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The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12 000 independent transformant lines were acquired by the above method, from which two mutants related to plant defense response and development, asr1 (auxin and .salicylic acid responsive 1.) and dai1 (brought tolerant and obscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料，以激发标签为技术平台，构建了拟南芥的功能获得突变体库，从中筛选具有抗病、抗逆表型的突变体，克隆基因并进行基因的功能研究，取得的主要结果如下：(1)利用含激发标签质粒pSK1015的农杆菌直接转化拟南芥植株，以抗除草剂的Bar基因为筛选标记，获得了约有12000个独立转化株系的突变体库，并从中筛选到2个与抗病、抗逆和发育相关的突变体asr1(auxin and salicylic acid responsive 1)和dai1(drought tolerant and abscisic acid insensitive 1)。

The main work of this dissertation is constructing Arabidopsis gain of function mutants pool, screening mutants related to plant defense response, cloning the genes and studying their functions. The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12000 independent transformant lines were acquired by the above method from which two mutants related to plant defense response and development, asr1 (auxin and salicylic acid responsive 1) and dai1 (drought tolerant and abscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料，以激发标签为技术平台，构建了拟南芥的功能获得突变体库，从中筛选具有抗病、抗逆表型的突变体，克隆基因并进行基因的功能研究，取得的主要结果如下：(1)利用含激发标签质粒pSKI015的农杆菌直接转化拟南芥植株，以抗除草剂的Bar基因为筛选标记，获得了约有12000个独立转化株系的突变体库，并从中筛选到2个与抗病、抗逆和发育相关的突变体asrl(auxin and salicylic acid responsive l)和dail(dought tolerant and abscisic acidinsensitive l)。

The exacerbations and remissions will be indicated by the diseases, the seasons of the year, the reciprocation of the periods, whether they occur every day, every alternate day, or after a longer period, and by the supervening symptoms; as, for example, in pleuritic cases, expectoration, if it occur at the commencement, shortens the attack, but if it appear later, it prolongs the same; and in the same manner the urine, and alvine discharges, and sweats, according as they appear along with favorable or unfavorable symptoms, indicate diseases of a short or long duration.

根据疾病本身、季节变化、周期交替——无论是每天、每隔一天或更长时间——以及根据意外出现的症状，即可看出疾病是恶化还是解除。以胸膜炎病症为例，若咯淡出现于初期，则疾病不会长久；但若出现于后期，则疾病将会拖延。同样，根据大小便、出汗的现象及与之相伴的有利或不利症状，即可知患病时间之长短。

D. polymorpha Some previous studies have reported that mussel tolerance to pollutants is regulated by seasonal changes (Van Benschoten et al., 1995) and by temperature (Harrington et al., 1997; Buschini et al., 2003a), since temperature-dependent metabolic and filtration rates (Quigley et al., 1993; Kilgour and Baker, 1994) can modulate exposure to toxicants.

四多形 先前的一些研究报告说，淡菜耐受污染物是调节季节性变化（范benschoten等基地， 1995年）和温度（哈林顿等人， 1997年；布斯基尼等人， 2003年），因为温度依赖性代谢和过滤费率（奎格利等人， 1993年； kilgour和贝克， 1994年），可抑扬接触毒物。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

University of Jinan has 2,150 full-time faculty and staff with 150 professors, and 560 associate professors. 17 have been granted the government allowance by the State Council, 14 have been honored "Excellent Teacher" nationally, provincially and by the ministry.

学校在职教职工具有正高级职称的189人，副高级职称的632人；具有博士学位的227人，具有硕士学位的892人；有省、市级专业技术拔尖人才24人，省部级有突出贡献的中青年专家7名，享受政府特殊津贴人员18名，国家和省部级优秀教师 16名，荣获曾宪梓教育基金奖教师14名。

Therefore the signs acquire the names of things because they are mystical signs of sacred things, and because the signs and the things signified are sacramentally joined together; joined together, I say, or united by a mystical signification, and by the purpose or will of him who instituted the sacraments.

圣事联盟，因此有迹象收购的名字的东西，因为他们是神秘的迹象，神圣的东西，是因为交通标志和东西标志著都是sacramentally一起；一起，我说，还是团结在一个神秘的意义，以及由目的，或将他的人提起的圣礼。

The demons regrouped and retaliated, and dragons now began to fall from the sky, their bodies riddledby iron arrows and flaming bolt, by sorcerous poisons and by maddening visions. Still, the circle around

兵团恶魔企图组织反攻，巨龙开始从空中降落，他们的身体不怕火箭，抵抗毒药和低级魔法，巨龙越逼越近，向恶魔们宣泄着龙族的复仇之焰。

Based on the 1992-2007 remote sensing images and field survey data, and by using the landscape ecology theories and the method of conversion matrix, the spatiotemporal dynamics and landscape pattern of Spartina alterniflora in Yancheng coastal wetlands were analyzed by GIS. The results showed that the total area of S.

基于1992-2007年的遥感影像和野外调查数据，在地理信息系统的支持下，应用景观生态学理论和转移矩阵方法，定量分析了江苏盐城滨海湿地外来种互花米草的时空动态及其景观格局的演变规律。

Among them,Spermophilus dauricus was diurnal animal,and a long activity interval for avoiding strong sunlight at noon was observed.Microtus maximowiczii was active all day along,and its activity intensity by day and by night was similar,which may be a result of its adaptation to the habitat and feeding environment.

结果表明，达乌尔黄鼠为昼行性鼠种，中午时段出现明显的活动间歇期以避开强烈的日晒；莫氏田鼠为昼夜活动型鼠种，日夜活动强度差异不显著，与其生活于草滩底部营食草根的生活方式相适应。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。