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TheMADS box locates just at the 5'end of most of genes and is highly conserved.According to these,a primer was designed and the effort to isolate new membersof MADS box gene family in rice genome by RT-PCR was made.

绝大多数已克隆到的这类基因的MADS-box恰好位于5'端，而且非常保守，因此，我们根据MADS box基因的保守区设计了一个特异的引物，试图通过RT-PCR分离出水稻花中MADS box基因家族的新成员。

If the user choose to hope that the new program and the guidelines of the program or system in order to bring the name of your favorite preservation, may be in the "Program" drop-down list box, select the target program, and then click "Save As" button, open the Figure 3 - 3 shown in the "Save the program" dialog box, in the "Save the program the cursor to" text box to save the new name, and then click "OK" button close the dialog box.

如果用户希望新选择的指针方案和或系统自带的方案以自己喜欢的名称保存，可在"方案"下拉列表框中选择该指针方案，然后单击"另存为"按钮，打开如图3 - 3所示的"保存方案"对话框，在"将该光标方案另存为"文本框中输入要保存的新名称，然后单击"确定"按钮关闭对话框。

The inner box is arranged inside the outer box in a sliding measure. Wherein the inner box comprises three individual modules as battery containing module which can be used for containing a battery, an inserting module which can be used for the battery in the battery containing module conducting connecting outside the outer box, and an attachment module in which a battery managing system that is used for monitoring the working state of the battery can be arranged.

其中所述内箱体包括三个独立的模块，分别是：电池容放模块，电池容放在所述电池容放模块中；插接模块，所述电池容放模块内的电池通过所述插接模块导电地连接到所述外箱体；以及附件模块，其中设置有用于监控所述电池容放模块内的电池的工作状态的电池管理系统。

Unit 4 "lady hermits who are down but not out"中写到once a shopping-bag lady becomes a figure of your neighbourhood, it is as hard to pass her by without giving her some money as it is to ignore the collection box in church.

"在中国的文化传统中，人们经过教堂或寺庙里的募捐箱时不往里面投钱也无可厚非。可是在西方许多国家，由于信教的人数众多，人们一般不会认同对"collection box的漠视。

The ubiquitin proteolytic system is an important selective proteolytic system of the celler proteolytic systems, a number of them were identified as a ubiquitin chain assembly factor-E4. The U-box protein is a new family ubiquitin-protein ligase(E3), which determine the substrate specificity of ubiquitination. The U-box is a domain of 70 amino acids that is highly conserved among yeast, plant and animals, but more U-box proteins were found in plants than in animals.

泛素系统是选择性降解细胞内蛋白质的重要系统之一，U-box蛋白质是此系统中决定底物特异性识别的一种新型E3蛋白质，部分U-box蛋白质属于泛素链聚集因子-E4.U-box结构域大约由70个氨基酸残基构成，在酵母、植物和动物等真核生物中保守存在，但植物中的数目远多于动物中。

Most genes in the ABC model of the development of plant flowers in hermaphroditic plants belong to MADS-box genes. But the researches on MADS-box genes in dioecious plants are less.

雌雄同株植物花发育的ABC模型中已知的大多数基因都属于MADS-box基因，但在雌雄异株植物中对MADS-box基因的研究较少。

In this review, we use Arabidopsis thaliana and Oryza sativa as examples, and summarize the study results of floral homeotic MADS-box genes in two major groups of angiosperms — core eudicots and monocots — over the past decade. Our aim was to review the functional conservation and diversity of floral homeotic MADS-box genes in angiosperms and discuss whether the ABCDE model has been conserved in monocotyledons.

本文以模式植物拟南芥和水稻为例，综述了近10年来对被子植物两大主要类群——核心真双子叶植物和单子叶植物花同源异型MADS-box基因的研究成果，分析MADS-box基因在被子植物中的功能保守性和多样性，同时探讨双子叶植物花发育的ABCDE模型在多大程度上适用于单子叶植物。

One of these clones,M79 was selected for further study.M79encodes a typical MADS box protein,and it shares high homology with many ofthe MADS box genes in plants.

对其中一个克隆M79的DNA序列和蛋白质序列分析表明，M79基因编码多肽含有一个典型的MADS box和K-box，与植物中的许多MADS box基因产物有很高的同源性。

Polymorphism of HLA-DQB1 promoter region in Hans IDDM patients and normal controls have been identified by PCR, PCR/SSCP and PCR/sequencing methods.No differences were found in y and s box between patients and controls carrying different allele as well as in different ethnic groups. There are two different sequences in x box,but CCTAGAGACAGATT sequence locates frequently on the haplotype with DQB1.0302 allele. Polymorphism between transcription point and y box (at position －44～-46 and －59～-61) might be associated with the genetic susceptibility to IDDM. Additionally,a new single base mutant (CACC→CAC A ) was found at position －131 and －128 in two patients carrying DQB1.0601 allele.

结果显示携带不同等位基因的患者与对照者DQB1 5'-调控区y、s box核苷酸序列相同，且与白种人基因结构一致；y box核苷酸序列存在二种结构，CCTAGAGACAGATT序列常常与DQB1.0302等位基因在同一单倍型；转录起始位点至y box间-44至-61位存在多态性，-59至-61位AAG等位基因可能与1-型糖尿病易感相关联；在2例携带DQB1.0601等位基因患者的-131至-128位间发现CACC→ACA A单个碱基取代突变。

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。