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RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

结果用 PCR从酵母基因组DNA中扩增出大小约1.5 kb的suc2基因片段，序列测定表明除585位有一点突变外，所得suc2基因与文献报道相同，构建的定位突变载体导入酵母细胞后，得到4株营养型符合的突变体，PCR表明这些突变体中均有suc2基因的同源重组，取其中1株的PCR产物进行序列测定证实了同源重组的发生。

There's this image in the public consciousness that they're just a bunch of lugubrious , free-spending bodies mindlessly moving cheek to jowl and wasting the public's money, but that was not the Congress that I saw.

在公众的意识当中，拿些人是一群可悲的，挥霍无度的躯壳，他们说话不经过大脑，浪费公众钱财；但是我实际看到的议会不是这样的。

But here saying 'grasshopper' eat is really unheard of, but that's down something new.

但是这里说'蝗虫'吃的，真是闻所未闻的，但是这下新的东西。

It is true that our contract with our label is over but, that does not mean that the group automatically just breaks up...

对我们来说这只是一个开始，我们和公司的和约是停止了，但不代表这个组合会解散…哈哈哈

But I tell you, that will also lead to more depression and guilt if you are a child of God.

But我告诉你，这也将导致更多的抑郁症和内疚如果你是一个孩子的上帝。

Habakkuk knew that God's judgment upon Judah would not only mean that many would be killed, but that there would also be a captivity.

哈巴谷很清楚上帝对犹大的审判所意味的，不只是很多人要被杀，也指出很多人要被掳。

Mayor said many more planets meeting scientists' requirements for habitability would be found, but that that the most significant short-term discovery would be that of a low-mass planet even more similar to Earth.

梅杰说，将有很多符合科学家对可居住性要求的行星被发现，但最为重大的短期发现为该与地球极为类似的小型行星的发现。581c约为地球的5倍重，但仍是目前发现的最小的外行星。

"But that isn't what you want when you are trying to eliminate cancer cells," said Heaney, who notes that cancer patients should eat a healthy diet, which includes foods rich in vitamin C. It is use of large doses of over-the-counter vitamin C that is worrisome, he says.

"但是，这不是你想要当你正试图消除癌细胞，说："希尼，谁指出，癌症患者应该多吃健康的饮食，其中包括食物丰富的维生素C是使用大剂量超柜台维生素C这是令人担忧，他说。

Hiya: I personally don't like ForGG.. not the person himself, but playing against him. He says he won me everytime, but that was a long time ago.

金鱼：我不喜欢4哥，不是针对他的人，而是不喜欢跟他打，他说他每次都赢我，但这已经是很久以前的事了。

But team boss Christian Horner said: In terms of ultimate performance we don't feel it has earned its place on our car yet but that's not to say it will not do in the future.

但是车队老板霍内尔说：从最终表现我们并不认为它已经赢得了自己的地位对我们的赛车，但是这并不表示它不会在未来。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。