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TheMADS box locates just at the 5'end of most of genes and is highly conserved.According to these,a primer was designed and the effort to isolate new membersof MADS box gene family in rice genome by RT-PCR was made.

绝大多数已克隆到的这类基因的MADS-box恰好位于5'端，而且非常保守，因此，我们根据MADS box基因的保守区设计了一个特异的引物，试图通过RT-PCR分离出水稻花中MADS box基因家族的新成员。

Further bioinformatical analysis indicated that the three Y-box genes of lamprey, i.e., Lyb1, Lyb2, and Lyb3, encoded 331, 181, and 171 amino acids, respectively. The three amino acid sequences were homologically aligned with those from Ciona intestinalis, Danio rerio, Oryzias latipes, Takifugu rubripes, and Mus musculus by DNAMAN software.

采用DNAMAN软件，将七鳃鳗的3个Y-box蛋白氨基酸序列与海鞘、斑马鱼、清鳉、河豚鱼和小鼠Y-box蛋白进行同源性分析，结果显示他们共有一个保守的冷休克结构域，其中包含两个RNA结合基序RNP-1和RNP-2，表明获得的Lyb基因属于Y-box 基因家族成员。

CAAT box" and "TATA box, potential regulatory motifs from transcription, and conserved cis-acting regulator elements were discovered within LTRs in the great number of sequences using plantCARE sortware.6 The insertional polymorphism of LTR-10 element within the genome of 8 wild species and 28 cultivars of Mallus Mill. was studied by sequence-specific amplification polymorphism.

分析获得的Tyl-copia类逆转座子的LTRs，其中含有启动子的结构特征"CAAT box"和"TATA box"及受不同胁迫条件作用的调控元件。6、基于苹果Tyl-copia类逆转座子LTR-10的SSAP技术在苹果属8个野生种和28个栽培品种中表现出了丰富的遗传多态性，多态性片段比例为88.2％。

So it is assumed that the E-box might be associated with the enhancement of transcription activity.Here, the LTRs of WH17 ,DLA-25,DLA-118 and LTR with single mutation in the E-box motif were separately cloned into pCAT-Basic vector resulting in a series of recombinant plasmids containing CAT reporter under the control of different versions of LTRs.

通过对EIAV分离株WH17的LTR与L株、DA和DLA株的LTR序列进行比较，发现其在在U3-R结合处多一个E-box基序，推测该基序的变化可能会起到促进转录作用，为此将EIAV强毒株(DLA-25)、驴白细胞弱毒株(DLA-118)、EIAV分离株WH17以及U3-R结合处的E-box基序点突变的LTR分别克隆到pCAT3-basic质粒中的报告基因氯霉素乙酰转移酶的上游，获得一系列受不同LTR控制的CAT表达质粒。

The ubiquitin proteolytic system is an important selective proteolytic system of the celler proteolytic systems, a number of them were identified as a ubiquitin chain assembly factor-E4. The U-box protein is a new family ubiquitin-protein ligase(E3), which determine the substrate specificity of ubiquitination. The U-box is a domain of 70 amino acids that is highly conserved among yeast, plant and animals, but more U-box proteins were found in plants than in animals.

泛素系统是选择性降解细胞内蛋白质的重要系统之一，U-box蛋白质是此系统中决定底物特异性识别的一种新型E3蛋白质，部分U-box蛋白质属于泛素链聚集因子-E4.U-box结构域大约由70个氨基酸残基构成，在酵母、植物和动物等真核生物中保守存在，但植物中的数目远多于动物中。

At a glance of the molecular mechanism, one gene encoding 218 amino acids was identified from cDNA-AFLP differential study and then named as Ipomoea batatas MADS-box 1 gene (IbMADS1) based on earlier studies. From sequence alignment and phylogenic analysis, IbMADS1 exhibited a typical type II-MIKC structure, including two conserve domains, MADS-box and K-box.

本论文针对从甘薯块根筛选出的MADS-box基因，IbMADS1（Ipomoea batatas MADS-box gene 1）进行分析，分别探讨该基因在甘薯各部位的表现方式、受植物荷尔蒙调节的影响，以及甘薯属中野生亲缘种间的基因表现歧异性，再进一步从转殖植物的外表形态了解此基因可能的生理功能。

The analyses indicated that the upstream sequence contained several regulatory elements,such as CCAAT-binding factor,GC-box,HSE and GATA,but TATA-box and CAAT-box were not be found.

分析表明，该上游序列的转录起始位点位于第3255位G碱基处，没有明显的TATA-box和CAAT-box，但含有CCAAT-binding factor、GC-box等重要的转录因子结合位点，以及热激应答元件HSE和GATA元件等启动子顺式调控元件。

The Lyb genes were novel members of Y-box gene family containing a conserved cold-shock domain with two RNA binding motifs (RNP-1 and RNP-2). Moreover, phylogenetic analysis revealed that there was only one type of Y-box gene before the emergence of gnathostomata. By contrast, the Y-box gene were divided into three groups, YB1, YB2, and CSDA during evolution of jawed vertebrates.

此外， 从Swiss-Prot下载了经实验验证过的Y-box蛋白序列数据，结合实验克隆得到的Lyb基因所编码的氨基酸序列构建系统发育树，发现Y-box基因在有颌类出现以前只有一种类型，有颌类出现以后， Y-box基因分化成YB1、YB2和CSDA三种类型。

One of these clones,M79 was selected for further study.M79encodes a typical MADS box protein,and it shares high homology with many ofthe MADS box genes in plants.

对其中一个克隆M79的DNA序列和蛋白质序列分析表明，M79基因编码多肽含有一个典型的MADS box和K-box，与植物中的许多MADS box基因产物有很高的同源性。

Polymorphism of HLA-DQB1 promoter region in Hans IDDM patients and normal controls have been identified by PCR, PCR/SSCP and PCR/sequencing methods.No differences were found in y and s box between patients and controls carrying different allele as well as in different ethnic groups. There are two different sequences in x box,but CCTAGAGACAGATT sequence locates frequently on the haplotype with DQB1.0302 allele. Polymorphism between transcription point and y box (at position －44～-46 and －59～-61) might be associated with the genetic susceptibility to IDDM. Additionally,a new single base mutant (CACC→CAC A ) was found at position －131 and －128 in two patients carrying DQB1.0601 allele.

结果显示携带不同等位基因的患者与对照者DQB1 5'-调控区y、s box核苷酸序列相同，且与白种人基因结构一致；y box核苷酸序列存在二种结构，CCTAGAGACAGATT序列常常与DQB1.0302等位基因在同一单倍型；转录起始位点至y box间-44至-61位存在多态性，-59至-61位AAG等位基因可能与1-型糖尿病易感相关联；在2例携带DQB1.0601等位基因患者的-131至-128位间发现CACC→ACA A单个碱基取代突变。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。