查询词典 buffer circuit

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

This invention discloses a multi-queue sequence buffer management circuit and a method based on a pipeline applying a pipeline structure including: an arbitration circuit selecting one for process from read, write and distribution buffer requests, a buffer slot state module designing state of the slot requiring operation and queue numbers and assigning idle slots, a buffer slot filter module filtering the slot, a buffer slot filter module filtering the slot states not belonging to the current operation queues nor idle aligned in terms of the head pointer, a queue slot selection module computing continuous idle slot numbers from the slot pointed by the head pointer and refreshing the head pointer and selecting preparing slots, a queue slot prior queuing module refreshing the read pointer and result numbers of the current operation queues with the pointer of the first prepare slots and their numbers which can support multi-queue to share one buffer space, queues can access the buffer in overlap.

本发明公开一种基于流水线的多队列顺序化缓冲管理电路及方法，本发明电路采用流水线结构，包括：仲裁电路，从读、写、分配缓冲请求中选取一路进行处理；缓冲槽口状态设置模块，设置请求操作的槽口状态和队列号，分配空闲槽口；缓冲槽口滤除模块，滤除不属于当前操作队列且非空闲态的槽口状态，按头指针对齐；队列槽口选择模块，计算头指针指向槽口起的连续空闲槽口数并更新头指针，选出预备态的槽口；队列槽口优先排队模块，用第一个预备态槽口的指针和预备态槽口数分别更新当前操作队列的读指针和结果数；本发明可以支持多个队列共享一个缓冲空间，各类指令队列能对缓冲进行交叉访问，并对指令结果的写入读出进行顺序化管理。

Through a combination of T7 RNA polymerase and T7 Promoter for testing proper buffer was selected.The experimental results showed 5×binding-Buffer 1 was more suitable for the test;TBE running-buffer was better than TAE and TGE buffer;0.2×TBE buffer was the best concentration amang there electrophoresis buffer.

通过用T7RNA聚合酶与T7启动子DNA的结合反应来选择合适的缓冲液，结果表明，不同的缓冲液会影响凝胶迁移试验的结果，5×Buffer1结合缓冲液的效果较好；TBE电泳缓冲液比TAE、TGE的电泳效果好，同时在3种不同浓度的TBE缓冲液中，0.2×的TBE效果最好。

Trans-Blot Cells and Systems 170-3825 Trans-Blot Cell With Wire Electrodes and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3850 Trans-Blot System With Plate Electrodes and PowerPac HC Power Supply, 100120/220240 V 170-3853 Trans-Blot System With Plate Electrodes, Super Cooling Coil, and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3910 Trans-Blot Cell With Wire Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3939 Trans-Blot Cell With Plate Electrodes and Super Cooling Coil, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3946 Trans-Blot Cell With Plate Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) Trans-Blot Cell Accessories 170-3912 Super Cooling Coil, required for all high-intensity transfers 170-3913 Gel Holder Cassette, includes 2 fiber pads 170-3914 Fiber Pads, 15.5 x 20.5 cm, 6 170-3920 Trans-Blot Standard Wire Electrode Card, cathode 170-3921 Trans-Blot Standard Wire Electrode Card, anode 170-3922 Trans-Blot Cell Buffer Tank 170-3923 Trans-Blot Cell Lid With Power Cables 170-3943 Trans-Blot Platinum Anode Plate Electrode 170-3944 Trans-Blot Stainless-Steel Cathode Plate Electrode 170-3945 Trans-Blot Plate Electrode Pair, platinum anode and stainless-steel cathode 16 规格:说明: Trans-Blot Plus

电泳转印槽组件 1。缓冲液槽及带有电缆的盖 2。凝胶支架转印夹 3。纤维衬垫 4。电极丝 5。电极板 6。特级冷却芯 Trans-Blot 转印槽是功能灵活的转印设备，可理想地用于多种转印应用。Trans-Blot 转印槽特点包括：*能进行多胶转印，可容纳3 块PROTEAN I xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶*多组参数灵活可设，可调节的电压设置（从30 V 的过夜转印到200 V 的1 小时快速实验）*电极间距设置为8 cm 用于标准印迹杂交，或设置为4cm 用于高强度印迹杂交*可选择板式电极：涂有铂金的钛作为正极，不锈钢为负极，能提供高强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝*通过特级冷却芯和水循环仪来调节温度―是天然酶（4°C）或高强度转印的理想选择，随着转印时间增加（多达24 小时），不会引起缓冲液耗竭（在高强度转印中必须使用冷却芯，也推荐用于所有板式电极的应用）*带铰链的凝胶支架转印夹能避免滑动，确保凝胶与印迹膜间的紧密接触；每个转印夹都有颜色标记以保证在转印槽中的正确定位 Trans-Blot 转印槽的锁闭凝胶支架转印夹系统。转印夹（1）支撑凝胶（2）印迹膜（3）两侧有纤维衬垫和滤纸（4）确保凝胶三明治内的完全接触。凝胶夹垂直插入缓冲液槽中（5）。

The high speed multiplex first-in first-out storage structure includes at least two memory unit arrays, one integrated decoder circuit between the two memory unit arrays, one write-in control circuit over the decoder circuit, one read-out control circuit below the decoder circuit, two data buffers on the two memory unit arrays separately, two multiplex circuits and two output circuits below the two memory unit arrays separately, and one write-1 clock buffer and one read-out clock buffer over and below the decoder circuit separtely.

一种高速多路先进先出存储器结构，包括一至少两存储单元阵列、一位于至少两存储单元阵列中间的整体解码电路、分别位于整体解码电路的上下的一写入控制电路及一读出控制电路、分别位于至少两存储单元阵列上的两数据输入缓冲器以及依序位于两存储单元阵列下的两多工电路及两输出电路，在整体解码电路的上下分别设置一写入时钟缓冲器及一读出时钟缓冲器。

The controller includes multi-channel switching circuit, interface and conversation circuit, input and output circuit, manual button control circuit, LCD driving circuit, real-time clock circuit, preparedness store circuit, keyboard processing circuit and dial switch circuit.

该控制器硬件包括多通道切换电路、接口及转换电路、输入输出电路、手动按钮控制电路、手动与自动切换电路、液晶显示驱动电路、实时时钟电路、预案存储电路、键盘处理电路、拨码开关电路。

Total circuit scheme and time sequence of tool design and implementation have been designed on the AIL logging characteristic. The design and analysis for circuit unit have been carry out on the several units such as main control circuit, signal phantom circuit, signal control circuit, amplifier circuit, A/D circuit, transmitter drive circuit and power circuit.

按照AIL的结构特点，进行了仪器总体设计，设计了仪器工作电路原理和工作时序，在此基础上，进行了主控制电路、信号幻像转换电路、信号控制电路、放大电路、A/D转换电路、发射驱动电路、电源电路等单元电路的分析和设计，从而完成了仪器单元电路的设计与实现。

This invention relates to a DC linear motor digital controller including a central processor circuit, a data memory, a program memory, an A/D conversion circuit, a D/A conversion circuit, a linear shift orthogonal code pulse signal circuit, a signal isolation circuit, among which, the center processor circuit is composed of a special DSP chip controlled by the motor, a complicated programmable logic device CPLD combining with a peripheral expanding circuit, said digital controller fully utilizes the motor to control the special DSP chip to integral DSP kernel CZXLP and the rich external circuit in a single chip to make up of a digital controller for controlling high frequency response DC linear motor with small volume, low dissipation and high reliability.

高频响直流直线电机数字控制器，属于电机自动控制技术领域，目的在于获得快速、高精度往返运动的控制效果，实现高频响直流直线电机的精密位置跟踪控制功能。本发明包括中央处理器电路，数据存储器，程序存储器，A/D转换电路，D/A转换电路，直线位移正交编码脉冲信号电路，信号隔离电路；中央处理器电路由一个电机控制专用DSP芯片，复杂可编程逻辑器件CPLD，结合外围扩展电路组成。该数字控制器充分利用电机控制专用DSP芯片将高性能DSP核C2xLP和丰富的功能外设电路集成在单个芯片上这一特点，构成了一个体积小、功耗低、可靠性高和功能比较齐全的高频响直流直线电机控制用的数字控制器，使系统的整体成本大大降低。

Set off from the function request analysing the instruction fuse, have done more detailed introduction first to it's every ingredient; The design and concrete parameter having followed , carrying out a scheme on the circuit directing fuse power source boosting circuit , host to get angry , electricity basics circuit such as insurance circuit , self-destroying circuit , self-deactivating circuit here on the basis, are chosen; Constitute every circuit optimization finally, having become a overall circuit module

从分析指令引信的性能要求出发，首先对其各组成部分作了较详细的介绍；随后，在此基础上，对指令引信的电源升压电路、主发火电路、电保险电路、自毁电路、自失能电路等基本电路进行了方案设计和具体参数选取；最后，将各电路优化组合成了一个整体电路模块。

Finally, took AT89S52 as the core, Designed contains the integration voltage-stabilizer circuit, the clock electric circuit, repositions the electric circuit, the liquid crystal display and the control circuit, ISP in-system programmable devices, the RS232 serial communication electric circuit in the system, the switching circuit, LED display circuit chip and the ISD2590 pronunciation chip recording/broadcast electric circuit and so on nine kind of periphery electric circuits, and compiles for the integrity schematic diagram.

最后，以AT89S52为核心，设计出包含集成稳压电路、时钟电路、复位电路、液晶显示与控制电路、ISP在系统可编程器、RS232串行通信电路、开关电路、发光二极管显示电路和ISD2560语音芯片的录音/播放电路等九种外围电路，并汇总为完整的原理图。

Although translator has turned from being a crystal ball by which the original culture can unrestrainedly penetrate to another crystal ball by which the target culture can freely traverse, the translator's personal embodiment, in the process of cognitive act, are still absent in translation studies. Translators are still subjects without body or simply disembodied subjects.

译者虽然由原语文化可以自由穿透的玻璃球变成了译语文化可以自由穿越的玻璃球，但译者认知过程中的个体体验在翻译研究中依然缺席，译者依然仅仅是一个没有躯体体验的主体。

Chillingly, he claimed our technology is 'not nearly as sophisticated' as theirs and "had they been hostile", he warned 'we would be been gone by now'.

令人毛骨悚然的，他声称我们的技术是'并不那么复杂，像他们一样，和"如果他们敌意"，他警告说，'我们将现在已经过去了。