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Eagles--Last Good Time In Town [00:20.02]1223_dd制作 [00:58.45]I like to go out every now and then [01:02.30]I can't wait to do it again [01:06.19]But I haven't had the time [01:10.81]Lately [01:14.27]I like to step out every once in a while [01:18.89]I kind of like to do it in style [01:22.90]I haven't had the time [01:26.96]Lately [01:31.70]Lately I've been stayin' at home [01:35.60](Stayin' at home, stayin' at home) [01:39.73]Workin' the crosswords, turn off the phone [01:43.85](Turn off the phone, turn off the phone) [01:51.71]And I dream I'm on vacation [01:55.86]'Cause I like the way that sounds [01:59.98]It's a perfect occupation [02:04.27]For me [02:08.90]I don't mind being by myself [02:12.72]If there's no one else around [02:16.69]It's the last good time in town [02:38.59]Well, I could stand up straight, get a real job [02:43.25]Stay up late with the same old mob [02:47.16]But it hasn't crossed my mind [02:51.79]Lately [02:55.97]Put on a suit, work downtown [03:00.12]Then come up when the sun goes down [03:03.88]No, I haven't had the time [03:08.50]Lately [03:12.70]Lately I've been stayin' at home [03:20.37](Stayin' at home, stayin' at home) [03:21.06]Closin' the curtains, play jazz on trombone [03:24.95](Jazz on trombone, jazz on trombone) [03:32.55]And I dream I'm on vacation [03:36.79]No, you won't see me around [03:41.00]It's a perfect situation [03:45.37]For me [03:49.77]I don't mind being by myself [03:53.68]'Cause I know you'll come on down [03:57.59]It's the last good time in town [05:12.71]Lately I've been stayin' at home [05:16.58](Stayin' at home, stayin' at home) [05:20.72]Workin' the crosswords, turn off the phone [05:24.88](Turn off the phone, turn off the phone) [05:32.65]And I dream I'm on vacation [05:36.68]'Cause I like the way that sounds [05:40.96]It's a perfect occupation [05:45.20]For me, yes it is [05:49.85]I don't mind being by myself [05:53.75]If you don't see me around [05:57.75]It's the last good time, last good time in town

钛：最后好时光在城市 [按：1223_dd] [00：00.01]鹰-最后好时光在城 [00：20.02] 1223_dd制作 [00：58.45]我想现在出去，然后每 [01：02.30]我不能等待再次做 [01：06.19]但我没有时间 [01：10.81]最近 [01：14.27]我想退了，而在每一次 [01：18.89]我有点像做有声有色 [01：22.90]我没有时间 [01：26.96]最近 [01：31.70]最近我一直留驻'在家 [01：35.60]（留驻'在家里，留驻'在家） [01：39.73] Workin'的纵横字谜，关掉手机 [01：43.85]（关闭手机关闭，关闭手机） [01：51.71]我的梦想我在我休假 [01：55.86]'原因我很喜欢的声音 [01：59.98]这是一个完美的职业 [02：04.27]对我来说 [02：08.90]我不介意由我正 [02：12.72]如果没有其他人靠近 [02：16.69]这是最后一次在城里好 [02：38.59]嗯，我可以挺直腰杆，得到一份真正的工作 [02：43.25]保持与老暴徒晚 [02：47.16]但一直没有干扰过我 [02：51.79]最近 [02：55.97]关于提起诉讼，把工作市中心 [03：00.12]然后提出时，天黑了 [03：03.88]没有，我没有时间 [03：08.50]最近 [03：12.70]最近我一直留驻'在家 [03：20.37]（留驻'在家里，留驻'在家） [03：21.06] Closin'的窗帘，玩爵士长号 [03：24.95]（爵士乐长号，爵士的长号） [03：32.55]我的梦想我在我休假 [03：36.79]不，你不会看到我周围 [03：41.00]这是一个完美的情况 [03：45.37]对我来说 [03：49.77]我不介意由我正 [03：53.68]'的原因，我知道你会来的下降 [03：57.59]这是最后一次在城里好 [05：12.71]最近我一直留驻'在家 [05：16.58]（留驻'在家里，留驻'在家） [05：20.72] Workin'的纵横字谜，关掉手机 [05：24.88]（关闭手机关闭，关闭手机） [05：32.65]我的梦想我在我休假 [05：36.68]'原因我很喜欢的声音 [05：40.96]这是一个完美的职业 [05：45.20]对我来说，是有 [05：49.85]我不介意由我正 [05：53.75]如果你看不到我周围 [05：57.75]这是最后的好时机，最后在城里的好时机

Methods we build rat retinal cells and rgcs cultured in virto by high pressure.put 80 mmhg pressure to rgcs in close tightly culture bottle for four hours, then collect rgcs and check the sum of apoptosis cells by flow cytometry.put the pressure 80 mmhg to rgcs in close tightly culture bottle for four hours,then collect rgcs and look for the apoptotic gene by gene chip.

（1）在rgcs中加入dsx，加压培养后收集细胞，用流式细胞仪测定凋亡细胞百分率。（2）在rgcs中加入dsx，加压培养后收集细胞，用基因芯片寻找凋亡基因的表达。结果（1）在rgcs高压培养时，dsx组的凋亡百分率低于模型组和对照组。

Here Pilgrims roam, that stray'd so farr to seek In Golgotha him dead, who lives in Heav'n; And they who to be sure of Paradise Dying put on the weeds of Dominic, Or in Franciscan think to pass disguis'd; [ 480 ] They pass the Planets seven, and pass the fixt, And that Crystalline Sphear whose ballance weighs The Trepidation talkt, and that first mov'd; And now Saint Peter at Heav'ns Wicket seems To wait them with his Keys, and now at foot [ 485 ] Of Heav'ns ascent they lift thir Feet, when loe A violent cross wind from either Coast Blows them transverse ten thousand Leagues awry Into the devious Air; then might ye see Cowles, Hoods and Habits with thir wearers tost [ 490 ] And flutterd into Raggs, then Reliques, Beads, Indulgences, Dispenses, Pardons, Bulls, The sport of Winds: all these upwhirld aloft Fly o're the backside of the World farr off Into a Limbo large and broad, since calld [ 495 ] The Paradise of Fools, to few unknown Long after, now unpeopl'd, and untrod; All this dark Globe the Fiend found as he pass'd, And long he wanderd, till at last a gleame Of dawning light turnd thither-ward in haste [ 500 ] His travell'd steps; farr distant he descries Ascending by degrees magnificent Up to the wall of Heaven a Structure high, At top whereof, but farr more rich appeer'd The work as of a Kingly Palace Gate [ 505 ] With Frontispice of Diamond and Gold Imbellisht, thick with sparkling orient Gemmes The Portal shon, inimitable on Earth By Model, or by shading Pencil drawn.

此外还有很多，说起来未免太冗长，他们无非是些未成熟的、痴呆的、穿着黑、白、灰色衣的身带骗人的假法宝的隐士和托钵僧。其中有的在这里云游巡礼，曾到各各他去寻觅那活在天上的死人，临终穿上圣多明我派或圣方济派的袈裟，以证明自己确实去过极乐天堂，他们误以为这样打扮就可畅通无阻。他们通过七星天，通过"恒星天"，通过那权衡黄道振动均势的"水晶天"，并且还通过"原动天"。圣彼得手拿钥匙，站在天堂的边门口，像是在等待他们，正要举步登上天堂的阶梯时，看吧！从左右吹来一阵猛烈的横风，他们被斜吹到十万里外的远空中去：那时看见僧帽、头巾、袈裟，连同它们的穿戴者一起被吹翻扯烂，还有圣骨、念珠、免罪券、特免证、赦罪证、训谕，全被高高卷起，都成了风的玩具，他们飘过这世界的背面而远落在广大的地狱边缘，被称"愚人的乐园"，很久之后将是家喻户晓的，但是现在却是人迹罕至的地方。魔王走过这整个黑球，经过长久的漫游，终于发现一线曙光，便加紧他的步伐，向那边走去。他远远望见一座高大的建筑，从它那宽大的阶梯拾级而上，可以抵达天国的城垣，它的顶上，像有比王宫还要富丽的宫门，它的正面镶有金刚石和黄金；大门上密饰着东方的珠宝，光彩夺目，人间的浓淡画笔也无法描绘它。

On this basis a design scheme of the database for SCADA based on the component object model is presented, in which the content and background of IEC 61970 standard is introduced first, then some different component model are assayed , then a design scheme of the database for SCADA based on the component object model is presented, the foreground application of SCADA is connected with the database by components.The new model have some advantages: system have disrelation with develop language ,the peculiarity of COM make the system fit in with different apply program developed with different language;(2) the system can be modified and upgraded easily by means of upgrading the component modules;(3) the system can be enlarged easily by means of adding the new component modules without quiet amendment in old system.

为此，本文介绍了一种基于组件对象模型的SCADA数据库系统设计方案，文中首先介绍了IEC61970标准的内容及产生背景，分析了几种不同的组件模型，在此基础上，提出了一种基于COM组件技术的SCADA数据库设计及访问模式，在此模式下根据SCADA数据库不同的功用划分了不同的组件模块，利用组件连接数据库与SCADA前台应用，基于组件技术的SCADA数据库设计有以下一些特点：(1)与语言无关性，COM的特性决定了此系统上可以应用不同语言开发的应用程序；(2)系统易修改及维护，用户可以通过更新组件模块的方式修改及维护系统；(3)系统易扩展新的功能，用户可以通过开发新的组件方式将新的应用与原系统结合，而不用对原系统做很大的修改。

He had sold the last of his furniture, then all duplicates of his bedding,his clothing and his blankets, then his herbariums and prints; but he still retained his most precious books, many of which were of the greatest rarity, among others, Les Quadrins Historiques de la Bible, edition of 1560; La Concordance des Bibles, by Pierre de Besse; Les Marguerites de la Marguerite, of Jean de La Haye, with a dedication to the Queen of Navarre; the book de la Charge et Dignite de l'Ambassadeur, by the Sieur de Villiers Hotman; a Florilegium Rabbinicum of 1644; a Tibullus of 1567, with this magnificent inscription: Venetiis,in aedibus Manutianis; and lastly, a Diogenes Laertius, printed at Lyons in 1644, which contained the famous variant of the manuscript 411, thirteenth century, of the Vatican, and those of the two manuscripts of Venice, 393 and 394, consulted with such fruitful results by Henri Estienne, and all the passages in Doric dialect which are only found in the celebrated manuscript of the twelfth century belonging to the Naples Library.

他把最后的几件木器也卖了，随后，凡属多余的铺盖、衣服、毛毯等物，以及植物标本和木刻图版，也全卖了；但是他还有些极珍贵的藏书，其中有些极为稀有的版本，如一五六○年出版的《历史上的圣经四行诗》，皮埃尔·德·贝斯写的《圣经编年史》，让·德·拉埃写的《漂亮的玛格丽特》，书中印有献给纳瓦尔王后的题词，贵人维里埃－荷特曼写的《使臣的职守和尊严》，一本一六四四年的《拉宾尼诗话》，一本一五六七年迪布尔的作品，上面印有这一卓越的题铭："威尼斯，于曼奴香府"，还有一本一六四四年里昂印的第欧根尼·拉尔修①的作品，在这版本里，有十三世纪梵蒂冈第四一一号手抄本的著名异文以及威尼斯第三九三号和三九四号两种手抄本的著名异文，这些都是经亨利·埃斯蒂安②校阅并取得巨大成绩的，书中并有多利安方言的所有章节，这是只有那不勒斯图书馆十二世纪的驰名手抄本里才有的。

He had sold the last of his furniture, then all duplicates of his bedding,his clothing his blankets, then his herbariums prints; 8ttt8.com he still retained his most precious books, many of which were of the greatest rarity, among others, Les Quadrins Historiques de la Bible, edition of 1560; La Concordance des Bibles, by Pierre de Besse; Les Marguerites de la Marguerite, of Jean de La Haye, with a dedication to the Queen of Navarre; the book de la Charge et Dignite de l'Ambassadeur, by the Sieur de Villiers Hotman; a Florilegium Rabbinicum of 1644; a Tibullus of 1567, with this magnificent inscription: Venetiis,in aedibus Manutianis; lastly, a Diogenes Laertius, printed at Lyons in 1644, which contained the famous variant of the manuscript 411, thirteenth century, of the Vatican, those of the two manuscripts of Venice, 393 394, consulted with such fruitful results by Henri Estienne, all the passages in Doric dialect which are ssbbww.com found in the celebrated manuscript of the twelfth century belonging to the Naples Library.

他把最后的几件木器也卖了，随后，凡属多余的铺盖、衣服、毛毯等物，以及植物标本和木刻图版，也全卖了；但是 dddTt 他还有些极珍贵的藏书，其中有些极为稀有的版本，如一五六○年出版的《历史上的圣经四行诗》，皮埃尔·德·贝斯写的《圣经编年史》，让·德·拉埃写的《漂亮的玛格丽特》，书中印有献给纳瓦尔王后的题词，贵人维里埃－荷特曼写的《使臣的职守和尊严》，一本一六四四年的《拉宾尼诗话》，一本一五六七年迪布尔的作品，上面印有这一卓越的题铭："威尼斯，于曼奴香府"，还有一本一六四四年里昂印的第欧根尼·拉尔修①的作品，在这版本里，有十三世纪梵蒂冈第四一一号手抄本的著名异文以及威尼斯第三九三号和三九四号两种手抄本的著名异文，这些都是经亨利·埃斯蒂安②校阅并取得巨大成绩的，书中并有多利安方言的所有8 tt t8.com 章节，这是只有那不勒斯图书馆十二世纪的驰名手抄本里才有的。

The web paper is inputted by Mill Roll Stand, and after heated and regulating the moisture by the pre-heater, the paper is put into the Single Facer for shaping and then conglutinating with another top paper to form a single corrugated board. The single corrugated board is then transmitted by the overhead bridge conveyor to multiple heater to be reheated, after this the paper is to double facer for being heated and jointed, herein a continuous corrugated board is formed.

由原纸架输入卷形原纸，经过热缸机加热及调节湿度，进入单面瓦楞机，经过瓦楞机的滚轧成形，与另一面纸粘合成单面瓦楞纸，由天桥架传送，经多重预热器再次加热，经上浆机粘糊再至双面机加热贴合，此时连续的瓦楞纸板即已成形。

The control principle of electrostatic shaping was introduced according to the balance between electrostatic force and resulting force formed by membrane deformation and the complex shaping process of SMEC. Then, taking the trisection circularity electrode for an example, the distribution characteristic of electric potential in the electrostatic field was analyzed, namely, the expression of potential function in the electrostatic field was deduced by Laplacian equation. And then, by combining the difference equation with electric potential expression, the numerical solutions of electrostatic force in single electrode mode and trisection circularity electrode mode were disposed.

根据静电力与薄膜变形载荷作用力之间的平衡关系和静电拉伸薄膜反射镜成形的复杂过程，介绍了薄膜反射镜静电成形的控制原理；以三等分环状电极为例，分析了静电场中空间电势分布特性，即从拉普拉斯方程推导出静态场势函数的表达式；然后，利用差分与电势方程结合的方法，对单电极电场力和三等分环状电极电场力进行了数值求解；最后，将计算面形与理想抛物面进行了比较，结果显示，单电极情况下得到的薄膜反射镜面形不是理想抛物面，若采用多电极控制可获得更高的控制精度。

In combination with the crushed rock slope as an example, the allocation of an anchorage force on longitudinal beams and transverse beams is calculated by adopting the distribution coefficient law of the nodal form at first. Then the internal forces of the lattice beam are calculated respectively through the software on the basis of Winkler elastic foundation model and the semi-infinite foundation model, and then compared with the internal forces which are based on simplified foundation pressure calculation. The active pressure of rock is calculated approximately by Ranking soil pressure theory in the working stage. Sequentially, the reinforcing bars are disposed by two-stage's averagely calculated values.

以破碎岩质边坡为例，采用节点荷载分配法计算了锚索锚固力在格构梁纵、横梁之间的分配比率，然后基于Winkler弹性地基模型和弹性半空间地基模型分别计算格构梁在张拉阶段的内力，并与采用简化的地基反力计算值对比；采用朗肯土压力理论近似计算在工作阶段主动岩体压力，将地梁视为在线性分布力作用下倒置于坡面上的连续梁，按力矩分配法计算地梁内力，取两阶段计算平均值进行配筋。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。