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The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The adhesion region between two adjacent teeth on the 3D dental model is first detected by exploiting the minimum principal curvature information. Then, the adhesion region is cut off and the tooth profile opening caused by the removal of the adhesion region is filled with triangular patches adopting a local optimization scheme, it is then refined to form an intermediate restoration surface patch. The final restoration result is obtained by minimizing the surface energy to reflect the bio-characteristics of the single tooth.

根据三维牙颌模型的形态特征，采用基于曲面最小主曲率的方法对粘连区域进行准确的检测提取；对删除齿间粘连区域后生成的齿间开口利用局部最优化权值规则进行剖分并细分优化，生成"中间"恢复曲面；根据牙齿的生理医学特征，以齿间开口的边界信息为约束条件，采用基于最小化曲面能量函数的方法对"中间"恢复曲面进行变形调整，实现单颗牙齿缺失形状的准确恢复。

In global mode, annular omnidirectional images are captured by using skip subsampling mode to guarantee high frame rate, and then unwrapped to cylindrical panoramic images containing global scene information of 360 degrees. In local mode, the location and size of sampling window of image sensor are reconfigured according to the position of interested region, thereby only a corresponding rectangle region of omnidirectional images is captured by using pixel-by-pixel mode, and then unwrapped to a high- definition local perspective image of the corresponding view angle. Finally, a hardware design based on DSP+FPGA is presented.

在全局模式下，为保证采集帧速率，使用行列跳跃模式采集完整的环状全向图，并将其展开为包含360°全局信息的柱面全景图像；在局部模式下，根据感兴趣区域的空间位置设置采样窗口大小及位置，以逐像素采样模式只采集全向图中相应的一块矩形区域，并将其展开为对应视线方向上高清晰度的局部透视图；最后给出了DSP+FPGA结构的系统硬件设计方案。

Data of tooth surface before and after wear were both obtained through laser scan system. The images were then reconstructed by SURFACER software.(2) C language program used of improved iterative closest point algorithms was developed to registrate imagines before and after wear. Standard plan was defined to the overlapped imagines after registration, then wear quantity was measured.￤ear was imitated by tooth brush machine which was made by STOMATOLOGY COLLEGE FORTH MILITARY MEDICAL UNIVERSITY.

应用三维激光扫描仪采集牙模型的表面数据，在计算机中用SURFACER逆向工程软件重建牙表面；②运用最近邻点迭代匹配算法加以改进，编制 C语言程序，配准磨耗前后的三维图像，在确定基准平面后，计算磨耗前后的体积变化量；③用口腔医院自制刷牙机进行模拟磨耗，用传统称重法，通过电子天平测试件的重量变化，用失水法计算测试材料的密度，换算成体积。

At first the contest is carried on by individual labourers, then by the workpeople of a factory,then by the operatives of one trade, in one locality, against the individual bourgeois who directly exploits

最初，斗争是由单个的工人进行的，然后是某一工厂的工人，然后是某一地方的某一劳动部门的工人，反对直接剥削他们的单个资产者。

At first the contest is carried on by individual labourers, then by the workpeople of a factory, then by the operatives of one trade, in one locality, against the individual bourgeois who directly exploits them.

最初是单个的工人，然后是某一工厂的工人，然后是某一地方的某一劳动部门的工人，同直接剥削他们的单个资产者作斗争。

He had sold the last of his furniture, then all duplicates of his bedding,his clothing and his blankets, then his herbariums and prints; but he still retained his most precious books, many of which were of the greatest rarity, among others, Les Quadrins Historiques de la Bible, edition of 1560; La Concordance des Bibles, by Pierre de Besse; Les Marguerites de la Marguerite, of Jean de La Haye, with a dedication to the Queen of Navarre; the book de la Charge et Dignite de l'Ambassadeur, by the Sieur de Villiers Hotman; a Florilegium Rabbinicum of 1644; a Tibullus of 1567, with this magnificent inscription: Venetiis,in aedibus Manutianis; and lastly, a Diogenes Laertius, printed at Lyons in 1644, which contained the famous variant of the manuscript 411, thirteenth century, of the Vatican, and those of the two manuscripts of Venice, 393 and 394, consulted with such fruitful results by Henri Estienne, and all the passages in Doric dialect which are only found in the celebrated manuscript of the twelfth century belonging to the Naples Library.

他把最后的几件木器也卖了，随后，凡属多余的铺盖、衣服、毛毯等物，以及植物标本和木刻图版，也全卖了；但是他还有些极珍贵的藏书，其中有些极为稀有的版本，如一五六○年出版的《历史上的圣经四行诗》，皮埃尔·德·贝斯写的《圣经编年史》，让·德·拉埃写的《漂亮的玛格丽特》，书中印有献给纳瓦尔王后的题词，贵人维里埃－荷特曼写的《使臣的职守和尊严》，一本一六四四年的《拉宾尼诗话》，一本一五六七年迪布尔的作品，上面印有这一卓越的题铭：&威尼斯，于曼奴香府&，还有一本一六四四年里昂印的第欧根尼·拉尔修①的作品，在这版本里，有十三世纪梵蒂冈第四一一号手抄本的著名异文以及威尼斯第三九三号和三九四号两种手抄本的著名异文，这些都是经亨利·埃斯蒂安②校阅并取得巨大成绩的，书中并有多利安方言的所有章节，这是只有那不勒斯图书馆十二世纪的驰名手抄本里才有的。

He had sold the last of his furniture, then all duplicates of his bedding,his clothing his blankets, then his herbariums prints; 8ttt8.com he still retained his most precious books, many of which were of the greatest rarity, among others, Les Quadrins Historiques de la Bible, edition of 1560; La Concordance des Bibles, by Pierre de Besse; Les Marguerites de la Marguerite, of Jean de La Haye, with a dedication to the Queen of Navarre; the book de la Charge et Dignite de l'Ambassadeur, by the Sieur de Villiers Hotman; a Florilegium Rabbinicum of 1644; a Tibullus of 1567, with this magnificent inscription: Venetiis,in aedibus Manutianis; lastly, a Diogenes Laertius, printed at Lyons in 1644, which contained the famous variant of the manuscript 411, thirteenth century, of the Vatican, those of the two manuscripts of Venice, 393 394, consulted with such fruitful results by Henri Estienne, all the passages in Doric dialect which are ssbbww.com found in the celebrated manuscript of the twelfth century belonging to the Naples Library.

他把最后的几件木器也卖了，随后，凡属多余的铺盖、衣服、毛毯等物，以及植物标本和木刻图版，也全卖了；但是 dddTt 他还有些极珍贵的藏书，其中有些极为稀有的版本，如一五六○年出版的《历史上的圣经四行诗》，皮埃尔·德·贝斯写的《圣经编年史》，让·德·拉埃写的《漂亮的玛格丽特》，书中印有献给纳瓦尔王后的题词，贵人维里埃－荷特曼写的《使臣的职守和尊严》，一本一六四四年的《拉宾尼诗话》，一本一五六七年迪布尔的作品，上面印有这一卓越的题铭：&威尼斯，于曼奴香府&，还有一本一六四四年里昂印的第欧根尼·拉尔修①的作品，在这版本里，有十三世纪梵蒂冈第四一一号手抄本的著名异文以及威尼斯第三九三号和三九四号两种手抄本的著名异文，这些都是经亨利·埃斯蒂安②校阅并取得巨大成绩的，书中并有多利安方言的所有8 tt t8.com 章节，这是只有那不勒斯图书馆十二世纪的驰名手抄本里才有的。

With the trend of changing structure of population and age, many 50-to-64-year-old people of Mature age generation are undergoing the change of life and meeting the turning points such as midlife crisis, empty nest syndrome and gerontic panic. Nearly half of the people of Mature age generation consider their lives to be hard and full of pressure. Therefore, this study is to classify the numerous Healing toys on the market by KJ method, then using the Delphi method to understand the healing characteristics of those toys and related discussion by an interview with experts, and then investigating the people of Mature age generation's perspectives by the questionnaire.

随著社会人口结构变化，许多50至64岁的「熟龄族」正经历著人生许多的改变与转捩点，几近半数的熟龄族认为他们生活辛苦且压力庞大，因此本研究主要先以KJ法将市面上众多的疗伤系玩具进行分类，再以Delphi法专家调查来了解疗伤系玩具的特性与相关探讨，并用问卷调查的方式来得知熟龄族对其疗伤感的看法，找寻熟龄族更多的抒压管道与可能性。

In dark and hardship,I and my sworb was surrounded by bloody.I who just dispatched troops to fight was surrounded by two monsters.My body was rended by their sharp teech,my blood burested forth.I tried my best to raise my sworb and irritated the monster's hearted abruptly.Then,I didn't have any power to brandish my sworb. My blood running off and my consciousness was disppearing.In a twin kling,I daw a sword sliced the other monster's head,then I fainted.

黑夜，风高。血腥笼罩着提着长剑的我，才出道的我被两个怪物围攻，我身上的血肉被他们锋利的牙齿撕裂，鲜血飞溅出来，我拼尽全力提起手中的长剑，咬咬牙，狠狠地刺穿一只怪物的心脏，此时的我，已经没有任何力气挥动那柄沉重的长剑，我的血液在流淌，我的意识在消亡，在我昏倒的一瞬间，我看见一柄长剑从后面削掉了那怪物的脑袋。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。