查询词典 bovine pest

The cells were cultured under the following serum microenvironment. The primary cells of autoserum group were cultured with autoserum, changing the medium with fetal bovine serum after passage. The primary cells of homogeneity foreign serum group were cultured with homogeneity foreign serum, changing the medium with fetal bovine serum after passage. The primary cells of fetal bovine serum group were cultured with fetal bovine serum, and cultured with fetal bovine serum after passage. The primary cells of Dulbecco's modified Eagle's medium group were cultured with serum-free DMEM, changing the medium with fetal bovine serum after passage.

分别以下列血清微环境培养：自身血清组：分离细胞后原代用含大鼠自身血清的培养基培养，传代后换用含胎牛血清的培养基培养；同种异体血清组：分离细胞后原代用含同种异体血清的培养基培养，以后用胎牛血清培养基培养；胎牛血清组：分离后用含胎牛血清培养基培养，以后一直用含胎牛血清培养基培养；DMEM组：分离细胞后原代用不含血清的DMEM培养基培养，传代后换用含胎牛血清的培养基培养。

Therefore, we at the Marriott Hotel, focused on pest control in the following aspects: one, a sound pest control project reporting system, including records of drug use, pest density and the improvement of means of pest against recommendation 2, and further improve the pest control facilities, in particular, Hotels are the physical control such as fly disinfestation lamp.

所以，我们在万豪大酒店的虫害控制中着重以下几方面：1、完善的虫害控制工程报告制度，内容包括药物使用记录、虫害密度及虫害侵害途径的改善建议2、进一步完善防虫设施，尤其是酒店的物理防治{如灭蝇灯调整及管理}。

This thesis introduces the domestic and international research development on techniques of preventing against and killing archives pests briefly at first, on the basis of advantages and disadvantages of chemical 、 physical、 biological pest-killing techniques and practice of realistic archives preservation,determines to research lethiferous effectiveness of high temperature pest-killing technique、 low temperature pest-killing technique、 microwave pest-killing technique and high pressure nitrogen suffocation pest-killing technique,and affect on durability of making materials of paper archives systemically.

本论文首先简要介绍了国内外档案害虫防治技术研究进展，在分析化学防治法、物理防治法和生物防治法的优缺点及现实档案保管工作实践的基础上，确立系统研究高温杀虫法、低温杀虫法、微波杀虫法、高压充氮杀虫法的杀虫效力及对纸质档案制成材料耐久性的影响。

This thesis analysis lethiferous effectiveness of high temperature pest-killing technique、 low temperature pest-killing technique、 microwave pest-killing technique and high pressure nitrogen suffocation pest-killing technique, and affect on tensile strength、 wearproof strength、 whiteness of copypaper、 writing paper、 rice paper and newsprint, affect on colour difference of red 、 blue 、 blue-black ink colored writing, and black ink 、 laser printing 、 xerox,、 gushing typing black writing caused by four physical pest-killing techniques above-mentioned.

本论文系统分析了高温杀虫法、低温杀虫法、微波杀虫法和高压充氮杀虫法的杀虫效力，并在档案保护技术学领域较系统地探讨了四种物理法杀虫处理对复印纸、书写纸、宣纸、新闻纸的抗张强度、耐破度、白度的影响，以及对红、蓝、蓝黑墨水彩色字迹，黑墨水、激光打印、静电复印、喷墨打印黑色字迹色差的影响。

Along with our country having entered WTO and the world economic quickly inosculating as a whole,our country will faces more and more pressure of the alien pest invading.According to the related international standards and protocols, joining together the invading state of alien pest in our country,we should establish the related alien pest risk method standards and construct an effective risk management measure system to keep the alien pest from invading, it has the important meaning to protect the local agriculture,forestry ,fishing,and environment from destroying,also it has the important meaning to build the stable society.

随着我国加入WTO和全球经济一体化进程的加快，我国面临外来有害生物入侵的风险也越来越大，如何根据相关国际标准和协议，结合我国的国情，研究相关的外来有害生物风险分析方法，建立良好的风险管理措施体系，防止外来有害生物的入侵，保护国内农林渔业生产的安全、生态环境的安全、维护社会稳定构筑和谐社会具有重要的意义。

Electroantennogram responses to several chemical volatiles of soybean plant were investigated for the following four species of insects: 1 Aphis craccivora Koch and Macrosiphum avenae; 2 Lysiphlebus fabarum Marshall, the common nature enemy of Aphis glycines Matsumura which is the pest insect of soybean plant, and of A.craccivora which is not the pest insect of soybean plant; 3 Aphidius picipes Nees, the nature enemy of M.avenae which is not the pest insect of soybean plant.

研究了下列害虫和寄生天敌种类对大豆植株中提取的某些挥发性次生化合物及其不同组合混合相的触角电位反应： 1豆蚜Aphis craccivora Koch和麦长管蚜Macrosiphum avenae；2为害大豆植株的大豆蚜Aphis glycines Matsumura和不为害大豆植株的豆蚜二者所共有的寄生天敌豆柄瘤蚜茧蜂Lysiphlebus fabarum Marshall； 3不为害大豆植株的麦长管蚜的寄生天敌燕麦蚜茧蜂Aphidius picipes Nees。

Result 10 308 insects were identified, belonging to 9 orders, 43 families, 68 species of Insecta and I families, 1 species of Arachnida. The result revealed that the individuals of the pest sub-community were more than that of the natural enemies sub-community, the dominant concentration and dominance of the pest sub-community were the biggest, diversity index of the natural enemies sub-community was more than that of the pest sub-community.

结果］共调查昆虫10 308头，分属于昆虫纲9个目、43科6、8种以及蛛形纲1科、1种；野生植物害虫亚群落的物种个体数多于天敌亚群落的个体数，害虫亚群落的优势集中性和优势度最大，天敌亚群落的多样性指数大于害虫亚群落。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

Above all, Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli are the main pathogens causing bovine subclinical mastitis; the bovine clinical mastitis is usually caused by Streptococcus agalactiae. Antibiotics such as Enrofloxacin, Erythromycin and Kita sermonic show effective on treatment; the preparation of Chinese traditional medicine has great potential on bovine mastitis treatment and should be widely spread.

实验结果说明：引起奶牛隐性型乳房炎的主要致病菌类型为无乳链球菌、金黄色葡萄球菌和大肠杆菌，引起临床型乳房炎的主要致病菌是无乳链球菌；抗生素中的恩诺沙星、红霉素、庆大霉素用来治疗奶牛乳房炎效果较理想，中药制剂在治疗乳房炎上应用潜力很大，应大力推广。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

In the days of sailing vessels the crew were afraid they would be becalmed on the ocean.

在使用帆船的时代，船员们担心他们会因为无风而无法在海洋上航行。

As long as foreign donors pay the PA's salary bill, few expect a new intifada.

只要外国继续为巴权力机构的薪水买单，希望发动新暴动的人便寥寥无几。