查询词典 bristle cells

We found that 1 mutant EG4 cells showed typical characteristics of pluripotent stem cells which had no obvious difference with wild cells; 2 When induced by 10〓 M retinoic acid , mutant EG4 cells differentiated into adipocytes with high frequency compared with mutant cells, suggested that EGFR plays a role in adipocyte differentiation; 3 when injected into nude mice, mutant teratocarcinomas contained a large amount of connective tissues as well as skeletal muscle, while wild EG4 cells produced frequently cartilage, keratinocyte and neuroepithelium.

我们建立了稳定表达胞内区功能缺失的外源EGFR cDNA片段的EG4细胞，分析其生长分化特性，发现 1）突变型细胞可在未分化状态下维持长时间的增殖，表明EGFR对EG多能干细胞表型无明显影响；2）〓 M的维甲酸A(retinoid acid A，RA)诱导后，大部分对照组细胞分化为脂肪细胞，而突变型细胞分化为脂肪细胞的比例明显较少，表明EGFR在脂肪细胞的发育分化中具有一定的调节作用；3）畸胎瘤切片分析显示，突变型瘤组织分布有大量的未分化细胞和结缔组织，分化细胞以肌肉细胞为主；对照组瘤组织含丰富的角质上皮、软骨、神经管等依赖EGFR的分化组织。

Under the regulation of different growth factors, they can differentiate into many kinds of cells, such as hepatocyte-like cells, cardiomyocyte-like cells, neuroglial cells, nerve cells, pancreatic islet-like cells and so on.

尽管如此，仍还不能将人羊膜上皮细胞称之为严格定义上的干细胞，因其不具有长期的自我更新和产生单个细胞克隆的能力，此外人羊膜细胞在体内分化成有功能的各种细胞类型需要进一步研究证实。

Methods DCs were prepared from peripherad blood mononuclear induce d with gra-nulocyt e/macrophage colony-stimulating factor and interleukin-4. Apoptosis of glioma cells were induced with γ-radiation. We design these experiment groups including (1) coculture of DCs and apoptotic glioma cells and T cells,(2) coculture of DCs and U937 cells and T cells,(3) coculture of DCs and cultured glioma cell and T cells,(4) coculture of Des and T cell.

用粒－巨噬细胞集落刺激因子加白介素－4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人脑胶质瘤细胞凋亡，将DCs、T淋巴细胞和凋亡胶质瘤细胞共培养，同时设计不同类型肿瘤细胞（U937及培养胶质瘤细胞）作对照，分离、富集DCs、T淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

Methods DCs were pre pared from peripherad blood mononuclear induce d with gra-nulocyt e／macrophage colony-stimulating factor and interleukin-4.Apoptosis of he patochlangioma cells were induced with γ-radiation.We design these experiment groups including(1)coculture of DCs and apoptotic cancer cells and T cells,(2)co culture of DCs and necrosis cancer cells and T cells,(3)coculture of DCs and cul tured cancer cell and T cells,(4)cocultu re of DCs and T cell,(5)cocuture of DCs and cultured cancer cell.

用粒-巨噬细胞集落刺激因子加白介素-4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人胆管癌细胞凋亡，将DCs、T淋巴细胞和凋亡胆管癌细胞共培养，同时设计不同类型肿瘤细胞(坏死胆管癌细胞及培养胆管癌细胞)作对照，7d后，分离、富集DCs、T 淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

Result: 1. The situation of HIV infection in gastro-mucosal tissues:(1) Our study found HIV gag sequence in not only CD4~+ T cells but also mucosalepithelial cells, gland epithelial cells in lamina propria and spindle stromal cells;(2) HIV p24 was expressed in T cells, plasmocyte, and a few mucosa epithelial cells, gland epithelial cellls and dell epithelial cellls.

结果：1.HIV在胃黏膜中的感染状况：(1)HIV感染者胃黏膜内HIVgag区基因不仅见于CD4~+T细胞等免疫细胞中，少数胃黏膜上皮、腺上皮、小凹内上皮细胞和间质梭形细胞中亦有阳性杂交信号；(2)HIV感染者尸检胃黏膜内HIVp24蛋白于T细胞、浆细胞及少部分胃黏膜黏膜上皮、腺上皮及小凹上皮细胞中呈阳性表达。2。

Results In 9 cases of endometrial biopsy of materials by uterine curettage, the cells of endometrial stromal sarcomas of 6 cases were well differentiated and like stromal cells of endometrial hyperplasia; the cells of endometrial stromal sarcomas of 3 cases were poor differentiated, the cells of 2 were like that of malignant lymphoma, and another like that of pleomorphous sarcoma. In 9 cases were found the distribution of vessels cluster like endometrial corkscrew small artery. The tumor cells were grew along lymphatic or protruded in lymphatic, and there were small islands or small pieces of tumor cells in the interspace of muscles in 2 cases. The contrastive observation of the specimens of resected uterines of 9 cases confirmed the valve of above features to the confessed diagnosis of endometrial stromal sarcomas in the materials of endometrial biopsy or uterine curettage.

有3例分化差者，其中2例酷似恶性淋巴瘤，1例酷似多形性肉瘤。9例均见簇状分布的子宫内膜螺旋小动脉样的小血管分布，2例见瘤组织沿淋巴管和突入淋巴管内生长及小片平滑肌组织的肌间隙内有小灶状或小片状瘤组织分布。9例子宫切除标本的对比观察，进一步证实了上述特征对宫内膜活检或刮出物确诊子宫内膜间质肉瘤的价值。

Comparing with control group, CD4~+ T cells of AIDS patient were markedly depleted in gastro-mucosal tissues(P＜0.01), but in presymptomatic patients CD4~+ T cells were not significantly different. CD3~+ T cells, CD8~+ T cells and NK. cells were notably increased (P.01), and they severely infiltrated glands and epitheliums in gastro-mucosal tissues. And the quantity and distribution of CD20~+ B cells between patients and control group were not obviously different.

HIV感染无症状者和AIDS患者胃黏膜活检组织中CD3~+T细胞、CD4~+T细胞、CD8~+T细胞、NK细胞、B细胞的数量及分布：与非HIV感染对照组相比，(1)AIDS患者胃黏膜内CD4~+T细胞显著减少(P＜0.01)，而无症状者与对照组无显著差异(P＞0.05)；(2)胃黏膜内CD3~+T细胞、CD8~+T细胞、NK细胞(CD57~+)显著增加(P＜0.01)，并呈现较明显普遍性噬胃黏膜上皮和腺体现象；(3)各组胃黏膜内CD20~+B淋巴细胞的数量分布变化不明显。3。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

After being induced to differentiate in vitro, cells with various morphologies showed β-gal activity, including nerve cells, neuroglial cells, epithelial cells. We also detected β-gal activity in a wide variety of tissue elements in solid tumors made by injecting the MC15 and MA34 cells into syngenic mice. Then we produced chimeric embryos by injecting the MC15 cells into Kunming blastocysts and recovering the embryos at various developmental stages.

SiCMVIE(Simian Cytomegalovirus Immediately Early)启动子是一个强启动子，本文首次将SCMVIE启动子应用到ES细胞途径上制作嵌合鼠，并通过lacZ基因在ES细胞和早期嵌合胚胎中的表达，表明该启动子是一个能在小鼠多数组织表达的启动子，这与1993年Peppel等人用原核显微注射法获得的转基因小鼠上所得的有关结果是基本上是一致的。

Careful inspection showed that the statue's head had been cast separately and soldered in place .

仔细检查发现雕像的头部是铸好后再焊接上去的。

Before clinic research, BB-83698 had exhibited the good antibacterial activity to a series of Gram-positive bacteria including several drug-fast bacteria.

在临床前的研究中BB-83698对一系列革兰氏阳性菌包括几种耐药菌都显示出良好活性。