰ also explain that the modulation of the fault to the carrier wave may also be non-stationary and nonlinear; Finally Ⅰ present a cylinder pressure measurement method of diesel engine by means of vibration, cylinder pressure is an important parameter of diesel engine fault diagnosis, the pressure measuring is simplified a lot by this method,Ⅰ also use the ATVFD and time-frequency analysis to realize it.

本文中将上述理论方法成功地应用到故障诊断中去，提出了几点创新性的诊断方法；提出了一种齿轮磨损故障诊断的新方法，该方法利用振动信号能量的时频域分布特征与瞬时相位波动特征可以进行精确的齿轮故障诊断，并通过与传统的频域诊断方法的比较，揭示出齿轮振动中存在的明显的非线性与非平稳特征，说明了不加选择地利用频域分析方法进行齿轮故障诊断很可能会得到错误的诊断结果；提出了一种轴承故障诊断的新方法，该方法利用ATVFD分解与Hilbert变换进行包络解调，并对解调得到的包络信号进行时频域分析，可以实现更加准确轴承故障诊断，说明了故障对载波信号的调制往往也是非平稳与非线性的；提出了一种利用振动信号进行柴油机汽缸内压力检测的新方法，柴油机汽缸压力的变化是柴油机故障诊断的重要参数，利用振动方法可大大简化汽缸压力的检测过程，该方法也是综合利用ATVFD分解与时频域分析方法实现的。

In the second part of of this thesis, we firstly derivate the operation condition of intracavity SHG of dualwavelength laser from the rate equation, and then numerically calculate the relationship between the laser operation parpmeters and the pumping powers. And then we discuss two possible ways to get balancing outputs of the harmonic waves of the two wavelengths. By comparing the two ways, we find that the method by increasing the geometrical factor of 1079. 5nm SHG has the higher efficiency than the method by adding the additional loss of 1079. 5nm basic wave. Besides, the first method is easier to realize in experiment than the second method.

本论文第二部分从速率方程出发，通过求解速率方程的稳态解得到了Nd：YAP腔内倍频双波长激光的连续运转条件，数值计算得到了随泵浦功率变化的激光运转情况；计算、分析了通过增加主波长的倍频耦合几何因子和增加主波长的腔内损耗两种方法获得均衡的双波长倍频输出的运转情况，通过二者的比较，发现增加倍频耦合几何因子的方法比增加损耗的方法效率更高，而且在实验上更易于实现。

When changing diaphragms in most drivers the new diaphragm may not be 100 % concentric to the old one, and the only way to align it in the magnetic gap is by removing the iron pole from the driver and loosening the screws holding the center pole piece, then and only then the diaphragm can be aligned with the center pole piece and rubbing or touching of the voice coil with the pole pieces in the gap is avoided, this may happen at certain frequencies only, producing a very harsh sound, even passing a static or a dc diaphragm test , therefore the claim that the diaphragms are replaceable in the field is not in many cases true, only when the driver has adjustable screw and dowel locating pin the alignment is possible without getting inside the driver and when alignment of the diaphragm is needed, the plate holding the pole pieces and assembly needs removal, by doing so the pole piece may be well set and aligned but the magnetic field will weaken significantly by such a procedure and solving one problem leads to another one, in this case the driver needs to be re magnetized in order to perform well.

当改变隔膜在大多数司机的新膜片不得100 ％同心旧的，只有这样，才能使其在磁场的差距是通过消除铁柱从司机和松动的螺丝中心柱举行一块，然后才是膈肌可配合中心极片和摩擦或触摸的音圈与极片的差距是可以避免的，可能会遇到这种情况只有在特定的频率，产生了非常严厉的声音，甚至通过一个静态或直流隔膜测试，因此声称隔膜的更换在外地不是在许多情况下，真实的，只有当该名司机已可调螺丝和销子定位销调整是有可能没有得到内部的驱动器和调整时，膈肌是必要的，举行的板极件和大会需要搬迁，这样做极可能是一块很好设置和调整，但磁场将削弱显着这样的程序和解决的一个问题导致另一次，在这种情况下，司机必须重新磁化，以表现良好。

It is indeed the product of various countries' culture, so its application is determined by cultural factors. The paper can be divided into three parts, the first part reviews Leech politeness principle and it's advantage and disadvantage, then it discusses its cultural characteristics by using contrastive analysis method to analyze cultural origins and decisiveness of politeness principle. Then it explains cultural decisiveness of the politeness principle by introducing Chinese politeness criteria, Examples on vocatives, terms of humility and honorifics, taboos and euphemism are also given to further illustrate this point. Finally it proposes the conclusion: we should learn more about politeness principle and its cultural characteristics and raise people's consciousness on this issue so as to help people successfully in cross-cultural communication.

全文主要分为三部分，第一部分回顾了Leech的礼貌原则的内容并对礼貌原则的合理之处和不足之处进行了分析；然后采用了对比分析的方法，从礼貌的文化渊源和礼貌的文化决定性来讨论礼貌的文化特性；接着引入顾曰国的汉语礼貌准则，以及从称呼语、恭维自谦语、禁忌委婉语方面举例来说明礼貌原则具有文化决定性；最后提出结论：应对礼貌原则及其文化特性进行研究以此提高人们的跨文化意识，促进跨文化交际的顺利进行。

The humidity resistance and the sensitivity to chemical warfare agents simulants of the tuned films were also investigated.Self-assembled LBL polyaniline films were prepared from aniline monomers by in situ polymerization and the mechanism of film formation was discussed. It was proposed that the self-assembly of polyaniline occurred through the quick absorption of aniline monomer cations or aniline monomer cation radicals on the glass surfaces by electrostatic attractions to form homogeneous polymerization centers, then increased gradually by the way of upright to the glass supports for extensity, leading to the polyaniline chain growth. The polyaniline was doped under acidic condition to absorb anionic polyelectrolyte, such as sodium polystyrene sulfonate based on the electrostatic force, or to adsorb polymer based on hydrogen-bonding interactions, for example Polyvinyl Alcohol, and then recycled to fabricate_n or_n self-assembled multilayer films.

跟踪了原位聚合的具有纳米结构的聚苯胺多层自组装膜的成膜过程，提出了该自组装膜的成膜机理：聚合反应初始阶段的苯胺阳离子或苯胺阳离子自由基通过静电作用快速吸附到带负电荷的基片表面，形成均匀的聚合中心，然后以垂直于基片的方式进行链增长生成PANI；PANI在酸性条件下经现场掺杂后带正电荷，再通过静电作用吸附带负电荷的聚电解质如聚苯乙烯磺酸钠，或通过氢键作用吸附聚合物如聚乙烯醇，这样循环LBL-SA即得到_n或_n等原位聚合聚苯胺自组装膜。

Lamblia were cultivated axenically with modified TYI-S-33 medium contained dihydroartemisinin (0.002 mg/L) for 12 h and then stained by rhodanmine phalloidin and paclitaxel (P22310). The cytoskeletons of the organisms were detected and analyzed by flow cytometry, and then observed by confocal microscopy.RESULTS: The cytoskeleton of trophozoites was markedly damaged after treatment with 0.002 mg/L dihydroartemisinin for 12 h.

将用含双氢青蒿素的改良TYI-S-33培养基培养后的蓝氏贾第鞭毛虫滋养体，用专一性结合f-肌动蛋白的荧光染料罗丹明－鬼笔环肽(rhodanmine phalloidin， RDP)标记微丝，结合微管的紫杉醇(Paclitaxel， Oregen Green 488， P22310)标记微管，用流式细胞术检测、分析，用激光共聚焦显微镜(confocal microscopy， CFM)观察滋养体微丝和微管的变化。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。