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In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2~0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%~80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0~0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法,将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合,建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺:胰酶用pH4.5醋酸缓冲溶液提取后,粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液:0.01mol/LNaoAc-HoAc缓冲液(pH4.5,洗脱缓冲液:0.01mol/LNaoAc-HoAc缓冲液(pH4.5)含0.2~0.35mol/LNaCl分两步洗脱,收集激肽释放酶的洗脱峰;DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液:0.01mol/LTris-HCl缓冲液(pH7.5,洗脱液:0.5mol/LNaCl溶液,一次洗脱,收集胰蛋白酶洗脱峰;最后,亲和层析未吸附液用30%~80%硫酸铵分级盐析处理,沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液:0.01mol/LNaAc-HAc缓冲液(pH4.5含1mol/L(NH_4)_2SO_4,洗脱缓冲液:0.01mol/LNaAc-HAc缓冲液(pH4.5)含0~0.6mol/L(NH_4)_2SO_4,分两步洗脱,收集糜蛋白酶的洗脱峰。

The main reason why American mutual fund is so advanced is that there is the concerted mechanism environment for mutual fund, such as the mature financial market, sufficient investment capital supply, well-developed system of laws and so on. Adopting the specific exponents of Shanghai and Shenzhen Security Market, fund exponent yields, all weekly yields of mutual fund in specific periods, etc., the positive analysis of the coordination of Chinese mutual fund and Security Market demonstrates that this coordination is not ideal generally. On condition that the market characteristic of mutual fund is made clear, mutual fund can develop conceitedly and healthily by developing security market, increasing market efficiency, providing more fund, improving fund management, standardizing relative system of privately offered funds and so on, and optimizing the market environment to develop mutual fund.

美国开放式基金之所以发达,主要原因在于开放式基金具有协调发展的制度环境,如成熟的金融市场、充足的投资资金供给、完善的法律体系等;采用沪、深证券市场特定指数与基金指数收益率,及特定时期内所有开放式基金的周收益率等指标,对我国开放式基金与证券市场的协调性的实证分析发现,二者协调性程度总体上不理想;在明确开放式基金发展的市场过程性质的前提下,通过促进证券市场的成熟、提高市场效率、完善法律、拓宽基金资金供应渠道、完善基金治理结构、规范私募基金发展等配套措施,优化开放式基金发展的市场环境,才能不断促进开放式基金的协调、健康发展。

This invention discloses a multi-queue sequence buffer management circuit and a method based on a pipeline applying a pipeline structure including: an arbitration circuit selecting one for process from read, write and distribution buffer requests, a buffer slot state module designing state of the slot requiring operation and queue numbers and assigning idle slots, a buffer slot filter module filtering the slot, a buffer slot filter module filtering the slot states not belonging to the current operation queues nor idle aligned in terms of the head pointer, a queue slot selection module computing continuous idle slot numbers from the slot pointed by the head pointer and refreshing the head pointer and selecting preparing slots, a queue slot prior queuing module refreshing the read pointer and result numbers of the current operation queues with the pointer of the first prepare slots and their numbers which can support multi-queue to share one buffer space, queues can access the buffer in overlap.

本发明公开一种基于流水线的多队列顺序化缓冲管理电路及方法,本发明电路采用流水线结构,包括:仲裁电路,从读、写、分配缓冲请求中选取一路进行处理;缓冲槽口状态设置模块,设置请求操作的槽口状态和队列号,分配空闲槽口;缓冲槽口滤除模块,滤除不属于当前操作队列且非空闲态的槽口状态,按头指针对齐;队列槽口选择模块,计算头指针指向槽口起的连续空闲槽口数并更新头指针,选出预备态的槽口;队列槽口优先排队模块,用第一个预备态槽口的指针和预备态槽口数分别更新当前操作队列的读指针和结果数;本发明可以支持多个队列共享一个缓冲空间,各类指令队列能对缓冲进行交叉访问,并对指令结果的写入读出进行顺序化管理。

Through a combination of T7 RNA polymerase and T7 Promoter for testing proper buffer was selected.The experimental results showed 5×binding-Buffer 1 was more suitable for the test;TBE running-buffer was better than TAE and TGE buffer;0.2×TBE buffer was the best concentration amang there electrophoresis buffer.

通过用T7RNA聚合酶与T7启动子DNA的结合反应来选择合适的缓冲液,结果表明,不同的缓冲液会影响凝胶迁移试验的结果,5×Buffer1结合缓冲液的效果较好;TBE电泳缓冲液比TAE、TGE的电泳效果好,同时在3种不同浓度的TBE缓冲液中,0.2×的TBE效果最好。

Trans-Blot Cells and Systems 170-3825 Trans-Blot Cell With Wire Electrodes and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3850 Trans-Blot System With Plate Electrodes and PowerPac HC Power Supply, 100120/220240 V 170-3853 Trans-Blot System With Plate Electrodes, Super Cooling Coil, and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3910 Trans-Blot Cell With Wire Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3939 Trans-Blot Cell With Plate Electrodes and Super Cooling Coil, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3946 Trans-Blot Cell With Plate Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) Trans-Blot Cell Accessories 170-3912 Super Cooling Coil, required for all high-intensity transfers 170-3913 Gel Holder Cassette, includes 2 fiber pads 170-3914 Fiber Pads, 15.5 x 20.5 cm, 6 170-3920 Trans-Blot Standard Wire Electrode Card, cathode 170-3921 Trans-Blot Standard Wire Electrode Card, anode 170-3922 Trans-Blot Cell Buffer Tank 170-3923 Trans-Blot Cell Lid With Power Cables 170-3943 Trans-Blot Platinum Anode Plate Electrode 170-3944 Trans-Blot Stainless-Steel Cathode Plate Electrode 170-3945 Trans-Blot Plate Electrode Pair, platinum anode and stainless-steel cathode 16 规格:说明: Trans-Blot Plus

电泳转印槽组件 1。缓冲液槽及带有电缆的盖 2。凝胶支架转印夹 3。纤维衬垫 4。电极丝 5。电极板 6。特级冷却芯 Trans-Blot 转印槽是功能灵活的转印设备,可理想地用于多种转印应用。Trans-Blot 转印槽特点包括:*能进行多胶转印,可容纳3 块PROTEAN I xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶*多组参数灵活可设,可调节的电压设置(从30 V 的过夜转印到200 V 的1 小时快速实验)*电极间距设置为8 cm 用于标准印迹杂交,或设置为4cm 用于高强度印迹杂交*可选择板式电极:涂有铂金的钛作为正极,不锈钢为负极,能提供高强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝*通过特级冷却芯和水循环仪来调节温度―是天然酶(4°C)或高强度转印的理想选择,随着转印时间增加(多达24 小时),不会引起缓冲液耗竭(在高强度转印中必须使用冷却芯,也推荐用于所有板式电极的应用)*带铰链的凝胶支架转印夹能避免滑动,确保凝胶与印迹膜间的紧密接触;每个转印夹都有颜色标记以保证在转印槽中的正确定位 Trans-Blot 转印槽的锁闭凝胶支架转印夹系统。转印夹(1)支撑凝胶(2)印迹膜(3)两侧有纤维衬垫和滤纸(4)确保凝胶三明治内的完全接触。凝胶夹垂直插入缓冲液槽中(5)。

For fund companies and their employees in investment funds, Fund for the Department of the SFC in June last year issued a "Circular on fund investment in securities investment funds of employees of matters relating to notice", which provides that "the fund management companies should be in the fund contract commencement notice traded funds notice book and related semi-annual report and annual report disclosure of fund employees of the Company held by the Fund's total share and accounted for the only the proportion of the overall share of the Fund."

对基金公司和投资基金,为证监会基金部于去年6月发表了他们的雇员在证券投资基金的通知"的有关通知"的问题员工,投资基金的规定"基金管理公司应当在基金合同生效公告书买卖基金的通知和相关的半年度报告和基金的总份额的基金持有本公司员工年度报告披露和唯一的总份额中所占比重该基金的。"

This paper analyses the homogenous tendency of open-end fund at first,pointing out that unperfect development of market and investors' immature investment opinions is the origin of such phenomena. Secondly,having compared the fund sizes and purchase fee rates of 25 open-end funds in China,conclude that there are problems of diseconomy of scale and indistinctive relativity between fees and achievement in Chinese open-end funds. Then this paper introduces benefit and cost function to analyze the problem of lack of motivation and constrain mechanism towards fund managers because of unreasonable fees in China. Thirdly,this paper analyze funds managers' normal hazards by static game model,and then points out fund managers have serious tunneling behaviors in Chinese open-end fund industry. In the end,this paper analyzes the causes of liquidity risk of open-end fun and the particularity of liquidity risk of open-end fund in China,and concludes that liquidity risk in Chinese open-end fund industry is higher through calculating the rates of share change and comparing the portfolio selections of top 20 open-end funds in China.

本文首先分析了我国目前存在的基金产品同质化现象,指出市场发展的不完善以及投资者投资理念的不成熟是同质化的根源所在;其次对比了我国现有的25只开放式基金的规模和申购费率,得出了我国开放式基金存在着规模效应不明显以及基金业绩与费率相关性不强的问题,并引入效益函数和成本函数分析了由于我国开放式基金费率不合理引发的基金管理者激励约束机制缺失的问题;再次,应用静态博弈模型分析了基金管理人面临的道德风险,指出目前我国开放式基金由于监管机制脆弱而存在着较为严重的管理者利益输送行为;最后,分析了开放式基金流动性风险的形成原因以及我国基金市场流动性风险的特殊性,并通过计算目前我国基金资产规模前20位的开放式基金的份额变动率以及比较它们的投资组合结构,得出了目前我国开放式基金流动性风险偏大的结论。

Reporters, according to preliminary statistics, is being issued in the new fund has 11, including the stock-Days Hong Yong set the value of growth funds, fund Xiang Yi Fang Dakota; configuration, including Golden Eagle-dividend value of the fund in the sea Blue-chip flexible allocation of funds, fund selection strategy Fu; bond type, including 10,000 in Paris Tim Shen Po-yi bonds A + B, Yinhua Fund to enhance revenue bonds, bond fund returns Oriental sound, stable and increased profits by HSBC Jin Xin Bond Fund, the Bank of farmers Agricole, and always will benefit by bond funds, the silver-AXA to optimize revenue bond funds.

记者,据初步统计,正在发行的新基金有11个,其中包括股票天闳壅设置的价值成长型基金,基金Xiang易伐嗯达科他州,配置,包括金鹰股息基金的价值在海蓝筹灵活分配资金,基金选择策略富;债券类型,包括万巴黎添申坡义债券甲+乙,银华基金,以提高收入债券,债券型基金回报率东方的声音,由汇丰靳稀嗯债券基金,农民银行法国农业,稳定和增加利润都将受益于债券基金,银安盛优化收益债券基金。

Article 88 Where a fund manager or a fund custodian, in violation of the provisions of this Law, fails to place the assets of a fund under separate management or custody in separate accounts or misappropriates the fund assets, it shall be instructed to rectify and shall be fined not less than 50,000 yuan but not more than 500,000 yuan Where losses are caused to the fund assets or to the holders of fund units, it shall, in accordance with law, bear the responsibility to pay compensation The persons who are directly in charge and other persons who are directly responsible shall be given a discipline warning, or be suspended or disqualified from engaging in the fund business and, in addition, be fined not less than 30,000 yuan but not more than 300,000 yuan If the violation constitutes a crime, it shall be investigated for criminal responsibility in accordance with law

第八十八条基金管理人、基金托管人违反本法规定,未对基金财产实行分别管理或者分账保管,或者将基金财产挪作他用的,责令改正,处五万元以上五十万元以下罚款;给基金财产或者基金份额持有人造成损害的,依法承担赔偿责任;对直接负责的主管人员和其他直接责任人员给予警告,暂停或者取消基金从业资格,并处三万元以上三十万元以下罚款;构成犯罪的,依法追究刑事责任

The invention discloses a LCD panel, colorful filter piece and making method, which comprises the following steps: providing a base; forming a buffer material layer on the base; forming patterned lightproof layer on the buffer material layer; adopting patterned lightproof layer as lid to pattern the buffer material layer to form a patterned buffer layer; forming a separating piece through patterned lightproof layer and patterned buffer layer; making the separating piece form multiple pixel areas on the base; forming a colorful filter layer in each pixel area.

本发明公开了一种液晶显示面板、彩色滤光片及其制作方法,其中彩色滤光片的制作方法包括下列步骤:首先,提供一基板,并在基板上形成一缓冲材料层。接着在缓冲材料层上形成一图案化遮光层。以图案化遮光层为罩幕来图案化缓冲材料层而形成一图案化缓冲层,使得该图案化遮光层与图案化缓冲层共同形成一隔离件,且隔离件在基板上形成多个像素区域。然后在每一像素区域内分别形成一彩色滤光层。

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推荐网络例句

Although translator has turned from being a crystal ball by which the original culture can unrestrainedly penetrate to another crystal ball by which the target culture can freely traverse, the translator's personal embodiment, in the process of cognitive act, are still absent in translation studies. Translators are still subjects without body or simply disembodied subjects.

译者虽然由原语文化可以自由穿透的玻璃球变成了译语文化可以自由穿越的玻璃球,但译者认知过程中的个体体验在翻译研究中依然缺席,译者依然仅仅是一个没有躯体体验的主体。

Chillingly, he claimed our technology is 'not nearly as sophisticated' as theirs and "had they been hostile", he warned 'we would be been gone by now'.

令人毛骨悚然的,他声称我们的技术是'并不那么复杂,像他们一样,和"如果他们敌意",他警告说,'我们将现在已经过去了。

And in giving such people " a chance to be themselves," he saw himself as a champion of th South's hardscrabble underclass, both black and white.

他给了这些人一个"成就自己"的机会,同时将自己看成是南方那些贫困的下层人民的声援者。