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Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础,构建了原核表达载体PQE30a/FeSOD,经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后,再转化入大肠杆菌M15中,通过IPTG诱导,得到高效体外表达,经SDS-聚丙烯酰胺凝胶电泳检测,表达的目的蛋白占总菌体蛋白的37%,可溶性和包涵体两种形式均有存在,上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析,表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

On the basis of the conclusion above, the nonlinear weak controllable vectors can be obtained, which compose the second class of weak controllable matrix, by means of matrix transfermations of the first class of weak controllable matrix. Furthmore the second criterion of weak controllability, accessibility and strong accessibility, which is more standard, more succinct and more practical, is obtained. On the basis of the first criterion and the second criterion, several important problems about nonlinear controllability are discussed, which include 1 local controllability; 2 three classes of local controllable decomposition, separately by means of local coordinate changes in the neighborhood of a nonsingular point, a point on a singular surface or a designated point x〓 with a designated input u〓; 3 the character of controllability of the ith state variable x〓 in different controllable areas and their boundaries.

在第一判别定理和第二判别定理的基础上,我们分析了非线性系统受控性质中的几个重要问题,所得到的结论包括i得到了非线性系统在可平衡点附近的能控性判别定理;ii分别给出了非线性系统在非奇异点、s阶奇面以及在状态空间内给定的一点x〓和输入u〓上的三种状态分解形式以及相应的求解方法;利用系统的Ⅰ型弱能控矩阵与Ⅱ型弱能控矩阵秩相等的条件,得到了只用系统的前n个弱能控向量来判断系统的弱能控性的判别定理;iii通过引入系统状态分量的三类受控区域边界的定义及相应的求解方法,初步探讨了非线性系统各个状态分量在不同的受控区域及其边界上的受控性质。

The invention has the advantages that by means of single film growth craft, supporting structures of a pier, a bridge leg, and a deck are accomplished, thus greatly lowering the difficulty and complicity of the craft; the pier, the bridge leg, and the deck are formed into a trinity, thus assuring the mechanical support of the whole micro bridge to be firmer and to have higher stability; meanwhile, the pier manufactured by means of the single film growth craft has a hollow shape, thus ensuring that the manufacturing process of the electrical channel of the micro-bridge structure is more simple, without the need of tapping in the pier; only simple photoetching craft is needed to guarantee the connection between the electrode at the upper end and the electrode at the lower end.

本发明利用一次薄膜生长工艺完成桥墩、桥腿和桥面的支撑结构,大大降低了工艺难度和复杂性,且桥墩、桥腿和桥面三位一体,使得整个微桥的机械支撑更为牢固,稳定性更高;同时,本发明利用一次薄膜生长工艺制作出的桥墩成空心状,使得微桥结构的电学通道制作过程变得更为简单,无需另外在桥墩中开孔,只需简单的光刻工艺即可保证上端电极和下端电极相连。

By means of Hua, filial duty is assimilated into the ceremony of ancestor worship, enabling the ceremony to bear the function of filial education; by means of cultivation, the individual gains the filial concept and behavior; the blood ties will be sustained and the unity and identification of the family members will be strengthened in the way of converting filial duty into the love, the respectfulness, the support and the worship to parents and the activities of repairing the clan temple and continuing the family tree. Finally, filial duty coming into the different forms of reading, playing and writing scripture finishes the entire society\'s integration and sustains the social stability.4. It is because the Bai People group passes on the filial duty in the principle of Cheng that the filial duty still has an effect on the individual, the family and the society.

通过"化",孝道融入了祭祀祖先的仪式中,使该仪式具备了孝道教育的功能:通过"化",个体获得了孝道的观念和行为;将"孝道"化为对父母的爱、敬、养、祭,以及修祠、续谱的活动中,就可以稳固血缘家庭关系,增强宗族成员彼此之间的认同、团结和凝集力;最后,孝道"化"到莲池会的诵经、洞经会的谈经和圣谕堂的写经活动中,完成了对整个社会的整合,维持了社会的稳定。4、孝道之所以仍旧在白族的个体、家庭、社会中发挥作用,是因为他们在传承孝道时,本着"诚"的原则。

Firstly, by means of the man'-free-and-overall-development theory of the Maxthium , the author analyzes the Personal Literary Creation and confirms its value and significance as follow:the first,the emergence of the Personal Literary Creation means the vanishment of the laberal alienation ,ie,the personal authors' writings are not only the make-a-living means but the spontaneous displays of the human wills;the second, the Personal Literary Creation confirms "everyone' free development is the condition of all the people's progress",the human-liberating ideals of Marxism,and realises the all-round respects and concerns of the literature to human' diversity and peculiarity. Secondly, standing at the point of the contemprory Chinese history, it is right the individually depressed history in China that makes the Personal Literary Creation as the historical nacessarity. The emergence of the Personal Liberory Creation makes up to the limitation of the traditional public and representitive writing, and makes a great progress to diversifying the multi-cultural pattern in 90's of the last century, expanding the literatural domain, and displaying the complicated human natures.

本文首先运用马克思关于人全面自由发展的理论对私人化写作进行分析和观照,肯定其存在的价值和意义:一是私人化写作的到来意味着劳动异化的消除,作家的创作活动成为生命意志的自由表现,由手段上升为目的本身二是吻合了马克思人类解放的理想"每个人的自由发展是一切人自由发展的条件"实现了文学对人的丰富性、多样性、独特性的全面关怀与尊重;其次立足于当代中国的历史语境,它的出现弥补了一直以来公共化、代言式写作独占文坛的局限,在丰富九十年代的多元文化格局,拓展文学探索和表现丰富复杂的人性深度方面是一个很大的进步。

Kinds of Schiff base derivatives of 2-N-benzylidene Furanmethanamine have been designed. The Net Charges of main atoms, bond lengths, bond angles, dihedral angles, IR have been calculated by means of HyperChem6. 0 program. 36 kinds of Schiff base derivatives of benzylidene adenine have been designed. Also the net charges of main atoms, bond lengths, bond angles, dihedral angles, IR, the energy gap △E between HOMO and LUMO and the nonlinear second-order polarizability β have been calculated by means of HyperChem6. 0 program. The calculated results indicated that the nonlinear second-order polarizability β were up in accordance with the △E down.

设计了腺嘌呤缩苯甲醛36种衍生物,计算了这些分子的气相几何,腺嘌呤缩苯甲醛中各原子的电荷分布,最高占有轨道HOMO和最低未占有轨道LUMO的能量以及它们之间的能量差△E,同时还计算了这些分子的二阶非线性极化率β,我们发现腺嘌呤缩苯甲醛的二阶非线性光学系数与△E有近似一致的变化关系,随着△E的减小,二阶非线性光学系数有增大的趋势。

The synthetic study that makes theory into practice is little. And the dissertation of the strategy that base on the core capability is little too. By studying the characteristic of dynamic situation and core capability, the text formulizes the relationship between core capability and the strategy of the enterprises. By means of SWOT, the text takes the important position of time and core capability into account and tries to analyze core capability-based strategy. The text emphasizes the balance between the foundation of core capability and the exertion of core capability in order to acquire the dominance of competitive advantage. By means of PDCA, on the base of the dynamic characteristic of core capability and the strategy, the text integrates the core capability and the strategy together. On the base of the core capability, the text analyzes the strategy of low cost, difference, merger and diversification, and gets the goal and measurement of these kinds of strategy. Through the study of these issues, the text integrates the core capability and strategy, and.

文章从核心能力的特征入手,分析企业所面临的动态环境以及由此而产生的核心能力的动态性的特点,并阐述了核心能力动态性与企业战略之间的关系;本文运用SWOT方法,首次通过对时间因素与核心能、力的重要地位的融合,在基于核心能力的企业战略的分析方法方面进行了尝试性的论述;本文强调对企业的核心能力的构建与其运用进行合理的平衡,以保证企业资源的合理分配及更好的获得企业竞争优势:运用PDCA分析方法,在综合了核心能力与基于核心能力的战略的基础上,将两者综合起来进行动态的分析,力图实现核心能力与战略的更好的结合;另外,本文对传统的低成本与差异化战略进行基于核心能力基础上的分析,大胆提出了通过对核心能力及其要素分解的判断,进行低成本、差异化战略进行选取的方法,并将此方法与传统的低成本、差异化战略的决策模式进行优势比较;也通过与核心能力的结合,对企业购并和多元化战略进行新的角度的分析,得出了基于核心能力的企业购并与多元化战略的分析、制定方法以及实施的注意事项。

Tax evasion means that a taxpayer fails to pay or underpays the amount of tax payable by means of forging, altering, concealing, destroying without authorization accounting books, supporting vouchers for the accounts, by overstating expenses or not stating or understating income in accounting books, non-filing returns or filing false returns after notified by the tax department to report.

四、纳税人伪造、变造、隐匿、擅自销毁帐簿、记帐凭证,或者在帐簿上多列支出或者不列、少列收入,或者经税务机关通知申报而拒不申报或者进行虚假的纳税申报,不缴或者少缴应纳税款的,是偷税。

Co-firing characteristics of corn stalk and stone coal in different proportion were investigated by thermal gravimetric analysis. The results showed that corn stalk can improve the ignition and combustion of stone coal. The agglomeration experiments on corn stalk were made in a bench-scale bubbling fluidized bed with quartz sand as bed material and stone coal ash as additive. The results indicate that stone coal ash can inhibit agglomeration of bed material particles at temperature of 900℃. Agglomerate of blends was analyzed by means of scanning electron microscopy, energy-dispersive X-ray, and the bed materials were analyzed by means of X-ray fluorescence. Analysis showed that aluminum element and iron element in stone coal ash can react with alkali compounds and eutectics with low melting point, form a covering layer with high melting point on the surface of biomass and quart sand particles, thus inhibit the forming and transfer of low melting compounds.

以玉米秸秆与石煤按不同比例组成的混合物为研究对象,在TG-DTG热分析仪上进行了燃烧特性分析,结果表明玉米秸秆有利于石煤的着火和稳定燃烧,对石煤有一定的助燃作用;在小型鼓泡流化床实验装置上,以石英砂为床料、石煤灰为添加剂,进行了玉米秸秆成型燃料流化床燃烧的床料黏结实验,结果表明:石煤灰能够在生物质流态化燃烧过程中有效地抑制流化床床料黏结现象的发生;通过对实验中形成的结团进行扫描电子显微镜X射线能谱(scanning electron microscopy/Energy-dispersive X-ray- SEM/EDX),对床料进行X射线荧光光谱(X-ray fluorescence,XRF)分析,结果表明石煤灰中的Al和Fe能够与生物质灰中的碱金属化合物以及低熔点共熔物发生化学反应生成高熔点物质,并且覆盖在生物质碳颗粒与石英砂颗粒表面形成隔绝层,从而阻止低熔点物质的生成与迁移。

In order to obtain the fire parameters of cable tunnels and study the time-dependent and spacedependent distribution of the air temperatures and fire smokes inside the tunnels, cable tunnel model is established and fullscale fire simulation is made by means of FDS developed by NIST (National Institute of Standards and Technology). The distribution pattern of the smoke density, oxygen density and temperature are obtained by means of processing and analysis of the experiment data, so as to provide some effective methods for fire-fighting, rescuing and escaping. Furthermore, some suggestion is made on fire prevention.

为了获取电缆隧道火灾参数,研究隧道内空气温度及火灾烟雾浓度随时间、空间的分布情况,利用NIST (美国国家标准和技术研究院)研发软件FDS,建立电缆隧道模型,进行全尺寸火灾模拟,通过实验数据处理和分析,给出了电缆隧道发生火灾时烟气浓度和氧气浓度以及纵向温度的变化规律,为现实火灾救援灭火、人员逃亡提供一些有效方法,并对火灾预防提出一些建议。

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推荐网络例句

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。