查询词典 bovine vaccine

The research results and expression product could serve as a basis for further studies on the gene-engineering vaccine, subunit vaccine and nucleonicacid vaccine against Bovine Paratuberculosis.

为进一步研究副结核分枝杆菌基因工程亚单位疫苗、核酸疫苗及诊断试剂奠定了基础。

Biological Qualcomm reported that since the world recognized the success of the most promising AIDS vaccine trial has been declared a failure, the date of successful AIDS vaccine seems to have become the foreseeable future, the Nobel Medicine Prize, the world's leading AIDS expert David Baldi Professor Moreton speech, claiming that the complexity of HIV based on the variability in the 25 years of human AIDS vaccine can not be found,"and most likely never find such a vaccine."

生物通报道，自从被世界公认最有希望获得成功的艾滋病疫苗试验宣布失败以来，艾滋病疫苗成功之日似乎变得遥遥无期，诺贝尔医学奖得主、世界著名艾滋病研究专家大卫·巴尔迪摩教授发表演说，声称基于艾滋病病毒复杂的变异性，人类在25 年内不可能找到艾滋病疫苗，"并且很有可能永远也找不到这种疫苗。"

The two valence vaccine consisting of catalase, adhesion conservation and adjuvant has better immunotherapeutic effects than the single valence vaccine. More H. pylori vaccine research should be directed to the multivalence vaccine.权威期刊K-Rf l4tw

由过氧化氢酶和黏附素保守区加免疫佐剂组成的二价口服疫苗较单价口服疫苗有更好的免疫治疗效果，多价疫苗是未来H.pylori疫苗的研制方向。

It is shown that G gene has the highest speed during the evolution and has uniqueinquilinous body in order to escape immune pressure from inquilinous body byanalyzing phylogenic trees of five genes in the whole genome. In the evolution of Ggene, DRV and MRV are in different groups. DRV, Chinese vaccine isolated used inhuman and Japanese vaccine isolated used in human are in the same group. MRV,CVS and street viruses isolated from dogs are in the some group. It is shown thatDRV is vaccine isolated compared with phylogenic trees of other genes, and it istestified previous conclusion that unqualified vaccine used for human was used nearthe infected beer cote resulted in spotted deer infected.

对狂犬病毒基因组5个基因的分子进化树分析表明，为了逃避宿主的免疫压力，G基因的进化最快速，而且具有宿主的特异性，在G基因的进化中，DRV和MRV分别位于不同的组群，DRV与中国人用疫苗株和日本疫苗株在一个组群，MRV与CVS以及犬脑分离的街毒归在一个组群，结合其他基因的进化树表明，DRV与疫苗株具有较近的亲缘关系，从分子水平验证了以前对DRV流行病学调查得出的推测，在狂犬病疫情发生的鹿场附近曾使用过人用狂犬病疫苗，导致梅花鹿感染狂犬病毒。

DTP acellular vaccine shortage stage, in accordance with section four times to the order of a meeting with a cell-free alternative to DPT vaccine DPT vaccine; less than part of the continued use of DPT vaccine.

无细胞百白破疫苗供应不足阶段，按照第4剂次至第1剂次的顺序，用无细胞百白破疫苗替代百白破疫苗；不足部分继续使用百白破疫苗。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

Above all, Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli are the main pathogens causing bovine subclinical mastitis; the bovine clinical mastitis is usually caused by Streptococcus agalactiae. Antibiotics such as Enrofloxacin, Erythromycin and Kita sermonic show effective on treatment; the preparation of Chinese traditional medicine has great potential on bovine mastitis treatment and should be widely spread.

实验结果说明：引起奶牛隐性型乳房炎的主要致病菌类型为无乳链球菌、金黄色葡萄球菌和大肠杆菌，引起临床型乳房炎的主要致病菌是无乳链球菌；抗生素中的恩诺沙星、红霉素、庆大霉素用来治疗奶牛乳房炎效果较理想，中药制剂在治疗乳房炎上应用潜力很大，应大力推广。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

ResultsRecombinant bovine prochymosin was not significant difference in molecular weight,immune characteristics, bioactivity and sensitivity of inhibitors comparison to nature bovine prochymosin.The milk-clotting activity of recombinant bovine prochymosin was 2×103 international milk-clotting unit per millilitre.

重组牛凝乳酶原与天然牛凝乳酶原比较，其分子量大小、免疫性质、生物活性和抑制剂敏感性没有发现显著差异，其凝乳活性可达2×103 IMCU/mL。

Forth, cows were immunized infected bovine rotavirus vaccine, and then the effect of passive antibody from colostrums on immunity to bovine rotavirus infections in neonatal calves was investigated.

最后，将研制的牛轮状病毒灭活疫苗免疫孕牛后，研究产下犊牛通过母源抗体获得的被动免疫在预防牛轮状病毒感染中的作用。

Article 144 The Government of the Hong Kong Special Administrative Region shall maintain the policy previously practised in Hong Kong in respect of subventions for non-governmental organizations in fields such as education, medicine and health, culture, art, recreation, sports, social welfare and social

第一百四十四条香港特别行政区政府保持原在香港实行的对教育、医疗卫生、文化、艺术、康乐、体育、社会福利、社会工作等方面的民间团体机构的资助政策。原在香港各资助机构任职的人员均可根据原有制度继续受聘。

Small wonder, then, that the Chinese spend more in the shop than any other group of foreign visitors do .

这样的小惊喜，使中国顾客比任何国家的人消费得更多。