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RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The formula was established about calculating design parameters and technological parameters of ideal strip and bent strip in the period of forming. The thesis presented how to calculate the deformed area length and contact are radius of contact deforming between two elastic bodies on condition of the friction. The calculating formula of pinch force about plate-strip pinch mill was given and the results were analyzed that friction coefficient and radius of pinch rolls are very important for determining pinch force. Moreover, the rule of deforming and stress distribution of different 〓 ratio were gained on condition that the deforming was considered to be non-uniform distribution in the gap. The differential equations were got that calculate the displacements of large deflection of elastic cantilever rectangle plate with fixed end and the formula of the Finite Strip Method was conducted to study the regularity of mid-plane displacements, deformation and stresses for elastic-plastic large deflection bend of cantilever parallelogram plate with fixed or slidable end by integrating Prandtl-Reuss equation.

建立了一套完整的设计参数计算公式和在成型过程中理想带钢或具有月牙弯带钢的工艺参数的调整公式；在考虑摩擦力的条件下，对两弹性体接触变形过程中的接触变形区长度和接触弧半径用位移解法进行了研究，推导了板带递送机的递送力计算公式，从而得知在递送机设计过程中，应选择适当摩擦系数和辊身直径；另外，还从辊缝内带钢实际变形出发，分析了对于不同的〓值，带钢的变形规律及应力分布状况；建立了固定端悬臂矩形板弹性大挠度弯曲变形的控制方程，并且在对Prandtl-Reuss方程的积分处理的基础上，导出了固定端和滑动端悬臂平行四边形薄板的弹塑性大挠度的中面伸展位移、变形和应力的有限条元法公式，编写了Fortran语言计算程序，在各种参数条件下，对螺旋焊管成型过程变形规律进行了全面的分析研究，从而为螺旋焊管的生产和进一步的改进提供了理论依据。

In this paper, according to the theory of electromagnetic fields, firstly, electromagnetic field of large and medium asynchronous motor is calculated from the angle of coupled fields, secondly, the end voltage of the stator is taken as constraint condition and starting current in stator is iterated in the numeral computation process, finally starting current and starting torque at rated voltage are gained, and by comparing calculation results with test results ,starting performance of large and medium asynchronous motor meet the demand of project practice.

本文根据电磁场理论，从耦合场的弱耦合角度首先计算了大中型异步电动机的电磁场，在电磁场的数值计算过程中，以定子端电压为约束条件，对定子起动电流进行了迭代计算，得到了额定电压下的起动电流和起动转矩，并把正弦时变场计算结果与实测结果进行对比，表明大中型异步电动机的起动性能的计算结果满足工程实际的需要。

The best ink in the off-time after opening the packaging end use, not to open the ink on the outside when not in use Add to carton packaging, the cartridge so as to avoid talking about the dust falling into the hands of the next use of the dust caused by plugging into the nozzle first, the head following a reset state of ink wash sponge pad, open the front cover, press the Print Paper knife, move out, the hair can not suction clean cloth towel or sponge the ink pad, a large amount of about 10 days, then wash first, because each sponge pad is printer ink wash drawing of fatigue, if the dust and fatigue more ink will be solidified with the protection of Garra and print images will be the first flush, if the plug was found, and if the use of cleaning 3 seconds is not normal use, we must enter the printer maintenance menu kk2 or use kk1 cleaning, large format printers and a small printer operation principle is similar, if the correct maintenance and use, it will increase the service life, which Like is like a car, but also for us to maintain and are familiar with it

墨水最好在拆开包装后断时间内使用完，不要把打开的墨水放在外面，不用时包装好放入纸盒内，以免墨盒嘴上落入灰尘，下次使用时灰尘进入喷头造成堵头情况，喷头复位状态下面有一个洗墨海绵垫，打开前盖，按下打印切纸刀，往外移，用不起毛的纸巾或布吸干净海绵垫的墨水，用量大的话大约在10天清洗一次，因为海绵垫是打印机每次冲洗时吸取疲墨水的，如果灰尘多就会和疲墨水凝固在一起，对墨头的保护和打印头冲洗会有影像，如果发现堵头情况，如果使用清洗3秒还不能正常使用，我们要进入打印机的维护菜单用kk2或kk1清洗，大幅面打印机和小打印机的操作原理是差不多的，如果正确的维护和使用，它的使用寿命就会增长，这也好比一辆轿车一样，还要我们去维护和熟悉它

This invention relates to one large size space coordinate measurement technique and device based on automatic visual guidance, which aims to provide one coordinate measurement and device and adopts the following scheme: altometer fulfills the points to be tested for automatic identification and measurement through variable cameral guidance to realize the large size space coordinate identification and fine measurement by each crossing constraints.

本发明的目的是提供一种基于自动视觉引导的大尺寸空间坐标测量方法及检测装置，该装置具有测量速度快、非接触、能在恶劣的环境中使用、较高的测量重复性特点，本发明采用的技术方案是，经纬仪通过可变焦摄像机的引导完成对被测点的自动识别及测量，由各经纬仪间的交汇约束实现大尺寸内空间坐标的自动识别及精密测量。

A tunneling machine for producing large tunnels in soft rock or wet, clayey, unconsolidated or bouldery earth by simultaneously detaching the tunnel core by thermal melting a boundary kerf into the tunnel face and forming a supporting excavation wall liner by deflecting the molten materials against the excavation walls to provide, when solidified, a continuous wall supporting liner, and detaching the tunnel face circumscribed by the kerf with powered mechanical earth detachment means and in which the heat required for melting the kerf and liner material is provided by a compact nuclear reactor.

&…一个在软岩石，或湿的、粘土状的、疏松的，或巨砾泥土中挖掘隧道的隧洞掘进机，用先进的以上升暖气流进行隧道核心分离，将边界切口熔入隧道面，同时通过使偏倚在隧道壁上的熔融物料在凝固时提供一个连续墙来支持衬垫，形成一个支持挖掘的墙壁；破碎在切口周围划了界限的隧道核心-用有动力的机械土地分离的方法，在隧道核心中，需要如此操作的热力以及衬垫物料由一个小型的核反应堆提供……&

The main characteristics include: the quiet Sun corona has a rapid response to the flare heating, within 2 second the corona temperature increases nearly one order of magnitude, but later such increase becomes very slowly; the downward velocity at early stage of impulsive phase is too small to be negnected; chromospheric evaporation made by conduction shows some intensely, the maximum evaporated velocity may be as large as 1000 km/s; the chromospheric condensation appears just when the chromospheric evaporation takes place and its place always coincide with that of transition region in the early stage; the maximum of chromospheric condensation occurs also in the early stage of impulsive phase, at which the density in condensation may be larger two order of magnitude than that of surround and the downward velocity may be as large as 100 km/s; after the maximum of chromospheric condensation, its strongth decreases gradually but its width becomes greater with time and have a tendency to transport into deep chromosphere; the front of chromospheric condensation heats atmosphere obviously and the temperature in the chromospheric condensation is higher than the original case; the downware velocity of transition region may be greater than 100 km/s at the early stage; when the evaporated material collide at the top of loop, the densityand the presure there increase rapidly, which result in the formation of a shock wave; and so on.

在改善色球辐射损失和精确分辨过渡区的基础上，通过联立求解连续性方程、动量方程和能量方程，构造了一维磁限制性耀斑环在热沉积作用下的大气动力学模型，获得了耀斑热模型下脉冲相早期较为细致的大气演化理论图象，其主要特征是：耀斑对宁静日冕的加热相当迅速，仅2秒钟日冕温度增加即达一个量级，之后日冕温度增加缓慢；脉冲相早期日冕向下速度很小，几乎可以忽略，热传导驱动色球蒸发，其过程较为激烈，蒸发极大速度可达到1000km/s；色球压缩区与色球蒸发几乎同时形成，且在早期其位置总是与过渡区位置重合；色球压缩极大发生在脉冲相早期，其时密度可高于周围约2个量级，向下速度可超过100km/s；色球压缩极大后，其强度逐渐减弱，但宽度明显增大，且渐渐脱离过渡区往色球传播；压缩区内物质温度要高于原先的大气温度；过渡区下降在脉冲相早期相当迅速，可超过100km/s；当蒸发物质在环顶高速相碰时，环顶的密度、压力迅速增大，且温度也有所增加，导致形成激波，其波面以每秒上百公里的速度自环顶向下传播；在脉冲相前期，日冕软x辐射对色球的加热作用可以忽略；等。

And the frontal technique is adapted to solve the FE equations. The techniques are studied to deal with the large slip frictional contactproblems between a deflectable body with large deformation and a rigid bodywith flat or curved surface. A new algorithm is presented to resolve such contactproblems, including incremental displacement constraints and force constraints,and the modules are programmed. The performances of tire under static load invertical loading state and camber state are analyzed by this method. And theexperiments were carried out at the same condition as used in analysis process.

研究了大变形柔性体与具有平面及曲面外形刚性体之间的大滑动摩擦接触问题，提出了求解这类接触问题的位移增量约束和主动力增量约束处理方法；编制了大变形柔性体与具有平面及曲面外形刚性体之间的大滑动摩擦接触问题求解模块；利用本文提出的大滑移接触问题处理方法，分析了轮胎在径向静载和静态外倾加载条件下受力和变形，并对部分分析结果进行了试验验证，验证结果表明，本文提出的位移增量约束和主动力增量约束处理接触问题的方法是正确可靠的，而且具有精度高、收敛性好、收敛速度快的优点。

Methods A wet hip joint's sample from corpse was scaned by CT and all images of every cross-section hip joint's layer was obtained, and an hip joint's model was contructed with Unigraphics NX 2.0 and Solid Works 2006 SP 0.0 software. The models were assembled when simulated total hip arthroplasty after total hip joint prosthetic and ischiofemoral ligament model had been constructured. Analysis was done on von Mises stress distribution and amount of total hip component when simulated seated leg-crossing manuver. The bone and metal components were meshed as rigid bodies, composed of three-dimensional, all-quadrilateral rigid body elements, and hip joint capsule ligament was fully three-dimensional, hexahedral continuum elements representations. Results The current form of this ischiofemoral ligament's reconstruction finite element model provided for multi-body contact, large displacement interfacial sliding, large deformation capsule ligament representation, and clearly reflected the real ischiofemoral ligansent's anatomy and biomechanical behavior. The maximum on Mises stresses at the joint interface was more than that fur an otherwise identical hardware-only construct.

选择成人尸体髋关节行CT成像得到髋关节每层横截面图像，提取边界坐标，利用有限元分析软件Unigraphics NX 2.0和Solid-Works 2006 SP0.0构建出髋关节三维有限元模型及人工髋关节假体，并模拟全髋置换术进行装配，将坐股韧带按其术中重建位置进行构建骨骼与金属模型采用三维十节点四面体实体单元进行网格划分，关节囊六面体连接单元划分，模拟坐位腿交叉动作载荷获取假体撞击过程的角活动度和相应的von Mises应力分布情况结果所构建的多体连接、大界面划移、大变形的坐股韧带重建有限元模型，客观反映坐股韧带真实解剖形态及生物力学行为，增加坐股韧带重建的模型其关节面的最大主应力值大于仅有金属的模型。

And more recently, it has been working in monocularly camera system have developed out of the interchangeable lens type digital camera, a journalist or professional photographer, because the old cameras equipped with and it is compatible with the camera, but in monocularly motorsied and rich control in place, and to use this old advanced in monocularly camera, the only disadvantage is that there is still a lens multiplication problem, to the wide angle shooting embitterthe, but improvement of aircraft and will soon be listed but by large camera with digital back systems are commercial photography only because the file is large, and you can control the level of the grass, and so on up-dip perspective, but price and a gateway to nearly one million, and the economy.

而最不远由老牌不复眼相机编制所成长不入的可互换镜头式数字相机，是故事记者或副业影相师的最爱，因为旧有镜头等配备和其编制兼容，而不复眼相机的机动性与丰裕的操控功用包罗万象，和搁置原有矮档不复眼相机实在一模差别，独一弊端是仍有镜头倍率的题目，给广角拍摄时甚为悲苦，但保守机种很快不离会上市！而由不小型相机配上数字机不面的编制是贸易影相的钝器，因为其档案较不小，又可把持仰俯等透视角度，但代价不远百不一，经济门坎较矮。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。