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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。

Strabismus diorthosis under microscope has clear advantages compared with traditional strabismus diorthosis. The muscle, blood tube and the move of needle in sclera as well as the depth can be seen clearly during the operation which make the operation easy to control, so we can properly and correctly perform the operation, shorten the operation time and avoid complications like blooding, penetrate sclera which often occur during the traditional operation. The strabismus diorthosis under microscope using general micro equipments can also separate structure correctly with fewer operation steps, light reaction after operation, sooner recovery after operation, which is suit to patient who needs strabismus diorthosis.

显微镜下斜视矫正术较传统直视下斜视矫正术具有较明显优势,可以清晰地看清肌肉、血管、及针在巩膜内的走行、深浅,使手术较易掌控,可以使手术量及手术操作准确无误,缩短手术时间、避免出血、穿透巩膜等直视下手术常见的并发症的发生,同时组织分离准确,操作减少,也直接减轻了术后反应,缩短了术后恢复时间,适合所有需斜视手术患者,优势为初学者易掌握巩膜进针深度。

To sb's notice bring/call to mind bring to a halt bring into action catch one's breath call down / lay a curse upon sb. cast a shadow over catch at shadows catch sb's eye catch fire catch / get a glimpse of catch sight of come to sb's knowledge come to sb's notice come to a point come /go into effect come into a fortune come into fashion come to a halt combination with commander in chief communication with commit a crime commit / make an error come into possession of sth. come / go into operation come to one's senses come to / arrive at a decision come to / arrive at an agreement with reach / make an agreement with come to sb.'s assistance complaint about/of competition with / against sb.

do a threat to do damage to do/try one's best 与----接触不平衡与-----保持友好与----一致对----负责违背诺言实施,使生效给某人带来羞辱使某人注意----想起使停止采取行动喘息诅咒某人蒙上阴影捕风捉影引人注目着火,烧着对----瞥一眼看见被某人知道引起某人的注意到紧要关头实施,生效继承一笔财产开始流行停止与------结合总司令与-----交流,与----通信犯罪犯错误占有某物实行,生效恢复理性,苏醒过来做出决定与-------达成一致与-------达成一致帮助某人对------抱怨与某人竞争对----有威胁损害-----尽力,努力

"May I bring, may I bring,may I bring you a salad?May I bring,may I bring you a salad?Would you like a cup of coffee?How about some herbal tea?May I bring you some dessert?"

贸易梯梯TT外贸学院,有权但无此义务,改善或更正所刊登信息任何部分之错误或疏失。

This article starting from comparing separating-operation with multi-operation analyzes the reasons of international baking"s multi-operation in the view of institution economy, in the base of which discusses the necessities, possibilities and significances of our commercial banks" transforming from separating-operation into multi-operation under the background of international bakings multi-operation trend.

本文以国际银行业综合经营趋势为背景,从比较商业银行分业经营与综合经营的利弊入手,从制度经济学的角度分析了国际银行业综合经营的原因,并在此基础上论述了我国商业银行由分业经营向综合经营模式转变的必要性、可能性及意义。

During process of dynamic operation system integration centering on operation tasks,because of complexity of the logic relations among operation tasks and limited number of operation system s,there may exists operation system s conflicts among operation tasks.

以作战任务为中心,动态集成作战系统的过程中,由于作战任务间逻辑关系的复杂性,以及作战系统的数量有限性,作战任务之间可能存在作战系统冲突问题。

For eliminating the hidden danger caused by the misiperation in the present operation law and flow, the system will manage the operation rule and the operation course by the computer, at the same time verify the validity of every operation step of every course to avoid operation mistake as a result of the carelessness of operator or the change of the operation mode.

本文所建系统针对供电公司现有操作制度和工作流程中的隐患,利用科学的算法和规则进行安全校验,避免了电网操作过程中由于人的疏忽和电网的变化而引起误操作的发生。

In the traditional reservoir operation system, in general, we use the rule curve as a method of operation, and the reservoir rule curve is the result of system simulation method. Due to the regular and simple rule curve operation unable to satisfy the actual demand effectively. In order to improve the defect, there are many system planning theories and optimum seeking methods to find the optimum operation strategies. The reservoir operators are uncomfortable with the complicated optimum operation strategies. In this article the fuzzy inference model is used to establish a simple and effective operation rule for the reservoir operator.

在传统的水库操作系统中,一般都使用操作规线为操作方式,水库操作规线是系统模拟法所获得的结果,由於规律、简单的规线操作方式往往无法有效满足实际需求,为了改善传统操作上的缺点,近多有利用系统规划之理论,应用各种优选方法求取最佳操作策略,系统最佳操作策略其模式复杂,水库操作人员不易接受,故本文即以模糊推论系统建立一简单有效之操作规则予水库操作人员,作为水库操作放水之依据。

Methods To analyze 28 cases with thyroglossal duct cyst and fistula (7 cases of recurrence after operation). The infected cases were treated with antibiotic first before operation, and judged the relationship between thyroglossal duct cyst and hyoid bone exactly through checking and sonography, and then to deal with hyoid bone. First, we should inject methylthioninium chloride in the auditory tube to trace one day before the operation or during the operation, with Sistruk operation, and separate the little branches during operation, handling the focus of infection with radio frequency and microwave or armillary transfixion and so on.

回顾分析28例甲状舌管囊肿病例(含7例术后复发者),术前感染病例先行抗炎治疗,通过查体及B超检查,准确判定甲状舌管囊肿与舌骨关系,处理舌骨;术前一天或术中先注亚甲蓝液入囊管内示踪,行Sistruk术式,仔细分离分支,手指触诊疑为残存病灶处,行环形缝扎或射频、微波等处理。

In autumn, always bring people pleased celebrate, bring poetry, bring fancy, bring to imagine, bring a hope more, but this year's autumn, not- same sort, more and not and usually.in this fine season, arrive to°from chiang-nan water country a northland backland, from the gan river strand to pull sa lin ka, hot soils of republic each square kilometer soak through exultant and happiness, flow to drip sweet with sing.1,300,000,000 china childrens with heroic the singing arousing more put to fly aureate ideal and imagining of rose and put to fly hard go such as the conviction of huge rock and the poem lauding to the great motherland.

秋天,总给人们带来喜庆,带来诗意,带来遐想,带来憧憬,更带来希望,而今年的秋天,非同一般,更不寻常。在这美好的季节,从江南水乡到北国边陲,从赣江只滨到拉萨林卡,共和国每一平方公里的热土都浸透着欢欣与幸福,流淌着甜蜜与歌唱。十三亿华夏儿女以豪迈激越的歌声放飞金色的理想与玫瑰色的憧憬,放飞坚如磐石的信念与对伟大祖国赞美的诗行。

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推荐网络例句

I'm going tor her!

我这就去救她!

As rise are design to help keep a several toney , a brain chemical at continues lever.

SSRI被设计来帮助保持血液中的复合胺即在大脑中一个连续水平的化学物质。

So I'm going to give you two easy tests.

所以,我给你们做两个简单的测试。