查询词典 bovine

METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

无菌状态下取出胎儿肝脏，以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液，分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心，吸取界层细胞，添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。

METHODS: NSCs were isolated from neonatal rats by enzyme digestion and mechanical separation. At the fourth passage, cell clone masses received nestin immunocytochemistry. Remaining cells were dispersed by mechanical separation. Monoclone NSCs were incubated by limiting dilution assay, and made into 108 L-1 monoplast suspension in complete medium. NSCs were assigned into 2 groups. NSCs in the control group were incubated in 10% fetal bovine serum.

酶消化和机械分离法相结合体外分离培养新生鼠神经干细胞，传至第4代的细胞克隆团行巢蛋白免疫细胞化学染色观察，剩余细胞团用机械法分散，采用有限稀释法进行单克隆神经干细胞培养，加入完全培养基制成108 L-1的单细胞悬液，分为2组滴入培养板，对照组加入10%FBS，诱导组加入10%FBS+50 μg/L神经生长因子，培养5~7 d。

METHODS: Olfactory bulb was sterilely collected from rats, digested, and centrifuged. After removal of the supernatant, the olfactory bulb was resuspended with DMEM/F-12 medium containing 0.2 volume fraction of fetal bovine serum. The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence + serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁+无血清条件培养液纯化的实验。

Methods Marrow cells from NH mouse were harvested and cultured in α-MEM with 10% fetal bovine serum.The appearance of tartrate-resistant acid phosphatase-staining multinuclear cells and formation of bone resorption pits were measured after 3,6,9 and 12 day exposure to various concentrations of 1,25-2D3 in culture. Results 1,25-2D3 (concentration higher than 10-9mol/L) could induce recruitment of OLCs and their bone resorption activity in a dose-dependent manner.

收集NH小鼠骨髓细胞于含10%胎牛血清的α-MEM培养基中行体外培养，设置不同的1，25-2D3浓度组和给药时间组，并于培养第3、6、9、12天观察记录抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase，TRAP)阳性多核巨细胞［或破骨细胞样细胞(osteroblast-like cell，OLC)］以及骨磨片上骨吸收陷窝的数目。

Many hES cell lines have been established under different conditions in the world. The firstly established hES cell lines were cultured on mouse embryonic fibroblast cells with medium containing many undefined animal components such as fetal bovine serum, which may cause cross-transfection with animal pathogen and mycoplasm.

目前，世界上已经建立了多株hES 细胞系，最早建立的hES 细胞系是生长在小鼠胚胎成纤维(mouse embryonic fibroblast， MEF)细胞上的，培养体系中含血清等动物源性成分，这些成分可能引起动物源性病原体或支原体的污染，从而限制了 hES 细胞的临床应用。

Like other alpha herpesvirus such as herpes simplex virus type I, varicella-zoster virus and bovine herpesvirus type I, PRV also has some typical characteristics of alpha herpesvirus, including latency and neurotropism.

与同属α—疱疹病毒亚科的人单纯疱疹病毒、水痘-带状疱疹病毒和牛疱疹病毒Ⅰ型等一样，PRV具有潜伏感染和神经嗜性等α—疱疹病毒的典型特征。

Methods Mononuclear cells of peripheral blood were isolated by Ficoll Hypaque centrifugation from 32 breast cancer patients and plated on 6-well culture plates(106 /ml, 2 ml/well) in RPMI1640 medium supplemented with 10％ heat-inactivated fetal bovine serum, GM-CSF, IL-4, and/or TNF-α. Two h later, nonadherent cells were gently removed and fresh medium was added.

方法采用密度梯度离心的方法，分离32例乳腺癌患者外周血中的单个核细胞，在6孔培养板上(106/ml，2ml/孔)，用含10％热灭火的小牛血清和GM-CSF、IL-4、TNF-α细胞因子的RPMI 1640培养基培养。

Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法：穿刺抽取15 mL脐带血，密度梯度法分离吸取分层界面的白色环形絮状物，其中富含单个核细胞，离心后沉积细胞用含15％胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮，调整细胞密度为1×109 L-1接种于25 T培养瓶内，放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养，72 h后更换培养基，去除红细胞及其他未贴壁细胞，至70％~80％融合时传代。

Methods: Rat MSCs were isolated with percoll and nonadherent cells were discarded 48 hours later. The shape was observed and CD34, CD44, CD45, CD166 were detected with Flow cytometry analysis. The growth curve of MSCs in different concentration of fetal bovine serum was observed by MTT colorimetry. The growth curve and attachment efficiency of MSCs of passage 1 (P1), P3, P5 and P10 were also observed.

采用密度梯度离心法结合贴壁分离法分离培养大鼠骨髓MSCs，观察其形态，流式细胞仪检测细胞表面标记，MTT法观察不同浓度的胎牛血清对MSCs生长曲线的影响及不同代MSCs的生长曲线和细胞贴壁率。

METHODS:Antisense "knock-down" approach was used to inhibit MDR1 gene expression in bovine nonpigmented ciliary epithelial cells.

用反义寡核苷酸阻抑牛眼睫状体非色素上皮细胞MDR1基因表达，在激光共聚焦显微镜下检测MDR1基因反

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐