查询词典 bovine lymph

The cells were cultured under the following serum microenvironment. The primary cells of autoserum group were cultured with autoserum, changing the medium with fetal bovine serum after passage. The primary cells of homogeneity foreign serum group were cultured with homogeneity foreign serum, changing the medium with fetal bovine serum after passage. The primary cells of fetal bovine serum group were cultured with fetal bovine serum, and cultured with fetal bovine serum after passage. The primary cells of Dulbecco's modified Eagle's medium group were cultured with serum-free DMEM, changing the medium with fetal bovine serum after passage.

分别以下列血清微环境培养：自身血清组：分离细胞后原代用含大鼠自身血清的培养基培养，传代后换用含胎牛血清的培养基培养；同种异体血清组：分离细胞后原代用含同种异体血清的培养基培养，以后用胎牛血清培养基培养；胎牛血清组：分离后用含胎牛血清培养基培养，以后一直用含胎牛血清培养基培养；DMEM组：分离细胞后原代用不含血清的DMEM培养基培养，传代后换用含胎牛血清的培养基培养。

Results A total of 555 lymph nodes were harvested, with 99 lymph nodes in 27 casas found positive. 72 lymph nodes in 267 lymph nodes in the paratumoral were found positive. 21 lymph nodes in 165 lymph nodes in the proximal tumoral were found positive.

评估肠癌患者分期，淋巴结浸润是最主要的预后因子[2]，肿瘤转移检出率随淋巴结发现数的增高而升高，免疫组化技术的应用提高了阳性淋巴结的检出率。

The average number of anterior lymph nodes was 3.83±1.79. Their blood supply originated from the branches of lateral thoracic artery, whose mean diameter and length were 1.04±0.42 and 29.5±12.25 respectively.(3) The average number of posterior lymph node was 2.38±0.97. Their blood supply originated from the lymph node branch of subscapular artery, whose length and mean diameter were respectively 18.78±8.67 and 0.78±0.32.(4) The average number of central lymph nodes was 4.05±1.95. Their blood supply originated from axillary artery whose mean diameter was less than 1mm.(5) The average number of apical lymph nodes was 2.82±1.96. Their blood supply originated from the branches of thoracoacromial artery whose mean diameter was 0.79±0.29mm and length was 10.60±4.13mm.

结果 外侧群淋巴结数量为(3.16±1.72)个，血供属多源性且不恒定；前群淋巴结数量为(3.83±1.79)个，营养血管主要为胸外侧动脉的分支，其外径(1.04±0.42)mm，干长(29.5±12.25)mm；后群淋巴结数量为(2.38±0.97)个，血管来源于肩胛下动脉的淋巴结支，其外径为(0.78±0.32)mm，干长(18.78±8.67)mm；中央群淋巴结数量为(4.05±1.95)个，血管来源于腋动脉的分支，其外径均在1mm以下，干长20mm以上；尖群淋巴结数量为(2.82±1.96)个，营养血管为胸肩峰动脉的分支，外径为(0.79±0.29)mm，干长(10.60±4.13)mm。

Results:By chlorophyll disclosing solution coloring lymph nodes, we found, by injecting the solution into rectal wall of mouse, it colored lymph nodes quickly(in 30 minutes) and was not easy to precipitate. The dyeing lymph nodes showed emerald green, it contrasted sharply with surrounding tissues and no color fading after injected 24 hours. The average number of lymph nodes observed in each mouse was 6.92, which was more than that of LD1 group significantly(P.01). No adverse reaction or complication were observed in the course of injection and after injection, And the ultrastructure of the lymph nodes colored at different interval was no significant difference to the ones uncolored.

结果：于直肠壁内注射CDS，淋巴结染色迅速，不易沉淀，各级哨兵淋巴结在30 min内被染成翠绿色，与周围组织对比明显；LD2组平均每鼠检出淋巴结6.92个，显著高于LD1组（P.01）；染色淋巴结24 h后仍无褪色，且染色后细胞超微结构无明显改变；术中和术后均未发现不良反应和并发症。

Methods After routine Whipple procedure,the standardized regional clearance of mesenteric root lymph node,lymph node beside the abdominal aorta,intrahepatic and intra-duodenal ligamentous lymph node,parahepatic arterial lymph node and paraceliac arterial lymph node was performed. Results A total of 11 patients were enrolled in this study.

在常规Whipple基础上，进行规范化区域淋巴结清扫，重点清除肠系膜根部淋巴结(14 abcd)、腹主动脉旁淋巴结(16 a2b1)、以及肝十二指肠韧带内淋巴结(12 abpch)、肝动脉旁(8组)、腹腔动脉旁(9组)淋巴结。

Traumatic or surgical injuries to this system can cause leakage of lymph and result in the formation of a lymphocele, which is defined as an accumulation of lymph surrounded by pseudomembranes (layers of tissue with a reaction of the surrounding tissue).10 Lymph drainage above the cisterna chyli is rich in chylomicrons and has a creamy white appearance because of the contribution of the gastrointestinal lymphatics.14 Below the cisterna chyli, however, lymph is serous in nature and has a clear yellow color and a significantly lower triglyceride content.

外伤或手术对这个系统造成损伤可以引起淋巴漏，最终导致淋巴管瘤形成。淋巴管瘤即为有假膜（由于四周组织反应形成的组织层）包裹的淋巴聚集。由于胃肠道淋巴的关系，在乳糜池以上的淋巴管富含乳糜微粒，有奶油样的白色外观。而乳糜池一下的淋巴则为浆液性质，为清亮黄色，其甘油三脂的含量明显降低。

Tuberculous lesions were identified in 27 cattle (47.4%; n = 57) with the evidence on gross and/or microscopic examination in 1 or more sites. The most frequently affected site was retropharyngeal lymph node, followed by mediastinal lymph node, hilar lymph node, and mesenteric lymph node.

结果显示，所收集的结核菌素检测阳性牛中，有 27 头（47.4%； n = 57）在肉眼或／及显微检查下发现有结核样病灶，其中最常出现病变的位置为咽背淋巴结，其次依序为纵膈淋巴结、肺门淋巴结和肠系膜淋巴结。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

Above all, Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli are the main pathogens causing bovine subclinical mastitis; the bovine clinical mastitis is usually caused by Streptococcus agalactiae. Antibiotics such as Enrofloxacin, Erythromycin and Kita sermonic show effective on treatment; the preparation of Chinese traditional medicine has great potential on bovine mastitis treatment and should be widely spread.

实验结果说明：引起奶牛隐性型乳房炎的主要致病菌类型为无乳链球菌、金黄色葡萄球菌和大肠杆菌，引起临床型乳房炎的主要致病菌是无乳链球菌；抗生素中的恩诺沙星、红霉素、庆大霉素用来治疗奶牛乳房炎效果较理想，中药制剂在治疗乳房炎上应用潜力很大，应大力推广。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

Liapunov—Schmidt method is one of the most important method in the bifurcation theory.

Liapunov—Schmidt方法是分叉理论的最重要方法之一。

Be courteous -- even when people are most discourteous to you .

要有礼貌──即使当別人对你最不礼貌的时候。