查询词典 blotting

The expressed His-fusion protein was shown to be immunologically active by Western blotting.

经ELISA和Western Blotting检测，表达的重组蛋白具有免疫学活性。

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

METHODS: Fortyfive aged healthy crossbred dogs (15±7) kg were divided into 3 groups: tetramethlpyrazine group, verapamil group and control group. The operation was performed to ligate the near part of the LAD through left minimal thoracic incision to block 75% of the blood flow respectively. The expression changes of endothelium derived factors NO, ET1, sPselectin and CTnT before ligation and at different time points after ligation were detected and Pselectin gene of cardiac muscle was determined by Western blotting.

将45只犬分为川芎嗪，维拉帕米和对照组3组，结扎LAD阻断血流75%，分别测定3组结扎前及结扎后不同时段的分泌型P选择素、内皮素1（ET1）、一氧化氮及心肌肌钙蛋白T的含量变化，Western blotting法检测心肌组织Pselecntin基因表达情况。

METHODS: Fortyfive aged healthy crossbred dogs (15±7) kg were divided into 3 groups: tetramethlpyrazine group, verapamil group and control group. operation was performed to ligate the near part of the LAD through left minimal thoracic incision to block 75% of the blood flow respectively. The expression changes of endothelium derived factors NO, ET1, sPselectin and CTnT before ligation and at different time points after ligation were detected and Pselectin gene of cardiac muscle was determined by Western blotting.

将45只犬分为川芎嗪，维拉帕米和对照组3组，结扎LAD阻断血流75%，分别测定3组结扎前及结扎后不同时段的分泌型P选择素、内皮素1（ET1）、一氧化氮及心肌肌钙蛋白T的含量变化，Western blotting法检测心肌组织Pselecntin基因表达情况。

METHODS: Forty five aged healthy cross bred dogs (15±7) kg were divided into 3 groups: tetramethlpyrazine group, verapamil group and control group. The operation was performed to ligate the near part of the LAD through left minimal thoracic incision to block 75% of the blood flow respectively. The expression changes of endothelium derived factors NO, ET 1, sP selectin and CTnT before ligation and at different time points after ligation were detected and P selectin gene of cardiac muscle was determined by Western blotting.

将45只犬分为川芎嗪，维拉帕米和对照组3组，结扎LAD阻断血流75%，分别测定3组结扎前及结扎后不同时段的分泌型P 选择素、内皮素 1（ET 1）、一氧化氮及心肌肌钙蛋白T的含量变化，Western blotting法检测心肌组织P selecntin基因表达情况。

Micrococcal lysis assay of the milk samples showed that the cDNA was efficiently expressed in the mammary glands with similar expression levels ranging from 60 to 87 mg/L. Different tissues of each vector-injected mouse were assayed for transcription of hLYZ mRNA by dot-blotting and for expression of hLYZ activity by Micrococcal lysis assay. The results showed that expression of the three recombinant vectors was mainly restricted to mammary glands with some degrees of ectopic expression in spleen, intestines and/or kidney.

注射后72 h采集乳样，经微球菌溶解实验证明，3组小鼠乳汁中hLYZ的表达量分别为87、69、60 mg/L；从每组小鼠的12种组织提取总RNA，经Dot blotting检测证明，三种重组载体除在乳腺中表达外，p205C3LYZ还在小鼠的脾和肠、pBJLYZ在脾、pBCLYZ在脾和肾中具有一定的异位表达；在有hLYZ mRNA转录的上述脏器中用微球菌溶解试验均可检测到hLYZ活性。

To study the role of FoxO1 on myoblast proliferation and differentiation and the transformation of muscle fiber type in porcine,here we use yorkshire as experimental materials and detect the expression of genes related myogenesis and muscle fiber type in pporcine myoblast that was knockout or activated FoxO1,adopting the technology of RNAi,real time-PCR and Western blotting et al..

为探讨FoxO1对猪成肌细胞增殖分化及肌纤维类型转化的作用及机理，本试验以大白猪为研究材料，利用RNAi、real-time PCR、免疫细胞化学和Western blotting等技术，通过在原代培养的猪成肌细胞中敲除和激活内源活性FoxO1，检测肌肉形成与肌纤维类型相关基因的表达。

Osteoblasts were detected by alkaline phosphatese and calcium salt staining. The expression of osteopontin was measured by Western blotting.

成骨细胞鉴定采用组织化学方法行碱性磷酸酶和钙化斑块染色同时应用Western blotting法测定骨桥蛋白表达。

The DNA of Yak PrP expressed plasmid extracted from the recombinant expressed bacterium was identified by PCR amplification and two-ezyme digestion. The expressed product was obtained from the recombinant bacterium inducted under the optimized expressed condition (37℃, 1 mmol/L IPTG, 6 h), and it was determined by SDS-PAGE and Western blotting. The GST-BoPrP(23—242) fusion proteins were collected by two ways: the first is to purify and renature from the inclusion bodies; the second is to separate by SDS-PAGE.

将保存的含有牦牛PrP基因重组表达菌提取质粒DNA，进行PCR扩增和双酶切鉴定，并在优化诱导表达条件(37℃，1mmol/L IPTG，6h)下，获得的表达产物进行SDS-PAGE和Western blotting检测；将鉴定之后的重组表达菌诱导表达后，通过两种方法回收GST-BoPrP(23～242)融合蛋白：其一，是从包涵体中提取和复性GST-BoPrP(23～242)融合蛋白；其二，是通过SDS-PAGE直接分离并纯化GST-BoPrP(23～242)融合蛋白。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐