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In one group oocytes were cultured for 3h and in the other one for 6h. Incidence of germinal vesicle breakdown was observed following the culture with BL-Ⅰ.

BL-Ⅰ培养液培养结束时,观察卵母细胞生发泡破裂的发生率,再用不含BL-Ⅰ的培养液继续培养,进一步观察其成熟动力学的变化,即卵母细胞GVBD过程和第一极体形成过程。

The results showed that chromium trichloride could inhibit the extruding of the first polar body and affect the quality and the viability of mouse oocyte and reduce the rate of IVF and the number of oocytes of superovulation, inhibit the germinal vesicle breakdown of mouse oocytes in vitro culture, but little impact on GVBD of mouse oocytes in vivo .

结果表明,三氯化铬可以抑制卵母细胞第一极体的释放,降低小鼠超排卵数和卵母细胞的存活率和体外受精率。

Interaction between MAP kinase and MPF influerlces meiotic maturation and fertilization of oocyte throughout the animal kingdom, including stimulation of germinal vesicle breakdown, suppression of DNA replication, control of meiotic chromosome segregation, maintenance of metaphase Ⅱ arrest, and resumption and completion of second meiosis.

方法分别用MAPK激酶特异性抑制剂(U0126)和MPF特异性抑制剂抑制受精卵细胞MAPK和MPF的活性,通过同位素测定MAPK和MPF的活性变化,利用显微镜进行形态观察来判定MAPK活性变化对受精卵发育的影响。

RESULTS: The rates of germinal vesicle breakdown were 98%, 22.2%, 40.1% and the rates of the first polar body (PB1) extrusion were 77.7%, 15.5%, 27.6% for M16, Hx and FA groups, respectively. The rates of GVBD and PB1 were all increased with the addition of FA compared with the Hx groups and comparison was significant (P.05). Hx and FA did not influence chromosome, but FA reversed the size of metaphase spindle elongated by Hx.

结果:M16培养液组、Hx模型组和FA处理组的GVBD率分别为98%、22.2%、40.1%,其PB1排出率分别为77.7%、15.5%、27.6%,各组间的差异均具有统计学意义(P.05);次黄嘌呤和叶酸对染色体的形态没有影响,而次黄嘌呤使正常的纺锤体变长、变宽、面积变大,FA组较Hx模型组增大的纺锤体有所恢复,并接近于正常水平。

PKA activators such as cAMP, 8 - Br -cAMP or phosphodiesterase inhibitor isomethyl butyl xanthine or purified PKA catalytic subunit all can inhibit germinal vesicle breakdown and meiotic maturation in mouse oocytes, also in Xenopus oocytes.

以小鼠卵母细胞为实验对象,给予PKA激动剂cAMP、8-Br-cAMP或磷酸二酯酶抑制剂IMBX均可抑制小鼠卵母细胞的胚泡破裂及减数分裂成熟。

This device can measure frequency,electric voltage and variety rate quickly, distinguish shortcircuit breakdown, judging the size of power deficiency vacancyinside a system.

一旦电力系统出现不稳定它能快速切除接近于功率缺额的负荷,抑制系统电压频率的快速降低,保证电网安全并保障一些重要用户的供电质量。

Some of the hard disk LSI circuit is made of MOS technology, MOS circuits are particularly sensitive to static electricity, electrostatic induction was susceptible to breakdown damage, so pay attention to the issue of anti-static.

硬盘电路中有些大规模集成电路是MOS工艺制成的,MOS电路对静电特别敏感,易受静电感应而被击穿损坏,因此要注意防静电问题。

Joint damage called osteoarthritis, also known as degenerative joint disease is manifested by cartilage and bone breakdown and irregular bony remodeling in response to stresses and inflammatory processes in the joint.

髋关节损坏称为骨关节炎,也被称为关节变性病,它被证明在经重力和激烈性运动,其临床表现症状为髋关节结合出软骨病,骨质疏松和骨骼异常生长。

Agents can also cross-link each hemoglobin molecule internally to prevent its breakdown into dimers.

一些双功能试剂也能使每个血红蛋白分子内部交联,以防止分解成二聚体。

The breakdown of the human corpus luteum vary harm, clinical symptoms and manifestations are also very different.

黄体破裂对人的危害因人而异,临床症状及表现也有很大差别。

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