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Methods Pregnancy mice were divided into four groups randomly. From the beginning of pregnance,the mice in experimental groups were given BPA with 30,120,360 mg·kg-1·d-1 until the new mice were born. The mice in control group were given the same volume of corn oil.

妊娠小鼠随机分成4组，从受孕第1天起实验组分别以30、120和360 mg·kg-1·d-1剂量溶于玉米油中的双酚A染毒至仔鼠出生，对照组用相同体积玉米油灌胃。

Six hundred ova were transfered into twenty-nine pseudopregnant recipients. Fifty-five offspring were born from the ova mircroinjected with the Bcl-2 fragment expression cassette.Twelve of them were proved to be gene integration positive by PCR .Four founder mice,two male and two female ,were further confirmed to be transgenic by Southern hybridization.

用BglII+SalI+PvuI三酶切pWBS和用BglII+BamHI双酶切pWCS，分别回收并纯化3.0kb的基因片段WAP-Bcl-2-SV40polyA和3.5kb的基因片段WAP-c-myc-SV40poly，作为目的基因，通过受精卵显微注射技术，分别导入C57BL／6J雌鼠与DBA／2J雄鼠交配的F1代小鼠受精卵的雄原核中，各注射约1000枚，将存活的受精卵各600枚分别移植至29只假孕母鼠输卵管壶腹部内。

However, the recent discoveries are overturning the traditional opinions. Nuclear-born tRNA can be exported out of nucleus, while cytoplasmic-residing tRNA can also be retrogradely transport into nucleus.

然而，最近的研究成果在颠覆传统观念，tRNA不但能被转运出核，还能被逆向转运入核。2008年，新概念&tRNA核质动态分布&(tRNA nuclear-cytoplasmic dynamics)被提出，取代tRNA核质运输，描述tRNA在细胞核、质间的流动。

And I want to start today by saying how grateful I am to all of you, to everyone who poured your hearts and your hopes into this campaign, who drove for miles and lined the streets waving homemade signs, who scrimped and saved to raise money, who knocked on doors and made calls, who talked, sometimes argued with your friends and neighbors…… who e-mailed and contributed online, who invested so much in our common enterprise, to the moms and dads who came to our events, who lifted their little girls and little boys on their shoulders and whispered in their ears,"See, you can be anything you want to be."… like 13-year-old Anne Riddell from Mayfield, Ohio, who had been saving for two years to go to Disney World and decided to use her savings instead to travel to Pennsylvania with her mom and volunteer there, as well.To the veterans, to the childhood friends, to New Yorkers and Arkansans…… who traveled across the country, telling anyone who would listen why you supported me. And to all of those women in their 80s and their 90s…… born before women could vote, who cast their votes for our campaign.

我想以表达对你们所有人的感谢来开始——所有倾注了你们的心血和希望的人、不远万里开车赶来街道两旁挥舞自制的标语的人、靠精打细算地节省来筹集资金的人、敲门拜访和致电的人、因谈论我而与朋友或邻居争论的人…………给我发过电子邮件和上网的人、给我们大众企业投资巨款的人、来到选举现场把女儿或儿子举在肩头并对他们轻声耳语&瞧，只要你想，你可以做成任何事&的妈妈爸爸们、年轻人们…………比如13岁的来自俄亥俄州Mayfield的Anne Riddell，她为了去迪斯尼乐园攒了两年的钱可后来还是决定用她的积蓄和妈妈一起来Pennsylvania做一名志愿者、还有老兵们、小朋友们、纽约以及阿肯色州的朋友，你们千里迢迢赶来，告诉所有愿意听的人你为什么支持我、还有八九十岁的出生在妇女不能参加选举的年代里的妇女们，她们曾在选举中投票。

Yang Cheng town is divided into the East Sea, center Village and the West Sea, today's re-yuan Temple was built in the then West SeaThe 20th century, in 1948, I was born in the center village's office of Yangcheng Lake township.

阳澄村分东海、中村和西海，现今的重元寺就是修建在当时的西海。20世纪的1948年，我出身在阳澄湖村的中村的乡公所。

Leaves can be born or dry food for plants, solanaceae ningxia medlar and Chinese wolfberry leaves, taste bitter Kennedy and gas cooler, into the lung and kidney.

叶可生或晒干食用，为茄科植物宁夏枸杞及枸杞的枝叶，性味苦甘而气凉，入肺、肾经。

In 1817 AD Schumacher Schneider ,born in tailor family ,succeeded in bringing the fine tailorship into the bedding manufacturing for the purpose of developing his family busuness .In 1862 AD Schumacher founded his own bedding brand ,D EIN RAUM,and decided to bring the best bedding in the world to all life lovers.

公元1817年，出生裁缝世家的舒马赫·史奈德为光大祖业，成功的将精美的裁缝工艺融入到寝具制造当中。1862年，舒马赫·史奈德先生创立了自己的寝具品牌&DEINRAUM&，并决心将世界上最好的寝具带给热爱生活的人们。

But it was not the first death that has been connected to Tilikum. In 1999, a trespasser who had apparently climbed into the orca's pool was found dead. In 1991, the born predator joined two other killer whales in drowning their trainer.

但是死在 Tilikum 手下的人不止一个了。1999年有人很明显是自己爬进鲸池中被咬死的。1991年这个生就得食肉动物和其它两条鲸鱼合伙把训练员淹死了。

Anti-globalization was again hanged on people's mouths with the born of Hongkong Announcement, in this forgotten corner, when we looking at commercialized canteens, bathhouses and dormitories, a thought which replaced resistance was prevailing. Meeting became shorter, greetingsstayed inpast, everybodywere thinkingabout an ultimation, although there is still a year. In internet, friends and classmates became taciturn because of hard-working, the School which you can achieve directly by 62 was locked deeply into memory. Some got happiness, some got rich, most sank and floated in the society. Once you became mediocre, it washard to get back.

香港宣言推出的同时，反全球化又一次成为大家的话题，在这个被遗忘的角落，我们看着被物化的食堂、澡堂和宿舍楼，一种替代了反抗的思绪在萦绕，见面变得短暂起来，寒暄也成为过去，所有人都在思考着终结，虽然毕竟还有一年，互联网上，朋友和同学也因奔波操劳而变得沉默寡言，那个62路直达的学校也被锁在记忆深处，有人得到幸福，有人发家致富，更多的人，在社会中挣扎沉浮。

This material is compressed into a shell and further excites by the intense ultraviolent light emmitted from the new born star, resulting in the glowing bubble seen here.

这种星云是会自己发光的，举例，就如这个星云，看到的星云是於中间年幼的恒星所发出的气体与周围的气体相撞时发光。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。