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Molecular tree showed that haplotypes from Bubalus bubalis and Bos indicus clustered on their own and there were apparent differentiation between Bubalus bubalis, Bos indicus and other bovine species. There was no apparent differentiation among Bos taurus, Bos grunniens, Bos frontalis and they maybe produced apparent differentiation gradually in the process of evolution.

分子进化树显示,水牛、瘤牛独自成类并与其他牛种显著分化;普通牛、牦牛、大额牛间的序列分化程度并不明显,但有可能在进化过程中逐步产生明显的分化。

Mutation rate at exon of GH in five bovine species was very low with the percentage of 3.48%. The majority of nucleotide substitution was nonsense mutation and only one missense mutation was observed. Molecule tree based on haplotypes of bGH at exon 5 showed that differentiation was apparent relatively between Bubalus bubalis and Bos taurus, Bos indicus, Bos grunniens, Bos frontalis. There was no apparent differentiation among other four bovine species and they shared mutual ancestral sequence.

序列分析表明,5个牛种GH基因外显子5的遗传变异水平较低,平均核苷酸变异率约为3.48%,而且绝大多数位点的核苷酸替换是同义突变,仅发现1个错义突变位点;从GH基因外显子5序列单倍型构建的分子进化树来看,水牛与普通牛、瘤牛、牦牛及大额牛的分化相对比较明显,其他4个牛种之间并无明显分化,还享有共同的祖先序列。

Myostatin gene sequence of Notheast Wapiti was cloned based on the closest species of cattle (Bos taurus cattle accession: AB076403) for the templates, with primer's design according to the conservative region of MSTN gene.

以物种最近的牛(Bos taurus cattle登陆号:AB076403)的Myostatin基因序列为模版,根据该基因保守序列,进行引物设计。

Methods: water decocting method for extracting method, ether as extraction solvent with high performance liquid chromatography, as the means, to test content of methanol 0-3 % diethylamine (73:27) as mobile phase, 242nm detected wavelength, according to the packing: Hypersil BOS C18 5 muon m size: 6 mm (4): 25 mm, standard curve basic linear, precision, accuracy, good stability and.

以水煎煮法作为提取方法,乙醚作为萃取溶剂,以高效液相色谱作为含量检测的手段,以甲醇-0。 3 %二乙胺(73∶27)作为流动相,检测波长242nm,依利特填料:Hypersil BOS C18 5μm 尺寸:4。 6 mm ×250 mm ,结果:标准曲线基本呈线性,精密度,稳定性和准确度良好。

Extensive research efforts with special emphasis on innate immunity have recently led to new insights with the identification of at least two different phenotypes: on the one hand there is an azithromycin-responsive phenotype the so-called neutrophilic reversible allograft/airways dysfunction (NRAD, on the other hand there is an azithromycin-unresponsive phenotype (the fibroproliferative form of BOS or classical obliterative bronchiolitis).

近来,重点关注天然免疫的大量研究得出了新视角,鉴定了至少2种不同的表型:第一种是对阿奇霉素有反应的表型(所谓的中性粒细胞可逆性移植/起到功能障碍),另一种是对阿奇霉素无反应的表型(BOS的纤维增生型或经典的闭塞性细支气管炎)。

After restimulation in vitro with HTHV plus rhuIL-2, the splenocytes either from HTHV infected mice or from pEF-BOS/HTHV-NP plasmid DNA immunized mice were used as effectors in 4 h 51 Cr release assay.

分别取HTHV感染小鼠的脾细胞和pEF-BOS/HTHV-NP质粒DNA免疫小鼠的脾细胞,经体外抗原和IL-2再刺激诱导产生CTL效应细胞,进行4h 51 Cr释放实验。

KingdeeK/3 BOS is a new model of Kingdee K/3 series which is object to basic operation designing.

金蝶K/3 BOS——是金蝶K/3商业操作系统,是针对金蝶K/3系列产品研发的新一代业务基础设计平台。

KingdeeK/3 BOS is a new model of Kingdee K/3 series which is object to basic operation designing.

金蝶K/3 BOS——是金蝶K/3商业操作系统,是针对金蝶K/3系列产品研发的新1代业务基础设计平台。

BOS series auto parts, for example, sunshade cassette, cargo cover extrusion and clearstory orbit, etc.

小红旗系列轿车门内板焊接总成;2。雪佛兰轿车转向器防护总成;3。 BOS系列汽车内饰件;系列窗帘支架、天窗导轨

Involve in evaluation and implementation of BOS/HOS solution project.

参与评价与实施BOS/HOS解决方案项目。

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If you have any questions, you can contact me anytime.

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