查询词典 bound to

To prepare antibodies against MG, Pararosaniline was bound to BSA or OVA using nitrous acid as the coupling reagent, and using UV spectrophotometer scanning identified the conjugates.

为了制备针对MG及其代谢产物的抗体，将MG代谢相似物之一副品红用重氮化法偶联到牛血清白蛋白，获得免疫原BSA-PA。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

He therefore criticized Leibniz, saying the main disadvantage of pictography – in comparison to alphabetical writing – was that the essence of the former is bound to the "sensuous-concrete", while only the latter manages to capture the abstract and the formal.

因此他批评莱布尼兹，说比起拼音书写，象形文字的短处是它被'感官-实形'所困，只有拼音文字才能捕捉抽象和形式。

It's time to pierce the veils of illusion which keep us bound to an unreal world.

现在是时候皮尔斯的面纱幻想保持我们一定会一个虚幻的世界。

One of them,Pirithous,was bound to a turning wheel while Theseus himself was chained to a rock.

他们出发了，但他们却发现自己已无法逃脱，皮瑞塞斯被绑到了一个旋转的车轮上了，而特修斯则被拴在了石头上。

Even the best system is bound to have some holes, but good records will allow you to find them and plug them up.

再好的交易系统都有漏洞，但是好的交易记录能让你找到它们并修补漏洞。

When the pointer is dereferenced, it must be bound to an object of or pointer to type C

对该指针解引用的时候，它必须是绑定到类型 C 的对象或指向类型 C 的指针

And there are plenty of opportunities to engage in retail therapy; while Harrods or Selfridges might be beyond your price range, you're bound to find a bargain in Camden Market or down the Portobello Road.

伦敦有着无限的商机，在这里，人们可以从事各种商业活动。如果你觉得哈罗德百货公司、塞尔弗里奇公司百货商店的价格有些高，那么也可以去卡姆登市场或者贝罗大街的地摊上讨价还价。

Mourinho then moved quickly to pre-empt speculation that was bound to follow the new addition to the squad.

穆里尼奥随后很快转入占有先机的带来这个阵容中的新援的转会。

The said electors, both cardinals and others, are also bound to swear, before they proceed to the election, that in attending to the business of the election, they will proceed with pure and sincere minds--since it is a question of creating the vicar Jesus Christ, the successor of the blessed Peter, the governor of the universal church and the leader of the Lord's flock--and that they firmly believe it will benefit the public good of the universal church if they entirely prescind from all affection for persons of any particular nation, or other inordinate affections, as well as from hatred and graces or favours bestowed, in order that by their ministry a beneficial and suitable pastor may be provided for the universal church.

该说的选民，无论是红衣主教等，也必将有宣誓，然后进行选举，即在抓业务的选举，他们将着手纯洁和真诚的心-因为这是一个问题的创造副主教耶稣基督，继任人的祝福，彼得总督的普世教会和领导上帝的羊群-而且他们坚信，这将有利于公益事业的普世教会，如果他们完全p rescind从所有感情的人任何特定的国家，或其他念情，以及从仇恨和青睐恩惠赐予，为了使他们的财政部是一次有益的和合适的牧师可以为人民群众提供了普世教会。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。