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However the three mentioned analysis methods has been so far assured only available to high pathogenic pathotype identification, which was probably associated with the high expression of gene related to high pathogenicity, on the other hand, pathogenicity differentiation of Cercospora zeae-maydis was probably controlled by some complex factors regulated by by polygenes.

相比较而言，只有强致病类型菌株利用三种方法进行鉴定有一定程度的相关性，这可能与强致病类型致病相关因子在蛋白质和DNA水平上的表达强度高有关，另一方面可能是有复合因子控制灰斑病菌的致病性分化，这恰好反映出该病菌的抗性遗传是由多基因控制的特点。

Guangdong Foretech Investment Consultants Co. Ltd （the qualified number: ZX0025）, was founded in August, 1995, is the one of the first security investment consultatory institutions that authenticated by Chinese Securities Regulatory Commission. Also, Foretech is the first company that was authorized to provide security investment consultatory services by adopting the quality control system-- ISO9001, and it is the only company approved by international professional identification in China so far. Foretech had been awarded "the national demonstrative company for quality, service and faith " in 2005,"the recognized database brand" in 2006,"AAA-class service quality corporation in the investment consultatory services industry" in 2006 and 2007,"the demonstrative company for customer service" in 2006 and 2007,"the prominent company for social responsibility" in 2006 and 2007, as well as "The top 100 good faith corportion in Guangzhou" in 2008, notably, this honor has been rewarded Foretech for 5 years consecutivly.

广东科德投资顾问有限公司成立于1995年8月，是首批经过中国证监会认证的证券投资咨询机构（资格证号：ZX0025）和国内首家通过ISO9001质量管理体系认证的具有证券投资咨询资格的公司，也是迄今为止国内唯一通过国际专业认证的证券咨询公司，并在2005年获得了&全国质量、服务、诚信示范单位&、2006年&中国名优数据库知名商标&、2006年&中国名优数据库优秀企业&、 2006年-2007年&全国证券投资咨询行业质量服务信誉AAA级单位&、&2006-2007年中国广州客户服务优质示范单位&、&2006-2007年广东省社会责任杰出贡献单位&、2008年&连续五年中国广州最具诚信度百强企业&等称誉。

In the rice leaves infected by RDV Yunnan isolate, the distribution of cells full of virus particles formed a belt which was a center of infection and multiplication of virus, around the cells fall of virus particles were some cells with the different extent of lesion as follows: disappearance of membrane system of chloroplasts, there were virus particles in some chloroplasts, and some sine or assembled virions in the mitochodria or cytoplasma but not in nuclei ,and intact subcellular structure in the cell far away from the infected center; In the rice leaves cell infected by Zhejiang isolate, some cells were full of virions, but the distribution of cells didn't form the belt, other cytopathological characters were similar to the cells infected by Yunnan isolate.

RDV云南分离株浸染的水稻叶片中，充满病毒粒体的细胞呈条带状分布，呈现出明显的侵染和增殖中心，在充满病毒粒体的细胞周围，相邻细胞有不同程度的病变，叶绿体膜系统消失，部分叶绿体中含有病毒颖粒，线粒体残体及细胞质中有分散或聚集的病毒粒体，细胞核中未见病毒粒体，离侵染中心较远的细胞中亚细胞结构较完整；RDV浙江分离株侵染的水稻叶片中，部分细胞充满病毒粒体，无明显的条带状分布，其他细胞病理特征与RDV云南分离株侵染的水稻叶片相似。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

The following are deemed the principal means:(1) To destroy radically by open persecution of the Church or by a hypocritical fraudulent system of separation between State and Church, all social influence of the Church and of religion, insidiously called "clericalism", and, as far as possible, to destroy the Church and all true, ie, superhuman religion, which is more than a vague cult of fatherland and of humanity;(2) To laicize, or secularize, by a likewise hypocritical fraudulent system of "unsectarianism", all public and private life and, above all, popular instruction and education.

以下是被视为主要是指：（ 1 ）破坏从根本上公开迫害教会或由一个虚伪欺诈系统之间的隔离状态和教会，所有的社会影响力的教会和宗教，阴险的所谓& clericalism &，并，尽量摧毁教会和所有真正的，即超人的宗教，这是多一个空泛的邪教的祖国和人类的；（ 2 ） laicize ，或secularize ，由一个同样的虚伪欺诈系统& unsectarianism &，在所有公共和私人生活，高于一切，通俗的指导和教育。

The following are deemed the principal means:(1) To destroy radically by open persecution of the Church or by a hypocritical fraudulent system of separation between State and Church, all social influence of the Church and of religion, insidiously called "clericalism", and, as far as possible, to destroy the Church and all true, ie, superhuman religion, which is more than a vague cult of fatherland and of humanity;(2) To laicize, or secularize, by a likewise hypocritical fraudulent system of "unsectarianism", all public and private life and, above all, popular instruction and education.

以下是被认为主要是指：（ 1 ）为了从根本上破坏了公开迫害的教会，或由一个虚伪欺诈系统之间的隔离国家和教会，所有的社会影响力的教会和宗教，不知不觉地称为&教权主义&，并，尽可能地摧毁教会和所有真正的，即超人的宗教，这是一个多含糊邪教的祖国和人类；（ 2 ） laicize ，或还俗，由一个同样的虚伪欺诈系统& unsectarianism &，所有公共和私人生活，尤其是流行的教学和教育。

Liu Wen-hsiung said that at present market sales by Pearl Ma Pui by breeding output, quality is far better produced by Pinctada maxima.

刘文雄说，目前市场上所销售的珍珠多由养殖马氏贝产出，质量远不如大珠母贝所产。

So far, more than 400 people to the United States, Canada, New Zealand, Saudi Arabia, working in 13 countries, nearly 1,800 people to Beijing, Shenzhen, Tianjin, Shanghai and other mainland city of employment, the employment rate reached 100 percent, and the quality of employment and stability of the good; Since 2003, the schools are with the United States, Canada, Australia, New Zealand and four countries, five institutions have established stable cooperative relationship, the external output channels, step by step flow; Ministry of Education, Ministry of Health and Hebei Province, Chengde City, as well as foreign institutions in the schools to set up various training bases 5; the past five years, the school sponsoring scale nearly one-fold, the campus covers an area of increased 113%, construction area increased by 1.5 million square meters, laboratory instruments and audio-visual equipment , books and other school conditions have been substantially improved; the establishment of teaching practice base 95, including major hospitals outside the province such as the Beijing Union Medical College Hospital, PLA General Hospital, the Third Affiliated Hospital of Peking University Department of Medicine, Shenzhen Second People's Hospital, Shanghai Punan A total of 44 hospitals, etc.; the overall quality of teaching staff has been significantly improved, train 21 graduate students, teachers, served as the textbook editor of the National Commission 8, deputy editor of 3; In recent years, the school received more than 20 each year and several other universities abroad, missions or study visits of experts and scholars, the social impact of growing; school sponsoring the good results achieved by the media attention, the China Education reported that vocational and technical education in China magazine, China's economic construction magazine, Hebei Daily News and a number of media school characteristics school carried out varying degrees of coverage, such as the China Education Daily,"features professional make angels in white taken the door," the Chinese Journal of Vocational and Technical Education "angel from here into the world," and so on.

截至目前，已有400多人到美国、加拿大、新西兰、沙特阿拉伯等13个国家工作，有近1800人到北京、深圳、天津、上海等国内大城市就业，就业率达到了百分之百，而且就业质量和稳定性良好；2003年以来，学校分别与美国、加拿大、澳大利亚、新西兰等4个国家的5个机构建立了比较稳定的合作关系，对外输出渠道逐步畅通；教育部、卫生部和河北省、承德市以及国外机构在学校建立各类培训基地5个；近5年来，学校办学规模扩大了近1倍，校园占地增加了113％，建筑面积增加了1.5万平方米，实验仪器和电教设备、图书等办学条件得到了实质性的改善；建立教学实习基地95个，其中省外大医院如北京协和医院、解放军总医院、北京大学医学部第三附属医院、深圳第二人民医院、上海浦南医院等共44所；师资队伍综合素质得到显著提高，培养研究生21名，教师担任全国统编教材主编8部，副主编3部；近年来，学校每年接待20余所兄弟院校和数个国外访问团或专家学者的考察访问，社会影响不断扩大；学校办学取得的良好成绩受到了媒体的关注，中国教育报、中国职业技术教育杂志、中国经济建设杂志、河北日报等多家媒体对学校特色办学情况进行了不同程度的报道，如中国教育报的《特色专业让白衣天使跨出国门》、中国职业技术教育杂志的《天使从这里走向世界》，等等。

Far from following the PA's script, he began to lambast Egypt for pleasing Israel by building siege walls around the Gaza Strip, the enclave run by Hamas to the exclusion of Fatah, its secular-minded rival headed by the PA's president, Mahmoud Abbas.

他从基本做起并在巴勒斯坦权力机构站住了跟脚。为了讨好加沙地带他四处奔波，哈马斯飞地来说，对法塔赫排除以色列围困埃及十分排斥，约旦河西岸的世俗统治者巴勒斯坦权力机构的权力机构主席阿巴斯领导。

I know," wrote Hitler,"that one is able to win people far more by the spoken than by the written word...." Later he adds contemptuously:"For let it be said to all knights of the pen and to all the political dandies, especially of today: the greatest changes in this world have never been brought about by a goose quill!

希特勒写道：&我知道，一个人通过演说能争取到的人比书面文字多得多……&以后他又傲慢地补充道：让我们告诉所有的文人和政客，特别是今日的文人和政客：世上最大的变革从来不是由鹅毛笔引起的！

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。