查询词典 bovine

METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

采用全骨髓法体外分离培养人骨髓间充质干细胞，传至第3代以1×108 L-1密度接种至预置盖玻片的6孔培养板中，设立3组：成骨诱导组添加原代培养液后，再加入含10-8 mol/L地塞米松、10 mmol/L β-磷酸甘油、50 mg/L维生素C的成骨诱导培养基；成骨+瘦素诱导组添加成骨诱导培养基后，再加入50 nmol/L瘦素；空白对照组仅加入含体积分数为10%胎牛血清的LG-DMEM培养液。

After culture on coverslips and bovine cortical bone slices for 1, 3, 5, and 7 days, morphologic features, TRAP staining and bone absorptive lacunae were observed to evaluate osteoclast cells.

结果显示，当培养细胞在RANKL和M-CSF联合作用下，TRAP阳性染色的单核和多核破骨细胞可在3天内迅速形成，骨片吸收实验显示骨片上出现典型的骨吸收征象；在M-CSF(50ng/ml)协同作用下，RANKL呈剂量依赖型诱导TRAP阳性破骨细胞生成。

Methods: A 6-mm unilateral femoral segmental defect was surgically created in 120 Sprague-Dawley rats, internally stabilized with a polyacetyl plate and 6 Kirschner wires, and contaminated with 104 colony-forming units of Staphylococcus aureus. After 2 weeks, all defects were surgically debrided and implanted with 0, 20, or 200 g of rhBMP-2 in a type 1 bovine collagen sponge.

通过外科手术在120只Sprague-Dawley大鼠一侧股骨制造了6毫米的缺损，内部通过聚乙烯板及克氏针固定，并被104个金黄色葡萄球菌菌落污染。2周后在所有的缺损处通过1型牛胶原海绵注入0、20或200g重组人骨生成蛋白。

Methods Fresh adult bovine tendon was dissolved with acetic acid and enzyme-digestion for type Ⅰ collagen. Then they were dialysed and purified for refined liquid collagen, which was cross-linked with glutaraldehyde.

取新鲜成年牛跟腱，用乙酸溶解－酶消化法提取Ⅰ型胶原，并进行盐析、透析、纯化得到精制的液态胶原，用戊二醛进行交联。

In this study,bovine serum albumin was used as components of blocking buffer,diluents for serum and enzyme-labeled secondary antibody,respectively.

牛结核病是一种人兽共患传染病，不仅严重影响养牛业的健康发展，而且由于人类与奶牛间的密切关系，也同时严重威胁着人类的健康。

The rising sun, the back depends on phoenix hill, river of headrest old rise high, the Gong Shanwen of bovine river bridge that churchyard disentombs changes relics, for China civilization history provided another evidence.

朝阳，背依凤凰山，头枕大凌河，境内发掘的牛河梁红山文化遗址，为中华文明史提供了又一佐证。

Results In cultured bovine endothelial cells, the eNOS protein under the 5 dynes/cm2 shear stress was 1ower than that in control static endothelial cells.

结果 静息状态下，体外培养的牛胸主动脉内皮细胞呈多角形&铺路石&状排列，形态大小均匀。

An ELISA(enzyme-linked immunosorbent assay.) was developed for detection of bovine brucellosis by using LPS as antigen purified by Heating - phenol recommended by OIE (office international des epizootics).

采用世界动物卫生组织推荐的热酚法提取的脂多糖作为抗原，建立了牛布氏杆菌病的ELISA方法，对45份血清进行检测，证明本ELISA方法灵敏性较高，经阻断试验和交叉试验，证明特异性较好。

Methods Cells were maintained in DMEM medium with 10% fetal bovine serum. Five days before the beginning of experiments, the cells were seeded in phenol red-free DMEM medium containing 5% charcoal dextran-treated FBS. The cells were harvested, and seeded in 6-well culture plates or in 75ml flacks. After NP, BisA and DBP treatments for 72h, the cells were harvested, and mRNA and protein expression of PCNA, bcl-2 and bax were detected by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively.

方法］T47D细胞在含10%胎牛血清的DMEM培养液中进行常规传代培养，实验前将细胞转移至无酚红DMEM（含5%活性碳葡聚糖苷处理过的FBS）中继续培养5d，收集细胞，PBS洗涤后接种於内置盖玻片的6孔板或75ml培养瓶中，用32×10^(-7)mol/L NP、32×10^(-7)mol/L BisA及32×10^(-6)mol/L DBP对细胞分别处理72h，用半定量RT-PCR技术观察这3种化合物对核增殖抗原PCNA、bcl-2及bax mRNA表达的影响，并用免疫组化方法对结果进行验证。

Two fragments G1 and G2 of the glycoprotein G gene of bovine respiratory syncytial virus were selected for expression in Escherichia coli based on the analysis of glycoprotein G by DNA Star software.

经生物学软件DNA Star分析，将牛呼吸道合胞体病毒G基因截短成2个片段G1和G2。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。