查询词典 bristle cells

Methods: By the adherence , to isolate the bone marrow mesenchymal stem cells and adipose mesenchymal stem cells , to compare the characteristics of the two cells , including obtain the quantity of the cells 、 the morphos of the cells 、 the rate of adherence 、 cell kinetics 、 cell surface marker .

利用细胞贴壁性对骨髓间充质干细胞和脂肪间充质干细胞进行分离，比较两种细胞的情况，包括(1)单位质量组织获得两种细胞的量、(2)细胞形态、(3)细胞贴壁率、(4)细胞动力学、(5)细胞表面标记。

In order to study the effects of pre-heating on the sensitivity of cells to tumor necrosis factor-α, the K562 cells were pre-heated at 40℃ for different times to induce heat shock protein 70(HSP70). To observe the effects of TNF-α concentration on the sensitivity of pre-heated cells, cells were pre-heated for 60 min and treated with TNF-α(50,100,200 and 400U/ml) for 16 hours, and the cells vitalities were detected by MTT.

为探讨预热对肿瘤细胞肿瘤坏死因子α敏感性的影响及其机理，将白血病患者胸腔渗出细胞系(K562细胞)在40℃预热不同时间(0、30、60、90、120、150和180min)，使细胞热应激蛋白70(HSP70)高表达；然后将预热60min细胞和未预热细胞分别暴露于50、100、200和400U/ml的TNF-α16小时，用MTT比色法检测细胞活力。

Mechanism of oncolysis of Newcastle disease virus CN strain is studied in vitro in five carcinoma cells including HEP3B, T24, A549 and Hela cells, The pseudopods of the infected cells were retracted after 16h infected with 16HU/mL CN strain, the cells changed round and lost adhesion, the survival rates of the infected cells were lower than 10% by 120h.

本文研究了NDV-CN株对5株不同的人肿瘤细胞的体外杀伤作用。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性，通过建立猪皮肤成纤维细胞系，将所建细胞系与人胚胎肾293细胞体外共培养，并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验，结果表明，猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中，猪内源性逆转录病霉感染人胚胎肾细胞，进一步证实和拓宽了猪细胞PERV感染人细胞的范畴；猪皮肤成纤维细胞移植SCID鼠皮下后，导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官，但是并未检测出SCID鼠组织中表达PERV env RNA。

The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.

通过双向凝胶电泳分别分离TGIF转染细胞和PcDNA3.1空白质粒转染细胞的总蛋白质，测得两组细胞的蛋白质斑点数分别为760±41和680±38个；位置重复性分析表明TGIF转染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm；PcDNA3.1空白质粒转染细胞在IEF方向上的平均偏差为0.843±0.115mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。

METHODS: The subcutaneous adipose tissue was obtained from adult New Zealand rabbits under aseptic condition, and cultured in vitro with collagenase digestion. All cells were divided into 3 groups: in the BMP-2 group, cells were cultured with medium containing 0.1 g/L vitamin C, 10 mmol/Lβ-sodium glycerophosphate and 10μg/L BMP-2 for 10 minutes, followed by 4-14 days inoculation with density of 18×104 cells per pore. In the BMP-7 group, cells were cultured with BMP-7 with the same methods as BMP-2 group. The cells were cultured with simple culture medium in the control group.

无菌切取成年新西兰大白兔皮下脂肪，胶原酶消化法体外分离培养脂肪干细胞，分为3组：骨形成蛋白2组加入含0.1 g/L维生素C、10 mmol/L β-甘油磷酸钠、10 μg/L骨形成蛋白2的诱导培养基培育15 min，然后按18×104个细胞/孔接种，再培育4~14 d；骨形成蛋白7组培养方法基本相同，仅将骨形成蛋白2更换为骨形成蛋白7；对照组同法加入单纯培养基进行培育。

Nowadays, n stem-like cells have been found, which exist in the tumors of some tissues, such as haematogenesis system, brain, lung, mammary gland and so on. We defined them as tumor stem cells for their similar characteristics to the normal stem cells, but they are a minor population of tumor cells and the origin of them may be tumor cells.

随着对干细胞的研究不断深入，使人们对肿瘤的发生机制重新进行了审视，并在造血系统、脑、肺、乳腺等部位肿瘤中发现极少量的具有与干细胞非常类似生物学特性的细胞，称之为肿瘤干细胞，它们很可能是肿瘤细胞的起源。

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果：①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后，逐渐呈灰黄色，表面粗糙，出现裂隙；当压力逐渐减小后，变黄、粗糙、有裂隙的软骨颜色逐渐恢复，变得有光泽②光镜下可见表面光滑、平整，细胞分布均匀、整齐，层次清楚的软骨在关节面压力增大后，表面变粗糙、缺损，细胞排列紊乱、染色不均；当关节面压力逐渐减小后，软骨表面逐渐修复，细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达，术后3周内表达逐渐变弱，从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后，软骨细胞逐渐变扁，胞质中内质网膜、高尔基体减少，胶原排列紊乱；当关节面压力减小，软骨细胞形态逐渐恢复正常，胞质中内质网膜、高尔基体数量逐渐恢复；胶原纤维排列逐渐有规则。

Researchers have long known that in patients with Alzheimer's , the areas of the human brain clogged with senility-associated plaques also bristle with inflammatory cells and cytokines.

研究人员早就了解到，在患有老年痴呆症的病人中，被老年性斑块阻塞的大脑区域同时也充满了炎症细胞和细胞因子。

Furthermore, ed embryo exhibit extra C.N.S. and P.N.S. neurons. Ed also serves in Drosophila eye development and mesothoracic bristle development. Photoreceptor cells of the ommatidium and bristles are sensory nerves. We suggest that Ed might meditate the development of the nervous tissues.

此外，ed mutant胚胎有过多的中枢以及周边神经细胞生成，Ed亦参予在果蝇复眼发育以及背板刚毛的形成过程，果蝇复眼感光细胞以及刚毛均为周边神经细胞，显示Ed对於神经细胞生成具有某种程度的影响。

During the conversation,we introduced the inner concept of SIFE SZU and described the project about undergraduate employment.

在与两位教授的谈话中，我们向其介绍了SIFE深圳团队的核心理念，并描述了本次有关大学生就业项目。

The mountains and its plant region form its distributionbackbone,the heat and water condition in winter is one of the important factors and thetopography and geomorphy makes the distribution patterns complicated.

1在秦岭、淮河以北地区：植物区系的空间变化奠定了区域内森林群落植物物种多样性为纬向方向的空间变化格局。