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Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组,并将阳性重组质粒,应用M13测序系统对产物进行DNA序列分析,并与MPM-129株P1基因核苷酸进行同源性比较,发现有4个位置的碱基发生了变化,其同源性为98.8%,证实了PCR所扩增DNA片段的准确性和特异性,同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异,将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针,杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应,再用BCIP和NBT呈色,制备MP DNA探针,鉴定所扩增片段的特异性,与同位素探针比较,Dig探针不受半衰期限制,可反复使用,而且价格低廉,值得推广使用。

Amino-6-methoxy Benzothiazole 99%、2-Amino Benzothiazole 99%、Veratraldehyde 99%、Veratric Acid 99%、1,2- Dimethoxy Benzene 99%、6-Nitroveratric Acid 97%、2,5-Dichlorobenzoic Acid 99%、4-Chloro-3-Nitrobenzoic Acid 99%、 3-Nitrobenzole Acid 98%、p-Methoxybenzoie Acid 99%、Anisole 99.5%、p-Methoxyacetophenonc 99%、(2'-Chloroethyl) Benzene 99%、(2'-Bromoethyl) Benzene 99%、N,N-Diethyi-m-Toiuamide 99%、Aminoacetonitrile Hydrochloride 98%、 Alkyl Diphenyl Phosphate Estrel 98%、5-Chloro-2-benzothiazolone 99%、6-bromo-2-naphthol 98%、6-Nitro-Veretric Acid Estrel 97%、4-chloro phenoxyaeetic 99

2-氨基-6-甲氧基苯骈噻唑、2-氨基苯骈噻唑、藜芦醛、藜芦酸、藜芦醚、6-硝基藜芦酸、2,5-二氯苯甲酸、3-硝基-4-氯苯甲酸、3-硝基苯甲酸、对甲氧基苯甲酸、苯甲醚、对甲氧基苯乙酮、β-氯代苯乙烷、β-溴代苯乙烷、N,N-二乙基间甲苯甲酰胺、氨基乙腈盐酸盐、磷酸二苯月桂酯、5-氯-2-苯骈噻唑酮、6-溴-2-萘酚、6-硝基藜芦酸甲酯、4-氯苯氧乙酸

Bis-(4-bromobenzoyl) benzene as the monomer of poly was synthesized by Friedel-Crafts benzoylation reaction. Poly-Ⅱ synthesized via palladium-catalyzed aryl amination reaction using 1, 4-bis-(4-bromo-benzoyl) benzene and 4, 4'-diaminodiphenyl ether as monomers.

通过傅-克酰基化反应合成了1,4-双-(4'-溴苯酰基)苯,以1,4-双-(4'-溴苯酰基)苯和4'4-二氨基二苯醚为单体,通过钯催化的胺基化缩聚反应合成了高性能聚合物-聚亚胺酮-Ⅱ。

Bromination of 4-chloropropiophenone with Br2 in AcOH gives a-bromo-4-chloropropiophenone in 98% yield.

乙酸中用液溴溴化对氯苯丙酮得到α-溴代对氯苯丙酮,收率98%。

The synthesis of l-(4-acetylaminophenyl)-2-bromo-1-propanone was discussed, and the mechanism and possible factors were analyzed. Hence the routine was determined, in which l-(4-acetylaminophenyl)- 1-propanone was reacted with Bromine dissolved in ethanol, so that the a -bromination can be carried on, resulted in the yield of up to 70.4%.

探讨了1-(4-乙酰胺基苯基)-2-溴代-1-丙酮的合成方法,分析了反应机理和影响因素,确定将1-(4-乙酰氨基苯基)-1-丙酮溶解在乙醇中以溴的乙醇溶液进行α-溴化的合成路线,产率可达70.4%。

Bromo-2′,4′-dichloroacetophenone was synthesized in three steps,w hich were bromization ,chlorination and acylation,from glacial acetic acid and m -dichlorobenzene.

以冰乙酸和间二氯苯为原料,经溴化、氯化、酰化三个步骤,制得 2—溴- 2′,4′-二氯苯乙酮,产率达 70 %。

Ethoxycarbonyl-4,5-dimethyl-pyrrole (7) was prepared from ethylacetoacetate, ethyl formate and methyl ethyl ketone via oximination, Claisen condensation, and reductive condensation. The bromization of compound 7 gave 2-ethoxycarbonyl-3-bromo-4,5-dimethyl-pyrrole(8), which could be transferred to 2-ethoxy-carbonyl-3-brome-4-methyl-5-formyl-pyrrole (9) by the formolation.

由乙酰乙酸乙酯、甲酸乙酯及丁酮等原料经过肟化、Claisen缩合、还原缩合成环得到2-乙氧羰基-4,5-二甲基-吡咯(7),溴化生成2-乙氧羰基-3-溴4,5-二甲基-吡咯(8),最后经过甲酰化反应得到目标产物2-乙氧羰基-3-溴-4-甲基-5-甲酰基-吡咯(9)。

The new synthetic technology of some important anthraquinone intermediates derived from 1-amino- anthraquinone, l-amino-4-hydroxy-anthraquinone, l-amino-4-bromo- anthraquinone-2-sulfonic acid, 1,5- and 1,8 dinitro- anthraquinone, have been studied.

本文研究了几种重要的由1-氨基蒽醌衍生的蒽醌型中间体:1-氨基-4-羟基蒽醌、1-氨基-4-溴蒽醌-2-磺酸和1,5-及1,8-二硝基蒽醌的合成化学,在此基础上开发了上述中间体的合成新技术。

The new synthetic technology of some important anthraquinone intermediates derived from 1-amino- anthraquinone, l-amino-4-hydroxy-anthraquinone, l-amino-4-bromo- anthraquinone-2-sulfonic acid, 1,5- and 1,8 dinitro- anthraquinone, have been studied.

本文研究了几种重要的由1-氨基蒽醌衍生的蒽醌型中间体:1-氨基-4-羟基蒽醌、1-氨基-4-溴蒽醌-2-磺酸和1,5-及1,8-二硝基蒽醌的合成化学,在此基础上开发了上述中间体的合成新技术。现行1-氨基-4-羟基蒽醌的生产技术是从1-氨基蒽醌出发,经二溴化再水解制得,溴的耗量在1.5mol以上。

Using 2-bromo-ethanesulfonic acid sodium salt ,α,ω- binary primary amine and dodecanoic acid as the main raw materials, drawing support from two-step synthesis of experiments—Alkylated reaction and Schotten-Baumann acylation, A series of LAS(12-s-12) gemini surfactants were synthesized.

并以2-溴乙基磺酸钠、α,ω-二元伯胺、月桂酸等为主要原料,借助胺基的烷基化和Schotten-Baumann酰基化反应制备了α,ω-烷撑-双钠盐LAS(12-s-12)表面活性剂,联结基团为亚甲基—(CH2)s—(S=2,4,6,8,12),取得以下进展

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