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She is not in the washroom and then stopping, because it is finally his own point of view that he cares about and not hers, and with this firmly in mind, and a light, confident step, such as you might find in the works of Bulwer-Lytton, he enters the space she occupies so prettily and, taking her by the hand, proceeds to tear off the stiff white hospital gown (but no, we cannot have that kind of pornographic merde in this majestic and high-minded sentence, which will probably end up in the Library of Congress)(that was just something that took place inside his consciousness, as he looked at her, and since we know that consciousness is always consciousness of something, she is not entirely without responsibility in the matter) so, then, taking her by the hand, he falls into the stupendous white puree of her abyss, no, I mean rather that he asks her how long it has been since her last visit, and she says a fortnight, and he shudders, and tells her that with a condition like hers (she is an immensely popular soldier, and her troops win all their battles by pretending to be forests, the enemy discovering, at the last moment, that those trees they have eaten their lunch under have eyes and swords)(which reminds me of the performance, in 1845, of Robert-Houdin, called The Fantastic Orange Tree, wherein Robert-Houdin borrowed a lady's handkerchief, rubbed it between his hands and passed it into the center of an egg, after which he passed the egg into the center of a lemon, after which he passed the lemon into the center of an orange, then pressed the orange between his hands, making it smaller and smaller, until only a powder remained, whereupon he asked for a small potted orange tree and sprinkled the powder thereupon, upon which the tree burst into blossom, the blossoms turning into oranges, the oranges turning into butterflies, and the butterflies turning into beautiful young ladies, who then married members of the audience), a condition so damaging to real-time social intercourse of any kind, the best thing she can do is give up, and lay down her arms, and he will lie down in them, and together they will permit themselves a bit of the old slap and tickle, she wearing only her Mr.

然后阻止，因为到最后那是他自己的关心的观点而不是她的，在他的脑子里根深蒂固，他自信地走了一步，这就像你也许会在布尔沃？里顿的作品中看到的一样，他进入兴高采烈地进入她占有的空间，抓住她的手，开始撕开他白色僵直的病号衣（但是我们没有在他冠冕堂皇的充满智慧的语句中发现那种带有色情的废话，那也许会在国会图书馆中绝迹）（在他的意识中有一些东西正在被替代，当他看她的时候，就如我们知道的意识始终是意识，她在这个事情中不是完全没有责任的），所以，然后抓起她的手，他掉进了她深渊的巨大白色水中，不，我想他问她自从她上回来已经多久了，她说有两个星期了，他战栗着告诉她她现在的情况（她是一个非常受欢迎的女兵，她的部队通过埋伏在森林里赢得了所有的战斗，在最后的时刻敌人发现了，那些他们吃掉他们午餐的树有了眼睛和剑）（这使我想起了一八四五年罗伯特？乌丹的演出，叫做《奇幻橘子树》，在其中罗伯特？

She is not in the washroom and then stopping, because it is finally his own point of view that he cares about and not hers, and with this firmly in mind, and a light, confident step, such as you might find in the works of Bulwer-Lytton, he enters the space she occupies so prettily and, taking her by the hand, proceeds to tear off the stiff white hospital gown (but no, we cannot h39e that kind of pornographic merde in this majestic and high-minded sentence, which will probably end up in the Library of Congress)(that was just something that took place inside his consciousness, as he looked at her, and since we know that consciousness is always consciousness of something, she is not entirely without responsibility in the matter) so, then, taking her by the hand, he falls into the stupendous white puree of her abyss, no, I mean rather that he asks her how long it has been since her last visit, and she says a fortnight, and he shudders, and tells her that with a condition like hers (she is an immensely popular soldier, and her troops win all their battles by pretending to be forests, the enemy discovering, at the last moment, that those trees they h39e eaten their lunch under h39e eyes and swords)(which reminds me of the performance, in 1845, of Robert-Houdin, called The Fantastic Orange Tree, wherein Robert-Houdin borrowed a lady's handkerchief, rubbed it between his hands and passed it into the center of an egg, after which he passed the egg into the center of a lemon, after which he passed the lemon into the center of an orange, then pressed the orange between his hands, making it 56aller and 56aller, until only a powder remained, whereupon he asked for a 56all potted orange tree and sprinkled the powder thereupon, upon which the tree burst into blossom, the blossoms turning into oranges, the oranges turning into butterflies, and the butterflies turning into beautiful young ladies, who then married members of the audience), a condition so damaging to real-time social intercourse of any kind, the best thing she can do is give up, and lay down her arms, and he will lie down in them, and together they will permit themselves a bit of the old slap and tickle, she wearing only her Mr.

然后阻止，因为到最后那是他自己的关心的观点而不是她的，在他的脑子里根深蒂固，他自信地走了一步，这就像你也许会在布尔沃？里顿的作品中看到的一样，他进入兴高采烈地进入她占有的空间，抓住她的手，开始撕开他白色僵直的病号衣（但是我们没有在他冠冕堂皇的充满智慧的语句中发现那种带有色情的废话，那也许会在国会图书馆中绝迹）（在他的意识中有一些东西正在被替代，当他看她的时候，就如我们知道的意识始终是意识，她在这个事情中不是完全没有责任的），所以，然后抓起她的手，他掉进了她深渊的巨大白色水中，不，我想他问她自从她上回来已经多久了，她说有两个星期了，他战栗着告诉她她现在的情况（她是一个非常受欢迎的女兵，她的部队通过埋伏在森林里赢得了所有的战斗，在最后的时刻敌人发现了，那些他们吃掉他们午餐的树有了眼睛和剑）（这使我想起了一八四五年罗伯特？乌丹的演出，叫做《奇幻橘子树》，在其中罗伯特？

On the other hand, beautiful thoughts of all kinds crystallize into habits of grace and kindliness, which solidify into genial and sunny circumstances; thoughts of courage, self-reliance, and decision crystallize into manly habits, which solidify into circumstances of success, plenty, and freedom; gentle and forgiving thoughts crystallize into habits of gentleness, which solidify into protective and preservative circumstances; loving and unselfish thoughts crystallize into habits of self-forgetfulness for others, which solidify into circumstances of sure and abiding prosperity and true riches.

截然相反的是，各种美好的思想会转变成坚强的习惯，它们会促成充满欢乐的环境；勇敢，自信与果断的思想会转变成坚强的习惯，它们会巩固充满成功，富足与自由的环境；文雅，宽大的思想会转变成温和的习惯，它们能进一步推进安全的，有保障的环境；充满爱心的，慷慨的思想转变成无私的习惯，它们会形成稳定，繁荣，真正富足的环境。——摘自《痛苦是错误思想的结果》詹姆斯。艾伦

A steel bar is washed and dried, and then put into a glass pipe which is sealed to obtain stirring magneton; a mixed solvent of Gamma-methacrylate oxide propyl trimethoxy silane and methanol is prepared and added with an organic base to obtain a pre-bonding solvent; the stirring magneton is immerged into the pre-bounding solvent, and the outer surface of the glass pipe of the stirring magneton is bonded with a layer of silane substance with alkenyl; a reactive monomer mixture and a pore-forming agent mixed solvent are evenly mixed and then added into a container, the stirring magneton after the pre-bounding is put into the container which is sealed and then put into a water bath; after reaction, the container is removed, the stirring magneton coated with a polymer is taken out and put into a extractor and extracted by using a solvent; or the stir bar is immersed into the solvent till no impurity can be checked in the solvent, thereby obtaining the product.

将铁条清洗干燥，放入玻璃管内，密封得搅拌磁子；配制γ-甲基丙烯酸氧丙基三甲氧基硅烷与甲醇的混合溶液，加入有机碱得预键合溶液，将搅拌磁子浸入预键合溶液，在搅拌磁子的玻璃管外壁键合一层带烯基的硅烷化物质；将反应单体混合剂和致孔剂混合溶液混匀后倒入容器中，将预键合后的搅拌磁子放入容器，密封后置于水浴中，反应后除去容器，取出涂有聚合物的搅拌磁子，置于提取器中，以溶剂提取，或将搅拌棒置于溶剂中浸泡至溶剂中无杂质检出，得产物。

Dis- Fly away lonely souls into the endless sky so far away When you're all alone Feeling down and lonely Tell me what you see deep inside your soul You only know who you are So don't be afraid Just open your heart to me You will spread your wings like an eagle Learning how to fly in the sky Just like the sun rises after the rain you'll shine in the air Take off your mask and be true to your heart trust yourself and fly away Into the light of joy and truth Where there's a grief there will always be a hope For a place where we can be who we wanna be Your tears may fade away But always keep the faith and live tomorrow Break through the night away into a brand new day Life's just begun Yeah, feelin' the melody of eternity It has the power to set us free Travlin' through the galaxy with only space around Follow the suns peaceful sound It's time for us to end this illusion Fightin' ourselves will only cause confusion Shinin' light is the key of life Let's all get together and make this better I don't know how but there has got to be a way There are times when I Wish I could just disapear Far from this place of pain and hurt If only I could be gone There's something in your eyes Pulls me back from running away Oh, yes it's hard sometimes But now I'm strong enough to tell the sadness goodbye Just like the moon fills the night with the stars I'll dance in the air Seek in the darkness there's nothing to fear I will stand and fly away Into the night I'll find the truth The pain never ends but there's always be a hope To be free eternally in this galaxy To live another day the sun will show the way and I will follow Break through the night away into a brand new day My journey goes on Into the light of joy and truth Where there's a grief there will always be a hope For a place where we can be who we want to be Your tears may fade away But always keep the faith and live tomorrow Break through the night away into a brand new day Your journey's begun

阴间的神-飞孤单的灵魂进入在如此远处的永无止境的天空之远之内当你全部很孤独的时候感觉下来和孤单的告诉我你在你的灵魂里见到起来深的你只知道你是谁如此不害怕仅仅对我开放你的心你将会像一只鹰一样传布你的翅膀学问该如何在天空中飞仅仅在雨之后喜欢太阳上升你将会在空气中发亮脱掉你的假面具而且忠于你的心信赖你自己和飞行之远进入欢喜和事实的光之内哪里总是有伤心事在那里意志是一个希望对于我们能是的一个地方我们想要当谁你的泪滴可能褪色离开但是总是保存信心和活的明天进入商标新的日子之内夜晚之久突破仅仅被开始的生活是的，feelin'永恒的曲子资讯科技有力量释放我们 Travlin'经过银河由于只在附近隔开跟随太阳和平的声音资讯科技是我们结束这一个幻影的时候 Fightin'我们自己只将会引起混乱 Shinin'光是生活的钥匙全部让我们聚在一起而且使这变成更好我不知道如何除了那里之外已经开始是一个方法有时代此时我希望我可以正直的 disapear 离痛苦和伤害的这一个地方很远如果只有我可能不见了某事在你的眼睛中从逃走把我拉回来哦，是的资讯科技努力地有时是但是现在我够强壮来告诉悲哀再见仅仅喜欢，月亮将夜晚装满星我将在空气中跳舞黑暗的找寻没有东西害怕，我将会站而且飞离开进入夜晚之内我将找事实痛苦从不结束但是总是有是一个希望在这银河中是自由地永恒地在另外的一个日子居住太阳将会表示方式而且我将会跟随进入商标新的日子之内夜晚之久突破我的旅程继续进入欢喜和事实的光之内哪里总是有伤心事在那里意志是一个希望对于我们能是的一个地方我们想要当谁你的泪滴可能褪色离开但是总是保存信心和活的明天进入商标新的日子之内夜晚之久突破你的被开始的旅程

The carbons in explosives are decomposed into gas-like carbon atoms in the detonation reaction zone, then unoxidized carbon atoms coagulate into carbon liquid droplets and finally consolidate into solid diamonds The new mechanism differs from one conclusion that carbons of explosives are at first transformed into solid graphites, then into diamonds, and also differs from the conclusion that gaseous carbons coagulate into liquid droplets in the reaction zone, then crystalize into diamonds in Taylor rarefaction wave zone.

修正了有关炸药中的碳在反应区内先转变为石墨，然后石墨再发生相变生成金刚石的论点以及认为在反应区内气态碳凝聚为碳液滴，液滴在Taylor稀疏波区凝固成金刚石的论点。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Hey yo jim man why don't ya, you don't you kick some of that you know you know how you do it man it's a trip people don't even believe were together right now but tell your story you know the one i like say it for me riders on the storm riders on the storm into this house we're born (into this house we're born) into this world we're thrown (into this world we're thrown) like a dog without a bone an actor out on loan riders on the storm there's a killer on the road his brain is squirmin' like a toad take a long holiday let your children play if ya give this man a ride sweet family will die killer on the road, yeah goin' off of this off of that with the lizard king bumpin' in the back how bout that driftin', liftin', swiftin', coastin', testaroastin' but the wheels won't stop 200 on the highway fresh up off the block he's a rider, na he's a killer dresses in all black but his hat says stealla pedal to the metal i gotta go hard drive by and say hello hey fredwreck you my mello now let me here what i sound like acapella wow ride dip swish now bring it back just like this like a dog with out his bone unlike a g with out his chrome it's hard to imagine the homey dog in a jag and he's checkin' for the checkered flag comin' in first never in last cause my car to fast i neva eva run out of gas cause i just to clean i do it upper class so fasten your seat belts its so hot it will even make heat melt so get a bowl and roll and ride slip through the slip and slide like a dog without a bone an actor out on loan riders on the storm there's a killer on the road his brain is squirmin' like a toad take a long holiday let your children play if ya give this man a ride sweet family will die killer on the road, yeah need for speed i'm trying to take the lead hold on little homey before you run into the trees i've seen thinks that i would have never saw before hey yo jim let'em in, let'em in open up my back tire smokin' the whole street and now the police wanna flash there lights and chase the dogg all night but i won't pull over nor give up cause i just don't give a fuck yeah from the side boy where we was born and raised straight up to ride boy continuously, we get to an expeditiously keep the light on east side on snoop dogg and the doors and yeah we bout to ride on riders on the storm riders on the storm into this house we're born into this world we're thrown were thrown

嘿摇摇弯轨机人为什么不唉，你不你踢一些那你知道你知道如何你这样做人这是一次旅行人们甚至不相信是在一起此时但告诉你的故事你知道那个我像为我说它骑士在暴风雨上骑士在暴风雨上入这房子我们是出生入这世界我们是扔像个狗没有一根骨头一个演员那儿有一个杀手在路上他的大脑是squirmin'像个癞蛤蟆采取一个长的假期让你的孩子们玩如果唉给这人一个乘甜家将死凶手在路上，是呀去'离开对于这离开关于那和蜥蜴国王 bumpin'在后面如何拳击比赛那 driftin'，liftin'，swiftin'，coastin'，testaroastin'但是这个车轮不会停止两百在公路上新鲜向上远离块他的一个骑士，娜他的一个杀手衣服在纯黑但他的帽子说stealla 踩踏板金属我有去难驶过和问好嘿fredwreck 你我的梅洛现在让我这里什么我听起来像acapella 哇骑现在倾斜豪华带来它回来只是这样像个狗与出来像个狗没有一根骨头一演员借出的骑士在暴风雨上那儿有一个杀手在路上他的大脑是squirmin'像个癞蛤蟆采取一个长的假期让你的孩子们玩如果唉给这人一个乘甜家将死凶手在路上，是呀需要速度我是试图着先鞭继续小像家一样在你未碰撞树木我已经看到想那我从来不锯在以前嘿摇摇弯轨机让'em 在，让'em 在开发我的后面疲劳smokin'一街一巷那么警察要闪光那儿灯光和追逐道格整夜但我不会拦截也不放弃造成我只是不给一个性交是呀自边男孩我们在那里出生和提升直直至骑男孩骑士在暴风雨上骑士在暴风雨上入这房子我们是出生入这世界我们是扔我的翻译不太标准别见怪

You will miss th-e time you spent together when you are outward alone If I could catch a rainbow If I could catch a rainbow Icould do just for you And share with it's beauty Onthe days you are felling blue IF I could build a mountain you ould call you very own A place to find serenity A place to be alone If I could take your troubles Icould toss them into the sea out all these things I'm finding Are possible for you I can't build a mountain or catch a rainbow fair Butlet me know what I know best A friend that's always there

You会错过第娥一起度过的时间可以当你向外单独如果我能赶上彩虹如果我能赶上彩虹Icould做只是为了你和分享它的美丽Onthe天你是砍树如果我能建立一个山区你乌勒德呼吁蓝你非常的一个地方找到宁静的地方独处如果我可以采取你的麻烦Icould扔到海中的所有这些事情，我发现它们有可能是你我不能建立一个山区或捕捉彩虹公平Butlet我知道，我知道最好的朋友，总是有

If I could catch a rainbow 如果我能留住彩虹 If I could catch a rainbow I would do it just for you And share with its beauty On the days you're feeling blue. If I could build a mountain You could call your very own A place to find serenity A place to be alone. If I could take your troubles I would toss them into the sea But all these things I'm finding Are impossible for me. I cannot build a mountain Or catch a rainbow fair But let me be what I know best A friend that's always there.

如果我能留住彩虹如果我能留住彩虹我将只为你一个人挽留在你感到忧伤的日子与你分享它的美丽如果我能建造大山你尽可把它当成你自己的体验宁静的空间独处的地方如果我能带走你的烦恼我会把它们通通扔进大海然而我发现所有这些事情我都无能为力我建不成一座大山也留不住彩虹的美丽就让我做你最好的朋友吧永远与你相伴生词： could--can 的过去式-conj。

Although translator has turned from being a crystal ball by which the original culture can unrestrainedly penetrate to another crystal ball by which the target culture can freely traverse, the translator's personal embodiment, in the process of cognitive act, are still absent in translation studies. Translators are still subjects without body or simply disembodied subjects.

译者虽然由原语文化可以自由穿透的玻璃球变成了译语文化可以自由穿越的玻璃球，但译者认知过程中的个体体验在翻译研究中依然缺席，译者依然仅仅是一个没有躯体体验的主体。

Chillingly, he claimed our technology is 'not nearly as sophisticated' as theirs and "had they been hostile", he warned 'we would be been gone by now'.

令人毛骨悚然的，他声称我们的技术是'并不那么复杂，像他们一样，和"如果他们敌意"，他警告说，'我们将现在已经过去了。