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Major content of this work includes five aspects as follows:(1). Oxygen sensitive materials had been prepared by carrier covalence method, and the preparation mechanism of the materials had been investigated by FI-IR, SEM, and the detection experiment of oxygen. Then through the detection of oxygen, the sensitivity and stability had been estimated, and it was studied that the properties of materials would be affected by the various factors.(2). Oxygen sensitive materials had been prepared by carrier covalence-cross linking method, and the preparation mechanism of the materials had been investigated by FI-IR, SEM, and the detection experiment of oxygen. Then through the detection of oxygen and spectrophotometer, the sensitivity and stability had been estimated, and it was studied that the properties of materials would be affected by the various factors.(3). For the detection of gaseous oxygen, the fiber optical sensor on basis of oxygen sensitive materials had a respond time of 10s, detection limit of 5ppmand detection precision of 0.5%. The sensor had good repeatability and stability, a less delay and at least 1 year life-span.

本论文主要包括以下五个方面的内容：(1)载体共价法制备氧敏感材料：通过红外光谱、扫描电镜图谱和氧测试实验探讨该氧敏感材料的制备机理，通过氧测试实验评价该氧敏感材料的氧敏感性和稳定性，同时研究了各种因素对该氧敏感材料性能的影响(2)载体共价—交联法制备氧敏感材料：通过红外光谱、扫描电镜图谱和氧测试实验探讨该氧敏感材料的制备机理，通过氧测试实验和分光光度计评价该氧敏感材料的氧敏感性和稳定性，同时研究了各种因素对该氧敏感材料性能的影响(3)氧敏感材料在光纤气态氧传感器中的应用：该传感器的响应时间为10s，检测下限为5ppm，检测精度为0.5％，具有较好的重复性和稳定性，迟滞较小，使用寿命至少为1年，适合各种环境下气态氧浓度的检测。

In this paper,the nonstandard analysis theory is used for inducing a metric space by a Loeb measure space.On this basis,a metric space is induced by a internal finitely additive measure space.The close relationship between the metric space induced by a Loeb measure space and the metric space induced by a internal finitely additive measure space is illustrated with the concepts and some properties of Loeb measure.Then,some properties of the metric space that induced by a internal finitely additive measure space are studied.In the first two chapters,we first Succinctly present the origin,development and research states of the nonstandard analysis.Then,the theoretical foundation of nonstandard analysis as well as the axiomatic nonstandard analysis are given.Finally, the nonstandard model and the saturation model are discussed,as well as some natures of the nonstandard model and several equivalent conditions of saturation model are given.

本文利用非标准分析理论，在由Loeb测度空间导出度量空间的基础上，由内有限可加测度空间导出了度量空间，并借助Loeb测度的概念和若干性质证明了由标准的测度空间导出的度量空问和由内有限可加测度这个非标准的测度空间导出的度量空间有着密切的关系，在此关系的基础上还研究了由有限可加测度这个非标准的测度空间导出的度量空间的性质在第一、第二章里，我们首先简单介绍了非标准分析的产生、发展及研究现状，接着给出了非标准分析的理论基础以及公理化的非标准分析，进而讨论了非标准模型和饱和模型，并给出了非标准模型的一些性质和饱和模型的若干等价条件。

P你好lomela king pandion of athens had two daughters,procne and p你好lomela.when athens was threatened by the wild men,king tereus of thrace came to its help.out of gratitude king pandion offered tereus either of 你好s daughters in marriage and the thracian king chose procne as wife.for yearsthey lived in thrace and had one son,itylus by name.then procne became homesick and longed to see her dear sister p你好lomela.at her repeated requests tereus sailed to athens tofetch p你好lomela.on the way back 你好s evil heart took flame at the sight of p你好lomela who was then in her beauty of maidenhood.he seized and carried her away by force,cut out her tongue and imprisoned her in alonely hut in the woods.to procne he lied,saying that p你好lomela was dead.p你好lomela stayed in prison for ayear,where she had woven her painful story into the web of arobe.then she managed to send the robe to her sister.as soon as she received the web procne came over to the woods and to reher sister away from the keepers.

雅典国王潘底翁有两个女儿，普鲁丝妮和菲勒美拉当雅典受到生番的威胁时，色雷斯国王泰诺斯伸出了援助之手出于感激，国王潘底翁让泰诺斯从自己的女儿中任选一位作他妻子泰诺斯选择了普鲁丝妮他们在色雷斯生活了很多年，并且生了一个儿子，名叫意大拉斯由于阔别故土，普鲁丝妮患了想念家乡病，她非常想见亲爱的妹妹菲勒美拉在她的一再要求下，泰诺斯搭船去雅典接菲勒美拉来与她姐姐团聚在返回的途中，看到菲勒美拉浑身散发出的芳华和美丽，泰诺斯非常嫉妒，顿生歹意他强制进行带走了菲勒美拉，并割去了她的舌头，将她关在森林中一个荒僻的小茅屋里在普鲁丝妮面前，他撒谎说菲勒美拉已经死了菲勒美拉被囚禁了整整一年在囚禁期间，她将痛苦的经历编织成了一件长袍；然后，她想方设法让长袍落到她姐姐的手中普鲁丝妮一收到那件长袍就降临了森林，将她妹妹救了出来

Premier Wenjiabao, which benediction do you want to get in the new year? I make the cat scratch your nose by its tail, so you feel pruritic and sneeze. Then I bless you to your health. I make the cat scratch your nose by its tail again, so you feel pruritic and sneeze again. Then I bless you to your good mood. I make the cat scratch your nose by its tail again, so you feel pruritic and sneeze again. Then I bless you to your good luck and your glow of happiness. I thought your poem is very wonderful.

我让波丝猫咪用尾巴挠一下您的鼻子，您痒痒了，您打了一个喷嚏，糖果公主会说祝您健康；我让波丝猫咪用尾巴再挠一下您的鼻子，您又痒痒了，您又打了一个喷嚏，糖果公主会说祝您好心情；我让波丝猫咪用尾巴再挠一下您的鼻子，您还是痒痒了，您再打了一个喷嚏，糖果公主会说祝您红光满面，好运连连。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

And try to find out the effect of TSA on the proliferation and differentiation of rat tracheal stem cell. Methods 1.Preparation of 5-Fu Model and TSA treatment Two weeks" Wistar rats provided by the animal center of the China Medical University. The animals were sacrificed by 10% Chloral Hydrate 0.4ml/100g. Then the tracheas were excised aseptically. Then they were douched with PBS and cultured in 1:1 mix of Dulbecco"s modified Eagle"s medium and Ham"s F-12 medium (DMEM/F12) containing 120 mg/ml 5-Fu and 10% fetal bovine serum for 12h at 37°C. The tissues were then refreshed by culturing in DMEM/F12 containing 10% FBS and 200nM TSA.

1、制备气管损伤模型及剥离上皮取约200克左右的Wistar大鼠，雌雄不限(中国医科大学实验动物中心提供)，腹腔注射10%水合氯醛0.4ml/100g，无菌条件下取出气管，无菌PBS反复冲洗，洗净血液和粘液，置于DMEM/F12培养液中(含10%胎牛血清)，于培养液中加入终浓度为100mg/ml的5-Fu，37℃，5%CO2孵育12小时，弃去上述培养液，换成新鲜DMEM/F12液(含10%胎牛血清)，同时加入终浓度为200nM的TSA继续培养，于换液后3、6、12、24、48小时分别10%中性福尔马林固定，石蜡包埋，制成4μm厚的组织切片。

Methods: 2ethyl aniline was used as starting material of the synthesis and was processed by acetic anhydride acylation, strong nitric acid nitraction at low temperature, and desacyl synthesis to produce 2ethyl5nitro aniline. The product was then synthesized to 3methyl6nitro1Hindazole by using ringclosing reaction in the presence of NaNO2, and then ethylated to obtain 2,3diethyl6 nitro2Hindazole. The latter compound was then reduced by SnCl2/Hcl to get target compound 2,3diethyl6nitro2Hindazole.

以2乙基苯胺为原料经醋酐酰化、浓硝酸低温硝化及去酰基保护合成2乙基5硝基苯胺，后用亚硝酸钠关环得3甲基6硝基1H吲唑，经硫酸二甲酯甲基化得2，3二甲基6硝基2H吲唑，最后用氯化亚锡将其还原得到目标产物，通过1H NMR确定结构与目标产物一致。

Methods: 2-ethyl aniline was used as starting material of the synthesis and was processed by acetic anhydride acylation, strong nitric acid nitraction at low temperature, and desacyl synthesis to produce 2-ethyl-5-nitro aniline. The product was then synthesized to 3-methyl-6-nitro-lH-indazole by using ring-closing reaction in the presence of NaNO2, and then ethylated to obtain 2, 3-diethyl-6- nitro-2H-indazole. The latter compound was then reduced by SnC12/Hcl to get target compound 2, 3-diethyl-6-nitro-2H-indazole.

以2-乙基苯胺为原料经醋酐酰化、浓硝酸低温硝化及去酰基保护合成2-乙基－5-硝基苯胺，后用亚硝酸钠关环得3-甲基－6-硝基－1H-吲唑，经硫酸二甲酯甲基化得2，3-二甲基－6-硝基－2H-吲唑，最后用氯化亚锡将其还原得到目标产物，通过1HNMR确定结构与目标产物一致。

One is low valence vanadium oxide thin films were deposited by direct current facing target magnetron sputtering firstly, and then thermal oxidated; the other is high valence vanadium oxide thin films were deposited by ion beam sputtering, then thermal deoxidated. Vanadium oxide thin films with phase transition were obtained at the lower thermal process temperature 300℃, which is compatible with MEMS technologys. The composition of VO_x thin film fabricated by first method includes mainly VO_2, V2O3 and VO_, the TCR is -2.25%/K, the TCR gradually increases with phase transition temperature decreases. The composition of VO_x thin film fabricated by the second method includes mainly VO_2, V2O5 and V2O3 , and the resulting VO_x thin filmTCR is -3.0%/K. The TCR of phase transition vanadium oxide thin films made by second method is higher than that made by first method for the existance of V2O5 in thin films.

分别采用直流对靶磁控溅射制备低价态氧化钒薄膜再附加热氧化处理的方式，和射频离子束溅射制备高价氧化钒薄膜附加热还原处理的方式获得了具有相变特性的氧化钒薄膜，第一种方式获得相变特性氧化钒薄膜的热处理温度最低为300℃，克服了以往高温条件下热处理不能与MEMS工艺兼容的缺点；相变特性氧化钒薄膜的组分以VO_2为主，含有V2O3和VO_，室温TCR为-2.25%/K；室温电阻温度系数随相变温度的降低逐渐升高；采用第二种方式获得的相变氧化钒薄膜的组分以VO_2为主，含有V2O5和V2O3，室温TCR可达-3.0%/K；分析发现，具有相变特性的氧化钒薄膜中含有V2O5时，室温电阻温度系数明显增大。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。