查询词典 bovine

METHODS: Lipofectamine method: The BMMSCs were obtained from the tibias and femurs of the guinea pigs. The third passage BMMSCs were cultured with plasmid-lipofectamine mixture for 6 hours, followed by fetal bovine medium for 48 hours. Immunohistochemistry was performed for transient expression. G418 was added after 48 hours.

①脂质体法：取体外分离、培养的第3代豚鼠骨髓间充质干细胞，将质粒-脂质体混合物加入含细胞的培养基中培养6 h，再加入胎牛血清的培养基，孵育48 h 后行免疫组织化学检测，即为瞬时表达。48 h后加入含G418培养基筛选。

The practical experiences of several tannages and my own tanning experiences were combined to summarize the producing and dyeing technology of suede split bovine leather .

介绍了牛二层绒面革工艺技术和染色方法，在实际生产过程中，借鉴各家工厂生产牛二层的经验并结合个人试验实践，总结出牛二层绒面革的加工及染色工艺。

With the increase in fetal bovine serum concentration used in the culture, greater expansions in total cells and granulocyte-macrophage colony-forming-units were observed, and it was found that CB plasma synergized with FBS to support the growth of CB MNCs in liquid and semisolid cultures.

血清是细胞培养中的重要添加物。随着胎牛血清浓度的增加，促进总细胞和粒细胞巨噬细胞集落形成单位扩增的能力增强。脐血浆和FBS具有很好的协同作用。

" And influence and the element that restrict economic benefits are many sided, but affect beneficial result " bovine nose the key is quality.

&而影响和制约经济效益的因素是多方面的，但牵动效益的&牛鼻子要害是质量。

Methods The guts of embryonic mice removed and dissociated were plated into serum-free DMEM/F12 medium. The mitogen-free DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs. Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.

分离胚鼠肠管，消化后接种于添加N2、B27和碱性成纤维细胞生长因子的DMEM/F12培养基，贴壁培养；连续传代后用血清促进GNCSCs分化；最后用免疫细胞化学方法染色鉴定。

Methods The guts of embryonic mice removed and dissociated were plated into serumfree DMEM/F12 medium. The mitogenfree DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs. Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.

分离胚鼠肠管，消化后接种于添加N2、B27和碱性成纤维细胞生长因子的DMEM/F12培养基，贴壁培养；连续传代后用血清促进GNCSCs分化；最后用免疫细胞化学方法染色鉴定。

Key words: bovine IgG2 Fc receptor; COS-7 cells; cell surface expression; rosetting assay

牛IgG2 Fc受体；COS-7细胞；细胞表面表达；玫瑰花环试验

Kir4.1 and Kir7.1 acomplished by ABC immunohistochemistry in monkey and human retinae got the nearly same results as bovine .

ABC免疫组织化学显示Kir2.1和Kir4.1分布在猴和人视网膜 M沮ler细胞的不同部位，而Kir7.1分布于人和猴的RPE细胞的游离面及其突起。

Channel subunits, nearly same property as adults at P16 murine retinae, is in müller cells, not in RPE. There is no difference between species. The Kir7. 1 channel subunit, nearly same property as adults at P7 murine RPE, is a major component of the apical K〓 conductance in bovine RPE.

这些结果显示，Kir2.1和Kir4.1在P〓接近成年水平，分布于视网膜Müller细胞的不同部位，在不同种类之间无明显差别，这种分布特性可能与完成Müller细胞功能-保持细胞外K〓的平衡，促进K〓的内流有重要关系。

No immunostaining was seen in RPE. Double staining that Kir2. 1 and glutamine synthetase, Kir2. 1 and glutamine synthetase colocalized well. Intense Kir7. 1 immunolabeling was present on the apical surface of all RPE cells and appeared to extend over the length of the apical processes. Na〓, K〓-ATPase expression varied among RPE cells, but in highly expressing cells, it co-localized with Kir7. 1. Immunoreactivity of Kir2. 1、Kir4. 1 and Kir7. 1 acomplished by ABC immunohistochemistry in monkey and human retinae got the nearly same results as bovine.

间接免疫荧光组织化学显示，Kir2.1蛋白主要分布在Müller细胞与神经元相接触的细胞膜区域；与Müller细胞的特异性标记蛋白质抗谷氨酸合成酶抗体双重标记显示其分布特性重叠，在RPE未检测Kir2.1蛋白的免疫活性存在；Kir4.1蛋白集中分布于Müller细胞的内侧突起，与GS蛋白双重标记显示其分布特性重叠，RPE未检测Kir4.1蛋白的免疫活性存在；Kir7.1主要分布于RPE游离面及其突起的全长，与特异性标记RPE突起的Ezrin蛋白完全重叠，Na〓-K〓ATP酶在不同部位的RPE表达不同，Na〓-K〓ATP高表达的RPE细胞与Kir7.1的分布特性重叠。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。