查询词典 blue-collar

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

In this study, nonionic surfactant sorbitan trioleate (Span 85) was modified with Cibacron Blue F-3GA to become an affinity surfactant (CB-Span 85) and to form affinity-based reversed micelles while dissolved in hexane. Since the CB molecule possesses a specific conformation that mimics nicotinamide adenine dinucleotide, it can bind strongly and specifically to a wide range of nucleotide-dependent enzymes such as dehydrogenases and kinases. Moreover, high protein transfer efficiency in the reverse micelles can be easily achieved by controlling the ionic strength of the aqueous phase.

本实验使用非离子型界面活性剂Span 85与蓝色染料分子Cibacron Blue F-3GA结合，形成具有亲和性的界面活性剂 CB-Span 85，由於CB染料分子具有与NAD+相似的结构，可与含核苷酸部位的蛋白质具有亲和力，将CB-Span 85溶於有机溶剂形成反微胞相，可有效的提高反微胞的选择率，可以只控制离子强度来调节蛋白质进出反微胞的效率。

The cytotoxicity of DC-stimulated T cells against primarily cultured hepatoma cells was estimated by using trypan blue exclusion test.

另外用Trypan blue来评估经过树突细胞活化后的混合淋巴细胞其对肝癌细胞的抑制能力。

So she retreated to Yale, graduating in 1987 with a degree in American studies, back in Hollywood she chose her projects sparingly; some were better received than others (notable, Devil in a blue dress and the indies Roger Dodger and The Anniversary Party).

所以她退避回到耶鲁大学，在1987年取得美国文学的文凭。之后回到好莱坞，她很谨慎选择要参与的工作。其中有些比较广为人知的（较知名的有Devil in a Blue Dress，独立制片的Rodger Doger及The Anniversary Party）。

I was embarrased, but sadly it's just the kind of shallow mediocrity that now passes as entertainment on our TVs and in the media - we even had a reality show winner, and a Blue Peter contestant - do you think if we'd had the same deal back in the 1970s we have offered up Bobby Crush?

真人秀冠军，Blue Peter（译注：BBC 1958年开播的儿童电视节目，详见 http：//en.wikipedia.org/wiki/Blue_Peter ）的参赛者——你们觉得我们一定得&享受&Bobby Crush（英国有名的钢琴家，6次表演让他成名，后在接受Gay time采访时出柜）在1970年给我们整的那些东西吗？

In this study, hemochromatosis identified by Berlin blue stain has been diagnosed in 52 out of 186 (28%) avian necropsies. The disease affects 11 avian orders.

本研究共收集186只圈饲禽类解剖例，其中有52只(28%)以Berlin blue铁染色来确认罹患血色素沉著症，其中包括11个目，最常郁积的组织以肝脏为主，严重者伴随肝细胞坏死且呈轻重不等程度的铁染色阳性。

The history of music in the villages, we have seen a number of Games after another girl singers:妮雅塔克Tan唱红"Delta Dawn", the only 13-year-old Mario Desmond to "Paper Roses" when fame is also 13 years old, and LeAnn Rimes to "Blue" for the first time caused a sensation when the same 13-year-old.

在乡村歌坛的历史上，我们曾经陆续看到过好些大放异彩的少女歌手：谭妮雅塔克唱红「Delta Dawn」的时候，才十三岁，玛莉奥斯蒙以「Paper Roses」成名的时候，也是十三岁，而黎安莱姆丝以「Blue」首度造成轰动的时候，同样也是十三岁。

Therefore, it is concluded that the coarse resolution MODIS image can be utilized in agricultural crop sown area extraction, the most appropriate MODIS temporal data for such applications are the combination of Red, Blue, NIR, ESWIR bands at tasseling stage and EVI image at emerging stages of winter wheat.

可见，利用Terra/MODIS数据提取冬小麦播种面积是完全可行的。研究还发现，最佳的MODIS数据是冬小麦抽穗期的Red、Blue、NIR、ESWIR波段图像和冬小麦播种期与抽穗期EVI差值图像的组合。

"A species of kingfisher, the blue-winged kookaburra delivers a shrieking cackle reminiscent of manic laughter; two types of kookaburras inhabit Australia."

翠鸟家族的一种，蓝翼笑翠鸟（blue-winged kookaburra）发出尖利的咯咯声，像极了狂躁的笑声，有两种类型的笑翠鸟栖息在澳大利亚。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。