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GCJ8 activate medium, preculture 2 days, 5 X Ca concentration in preculture medium and coculture medium, inoculation I mineture,4mg li1 AgN0 and 0.7% P\P, delay selection 5 days will obtain the highest transformation frequenc Using 'pingyitiancha'shoot apex, mediated by A.tum as above system, the HVAIgene, was transformed into pingyitiancha and obtained Kam resistance rerzeneration plants, it is confirmed that 6 plants of them is transformation plants by identified by PCR and dot blot

按照上述体系，以平邑甜茶茎尖分生组织为受体，将HVA1基因导入平邑甜茶中，获得了具有Kam抗性的再生植株，经PCR与点杂交鉴定，证实有6株为转化植株。

The first part consists of three lessons from Sacred Scripture (two chants and a prayer being interposed) which are followed by a long series of prayers for various intentions; the second part includes the ceremony of unveiling and adoring the Cross, accompanied by the chanting of the Improperia; the third part is known as the Mass of the Presanctified, which is preceded by a procession and followed by vespers.

第一部分包括三个教训，从神圣经文（两名呼喊和祈祷被中间人一样），这些是随后进行了一系列长期的祈祷，为各种意图；第二部分，包括颁奖典礼揭幕，并崇拜十字架上，伴以呼喊的该improperia ；第三部分是被称为群众的presanctified ，这是之前有一个游行，并随后晚祷。

When trading internationally, comparatively speaking, the delivery of goods involves many risks because of various ways of transporting by sea, by land or by air. In some cases, the goods to be delivered are in grave dangers, such as theft, fire, ship-sinking, breaking-up, leaking, putridness, deteropration and so on.

在国际货物买卖中，货物的交付相对比较复杂，而且多要经过海、路、空的长途运输，货物难免要面临灭失或损坏的风险，如盗窃、火灾、沉船、破碎、渗漏及不属于正常损耗的腐烂变质等。

The term software engineering was coined by FL Bauer and popularized by the 1968 NATO Software Engineering Conference, which was organized by Brian Randell.

软件工程的任期是由佛罗里达州鲍尔和推广由1968年北约软件工程会议，这次会议是由布莱恩兰德尔。

It was, however, the last phase of Buddhism in India, where the religion - partly by reabsorption into the Hindu tradition, partly by persecution by the Muslim invaders - ceased to exist by the 13th century.

不过，当时的最后一期的佛教在印度，那里的宗教-部分是由重吸收进入印度教的传统，这部分是由受迫害的穆斯林入侵者-不复存在，由1 3世纪。

It was, however, the last phase of Buddhism in India, where the religion - partly by reabsorption into the Hindu tradition, partly by persecution by the Muslim invaders - ceased to exist by the 13th century.

然而，它的最后阶段，佛教在印度，那里的宗教-部分重吸收进入印度教的传统，部分受迫害的穆斯林入侵者-不复存在了1 3世纪。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

We can define "covenant" as a mutually binding compact between God and His people, sovereignly transacted by the Lord, wherein a promise is made by God which calls for trust on the part of His people and entails obligations of submission which are sanctioned by blessings and curses.

我们可以把&圣约&定义为：对上帝和祂子民都具约束力的合同，完全由上帝至高主权所发起，其中有上帝的应许，呼召祂的子民信靠祂，并包含顺服的义务，也就是带有条件的祝福和咒诅。

Under the industrialization and market of water supply enterprise, it uses the tool of SFOT strategy analysis by the development of theory and policy of water supply. It puts up with market competition strategy of Shangrao water supply enterprise by strategetic analysis and matching. That is to say, by market penetration, market development and product development to make water supply enterprise grow densely. it will improve the assistance of the development of water supply by analysis of resources of strategy and combination of market.

在推进水业产业化和市场化的背景下，根据供水发展战略的理论与政策，运用SWOT战略分析工具，通过细致的战略分析与战略匹配，提出了上饶市自来水公司的市场竞争战略：即通过市场渗透、市场开发和产品开发策略使供水主业实现密集性成长；通过对企业战略资源的分析，结合市场情况，将大力推进供水辅业的快速、健康发展。

By synthesizing theories of systems engineering, science of talent, talent assessment, performance appraisal and bibliometrics, the author analyzes the qualification structure and performance structure of theachers in military academies and sets up a basic structure and concrete appraisal indicator system of qualifications of technical specialty for teachers in colleges. The author puts forward new appraisal indicators such as "lecuring hours coefficient","republication coefficient","thesis of core journals coefficient" and "citation coefficient". Comparatively complete quantification approaches are invented by the author for working performance of teaching, researching and social service of teachers. Based on this, the author establishes an integrated method to generate the appraisal results. By the application of stadardized processing on the appraisal results and synthetization of weighted indexes, the author solves the addibility and comparability problems of differentiated appraisal results by category or dimensiok, and realizes the amalgamation and comprehensive appraisal of inflexible appraisalresults and flexible appraisal results, and probes into the testification methods of validity, consistency and sensitivity analysis for the indicators system.

综合运用系统工程理论、人才学理论、人才素质测评理论、工作绩效评估理论、文献计量学等原理与方法，分析研究了军校教师的素质结构和绩效结构；建立了高校教师专业技术资格评审条件基本结构和具体评价指标体系，提出了&完成主讲课程课时系数&、&著作重版系数&、&核心期刊论文系数&、&论文被检索引用系数&等若干新的评价指标；提出了比较完整的教师的教学、科研、社会服务等工作业绩的量化方法；以此为基础，建立了评价结果的综合方法，通过对评价结果的标准化处理，运用指标加权综合法，解决了不同类别、不同量纲指标的评价结果的可加性和可比较性问题，实现了硬评价结果和软评价结果的融合和综合评价；探讨了指标体系有效性、一致性、灵敏度分析的检验方法。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。