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By using the Neighbor-joining method of Molecular Evolutionary Genetics Analysis sofrware, a dengrogram was obtained based on genetic distances. Another dendrogram was obtained by Maximum Likelihood methodv in PHYLIP (3.6) software. The bootstrap values vwere evaluated for each crunode of the dendrogram by means of bootstrap test.

利用分子进化遗传分析软件，采用邻结法构建系统发生树；同时根据等位基因频率，利用PHYLIP(3.6)分析软件，采用最大似然法构建系统发生树，应用自举检验估计系统树中结点的自引导值，并进行了系统发生分析。

PartⅡTransform the yeast, screen the excellent expression yeast strain, induce the yeast to express the fusion protein and depurate the target protein. The recombination plasmid was transformed into the yeast GS115 successfully by means of electrotransformation. The excellent yeast strain was got by G418 screening. We achived the yeast supernatant including target protein induced by methanol.

第二部分：酵母的转化、蛋白高表达菌株的筛选、重组蛋白的诱导表达及纯化实验利用电转化的方法，成功地将重组质粒pPIC9k-PTD-hA20导入毕赤酵母GS115，并通过G418浓度梯度筛选得到了蛋白高表达菌株，并对蛋白高表达菌株进行甲醇诱导表达，得到了含有目的蛋白的酵母上清液。

By means of automotive driving axle housing model tests, a weaken signal component covered by stronger exciting response signal was obtained by using of wavelet multiresolution analysis and a AR signal model was formed.

通过汽车驱动桥模型试验，利用子波变换多分辨分析技术，提取了淹没于响应信号中的微弱信号分量，并建立了裂纹信号的AR模型。

The model in this paper takes the form as belowThe main contents and results in this paper are as follows:In section 1, we state our main analytical results on equilibria of the above system. First, several sufficient conditions for coexistence of the steady-state are given by the standard fixed-point index theory in cone. Second, the global structure of the coexistence solutions and their local stability are established by using bifurcation theory. Third, the multiplicity, uniqueness and stability of positive steady-state solutions to this system are derived by means of perturbation theory of eigenvalues, standard regularity theory, Sobolev embedding theorem and fixed-point index theory.

本文主要有两章内容：第一章研究了该模型正平衡解的性质，可分为三部分：第一部分运用极值原理、上下解方法和锥映射不动点指标理论得到正平衡解存在的充分条件；第二部分利用分歧理论给出了平衡态系统正分歧解的结构，并讨论了局部分歧解的稳定性；第三部分利用特征值扰动理论、标准椭圆正则化理论、Sobolev嵌入定理及指标理论讨论了正平衡解的多重性、唯一性及稳定性，并讨论了参数对解的影响。

Methods The LPL cDNA was isolated from the human epiploon adipose tissue by means of RT-PCR. The LPL cDNA was ligated into the pcDNA3.1Zeo. The recombinant pcDNA3.1Zeo-LPL cDNA was identified by endonucleases, PCR and DNA sequencing. COS-1 cells were transfected with the recombinant LPL gene plasmid using Lipofectamine 20001M The LPL mass in cells and the culture medium were determined by a Markit-M LPL Kit. Spectrophotometry was used to measure the LPL activity.

采用RT-PCR法从人大网膜脂肪组织获取LPL cDNA基因，以pcDNA3.1Zeo质粒作为载体，运用基因克隆技术构建野生型人LPL cDNA重组质粒，限制性内切酶酶切、PCR以及双向测序鉴定重组质粒；运用Lipofectamine 2000将重组pcDNA3.1 Zeo-LPL cDNA质粒导入COS-1细胞，RT-PCR法检测LPL mRNA，ELISA法和比色法分别检测细胞裂解液及细胞培养基中LPL浓度及活性。

The erythritol is produced industrially by Zhongshi Gerui biotechnology Co., Ltd., Zibo by means of utilizing Candida lipolyti developed by China Food Fermentation Industry Inst. And with the preservation number of CGMCC No.1431 as the high erythritol yield spawn and deep liquid fermentation of glucose. The erythritol is used as functional sweetening agent in sugar substitute for diabetics, low calorie sugar, low calorie beverage, low calorie cake, low calorie candy, low calorie milk product, etc.

具体是指淄博中食歌瑞生物技术有限公司与开发出赤藓糖醇高产菌种――解脂假丝酵母 Candida lipolytic 的中国食品发酵工业研究院合作，利用保藏编号为 CGMCC No.1431的解脂假丝酵母 Candida lipolytica 菌种，用葡萄糖深层液态发酵，工业规模制取的赤藓糖醇；作为功能性甜味剂，在下述产品中的应用：糖尿病人代用糖，低热量佐餐用糖，低热量饮料，低热量糕点，低热量糖果，低热量奶制品和低热量冰激淋或低热量雪糕或低热量冰糕。

However, by taking advantage of the steric restriction offered by the three ethoxyl groups on the heads of the C8TES molecules, as well as the difference of the chemical activities between the OTS and C8TES molecules, it was possible to construct both the homogeneously and phase-separated mixed C8TES/OTS SAMs by means of the stepwise method.

但是利用C8TES分子头部三个乙氧基所形成的空间位阻以及C8TES和OTS分子之间反应活性的差异，采用分步法可以制备出混合均匀的均相和相分离(Phase-separated)的C8TES／OTS混合自组装单分子膜。

By making analyses,it is proved that the granules involved in rough set,quotient space and concept lattice can be described by abstract granules.Thus,the granules obtained by means of different ways achieve the unification,which will provide a way for formulization on granular computing.

通过分析处理，证明了粗糙集、商空间和概念格中出现的粒都可以采用粒空间中的抽象粒予以描述，使得以不同方式获取的粒得到了统一，从而为粒计算的形式化定义提供了思路。

Based on the Saint-venant equations describing the channel flow movement, the nonlinear algebraic equations derived by the use of Preissmann weighted implicit four-point scheme are solved with the Netwon-Raphson method. A combined Gauss main-element elimination method with compress-storage technique is proposed. The relaxation iterative method for solving branch river networks is extended, which can turn the problem of any complex river networks into the problem of a series of single rivers. The coupling of hydraulic model with water quality model is built up. The combined model was applied to a large complicated river networks with numerical hydraulic structure which influenced by strong tide, storm and heave rain. Visualization of computational results is realized by means of MapInfo, which displays the computational results in fast, convenient and accurate way.

水力模型以描述河道洪水波运动的Saint-Venant方程组为基础，用Newton-Raphson方法直接求解按Preissmann加权四点格式进行离散所得的非线性代数方程组，提出了压缩存贮形式的Gauss列主元消去法并对数值解的收敛性进行了讨论；将支流流量的松弛迭代方法从树状河系推广到了环状河网，使任意复杂河网水力计算的问题都可化为一系列的单一河道的水力计算问题；建立了水力模型与水质模型的联接，初步应用于既有洪水、暴雨、强潮等综合影响，又存在多种水工建筑物及水力调度问题的大型复杂河网中的非恒定流水力、水质计算问题；利用MapInfo对计算结果的可视化进行了研究，有利于决策者快速、方便、准确地作出决策。

Defatted wheat germ was micronized by comibmed method of hammer mill and colloid mill in order to increase the extraction rate of protein, followed by steps of alkali extraction and ultrafilter concentration, respectively. A quadratic mathematical model was developed statistically by means of Box-Behnken central composite design and response surface methodology.

为了提高麦胚蛋白的提取率，采用锤式粉碎与胶体粉碎相结合的方法对脱脂麦胚进行微细化处理，再用碱提、超滤浓缩法提取制备麦胚蛋白，同时，通过Box-Behnken中心组合设计及响应面分析，建立了预测麦胚蛋白提取率的二次多项数学模型，优化了蛋白质的提取工艺。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失