查询词典 bovine

ES cells were maintained on gelatin-coated dishes in Iscove's modified Dulbecco medium containing 0.1 mmol/L of nonessential amino acids, 2 mmol/L of L-glutamine, 0.1 mmol/L of monothioglycerol, 15% fetal bovine serum, and 1000 U/mL leukemia inhibitory factor. Embryonic bodies were firstly formed by ES cells with hang drip method in IMDM without LIF. Six-day-old EBs were trypsinized and the cells were resuspended at a concentration of （3-4）×109 L-1 cells per 1 mL of a 1:1 mixture of culture medium and Matrigel?

实验方法：将鼠胚胎干细胞株接种于被覆明胶的培养瓶内，加入含0.1 mmol/L非必需氨基酸、2 mmol/L L-谷酰胺、0.1 mmol/L二羟基丙硫醇、15%胎牛血清、1 000 U/mL白血病抑制因子的IMDM培养液。3 d后采用&悬滴法&使细胞在不含白血病抑制因子的上述IMDM培养液中形成胚胎体，6 d后胰酶消化得到胚胎干细胞，浓度调整为（3~4）×109 L-1，与Matrigel？

Henan Lotus Group's production of "Lotus" brand MSG has won 24 domestic and international gold medal, its production capacity among the world's top four in same industry, single-plant output ranks first in the world; Song River wine production of high and low degree of Song River Grain Liquid Wine won national gold medal and entered the ranks of the national wine; Henan Shoes City Group's production of bovine leather and leather products are exported to more than 10 countries and regions.

河南莲花集团生产的&莲花&牌味精先后获得24块国内国际金奖，其生产能力跻身世界同行业四强，单厂产量居世界第一；宋河酒业厂生产的高、低度宋河粮液酒获国家金奖，进入国家名酒行列；河南鞋城皮革集团生产的牛皮革及制品远销10多个国家和地区。

This thesis compared the effect of Uracil, ITS, and ATP on the maturation of bovine oocytes and further development, observed the microstructure changes of oocytes and parthenogenetic embryos among different groups, and granule cell apoptosis among different groups.

本文对比了添加尿嘧啶和三价因子等试剂对牛卵母细胞成熟及后期发育的影响、观察了不同组之间成熟牛卵母细胞和孤雌激活胚胎显微结构变化和不同组之间颗粒细胞凋亡情况。

Using an anticoagulant it was possible to isolate plasma and intact haemocytes from Helicoverpa armigera larvae. PO and ProPO activities in plasma and haemocyte lysate were assayed when using bovine trypsin as an activator and L-DOPA as a substrate.

用抗凝剂从棉铃虫血淋巴中分离获得了血浆及完整的血细胞，以L-DOPA为底物，牛胰蛋白酶为激活剂，测定了血浆及血细胞裂解液中酚氧化酶及酚氧化酶原的活性。

Methods Hepatic cells were obtained from fetal rats by means of mechanical separation and hanging-drop culture, and after two days of primary culture, the medium was changed for further cell culture in the presence of 20% fetal bovine serum (containing glucose 25ml/L, mycillin 100 U/ml, pH 7.6), 10 mmol/L nicotinamide, 1 mg/L insulin, affix N2, basic fibroblast growth factor, stem cell factor, epidermal growth factor and leukemia inhibitory factor.

采用机械分离法结合悬滴培养法获得胎肝干细胞，原代培养2d后改变培养基，添加含20%胎牛血清的DMEM（含25ml/L葡萄糖，100U/ml青链霉素，pH值7.6），添加烟酰胺10mmol/L，胰岛素1mg/L，N2添加剂，碱性成纤维细胞生长因子、表皮生长因子、干细胞因子、白血病抑制因子各20μg/L诱导分化为nestin阳性细胞。

Class-specific polyclonal antibodies for O,O-diethyl phosphorothioate pesticides had been generated. A series of generic haptens were synthesized and coupled to bovine serum albumin and ovalbumin for immunogens and coating antigens, respectively. New Zealand rabbits were immunized with immunogens to obtain corresponding polyclonal antibodies.

通过分析硫代磷酸二乙酯类农药的结构特点，设计并合成了系列半抗原；采用活泼酯法将半抗原分别与牛血清蛋白和卵清蛋白偶联制备了系列免疫原和包被原；通过免疫新西兰大白兔获得了相应抗硫代磷酸二乙酯类农药的类特异性抗体。

Then, the hapten LBc and LBs was conjugated to bovine serum albumin with CDI method to form immuno-antigen; the haptens LBc, LBs and LBy was conjugated to ovalbumin with mixed anhydride method to form the the coating antigens.The new zealand rabbits were immunized by conjugate of LBc-BSA and LBs-BSA, and titres of anti-bifenthrin serum (5.12×10~4, 3.2×10~5) were determined by non-competitive indirect enzyme-linked assay procedure.

通过碳二亚胺法将LBc和LBs交联于牛血清蛋白作为免疫抗原，通过活泼酯法将分别LBc、LBs和LBy交联于卵清蛋白作为包被抗原，LBc-BSA和LBs为免疫原制备了联苯菊酯的兔抗血清，非竞争间接酶联免疫吸附分析方法测得其效价达5.12×10~4和3.2×10~5。

Two haptens of mono-p-nitrophenyl phosphonates were designed and synthesized according to the structural features of the substrate and its hydrolytic transition state. The phosphonate haptens were coupled to the carrier proteins bovine serum albumin and keyhole limpet hemocyanin .

以对硝基乙酸苯酯水解反应中间体的结构信息进行分子设计，经过Arbzov等五步化学反应合成了二种对硝基苯基类膦酸酯半抗原，将其与载体蛋白（牛血清白蛋白BSA和钥孔〓血兰素KLH）偶联，制得了具不同抗原价（10～60）的半人工合成全抗原。

Two types of haptens, the earboxylated propyl amino derivative of the acid moiety (HCA1) that is the hydrolyzed product of cyhalothrin, and the carboxylated ethyl carbonyl derivative of the alcohol moiety (HCB2) were synthesized, and conjugated with the carrier proteins bovine serum albumin and ovalbumin respectively by the active ester method and the mixed anhydride method. The polyclonal antibodies were obtained after immuning to the New Zealand rabbits.

通过对氯氟氰菊酯分子结构中的酸部分接4-氨基丁酸(HCA1)和醇部分接丁二酸酐(HCB2)，合成两种不同的半抗原，并通过碳二亚胺法将HCA1和HCB2分别与牛血清蛋白偶联制备了人工抗原HCA1-BSA和HCB2-BSA，通过活泼酯法将HCA1、HCAS和HCB2分别与卵清蛋白偶联制备包被抗原，两种人工抗原通过免疫新西兰大白兔来制备相应的多克隆抗体。

With this aim, two different haptens mono- O -3-carboxypropyl- diethylstilbestrol and mono- O -carboxymethyldiethylstilbestrol with carboxylic group that preserve the molecular structure character of diethylstilbestrol were synthesized. The haptens were conjugated with the carrier proteins bovine serum albumin by mixed-anhydride method for immunogen and conjugated with ovalbumin by active ester method for coating antigen.

首先，两种保留己烯雌酚特征结构并具有活性基团的半抗原正丁酸己烯雌酚单醚、乙酸己烯雌酚单醚被合成；然后采用混合酸酐法将半抗原与BSA偶联制备成免疫原，采用活性酯法将办抗原与OVA偶联制备了包被原。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。