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The full stress optimizes the application in connecting a design of greenhouse with; Even the experimental study of the only warm environment of winter of a plastic greenhouse; Intensify the current situations and trends of raising pig"s craft development; The scale beasts and birds cultivate the technical research progress of the environmental project; Effective way utilized in recycle treatment of the beasts and birds offal; The nourishing oral liquid culture current situation of Japan and new technology; Engineering of animal husbandry of our country and industrialized development are 20 years; Technological economic analysis that heat energy retrieves in winter of chicken coop of North China; Connect large-scaly with the design and test of the natural day-lighting of a chicken coop; Even the experiment research of the environment of summer of a chicken coop; The pig house cools the current situation of technological application study in summer; Application and design of the vertical ventilating system of pig house; The evaporation of agricultural building cools the current situation and prospect of technological study and application; Of our country the South open to cool the question summer in chicken coop; Vertical air current organization that ventilated of chicken coop; Innovation and Development of China"s Animal Husbandry project Science and Technology; The pig farm of scale is raised in the shed and raised the clean production technology mode scattered to study; the Project of the cow is raised in the shed in the scattered fence; Give up and raise sheeps new craft mode to visit for the first time in scale; The piglet keeps the case warm one degree of characteristics of playground of body temperature is studied; Structure optimization of the joining type pig house of roof truss of light steel; Lose electrical power such nearly one hundred as the impact on airtight type hot wet environment of pig house ,etc.

满应力优化在连栋温室设计中的应用；连栋塑料温室冬季光温环境的试验研究；集约化养猪工艺发展的现状与趋势；规模化畜禽养殖环境工程技术研究进展；畜禽废弃物的资源化利用的有效途径；日本的营养液栽培现状及其新技术；我国畜牧工程技术与产业化发展20年；华北地区鸡舍冬季热能回收的技术经济分析；大型连栋鸡舍自然采光的设计与测试；连栋鸡舍夏季环境的实验研究；猪舍夏季降温技术应用研究现状；猪舍纵向通风系统的应用与设计；农业建筑蒸发降温技术研究与应用的现状与展望；我国南方开放式鸡舍的夏季降温问题；鸡舍纵向通风的气流组织；中国畜牧工程科学技术的创新与发展；规模化猪场舍饲散养清洁生产工艺模式研究；奶牛舍饲散栏饲养工程工艺研究；规模化舍饲养羊新工艺模式初探；仔猪保温箱体温度场特性研究；拱型轻钢屋架猪舍的结构优化；夏季停电对密闭式猪舍热湿环境的影响等近百篇。专著与教材：《家畜环境与设施》《畜牧工程——科技创新与发展》《可食用观赏植物》《园艺设施学》《林果花菜设施快速栽培技术》《农业生物环境与能源工程》《居室花卉装饰》《现代温室技术》《中国养鸡学》等。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Therefore it's necessary to contol the concentration of TW80 to use it in the remediation of pyrene contaminated soil by AMF and corn integrated systerm.4. The AMF community and the single species derived from rock oil-contaminated soil all can be combined with corn and promote the remidation of pyrene contaminated soil , the original AMF community has obvious advantages and behave best.The residual pyrene concentration of soil in the treatments with the mixed AMF community M4 (Glomus intraradices, Glomus mosseae, Glomus etunicatum and Acaulospora tuberculata) and corn integrated systerm got 0.12mg/kg,0.54mg/kg and 3.92mg/kg respectively in 1mg/kg, 10mg/kg and 50 mg/kg pyrene treatments,decreased 32.6%,58.6% and 42.2% respectively than that of the treatment of M1,decreased 28.1%,59.9% and 36.6% than that of M2(G.intradices), decreased 2.7%,55% and 23.5% than that of M3 (G.intradices + G.mossea).5.Large scale reproduce overall of AMF community which coming from rud oil-contaminated soil in the absence of the original conditions will change the quantiy,composition and structure of the AMF population and may has negative impact on it's function in the remedition of soil pyrene contamination.

因此，在植物与AMF联合修复土壤芘污染中使用TW80应该注意控制TW80的浓度。4、从石油污染土壤中扩繁出的AMF混合菌群及其中的单一菌种与玉米联合均能促进土壤芘污染的修复，原始混合菌群具有明显的种群优势，其与玉米联合修复土壤芘污染的效果最佳。1mg/kg、10mg/kg和50mg/kg芘处理土壤中，玉米接种混合菌种M4(Glomus intraradices， Glomus mosseae， Glomus etunicatum和Arcaulospora tuberculata)处理土壤的芘残留浓度分别为0.12mg/kg，0.54mg/kg和3.92mg/kg，分别比接种单一菌种M1处理降低了32.6%，58.6%和42.2%，比接种单一菌种M2(G.intradices)处理降低了28.1%，59.9%和36.6%，比接种复合菌种M3(G.intradices+G.mossea)处理降低了2.7%，55%和23.5%。5、从石油污染土壤中扩繁出的混合AMF菌群在无原始条件下的整体扩繁会改变菌群的组成及其结构，并会对菌群与玉米联合修复土壤芘污染的功能产生不利影响。

Due to food pressure, grain product in the western Jilin Province will be enhanced in the near future. Farmland areas in the area will be decreased slowly in the near future, but with the technique development of irrigation establishment, paddy areas and utilization amount of fertilizer and pesticides will be increased rapidly. So, the NPS pollution from farmland will be more serious. The main lakes in the areas such as Moon Lake and Qagan Lake have been polluted by N and P, and the content of salt in the Nenjiang River has been increasing since last 30 years. All of these show that the negative effects of NPS pollution in the area have been serious and should not be neglected by the scientific research and corresponding government management. To deal with the contradiction of agriculture development and water pollution, the trend of increasing paddy area and using amount of fertilizer, pesticide and herbcide in the western Jilin Province should be controlled. Also, the drainage of return flow from paddy should be controlled and return flow should be disposed with ecotechniques by use of the large areas of lakes and mires in the area. Through these measures, the quality of surface water could be protected and the degeneration of ecoenvironment in the area could be contained.

我国由于粮食压力，未来吉林西部的粮食生产还将进一步加强，随着种植业结构的调整，垦殖率会略有下降，但随灌溉设施的建设与完善，水田面积将进一步扩大，化肥、农药的用量还将快速增加，农业活动引起的非点源污染将更加突出，而研究区的主要湖泊月亮湖、查干湖目前已达中等富营氧化水平，嫩江水质的咸化趋势明显，因此农田非点源污染物的流失及其内流区外流化面积的增加对地表水体富营养化、咸化的作用愈来愈明显，农田非点源对地表水水质的影响已不容忽视，应引起社会的关注，为协调农业发展与水污染这一矛盾，吉林西部应遏制化肥、农药施用量增长过快和水田过度开发的势头，控制洗盐回归水的排放量，利用区内广泛分布的大量泡沼对农田径流和回归水进行生态处理，既保护了地表水的水质，又有利于生态环境的改善。

The company is teamed up with medium and high class technical and management personnel experienced in business and management,including nearly 20 grade 1 constructors and grade 1 project managers,lt has rich construction experiences in high building and steel-structured domestic architecture,large industrial workshop,waterworks,crane and nonstandard equipment,With sound regulations,and complete and strict quality and OHS assurance systems,the company keeps in line with national and local decrees and legislations and carries out scientific,regular,routinized and humanbased project management.

公司由一大批&精业务、善管理&的中、高级专业技术、管理人员组成的队伍，其中一级建造师和一级项目经理近20名。有严谨的高层建筑、钢结构民用住宅建筑、大型工业厂房、水工工程、起重机及非标设备的施工经验。公司有一整套健全的规章制度，有完整严密的质量、安全、职业健康环境保证体系。公司严格执行国家和地方的法令、法规、对项目实施科学化、规范化、程序化、人性化的全过程管理。

Major process products have: jig and fixture of aircraft assembly plant and auxiliary tools; tire vulcanization and tire production line、transmission line 、loader、Lay-tire equipment、tire mould、clean mould machine、repair mould machine、tire mold 3D intelligent;warehouse palletizer;oil platform;fur machinery; electrical engineering alloy、contact process;hydraulic elevator accumulator、1t—50t electric hoist and crane machine; door and window manufacturing machine、varouris kinds of large scalestabilization machinery and mixing machine; Grain and oil produce equipment、auxiliary machine and control system; Satellite Launching base facilities; tire forming machine、cutter etc equipment parts; various kinds of box of reducer; milk packing machine and parts; elevator up and down operate panel、controlling Single Chip Micr-computer and Elevator Group Supervisory Control System; repair circuit disk produce equipment and imported equipment copped; air and water treatment equipments,etc.

主要加工产品有：飞机组装厂夹具和辅助机具；轮胎硫化机及轮胎生产线、传输线、抓胎机、存胎器、轮胎模具、洗模机、修模机、轮胎模具智能三维检测仪；立体仓库堆垛机；石油钻井平台；皮毛机械；电工合金、触头加工；液压升降台、1吨－50吨电葫芦和起重机；门窗生产机具、各种大型搅拌机械和研磨机械；粮油生产设备、辅助设备和控制系统；卫星发射基地设施；轮胎成型机、裁断机等设备配件；各类减速机箱体；牛奶包装机及配件；电梯升降操纵面板、控制单片机和电梯群控系统总成；线路芯片生产设备大修和进口设备仿制；空气和污水处理设备等。

Animation Department adhere to universities and research institutes to achieve the teaching, research, talent, a perfect combination of the results established animation industry, from the early creative planning, medium-term production, post-processing, marketing, product development, in areas such as derivatives has a relatively complete The industrial chain support in this regard thanks to animation department teaching, the other hand, benefited from the cooperation with other colleges and support: First, College of Humanities and Arts have a strong literary team, there can be for manner mining history, culture and local culture to carry out works of literature, script writing, animation and shooting script creation; second, College of the Arts Academy of Fine Arts, the Television Animation Art Institute of professional experts and professors, teachers and students can focus on the requirements of dynamic animation creation diffuse image design, animation production, post-processing and other special effects; third School of Journalism, Art Institute inherent in the industry and market channels, you can promote products in the market for the animation to provide support; Fourth, colleges and Arts and Crafts Arts and Crafts Association, a large number of member companies may be based on mature animation image processing technology toys and other derivative products.

动画系坚持产学研一体化，实现了教学、科研、出人才、出成果完美结合，建立了动漫产业从前期创意策划、中期制作、后期加工、市场推广、衍生产品开发等各方面具有相对完整的产业链条支撑，这一方面得益于动画系的教学成果，另一方面也得益于与其他学院的合作与支持：一是人文学院和艺术学院均有强大的文学创作班底，可以有针对性地挖掘历史文化和地方文化，进行动漫作品文学剧本创作和分镜头脚本创作；二是艺术学院、工艺美术学院、影视艺术学院动画专业的专家教授和师生，可针对动漫创作要求进行动漫形象设计、动画制作、后期特技加工等；三是新闻学院、艺术学院固有的行业和市场渠道，可以为动漫产品的市场推介提供支持；四是工艺美术学院和工艺美术协会众多的会员企业，可以根据成熟的动漫形象加工生产玩具等衍生工艺产品。

Here Pilgrims roam, that stray'd so farr to seek In Golgotha him dead, who lives in Heav'n; And they who to be sure of Paradise Dying put on the weeds of Dominic, Or in Franciscan think to pass disguis'd; [ 480 ] They pass the Planets seven, and pass the fixt, And that Crystalline Sphear whose ballance weighs The Trepidation talkt, and that first mov'd; And now Saint Peter at Heav'ns Wicket seems To wait them with his Keys, and now at foot [ 485 ] Of Heav'ns ascent they lift thir Feet, when loe A violent cross wind from either Coast Blows them transverse ten thousand Leagues awry Into the devious Air; then might ye see Cowles, Hoods and Habits with thir wearers tost [ 490 ] And flutterd into Raggs, then Reliques, Beads, Indulgences, Dispenses, Pardons, Bulls, The sport of Winds: all these upwhirld aloft Fly o're the backside of the World farr off Into a Limbo large and broad, since calld [ 495 ] The Paradise of Fools, to few unknown Long after, now unpeopl'd, and untrod; All this dark Globe the Fiend found as he pass'd, And long he wanderd, till at last a gleame Of dawning light turnd thither-ward in haste [ 500 ] His travell'd steps; farr distant he descries Ascending by degrees magnificent Up to the wall of Heaven a Structure high, At top whereof, but farr more rich appeer'd The work as of a Kingly Palace Gate [ 505 ] With Frontispice of Diamond and Gold Imbellisht, thick with sparkling orient Gemmes The Portal shon, inimitable on Earth By Model, or by shading Pencil drawn.

此外还有很多，说起来未免太冗长，他们无非是些未成熟的、痴呆的、穿着黑、白、灰色衣的身带骗人的假法宝的隐士和托钵僧。其中有的在这里云游巡礼，曾到各各他去寻觅那活在天上的死人，临终穿上圣多明我派或圣方济派的袈裟，以证明自己确实去过极乐天堂，他们误以为这样打扮就可畅通无阻。他们通过七星天，通过"恒星天"，通过那权衡黄道振动均势的"水晶天"，并且还通过"原动天"。圣彼得手拿钥匙，站在天堂的边门口，像是在等待他们，正要举步登上天堂的阶梯时，看吧！从左右吹来一阵猛烈的横风，他们被斜吹到十万里外的远空中去：那时看见僧帽、头巾、袈裟，连同它们的穿戴者一起被吹翻扯烂，还有圣骨、念珠、免罪券、特免证、赦罪证、训谕，全被高高卷起，都成了风的玩具，他们飘过这世界的背面而远落在广大的地狱边缘，被称"愚人的乐园"，很久之后将是家喻户晓的，但是现在却是人迹罕至的地方。魔王走过这整个黑球，经过长久的漫游，终于发现一线曙光，便加紧他的步伐，向那边走去。他远远望见一座高大的建筑，从它那宽大的阶梯拾级而上，可以抵达天国的城垣，它的顶上，像有比王宫还要富丽的宫门，它的正面镶有金刚石和黄金；大门上密饰着东方的珠宝，光彩夺目，人间的浓淡画笔也无法描绘它。

Finally, it analyses the time complexity of the algorithm and researches how it is influenced by culture operator.3、The paper presents the evaluation standard of the GA's application capability. Basing on characters such as continuity, multi-peak, vibration, randomicity as well as large-scale, five functions are selected to test search ability and robustcity of co-evolution algorithm. Finally, it analyses the simulation result and researches the influence of algorithm brought by culture operators.4、Basing on the concept of collection overcast, it researches the task distribution issue and constitutes delaminated math model on task distribution issue. It puts out the co-evolution algorithm of subtask's decomposing. The experiment compare IGA、SGA to CN and validates the efficiency of co-evolution algorithm on the NP completeness issue.5、Being aim at the optimization issue of load of antenna near ground, it combines many GA strategies and puts forward strategic meme. And it puts out co-evolution algorithm of load of antenna design. And it emulates the optimization design of load of antenna near ground. Finally, it valuates the co-evolution algorithm's efficiency on the continuum search issues of multi-variable and multi-peak value.6、Being aiming at the knowledge of image model matching, it adopts single meme and real code. It puts out fast co-evolution matching algorithm strategy. Basing on NPROD resemble measurement, I emulate the indiscrimination model matching and discrimination model matching. Finally, it valuates the co-evolution algorithm's efficiency on the real code and real time search issue.

分析了算法的时间复杂度，研究了文化算子对算法时间复杂度的影响。3、提出了GA的应用性能评价标准，从连续性、多峰性、随机性、振荡性、广域性多个角度出发选择五个测试函数，对共同进化算法的搜索性能和鲁棒性作了函数优化的性能测试，分析了仿真结果，研究了文化算子对算法的影响。4、基于集覆盖的概念，研究了MAS中的任务分配问题，建立了任务分配问题的分层数学模型，给出了子任务分解共同进化算法，实验比较了IGA、SGA、CN，验证了共同进化算法对NP完全问题的有效性。5、针对近地天线加载优化设计问题，结合多种改进GA策略，提出了策略型拟子，给出天线加载设计的共同进化算法，对有耗半空间对称偶极子天线加载优化设计作了仿真实验，验证了共同进化算法对多变量多峰连续搜索问题上的有效性。6、针对图像模板匹配问题的领域知识，采用单类拟子和实数编码，给出快速共同进化匹配算法策略，基于NPROD相似度测度，仿真试验了无差别和有差别模板匹配，验证了共同进化算法对实数编码和实时性搜索问题上的有效性。

In this paper we build a university scientific research management information system based on Client/Server and three-layer structure of ASP technology, and introduced the design aims, tasks and principle of the system with systematic investigation and analyzsis of demand., With the principle of integrality, practicability, opening, precursive and generality, data stream and data structure are analyzed detailedly, and logistic model which fits university scientific research management was established based on advanced structure system analysis method. In addition, it puts forward a series of the whole scheme of scientific research management system which bases on internet adapts large database development.the design of this system adapts new internet/intranet techonlogy and it can make us gather and share the whole provincial university scientific research information.lt studies and establishes oology structure and using platform of our provincial university scientific research management system, In addition,it also designs and formulates the system at the experimental unit(the scientific research department of NEAU).The scientific research management information system of NEAU is one part of the network information management system.

本课题采用基于客户／服务器和三层结构的ASP技术，在系统调研、需求分析的基础上，给出了基于互联网的省高校科研管理系统的设计目标、任务和原则；本系统的开发遵循完整性、通用性、实用性、先进性和开放性的原则，采用较为先进的结构化系统分析方法对数据流程、数据结构进行详尽的分析，制定一个适合高校科研管理的逻辑模型；提出一套基于互连网络的、采用大型数据库开发的科研管理系统的整体方案；系统设计采用Internet／Intranet全新技术，可做到全省高校科研信息集成与共享；研究制定我省高校科研管理信息系统的拓扑结构及应用平台，利用试点单位，设计和制定我省高校科研管理信息系统的功能结构和信息资源结构。

Objective To investigate the relationship between ovum fertilization rate and sperm morphology, acrosome reaction in IVF and to explore the influence of parameters of sperm on fertilization rate.

目的 研究体外受精(in vitro fertilization，IVF)中精子形态、顶体反应与卵子受精率之间的关系，分析精子各参数在IVF受精率方面的参考意义。

A: I hear Mr. White wants to run for the Presidency!

我听说怀特先生要参加总统竞选！