查询词典 brood cell

After 6 to 7 days of culture, Eleven ES cell lines were selected and explanted. At last we obtained 4,3,4 and 3 ES cell lines separately. One of the B6C3F_1×B6D2F_1 ES cell lines was selected to future study its competent for germline transmission.We founded the ES cell line has normal karyotype on the chromosome analyse.In teratoma experiment by injected ESCs into C57 mices inguen, we gained teratoma contained cells come from all three embryonic germ layers.

选择一株具有高度杂合背景的B6C3F_1×B6D2F_1 ES细胞系进行核型分析、畸胎瘤实验后与ICR品系2n胚胎构建嵌合体并得到具有生殖系传递能力的嵌合体小鼠，证明此细胞系具有生殖系嵌合能力。

ResultsThe glandular cell in the endometrial implant after therapy with middle or high-dose of (10,15 gkg-1d-1) XiaoChaiHu Decoction showed characteristic features of apoptosis which displayed by the cell decreased in size, karyopyknosis , cytoplasmic and nuclear chromatin condensation , density increased and a lot of apoptotic bodies among cell. Whereas some stromal cell displayed degeneration and necrosis. The protein expression of Fas and Caspase-3 in endometriotic tissue of XiaoChaiHu Decoction group was higher than that in the endometrium.

结果中、高剂量小柴胡汤（10，15 gkg-1d-1）治疗后，异位内膜有较多腺上皮细胞出现凋亡的特征，表现为细胞体积变小，核固缩，胞浆和核染色质凝集，密度增高，细胞间凋亡小体，同时间质细胞中可见一些坏死细胞；异位内膜Fas蛋白、Caspase-3蛋白的表达水平明显高于其在位内膜。

And the long axis of simple pit Varied along with CMfs orientation of each secondary wall.This paper proposed a structure model of Phyllostachys pubescens cell wall,based on the results of CMfs deposition and thickness of each secondary wall.Secondly, it was a multilamellate structure of parenchyma cell wall,but its thin and thick lamellate was not evident.CMfs orientation on primary wall of parenchyma cell was perpendicular to cell axis.

纤维的细胞壁为典型的薄厚层相间的同心圆多层结构，初生壁CMfs排列方向与细胞轴向垂直90°次生壁中厚层的CMfs均以接近平行的小角度(0～5°沉积；薄层一般以大角度沉积，且薄层的CMfs角度由壁外侧向细胞腔里逐渐增加，直到完全垂直。

The results show that when the cathode humidified temperature increase, the humidification of membrane and fuel cell performance improved at lower current density, while the saturation in diffusion layer increase and the performance decreases at higher current density. Humidification of membrane and fuel cell performance improved when the anode humidified temperature increase, the saturation in diffusion layer decrease and fuel cell performance improved when the fuel cell working temperature increase, the resistance of diffusion layer decrease and the performance improved when the porosity largen. At last, the simulation results are compared with the experimental results and they are basically the same.

结果表明随阴极加湿温度的提高，在低电流密度运行时膜的润湿条件改善，燃料电池性能提高，在高电流密度运行时扩散层中液态饱和度增加，燃料电池性能下降；随阳极加湿温度的提高，膜的润湿条件改善，燃料电池性能提高；随燃料电池运行温度的提高，扩散层中液态饱和度下降，燃料电池性能提高；随气体扩散层孔隙率增加，气体扩散层阻力减小，燃料电池性能提高。

Leucocytic differentiation antigen 40 sign-al access participates in regulation of inflammatory reaction accommodation of major cell component (vascular endothelial cell、vascular smooth muscle cell and macrophage), it is playing critical role in AS process, participates ather-osclerotic occurrence and development ,reside in atherosclerotic plaque cell thro-ugh CD40-CD40L interaction, induce activate by itself , express and secrete profit to happen immune response、inflammatory reaction、hemagglutination t-hrombogenic proteo-cytokine.

白细胞分化抗原40 (CD40)信号通路参与了动脉粥样硬化斑块内主要细胞成分(血管内皮细胞、血管平滑肌细胞以及巨噬细胞)炎症反应的调节，其在AS进程中扮演着关键性的角色，参与了动脉粥样硬化的发生和发展；存在于AS斑块内的细胞通过CD40-CD40L相互作用，导致自身活化，表达和分泌了有利于免疫应答发生、炎症反应、血凝和血栓形成的蛋白质细胞因子，如激活在AS斑块中的关键性细胞成分如黏附分子、炎症因子、基质金属蛋白酶等的产生，导致斑块的不稳定和破裂，而最终导致粥样硬化斑块病变的恶化。

Compared with the corresponding BEAS-2B cells, the shape of cell clone, the way of cell growth, the shape and quantity of cell and nuclear were not significantly changed in the 1st, 2nd, 5th passage BEAS-2BNNK cells. The shapes of cells and its nuclears of the 10th, 15th, 20th, 25th passage BEAS-2BNNK cells became round gradually, and the way of cell growth became from radio-form into membraniform and more than one nucleolus appeared.

BEAS-2BNNK细胞在第1、2、5代时细胞克隆形态及生长方式、细胞形态、细胞核形态及数目与相应代BEAS-2B无明显差异；第10、15、20、25代时细胞及胞核逐渐变圆，出现多个核仁，细胞由放射状生长逐渐变为膜状生长。

In the presence of linoleic platinum the ratios of W and S of breast cancer cell membranes and S180 cell membranes labeled with MSL were changed, The results indicate linoleic platinum can react with mercapto groups on the proteins of tumor cell membranes and change the conformations of cell membrane proteins.

亚油酸铂靶向脂质体的存在，使MSL的乳腺癌细胞膜和S180实体瘤细胞膜的W和S的比值发生了变化，结果表明亚油酸铂可以作用于癌细胞膜影响膜蛋白巯基结合部位，并使癌细胞膜表面蛋白质构象改变。

3Rhe distribution and change of proliferating cell nuclear antigen in seminiferous tubule: The positive staining of proliferating cell nuclear antigen was found in slices of all groups, the immunological positive products were located at karyon, usually in brown yellow granules, only a small part of them in diffuse state; the immunological positive cells were mainly the spermospores, besides a small quantity were spermiocyte, supporting the cell and myoid cell nuclear to be stained.

3增殖细胞核抗原在生精小管中的分布及变化：各组切片均可见增殖细胞核抗原阳性染色，免疫阳性产物位于细胞核，通常呈棕黄色颗粒，小部分呈弥散状；免疫阳性细胞主要是精原细胞，另外还有少量初级精母细胞、支持细胞和肌样细胞核着色。

Primary culture of bovine corneal endothelial cells and whole rabbit corneas were used as the experimental materials. We first treated the cultured bovine corneal endothelial cells with PTPs inhibitor, sodium orthovanadate, with a variety of concentrations (25,50,100μM) for various durations (8,24 hrs). The effects of PTP inhibition on cellular distribution of cell-cell junctional proteins, such as N-cadherin, alpha-catenin and p120, were evaluated by immunohistochemical staining with fluorescein microscopy and confocal microscopy. Immunohistochemical staining with Ki67 Ab, a marker of cell proliferation, was also used to detect cells entering cell cycle.

实验材料为初代培养之牛角膜内皮细胞和全厚度之兔子眼角膜，使用sodium orthovanadate作为酪胺酸磷酸脢的抑制剂，接著以不同SOV浓度(25， 50， 100μM)及不同时间(8， 24 hrs)来处理初代培养之细胞与新鲜的兔子眼角膜，之后进行萤光染色并使用萤光显微镜及共轭焦显微镜进行影像撷取，以观察位於细胞与细胞间的蛋白质，例如：N-cadherin， alpha-catenin 和p120的改变，另外也使用Ki67抗体来侦测是否有进入cell cycle的细胞。

The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构胚是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的，可在体外发育至孵化囊胚阶段，我们应用一种简便、快速的异种重构胚的鉴定方法，即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构胚的核遗传物质来源于大熊猫供核体细胞（后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性）。

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？